Engineering Microbiology, Год журнала: 2024, Номер unknown, С. 100179 - 100179
Опубликована: Ноя. 1, 2024
Язык: Английский
Communications Chemistry, Год журнала: 2024, Номер 7(1)
Опубликована: Июнь 18, 2024
Abstract Oligonucleotides are advancing as essential materials for the development of new therapeutics, artificial genes, or in storage information applications. Hitherto, our capacity to write (i.e., synthesize) oligonucleotides is not efficient that read sequencing) DNA/RNA. Alternative, biocatalytic methods de novo synthesis natural modified dire need circumvent limitations traditional synthetic approaches. This Perspective article summarizes recent progress made controlled enzymatic synthesis, where temporary blocked nucleotides incorporated into immobilized primers by polymerases. While robust protocols have been established DNA, RNA XNA more challenging. Nevertheless, using a suitable combination protected and polymerase has shown promises produce even though production long DNA/RNA/XNA sequences (>1000 nt) remains We surmise merging ligase- polymerase-based would help current shortcomings synthesis.
Язык: Английский
Процитировано
14
Advanced Materials, Год журнала: 2024, Номер 36(26)
Опубликована: Март 16, 2024
Abstract Existing tear sensors are difficult to perform multiplexed assays due the minute amounts of biomolecules in tears and tiny volume tears. Herein, authors leverage DNA tetrahedral frameworks (DTFs) modified on wireless portable electrodes effectively capture 3D hybridization chain reaction (HCR) amplifiers for automatic sensitive monitoring multiple cytokines human The developed allow determination various dry eye syndrome (DES)‐associated with limit detection down 0.1 pg mL −1 , consuming as little 3 fluid. Double‐blind testing clinical DES samples using sensor commercial ELISA shows no significant difference between them. Compared single‐biomarker diagnosis, diagnostic accuracy this based biomarkers has improved by ≈16%. system offers potential enable personalized accurate diagnosis ocular diseases.
Язык: Английский
Процитировано
7
Biotechnology Advances, Год журнала: 2024, Номер 76, С. 108436 - 108436
Опубликована: Авг. 28, 2024
Язык: Английский
Процитировано
4
Science Advances, Год журнала: 2024, Номер 10(35)
Опубликована: Авг. 30, 2024
The transport of ions through biological ion channels is regulated not only by their structural characteristics but also the composition phospholipid membrane, which serves as a carrier for nanochannels. Inspired modulation currents lipid membrane composition, exemplified activation K
Язык: Английский
Процитировано
3
ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown
Опубликована: Март 21, 2025
Nucleic acid aptamers, known as chemical antibodies, demonstrate remarkable affinity and specificity for targets. Therefore, aptamers are proposed an alternative to antibody in extensive applications. However, nucleic exhibit poor tolerance degradation by nucleases, which severely hampers their biological Herein, we developed a regulation pattern utilizing small-molecule-mediated terminal manipulation, could prevent the interaction of DNA with exonucleases help persist desired conformation high stability. Diagonal T-T bases were designed ends be chemically cross-linked trioxsalen via photocatalyzed cycloaddition. Aptamers different patterns cross-linking sites synthesized. Experimental investigation molecular dynamics simulations combinedly revealed that efficiency depended on multiple factors: (i) number sequences, (ii) spatial (iii) competitive binding ability compared other base sites. The locked exhibited superior exonuclease resistance, especially both 3'- 5'-cross-linked ends, thus demonstrating great target capability. Notably, application exploration, bound receptor-binding domains SARS-CoV-2, showed performance virus infection inhibition. This work puts forward paradigm develop based manipulation DNA, potentially promoting clinical applications drugs.
Язык: Английский
Процитировано
0
ACS Applied Bio Materials, Год журнала: 2025, Номер unknown
Опубликована: Апрель 1, 2025
In-situ fabrication of nucleic acid molecular machines in biological environments is desirable for smart theranostic applications. However, given the complex nature environments, integration multiple functional modules into a coordinated machine remains challenging. Recent advances nanotechnology offer solutions to these challenges. Here, we outline design principles acid–based tailored physiological conditions, drawing on recent examples. We review cutting-edge technologies that facilitate their functionalization settings, particularly presynthesis modifications using unnatural bases and postsynthesis via bioorthogonal chemistry noncovalent interactions. discuss advantages limitations suggest future directions overcome existing
Язык: Английский
Процитировано
0
Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108604 - 108604
Опубликована: Май 1, 2025
Язык: Английский
Процитировано
0
Communications Chemistry, Год журнала: 2025, Номер 8(1)
Опубликована: Май 13, 2025
Язык: Английский
Процитировано
0
bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown
Опубликована: Май 15, 2025
Three-way junctions are simple and fundamental structural motifs that impart the typical branching property in most DNA nanostructures. While conventional three-way well-known, mesojunctions relatively unexplored. Here, we report synthesis of peptide nucleic acid (PNA)/DNA hybrid mesojunctions, containing a 14 bp duplex arm two PNA/DNA arms. We show mesojunction can be assembled magnesium-free buffers low concentrations calcium or sodium salts. showed higher thermal stability compared to versions. Further, exhibited nuclease resistance against DNase I. Our results pave way for integrated into complex nanostructure designs improve their enzymatic stability.
Язык: Английский
Процитировано
0
Nature Protocols, Год журнала: 2025, Номер unknown
Опубликована: Май 26, 2025
Язык: Английский
Процитировано
0