Terminal Deoxynucleotidyl Transferase: Properties and Applications DOI Creative Commons
Chengjie Zhang, Hizar Subthain,

Fei Guo

и другие.

Engineering Microbiology, Год журнала: 2024, Номер unknown, С. 100179 - 100179

Опубликована: Ноя. 1, 2024

Язык: Английский

Controlled enzymatic synthesis of oligonucleotides DOI Creative Commons
Maëva Pichon, Marcel Hollenstein

Communications Chemistry, Год журнала: 2024, Номер 7(1)

Опубликована: Июнь 18, 2024

Abstract Oligonucleotides are advancing as essential materials for the development of new therapeutics, artificial genes, or in storage information applications. Hitherto, our capacity to write (i.e., synthesize) oligonucleotides is not efficient that read sequencing) DNA/RNA. Alternative, biocatalytic methods de novo synthesis natural modified dire need circumvent limitations traditional synthetic approaches. This Perspective article summarizes recent progress made controlled enzymatic synthesis, where temporary blocked nucleotides incorporated into immobilized primers by polymerases. While robust protocols have been established DNA, RNA XNA more challenging. Nevertheless, using a suitable combination protected and polymerase has shown promises produce even though production long DNA/RNA/XNA sequences (>1000 nt) remains We surmise merging ligase- polymerase-based would help current shortcomings synthesis.

Язык: Английский

Процитировано

14

Framework Nucleic Acids Combined with 3D Hybridization Chain Reaction Amplifiers for Monitoring Multiple Human Tear Cytokines DOI
Xing Lü, Xujiao Zhou, Bin Song

и другие.

Advanced Materials, Год журнала: 2024, Номер 36(26)

Опубликована: Март 16, 2024

Abstract Existing tear sensors are difficult to perform multiplexed assays due the minute amounts of biomolecules in tears and tiny volume tears. Herein, authors leverage DNA tetrahedral frameworks (DTFs) modified on wireless portable electrodes effectively capture 3D hybridization chain reaction (HCR) amplifiers for automatic sensitive monitoring multiple cytokines human The developed allow determination various dry eye syndrome (DES)‐associated with limit detection down 0.1 pg mL −1 , consuming as little 3 fluid. Double‐blind testing clinical DES samples using sensor commercial ELISA shows no significant difference between them. Compared single‐biomarker diagnosis, diagnostic accuracy this based biomarkers has improved by ≈16%. system offers potential enable personalized accurate diagnosis ocular diseases.

Язык: Английский

Процитировано

7

Recent advances in the construction strategy, functional properties, and biosensing application of self-assembled triangular unit-based DNA nanostructures DOI

Mengxia Duan,

Yu‐Ting Chang, Xiaowan Chen

и другие.

Biotechnology Advances, Год журнала: 2024, Номер 76, С. 108436 - 108436

Опубликована: Авг. 28, 2024

Язык: Английский

Процитировано

4

Precise control of transmembrane current via regulating bionic lipid membrane composition DOI Creative Commons
Zhiwei Shang, Jing Zhao, Mengyu Yang

и другие.

Science Advances, Год журнала: 2024, Номер 10(35)

Опубликована: Авг. 30, 2024

The transport of ions through biological ion channels is regulated not only by their structural characteristics but also the composition phospholipid membrane, which serves as a carrier for nanochannels. Inspired modulation currents lipid membrane composition, exemplified activation K

Язык: Английский

Процитировано

3

DNA Aptamers with Chemically Locked Ends for Virus Infection Inhibition DOI
Wenzhe Song,

Chuanxi Li,

Yuhang Dong

и другие.

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Март 21, 2025

Nucleic acid aptamers, known as chemical antibodies, demonstrate remarkable affinity and specificity for targets. Therefore, aptamers are proposed an alternative to antibody in extensive applications. However, nucleic exhibit poor tolerance degradation by nucleases, which severely hampers their biological Herein, we developed a regulation pattern utilizing small-molecule-mediated terminal manipulation, could prevent the interaction of DNA with exonucleases help persist desired conformation high stability. Diagonal T-T bases were designed ends be chemically cross-linked trioxsalen via photocatalyzed cycloaddition. Aptamers different patterns cross-linking sites synthesized. Experimental investigation molecular dynamics simulations combinedly revealed that efficiency depended on multiple factors: (i) number sequences, (ii) spatial (iii) competitive binding ability compared other base sites. The locked exhibited superior exonuclease resistance, especially both 3'- 5'-cross-linked ends, thus demonstrating great target capability. Notably, application exploration, bound receptor-binding domains SARS-CoV-2, showed performance virus infection inhibition. This work puts forward paradigm develop based manipulation DNA, potentially promoting clinical applications drugs.

Язык: Английский

Процитировано

0

Functionalization of Nucleic Acid Molecular Machines under Physiological Conditions: A Review DOI
Mo Zhou,

Hongzhen Peng,

Shihua Luo

и другие.

ACS Applied Bio Materials, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

In-situ fabrication of nucleic acid molecular machines in biological environments is desirable for smart theranostic applications. However, given the complex nature environments, integration multiple functional modules into a coordinated machine remains challenging. Recent advances nanotechnology offer solutions to these challenges. Here, we outline design principles acid–based tailored physiological conditions, drawing on recent examples. We review cutting-edge technologies that facilitate their functionalization settings, particularly presynthesis modifications using unnatural bases and postsynthesis via bioorthogonal chemistry noncovalent interactions. discuss advantages limitations suggest future directions overcome existing

Язык: Английский

Процитировано

0

Enzymatic de novo oligonucleotide synthesis: Emerging techniques and advancements DOI Creative Commons

Nanfeng Gao,

Aimiao Yu,

Weikang Yang

и другие.

Biotechnology Advances, Год журнала: 2025, Номер unknown, С. 108604 - 108604

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0

An epimer of threose nucleic acid enhances oligonucleotide exonuclease resistance through end capping DOI Creative Commons
Junlin Wen, Chunlei Zhang, Xuebo Chen

и другие.

Communications Chemistry, Год журнала: 2025, Номер 8(1)

Опубликована: Май 13, 2025

Язык: Английский

Процитировано

0

Peptide nucleic acid (PNA) and DNA hybrid three-way junctions and mesojunctions DOI

Bharath Raj Madhanagopal,

Akul Patel,

Hannah Talbot

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Май 15, 2025

Three-way junctions are simple and fundamental structural motifs that impart the typical branching property in most DNA nanostructures. While conventional three-way well-known, mesojunctions relatively unexplored. Here, we report synthesis of peptide nucleic acid (PNA)/DNA hybrid mesojunctions, containing a 14 bp duplex arm two PNA/DNA arms. We show mesojunction can be assembled magnesium-free buffers low concentrations calcium or sodium salts. showed higher thermal stability compared to versions. Further, exhibited nuclease resistance against DNase I. Our results pave way for integrated into complex nanostructure designs improve their enzymatic stability.

Язык: Английский

Процитировано

0

Hybridization chain reaction-based DNA nanoframeworks for biosensing and therapeutic applications DOI

Zhaoyue Lv,

Peiran Li, Mingxing Liu

и другие.

Nature Protocols, Год журнала: 2025, Номер unknown

Опубликована: Май 26, 2025

Язык: Английский

Процитировано

0