Terminal Deoxynucleotidyl Transferase: Properties and Applications DOI Creative Commons
Chengjie Zhang, Hizar Subthain,

Fei Guo

et al.

Engineering Microbiology, Journal Year: 2024, Volume and Issue: unknown, P. 100179 - 100179

Published: Nov. 1, 2024

Language: Английский

Controlled enzymatic synthesis of oligonucleotides DOI Creative Commons
Maëva Pichon, Marcel Hollenstein

Communications Chemistry, Journal Year: 2024, Volume and Issue: 7(1)

Published: June 18, 2024

Abstract Oligonucleotides are advancing as essential materials for the development of new therapeutics, artificial genes, or in storage information applications. Hitherto, our capacity to write (i.e., synthesize) oligonucleotides is not efficient that read sequencing) DNA/RNA. Alternative, biocatalytic methods de novo synthesis natural modified dire need circumvent limitations traditional synthetic approaches. This Perspective article summarizes recent progress made controlled enzymatic synthesis, where temporary blocked nucleotides incorporated into immobilized primers by polymerases. While robust protocols have been established DNA, RNA XNA more challenging. Nevertheless, using a suitable combination protected and polymerase has shown promises produce even though production long DNA/RNA/XNA sequences (>1000 nt) remains We surmise merging ligase- polymerase-based would help current shortcomings synthesis.

Language: Английский

Citations

14
Framework Nucleic Acids Combined with 3D Hybridization Chain Reaction Amplifiers for Monitoring Multiple Human Tear Cytokines DOI
Xing Lü, Xujiao Zhou, Bin Song

et al.

Advanced Materials, Journal Year: 2024, Volume and Issue: 36(26)

Published: March 16, 2024

Abstract Existing tear sensors are difficult to perform multiplexed assays due the minute amounts of biomolecules in tears and tiny volume tears. Herein, authors leverage DNA tetrahedral frameworks (DTFs) modified on wireless portable electrodes effectively capture 3D hybridization chain reaction (HCR) amplifiers for automatic sensitive monitoring multiple cytokines human The developed allow determination various dry eye syndrome (DES)‐associated with limit detection down 0.1 pg mL −1 , consuming as little 3 fluid. Double‐blind testing clinical DES samples using sensor commercial ELISA shows no significant difference between them. Compared single‐biomarker diagnosis, diagnostic accuracy this based biomarkers has improved by ≈16%. system offers potential enable personalized accurate diagnosis ocular diseases.

Language: Английский

Citations

7
Recent advances in the construction strategy, functional properties, and biosensing application of self-assembled triangular unit-based DNA nanostructures DOI

Mengxia Duan,

Yu‐Ting Chang, Xiaowan Chen

et al.

Biotechnology Advances, Journal Year: 2024, Volume and Issue: 76, P. 108436 - 108436

Published: Aug. 28, 2024

Language: Английский

Citations

4
Precise control of transmembrane current via regulating bionic lipid membrane composition DOI Creative Commons
Zhiwei Shang, Jing Zhao, Mengyu Yang

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(35)

Published: Aug. 30, 2024

The transport of ions through biological ion channels is regulated not only by their structural characteristics but also the composition phospholipid membrane, which serves as a carrier for nanochannels. Inspired modulation currents lipid membrane composition, exemplified activation K

Language: Английский

Citations

3
DNA Aptamers with Chemically Locked Ends for Virus Infection Inhibition DOI
Wenzhe Song,

Chuanxi Li,

Yuhang Dong

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 21, 2025

Nucleic acid aptamers, known as chemical antibodies, demonstrate remarkable affinity and specificity for targets. Therefore, aptamers are proposed an alternative to antibody in extensive applications. However, nucleic exhibit poor tolerance degradation by nucleases, which severely hampers their biological Herein, we developed a regulation pattern utilizing small-molecule-mediated terminal manipulation, could prevent the interaction of DNA with exonucleases help persist desired conformation high stability. Diagonal T-T bases were designed ends be chemically cross-linked trioxsalen via photocatalyzed cycloaddition. Aptamers different patterns cross-linking sites synthesized. Experimental investigation molecular dynamics simulations combinedly revealed that efficiency depended on multiple factors: (i) number sequences, (ii) spatial (iii) competitive binding ability compared other base sites. The locked exhibited superior exonuclease resistance, especially both 3'- 5'-cross-linked ends, thus demonstrating great target capability. Notably, application exploration, bound receptor-binding domains SARS-CoV-2, showed performance virus infection inhibition. This work puts forward paradigm develop based manipulation DNA, potentially promoting clinical applications drugs.

Language: Английский

Citations

0
Functionalization of Nucleic Acid Molecular Machines under Physiological Conditions: A Review DOI
Mo Zhou,

Hongzhen Peng,

Shihua Luo

et al.

ACS Applied Bio Materials, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

In-situ fabrication of nucleic acid molecular machines in biological environments is desirable for smart theranostic applications. However, given the complex nature environments, integration multiple functional modules into a coordinated machine remains challenging. Recent advances nanotechnology offer solutions to these challenges. Here, we outline design principles acid–based tailored physiological conditions, drawing on recent examples. We review cutting-edge technologies that facilitate their functionalization settings, particularly presynthesis modifications using unnatural bases and postsynthesis via bioorthogonal chemistry noncovalent interactions. discuss advantages limitations suggest future directions overcome existing

Language: Английский

Citations

0
Enzymatic de novo oligonucleotide synthesis: Emerging techniques and advancements DOI Creative Commons

Nanfeng Gao,

Aimiao Yu,

Weikang Yang

et al.

Biotechnology Advances, Journal Year: 2025, Volume and Issue: unknown, P. 108604 - 108604

Published: May 1, 2025

Language: Английский

Citations

0
An epimer of threose nucleic acid enhances oligonucleotide exonuclease resistance through end capping DOI Creative Commons
Junlin Wen, Chunlei Zhang, Xuebo Chen

et al.

Communications Chemistry, Journal Year: 2025, Volume and Issue: 8(1)

Published: May 13, 2025

Language: Английский

Citations

0
Peptide nucleic acid (PNA) and DNA hybrid three-way junctions and mesojunctions DOI

Bharath Raj Madhanagopal,

Akul Patel,

Hannah Talbot

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

Three-way junctions are simple and fundamental structural motifs that impart the typical branching property in most DNA nanostructures. While conventional three-way well-known, mesojunctions relatively unexplored. Here, we report synthesis of peptide nucleic acid (PNA)/DNA hybrid mesojunctions, containing a 14 bp duplex arm two PNA/DNA arms. We show mesojunction can be assembled magnesium-free buffers low concentrations calcium or sodium salts. showed higher thermal stability compared to versions. Further, exhibited nuclease resistance against DNase I. Our results pave way for integrated into complex nanostructure designs improve their enzymatic stability.

Language: Английский

Citations

0
Hybridization chain reaction-based DNA nanoframeworks for biosensing and therapeutic applications DOI

Zhaoyue Lv,

Peiran Li, Mingxing Liu

et al.

Nature Protocols, Journal Year: 2025, Volume and Issue: unknown

Published: May 26, 2025

Language: Английский

Citations

0