Analytical Chemistry, Год журнала: 2024, Номер unknown
Опубликована: Сен. 12, 2024
DNA-templated reactions have found wide applications in sensing and drug discovery. However, this strategy has been limited to the use of nucleic acids as templating elements direct proximity effect. Herein, we describe a versatile
Язык: Английский
Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(16)
Опубликована: Янв. 23, 2024
Abstract Systematic evolution of ligands by exponential enrichment (SELEX) has been used to discover thousands aptamers since its development in 1990. Aptamers are short single‐stranded oligonucleotides capable binding targets with high specificity and selectivity through structural recognition. While offer advantages over other molecular recognition elements such as their ease production, smaller size, extended shelf‐life, lower immunogenicity, they have yet show significant success real‐world applications. By analyzing the importance structured library designs, reviewing different SELEX methodologies, effects chemical modifications, we provide a comprehensive overview on production for applications drug delivery systems, therapeutics, diagnostics, imaging.
Язык: Английский
Процитировано
53
Angewandte Chemie International Edition, Год журнала: 2022, Номер 61(50)
Опубликована: Сен. 30, 2022
Nucleic acids are remarkable molecules. In addition to Watson-Crick base pairing, the different structural motifs of these molecules can bind non-nucleic acid targets or catalyze chemical reactions. Additionally, nucleic easily modified with functional groups. These properties make acids, particularly DNA, ideally suited for use in electrochemical biosensors, both as biorecognition elements and redox reporter probes. this Minireview, we will review historical evolution probes biosensors. We then specific examples nucleic-acid-based biosensors that have been evaluated clinical areas infectious disease, cancer, cardiovascular health.
Язык: Английский
Процитировано
54
Analysis & Sensing, Год журнала: 2022, Номер 2(5)
Опубликована: Апрель 21, 2022
The SARS-CoV-2 virus and COVID-19 pandemic continue to demand effective diagnostic therapeutic solutions. Finding these solutions requires highly functional molecular recognition elements. Nucleic acid aptamers represent a possible solution. Characterized by their high affinity specificity, can be rapidly identified from random-sequence nucleic libraries. Over the past two years, many labs around world have rushed create diverse that target important structural proteins of SARS-CoV-2: spike (S) protein nucleocapsid (N) protein. These led identification show real promise for development tests agents SARS-CoV-2. Herein we review all developments, with special focus on aptasensors detecting include electrochemical optical sensors, lateral flow devices, aptamer-linked immobilized sorbent assays.
Язык: Английский
Процитировано
46
Chemical Society Reviews, Год журнала: 2024, Номер 53(13), С. 6830 - 6859
Опубликована: Янв. 1, 2024
This review presents the recent progress on aptamers that have been explored for SARS-CoV-2 detection and therapeutics, wherein construction principles characteristics of aptamer-based assembly systems are systematically summarized.
Язык: Английский
Процитировано
17
Journal of the American Chemical Society, Год журнала: 2022, Номер 144(51), С. 23465 - 23473
Опубликована: Дек. 15, 2022
Our previously discovered monomeric aptamer for SARS-CoV-2 (MSA52) possesses a universal affinity COVID-19 spike protein variants but is ultimately limited by its ability to bind only one subunit of the protein. The symmetrical shape homotrimeric presents opportunity create matching molecular recognition element that perfectly complementary structural scaffold, causing enhanced binding affinity. Here, we describe branched with three-fold rotational symmetry, named TMSA52, not excellent also capable several picomolar affinity, as well pseudotyped lentiviruses expressing femtomolar Using Pd–Ir nanocubes nanozymes in an enzyme-linked assay (ELABA), TMSA52 was sensitively detecting diverse pooled human saliva limit detection low 6.3 × 103 copies/mL. ELABA used test 50 SARS-CoV-2-positive and 60 SARS-CoV-2-negative patient samples, providing sensitivity specificity values 84.0 98.3%, respectively, thus highlighting potential development future rapid tests.
Язык: Английский
Процитировано
39
Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(51)
Опубликована: Окт. 31, 2023
Abstract Here we report on an ultra‐sensitive colorimetric sensing platform that takes advantage of both the strong amplification power rolling circle (RCA) and high efficiency a simple urease‐mediated litmus test. The presence target triggers RCA reaction, urease‐labelled DNA can hybridize to biotinylated products be immobilized onto streptavidin‐coated magnetic beads. urease‐laden beads are then used hydrolyze urea, leading increase in pH detected by We show this easily integrated with aptamers for diverse targets via detection human thrombin platelet‐derived growth factor (PDGF) utilizing structure‐switching as well SARS‐CoV‐2 saliva using spike‐binding trimeric aptamer. Furthermore, demonstrate into paper‐based device applications.
Язык: Английский
Процитировано
20
Microchimica Acta, Год журнала: 2024, Номер 191(6)
Опубликована: Май 10, 2024
Abstract The COVID-19 pandemic underlines the need for effective strategies controlling virus spread and ensuring sensitive detection of SARS-CoV-2. This review presents potential nanomaterial-enabled optical biosensors rapid low-cost SARS-CoV-2 biomarkers, demonstrating a comprehensive analysis including colorimetric, fluorescence, surface-enhanced Raman scattering, surface plasmon resonance methods. Nanomaterials metal-based nanomaterials, metal–organic frame–based nanoparticles, nanorods, nanoporous materials, nanoshell magnetic nanoparticles employed in production are presented detail. also discusses principles, fabrication methods, nanomaterial synthesis, their applications four categories: antibody-based, antigen-based, nucleic acid–based, aptamer-based biosensors. critical includes reports published literature between years 2021 2024. In addition, offers insights into nanobiosensors diagnosis COVID-19. integration artificial intelligence machine learning technologies with is proposed to improve efficiency diagnostic systems future scenarios. Graphical
Язык: Английский
Процитировано
8
Scientific Reports, Год журнала: 2024, Номер 14(1)
Опубликована: Март 29, 2024
Abstract COVID-19 caused by SARS-CoV-2 spread rapidly around the world, endangering health of people globally. The spike protein initiates entry into target cells binding to human angiotensin-converting enzyme 2 (ACE2). In this study, we developed DNA aptamers that specifically bind protein, thereby inhibiting its ACE2. are small nucleic acid fragments with random structures selectively various molecules. We identified nine targeting using systematic evolution ligands exponential enrichment (SELEX) method and selected three optimal comparing their affinities. Additionally, confirmed suppressed pro-inflammatory cytokines induced in ACE2-overexpressing HEK293 cells. Overall, aptamer study has potential inhibit or block interaction Thus, our can be used as new biological tools for prevention diagnosis infection.
Язык: Английский
Процитировано
7
ACS Sensors, Год журнала: 2023, Номер 8(4), С. 1558 - 1567
Опубликована: Март 17, 2023
Wastewater analysis of pathogens, particularly SARS-CoV-2, is instrumental in tracking and monitoring infectious diseases a population. This method can be used to generate early warnings regarding the onset an disease predict associated infection trends. Currently, wastewater SARS-CoV-2 almost exclusively performed using polymerase chain reaction for amplification-based detection viral RNA at centralized laboratories. Despite development several biosensing technologies offering point-of-care solutions analyzing clinical samples, these remain elusive due low levels virus interference caused by matrix. Herein, we integrate aptamer-based electrochemical chip with filtration, purification, extraction (FPE) system developing alternate in-field solution analysis. The sensing employs dimeric aptamer, which universally applicable wild-type, alpha, delta, omicron variants SARS-CoV-2. We demonstrate that aptamer stable matrix (diluted 50%) its binding affinity not significantly impacted. demonstrates limit 1000 copies/L (1 copy/mL), enabled amplification provided FPE system. allows integrated detect trace amounts native categorize amount contamination into (<10 copies/mL), medium (10–1000 or high (>1000 copies/mL) levels, providing viable use.
Язык: Английский
Процитировано
15
Advanced Science, Год журнала: 2023, Номер 10(22)
Опубликована: Май 19, 2023
Abstract RNA aptamers provide useful biological probes and therapeutic agents. New methodologies to screen will be valuable by complementing the traditional Systematic Evolution of Ligands Exponential Enrichment (SELEX). Meanwhile, repurposing clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated systems (Cas) has expanded their utility far beyond native nuclease function. Here, CRISmers, a CRISPR/Cas‐based novel screening system for based on binding chosen protein interest in cellular context, is presented. Using are identified specifically targeting receptor domain (RBD) spike glycoprotein severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). Two aptamer leads enable sensitive detection potent neutralization SARS‐CoV‐2 Delta Omicron variants vitro. Intranasal administration one aptamer, further modified with 2’‐fluoro pyrimidines (2’‐F), 2’‐ O ‐methyl purines (2’‐O), conjugation both cholesterol polyethylene glycol 40 kDa (PEG40K), achieves effective prophylactic antiviral activity against live BA.2 vivo. The study concludes demonstrating robustness, consistency, potential broad CRISmers using two newly but switching CRISPR, selection marker, host species.
Язык: Английский
Процитировано
13