Angiotensin-Converting Enzyme 2-Based Biosensing Modalities and Devices for Coronavirus Detection DOI Creative Commons
Ijaz Gul,

Shiyao Zhai,

Xiaoyun Zhong

et al.

Biosensors, Journal Year: 2022, Volume and Issue: 12(11), P. 984 - 984

Published: Nov. 7, 2022

Rapid and cost-effective diagnostic tests for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are a critical valuable weapon the disease 2019 (COVID-19) pandemic response. SARS-CoV-2 invasion is primarily mediated by human angiotensin-converting enzyme (hACE2). Recent developments in ACE2-based detection modalities accentuate potential of this natural host-virus interaction developing point-of-care (POC) COVID-19 systems. Although research on harnessing ACE2 its infancy, some interesting biosensing devices have been developed, showing commercial viability intriguing new approach. The exquisite performance reported biosensors provides opportunities researchers to develop rapid tools suitable virus at points entry, workplaces, or congregate scenarios order effectively implement control management plans. However, be considered as an emerging approach, rationale needs critically comprehensively surveyed discussed. Herein, we review recent status methods, signal transduction principles development trend future. We discuss challenges ACE2-biosensors delineate prospects their use, along with recommended solutions suggestions.

Language: Английский

Development of Better Aptamers: Structured Library Approaches, Selection Methods, and Chemical Modifications DOI Creative Commons
Alex Brown,

Jake Brill,

Ryan Amini

et al.

Angewandte Chemie International Edition, Journal Year: 2024, Volume and Issue: 63(16)

Published: Jan. 23, 2024

Abstract Systematic evolution of ligands by exponential enrichment (SELEX) has been used to discover thousands aptamers since its development in 1990. Aptamers are short single‐stranded oligonucleotides capable binding targets with high specificity and selectivity through structural recognition. While offer advantages over other molecular recognition elements such as their ease production, smaller size, extended shelf‐life, lower immunogenicity, they have yet show significant success real‐world applications. By analyzing the importance structured library designs, reviewing different SELEX methodologies, effects chemical modifications, we provide a comprehensive overview on production for applications drug delivery systems, therapeutics, diagnostics, imaging.

Language: Английский

Citations

48
Development of Nucleic‐Acid‐Based Electrochemical Biosensors for Clinical Applications DOI
Zijie Zhang, Payel Sen, Bal Ram Adhikari

et al.

Angewandte Chemie International Edition, Journal Year: 2022, Volume and Issue: 61(50)

Published: Sept. 30, 2022

Nucleic acids are remarkable molecules. In addition to Watson-Crick base pairing, the different structural motifs of these molecules can bind non-nucleic acid targets or catalyze chemical reactions. Additionally, nucleic easily modified with functional groups. These properties make acids, particularly DNA, ideally suited for use in electrochemical biosensors, both as biorecognition elements and redox reporter probes. this Minireview, we will review historical evolution probes biosensors. We then specific examples nucleic-acid-based biosensors that have been evaluated clinical areas infectious disease, cancer, cardiovascular health.

Language: Английский

Citations

54
Aptamers for SARS‐CoV‐2: Isolation, Characterization, and Diagnostic and Therapeutic Developments DOI
Ryan Amini, Zijie Zhang, Jiuxing Li

et al.

Analysis & Sensing, Journal Year: 2022, Volume and Issue: 2(5)

Published: April 21, 2022

The SARS-CoV-2 virus and COVID-19 pandemic continue to demand effective diagnostic therapeutic solutions. Finding these solutions requires highly functional molecular recognition elements. Nucleic acid aptamers represent a possible solution. Characterized by their high affinity specificity, can be rapidly identified from random-sequence nucleic libraries. Over the past two years, many labs around world have rushed create diverse that target important structural proteins of SARS-CoV-2: spike (S) protein nucleocapsid (N) protein. These led identification show real promise for development tests agents SARS-CoV-2. Herein we review all developments, with special focus on aptasensors detecting include electrochemical optical sensors, lateral flow devices, aptamer-linked immobilized sorbent assays.

Language: Английский

Citations

46
Three on Three: Universal and High-Affinity Molecular Recognition of the Symmetric Homotrimeric Spike Protein of SARS-CoV-2 with a Symmetric Homotrimeric Aptamer DOI Creative Commons
Jiuxing Li, Zijie Zhang, Jimmy Gu

et al.

Journal of the American Chemical Society, Journal Year: 2022, Volume and Issue: 144(51), P. 23465 - 23473

Published: Dec. 15, 2022

Our previously discovered monomeric aptamer for SARS-CoV-2 (MSA52) possesses a universal affinity COVID-19 spike protein variants but is ultimately limited by its ability to bind only one subunit of the protein. The symmetrical shape homotrimeric presents opportunity create matching molecular recognition element that perfectly complementary structural scaffold, causing enhanced binding affinity. Here, we describe branched with three-fold rotational symmetry, named TMSA52, not excellent also capable several picomolar affinity, as well pseudotyped lentiviruses expressing femtomolar Using Pd–Ir nanocubes nanozymes in an enzyme-linked assay (ELABA), TMSA52 was sensitively detecting diverse pooled human saliva limit detection low 6.3 × 103 copies/mL. ELABA used test 50 SARS-CoV-2-positive and 60 SARS-CoV-2-negative patient samples, providing sensitivity specificity values 84.0 98.3%, respectively, thus highlighting potential development future rapid tests.

Language: Английский

Citations

39
Aptamer-based assembly systems for SARS-CoV-2 detection and therapeutics DOI

Yuhang Dong,

Jingping Wang,

Ling Chen

et al.

Chemical Society Reviews, Journal Year: 2024, Volume and Issue: 53(13), P. 6830 - 6859

Published: Jan. 1, 2024

This review presents the recent progress on aptamers that have been explored for SARS-CoV-2 detection and therapeutics, wherein construction principles characteristics of aptamer-based assembly systems are systematically summarized.

Language: Английский

Citations

16
A Colorimetric Biosensing Platform with Aptamers, Rolling Circle Amplification and Urease‐Mediated Litmus Test DOI Creative Commons
Dingran Chang, Jiuxing Li, Rudi Liu

et al.

Angewandte Chemie International Edition, Journal Year: 2023, Volume and Issue: 62(51)

Published: Oct. 31, 2023

Abstract Here we report on an ultra‐sensitive colorimetric sensing platform that takes advantage of both the strong amplification power rolling circle (RCA) and high efficiency a simple urease‐mediated litmus test. The presence target triggers RCA reaction, urease‐labelled DNA can hybridize to biotinylated products be immobilized onto streptavidin‐coated magnetic beads. urease‐laden beads are then used hydrolyze urea, leading increase in pH detected by We show this easily integrated with aptamers for diverse targets via detection human thrombin platelet‐derived growth factor (PDGF) utilizing structure‐switching as well SARS‐CoV‐2 saliva using spike‐binding trimeric aptamer. Furthermore, demonstrate into paper‐based device applications.

Language: Английский

Citations

19
A new DNA aptamer which binds to SARS-CoV-2 spike protein and reduces pro-inflammatory response DOI Creative Commons
Woong Kim,

Eun Su Song,

Song Ha Lee

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 29, 2024

Abstract COVID-19 caused by SARS-CoV-2 spread rapidly around the world, endangering health of people globally. The spike protein initiates entry into target cells binding to human angiotensin-converting enzyme 2 (ACE2). In this study, we developed DNA aptamers that specifically bind protein, thereby inhibiting its ACE2. are small nucleic acid fragments with random structures selectively various molecules. We identified nine targeting using systematic evolution ligands exponential enrichment (SELEX) method and selected three optimal comparing their affinities. Additionally, confirmed suppressed pro-inflammatory cytokines induced in ACE2-overexpressing HEK293 cells. Overall, aptamer study has potential inhibit or block interaction Thus, our can be used as new biological tools for prevention diagnosis infection.

Language: Английский

Citations

7
Optical biosensors for diagnosis of COVID-19: nanomaterial-enabled particle strategies for post pandemic era DOI Creative Commons

Yusuf Şamil Tekin,

Seyda Mihriban Kul,

Osman Sağdıç

et al.

Microchimica Acta, Journal Year: 2024, Volume and Issue: 191(6)

Published: May 10, 2024

Abstract The COVID-19 pandemic underlines the need for effective strategies controlling virus spread and ensuring sensitive detection of SARS-CoV-2. This review presents potential nanomaterial-enabled optical biosensors rapid low-cost SARS-CoV-2 biomarkers, demonstrating a comprehensive analysis including colorimetric, fluorescence, surface-enhanced Raman scattering, surface plasmon resonance methods. Nanomaterials metal-based nanomaterials, metal–organic frame–based nanoparticles, nanorods, nanoporous materials, nanoshell magnetic nanoparticles employed in production are presented detail. also discusses principles, fabrication methods, nanomaterial synthesis, their applications four categories: antibody-based, antigen-based, nucleic acid–based, aptamer-based biosensors. critical includes reports published literature between years 2021 2024. In addition, offers insights into nanobiosensors diagnosis COVID-19. integration artificial intelligence machine learning technologies with is proposed to improve efficiency diagnostic systems future scenarios. Graphical

Language: Английский

Citations

7
Integrating Water Purification with Electrochemical Aptamer Sensing for Detecting SARS-CoV-2 in Wastewater DOI Open Access
Payel Sen, Zijie Zhang, Phoebe Li

et al.

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(4), P. 1558 - 1567

Published: March 17, 2023

Wastewater analysis of pathogens, particularly SARS-CoV-2, is instrumental in tracking and monitoring infectious diseases a population. This method can be used to generate early warnings regarding the onset an disease predict associated infection trends. Currently, wastewater SARS-CoV-2 almost exclusively performed using polymerase chain reaction for amplification-based detection viral RNA at centralized laboratories. Despite development several biosensing technologies offering point-of-care solutions analyzing clinical samples, these remain elusive due low levels virus interference caused by matrix. Herein, we integrate aptamer-based electrochemical chip with filtration, purification, extraction (FPE) system developing alternate in-field solution analysis. The sensing employs dimeric aptamer, which universally applicable wild-type, alpha, delta, omicron variants SARS-CoV-2. We demonstrate that aptamer stable matrix (diluted 50%) its binding affinity not significantly impacted. demonstrates limit 1000 copies/L (1 copy/mL), enabled amplification provided FPE system. allows integrated detect trace amounts native categorize amount contamination into (<10 copies/mL), medium (10–1000 or high (>1000 copies/mL) levels, providing viable use.

Language: Английский

Citations

15
Development of Better Aptamers: Structured Library Approaches, Selection Methods, and Chemical Modifications DOI Creative Commons
Alex Brown,

J. Brill,

Ryan Amini

et al.

Angewandte Chemie, Journal Year: 2024, Volume and Issue: 136(16)

Published: Jan. 23, 2024

Abstract Systematic evolution of ligands by exponential enrichment (SELEX) has been used to discover thousands aptamers since its development in 1990. Aptamers are short single‐stranded oligonucleotides capable binding targets with high specificity and selectivity through structural recognition. While offer advantages over other molecular recognition elements such as their ease production, smaller size, extended shelf‐life, lower immunogenicity, they have yet show significant success real‐world applications. By analyzing the importance structured library designs, reviewing different SELEX methodologies, effects chemical modifications, we provide a comprehensive overview on production for applications drug delivery systems, therapeutics, diagnostics, imaging.

Language: Английский

Citations

5