Attentional modulation of secondary somatosensory and visual thalamus of mice DOI Open Access
Gordon H Petty, Randy M. Bruno

Опубликована: Окт. 28, 2024

Each sensory modality has its own primary and secondary thalamic nuclei. While the nuclei are well understood to relay information from periphery cortex, role of is elusive. One hypothesis been that may support feature-based attention. If this true, one would also expect activity in different reflect degree which modalities or not behaviorally relevant a task. We trained head-fixed mice attend while ignoring second modality, namely touch ignore vision, vice versa. Arrays were used record simultaneously somatosensory thalamus (POm) visual (LP). In respond tactile stimuli stimuli, POm was robustly activated by largely unresponsive stimuli. A pattern observed when touch, with now more during trials. This explained differences movements (i.e., whisking, licking, pupil dilation) resulting two tasks. Post hoc histological reconstruction array tracks through revealed subregions varied their plasticity. LP exhibited similar phenomena. conclude behavioral training reshapes Secondary relevant, reward-predicting regardless stimulus modality.

Язык: Английский

Brain‐wide mapping of inputs to the mouse lateral posterior (LP/Pulvinar) thalamus–anterior cingulate cortex network DOI

Yi Ning Leow,

Blake Zhou, Heather A. Sullivan

и другие.

The Journal of Comparative Neurology, Год журнала: 2022, Номер 530(11), С. 1992 - 2013

Опубликована: Апрель 6, 2022

Abstract The rodent homolog of the primate pulvinar, lateral posterior (LP) thalamus, is extensively interconnected with multiple cortical areas. While these interactions can span entire LP, subdivisions LP are characterized by differential connections specific regions. In particular, medial has reciprocal frontoparietal areas, including anterior cingulate cortex (ACC). ACC plays an integral role in top‐down sensory processing and attentional regulation, likely exerting some functions via LP. However, little known about how interact, information potentially integrated this network. Here, we address gap employing a projection‐specific monosynaptic rabies tracing strategy to delineate brain‐wide inputs bottom‐up LP→ACC ACC→LP neurons. We find that neurons receive from widespread regions, primary higher order motor also extensive subcortical inputs, particularly intermediate deep layers superior colliculus (SC). Sensory largely arise visual addition, integrate cross‐hemispheric prefrontal as well cortex. Our anatomical mapping LP‐ACC pathways provides roadmap for understanding communicate different sources mediate control visuomotor functions.

Язык: Английский

Процитировано

31
Brain-Wide Projections and Differential Encoding of Prefrontal Neuronal Classes Underlying Learned and Innate Threat Avoidance DOI Creative Commons
Michael W. Gongwer,

Cassandra B. Klune,

João Couto

и другие.

Journal of Neuroscience, Год журнала: 2023, Номер 43(32), С. 5810 - 5830

Опубликована: Июль 25, 2023

To understand how the brain produces behavior, we must elucidate relationships between neuronal connectivity and function. The medial prefrontal cortex (mPFC) is critical for complex functions including decision-making mood. mPFC projection neurons collateralize extensively, but activity brain-wide are poorly understood. We performed whole-brain mapping fiber photometry to better circuits that control threat avoidance in male female mice. Using tissue clearing light sheet fluorescence microscopy (LSFM), mapped axon collaterals of populations project nucleus accumbens (NAc), ventral tegmental area (VTA), or contralateral (cmPFC). present DeepTraCE (deep learning-based tracing with combined enhancement), quantifying bulk-labeled axonal projections images cleared tissue, DeepCOUNT (deep-learning based counting objects via 3D U-net pixel tagging), cell bodies. Anatomical maps produced aligned known patterns revealed class-specific topographic within regions. TRAP2 mice DeepCOUNT, analyzed functional underlying avoidance. PL was most highly connected node connections subsets PL-cPL, PL-NAc, PL-VTA target sites. photometry, found during avoidance, cmPFC NAc-projectors encoded conditioned stimuli, only when action required avoid threats. mPFC-VTA learned not innate behaviors. Together our results new optimized approaches quantitative analysis indicate anatomically defined classes have specialized roles

Язык: Английский

Процитировано

21
The pulvinar as a hub of visual processing and cortical integration DOI Open Access
Nelson Cortes, Hugo Ladret,

Reza Abbas-Farishta

и другие.

Trends in Neurosciences, Год журнала: 2023, Номер 47(2), С. 120 - 134

Опубликована: Дек. 24, 2023

Язык: Английский

Процитировано

21
Attentional modulation of secondary somatosensory and visual thalamus of mice DOI Creative Commons
Gordon H Petty, Randy M. Bruno

eLife, Год журнала: 2024, Номер 13

Опубликована: Июнь 24, 2024

Each sensory modality has its own primary and secondary thalamic nuclei. While the nuclei are well understood to relay information from periphery cortex, role of is elusive. We trained head-fixed mice attend one while ignoring a second modality, namely touch ignore vision, or vice versa. Arrays were used record simultaneously somatosensory thalamus (POm) visual (LP). In respond tactile stimuli stimuli, POm was robustly activated by largely unresponsive stimuli. A different pattern observed when touch, with now more during trials. This activity not explained differences in movements (i.e. whisking, licking, pupil dilation) resulting two tasks. Post hoc histological reconstruction array tracks through revealed that subregions varied their degree plasticity. LP exhibited similar phenomena. conclude behavioral training reshapes Secondary same behaviorally relevant, reward-predicting regardless stimulus modality.

Язык: Английский

Процитировано

6
The mouse claustrum synaptically connects cortical network motifs DOI Creative Commons
Houman Qadir, Brent W. Stewart, Jonathan W. VanRyzin

и другие.

Cell Reports, Год журнала: 2022, Номер 41(12), С. 111860 - 111860

Опубликована: Дек. 1, 2022

Язык: Английский

Процитировано

20
Detection of early proteomic alterations in 5xFAD Alzheimer's disease neonatal mouse model via MALDI‐MSI DOI Creative Commons
Irep Uras, Merve Karayel‐Basar,

Betül Şahin

и другие.

Alzheimer s & Dementia, Год журнала: 2023, Номер 19(10), С. 4572 - 4589

Опубликована: Март 18, 2023

Alzheimer's disease (AD) is a debilitating neurodegenerative disorder, characterized by memory deficit and dementia. AD considered multifactorial disorder where multiple processes like amyloid-beta tau accumulation, axonal degeneration, synaptic plasticity, autophagic plays an important role. In this study, the spatial proteomic differences in neonatal 5xFAD brain tissue were investigated using MALDI-MSI coupled to LC-MS/MS, statistically significantly altered proteins associated with AD. Thirty-five differentially expressed (DEPs) between tissues of their littermate mice detected via technique. Among 35 identified, 26 them directly Our results indicated remarkable resemblance protein expression profiles when compared patient specimens or mouse models. These findings showed that molecular alterations existed even at birth some are presages brain. HIGHLIGHTS: Spatial littermate. out (AD). Molecular Alterations function early common neurobiological thread.

Язык: Английский

Процитировано

12
Multi‐atlas thalamic nuclei segmentation on standard T1‐weighed MRI with application to normal aging DOI Creative Commons
Adolf Pfefferbaum, Edith V. Sullivan, Natalie M. Zahr

и другие.

Human Brain Mapping, Год журнала: 2022, Номер 44(2), С. 612 - 628

Опубликована: Окт. 1, 2022

Abstract Specific thalamic nuclei are implicated in healthy aging and age‐related neurodegenerative diseases. However, few methods available for robust automated segmentation of nuclei. The threefold aims this study were to validate the use a modified method on standard T1 MRI data, apply quantify volume declines, test functional meaningfulness by predicting performance motor testing. A version THalamus Optimized Multi‐Atlas Segmentation (THOMAS) generated 22 unilateral For validation, we compared nuclear volumes obtained from THOMAS parcellation white‐matter‐nulled (WMn) data 45 participants. To examine effects age/sex volumes, second set 121 men 117 women, ages 20–86 years, segmented using THOMAS. ramifications, composite regions constituent correlated with Grooved Pegboard scores. showed significant quantitative agreement WMn especially larger Sex differences revealing than women accounted adjustment supratentorial intracranial (sICV). Significant sICV‐adjusted correlations between age detected 20 whole thalamus. Composite Posterior Ventral Anterior/Pulvinar selectively higher scores eye‐hand coordination task. These results support T1‐weighted as adequately parcellation.

Язык: Английский

Процитировано

19
Neuromodulation of striatal D1 cells shapes BOLD fluctuations in anatomically connected thalamic and cortical regions DOI Creative Commons
Marija Markicevic, Oliver Sturman, Johannes Bohacek

и другие.

eLife, Год журнала: 2023, Номер 12

Опубликована: Окт. 12, 2023

Understanding how the brain's macroscale dynamics are shaped by underlying microscale mechanisms is a key problem in neuroscience. In animal models, we can now investigate this relationship unprecedented detail directly manipulating cellular-level properties while measuring whole-brain response using resting-state fMRI. Here, focused on understanding blood-oxygen-level-dependent (BOLD) dynamics, measured within structurally well-defined striato-thalamo-cortical circuit mice, chemogenetically exciting or inhibiting D1 medium spiny neurons (MSNs) of right dorsomedial caudate putamen (CPdm). We characterize changes both BOLD individual cortical and subcortical brain areas, patterns inter-regional coupling (functional connectivity) between pairs areas. Using classification approach based large diverse set time-series properties, found that CPdm neuromodulation alters thalamic subregions project back to striatum. cortex, local were strongest unimodal regions (which process information from single sensory modality) weakened along hierarchical gradient towards transmodal regions. contrast, decrease functional connectivity was observed only for cortico-striatal connections after excitation. Our results show targeted manipulations affect at macroscale, such as making more predictable over time increasing its self-correlation structure. This contributes ongoing attempts understand influence structure-function relationships shaping communication levels.

Язык: Английский

Процитировано

10
Attentional modulation of secondary somatosensory and visual thalamus of mice DOI Creative Commons
Gordon H Petty, Randy M. Bruno

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 25, 2024

ABSTRACT Each sensory modality has its own primary and secondary thalamic nuclei. While the nuclei are well understood to relay information from periphery cortex, role of is elusive. One hypothesis been that may support feature-based attention. If this true, one would also expect activity in different reflect degree which modalities or not behaviorally relevant a task. We trained head-fixed mice attend while ignoring second modality, namely touch ignore vision, vice versa. Arrays were used record simultaneously somatosensory thalamus (POm) visual (LP). In respond tactile stimuli stimuli, POm was robustly activated by largely unresponsive stimuli. A pattern observed when touch, with now more during trials. This explained differences movements (i.e., whisking, licking, pupil dilation) resulting two tasks. Post hoc histological reconstruction array tracks through revealed subregions varied their plasticity. LP exhibited similar phenomena. conclude behavioral training reshapes Secondary relevant, reward-predicting regardless stimulus modality.

Язык: Английский

Процитировано

3
Reconfiguration of Behavioral Signals in the Anterior Cingulate Cortex based on Emotional State DOI

Adrian Lindsay,

Isabella Gallello,

Barak F. Caracheo

и другие.

Journal of Neuroscience, Год журнала: 2024, Номер 44(23), С. e1670232024 - e1670232024

Опубликована: Апрель 18, 2024

Behaviors and their execution depend on the context emotional state in which they are performed. The contextual modulation of behavior likely relies regions such as anterior cingulate cortex (ACC) that multiplex information about emotional/autonomic states behaviors. objective present study was to understand how representations behaviors by ACC neurons become modified when performed different states. A pipeline machine learning techniques developed categorize classify complex, spontaneous male rats from video. This pipeline, termed Hierarchical Unsupervised Behavioural Discovery Tool (HUB-DT), discovered a range statistically separable during task motivationally significant outcomes were delivered blocks trials created three unique contexts. HUB-DT capable detecting specific each able identify segregate portions neural signal related context. Overall, ~10 many responded contextually dependent versus fixed manner, highlighting extreme impact precisely defined based detailed analyses limb kinematics. type may be key mechanism allows modify behavioral output demands.

Язык: Английский

Процитировано

3