Principles of lipid nanoparticle design for mRNA delivery

BMEMat, Год журнала: 2024, Номер unknown

Опубликована: Сен. 19, 2024

Abstract mRNA therapeutics have significantly evolved within the life sciences, particularly in applications such as vaccines, tumor immunotherapy, protein replacement, gene editing, and monoclonal antibody therapy. To fully realize potential of drugs mitigate adverse effects, substantial vector materials been developed for delivery these pharmaceutical agents. Lipid nanoparticles (LNPs) represent most clinically advanced carriers, recognized by U.S. Food Drug Administration approved vaccines numerous clinical trials. Diverse therapeutic necessitate tailored design LNPs. Herein, we outline principles LNP delivery, focusing specifically on their effectiveness, targeting capabilities, safety profiles, nanoparticle stability. Additionally, present latest advancements mRNA‐LNP technology. This review aims to elucidate benefits systems therapeutics, providing insights into breakthroughs innovative ideas further enhancing advantages. These summaries are dedicated promoting broader LNP‐mRNA drugs, aiming advance treatment serious diseases an effective safe manner.

Язык: Английский

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COVID-19 Vaccines, Effectiveness, and Immune Responses

International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(23), С. 15415 - 15415

Опубликована: Дек. 6, 2022

The COVID-19 pandemic, caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has captivated globe's attention since its emergence in 2019. This highly infectious, spreadable, and dangerous pathogen health, social, economic crises. Therefore, a worldwide collaborative effort was made to find an efficient strategy overcome develop vaccines. new vaccines provide effective immune response that safeguards community from virus' severity. WHO approved nine for emergency use based on safety efficacy data collected various conducted clinical trials. Herein, we review effectiveness of WHO-approved associated responses, their impact improving public's health. Several immunological studies have demonstrated vaccination dramatically enhances reduces likelihood future infections previously infected individuals. However, type individual health status can significantly affect responses. Exposure healthy individuals adenovirus vectors or mRNA causes early production antibodies B T cells. On other hand, unhealthy were more likely experience harmful events due relapses existing conditions. Taken together, aligning with proper patient's case result better outcomes.

Язык: Английский

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Recent advances of bioresponsive polymeric nanomedicine for cancer therapy

Nano Research, Год журнала: 2022, Номер 16(2), С. 2660 - 2671

Опубликована: Ноя. 15, 2022

Язык: Английский

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Microfluidic-based technologies for diagnosis, prevention, and treatment of COVID-19: recent advances and future directions

Biomedical Microdevices, Год журнала: 2023, Номер 25(2)

Опубликована: Март 13, 2023

Язык: Английский

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Engineering versatile nano-bacteria hybrids for efficient tumor therapy

Coordination Chemistry Reviews, Год журнала: 2023, Номер 488, С. 215178 - 215178

Опубликована: Апрель 27, 2023

Язык: Английский

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Harnessing Nanovaccines for Effective Immunization─A Special Concern on COVID-19: Facts, Fidelity, and Future Prospective

ACS Biomaterials Science & Engineering, Год журнала: 2023, Номер 10(1), С. 271 - 297

Опубликована: Дек. 14, 2023

Nanotechnology has emerged as a transformative pathway in vaccine research and delivery. Nanovaccines, encompassing lipid nonlipid formulations, exhibit considerable advantages over traditional techniques, including enhanced antigen stability, heightened immunogenicity, targeted distribution, the potential for codelivery with adjuvants or immune modulators. This review provides comprehensive overview of latest advancements applications non-lipid-based nanovaccines current vaccination strategies immunization. The commences by outlining fundamental concepts underlying nanovaccine design before delving into diverse components production processes employed their development. Subsequently, comparative analysis various nanocarriers is presented, elucidating distinct physicochemical characteristics impact on response, along preclinical clinical studies. discussion also highlights how nanotechnology enables possibility personalized combined facilitating creation tailored to meet individual patient needs. ethical aspects concerning use nanovaccines, well safety concerns public perception, are addressed. study underscores gaps challenges that must be overcome adopting practice. offers vital new insights status. It emphasizes significance continuous research, collaboration among interdisciplinary experts, regulatory measures fully unlock enhancing immunization ensuring healthier, more resilient society.

Язык: Английский

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Bovine lactoferrin inhibits SARS‐CoV‐2 and SARS‐CoV‐1 by targeting the RdRp complex and alleviates viral infection in the hamster model

Journal of Medical Virology, Год журнала: 2022, Номер 95(1)

Опубликована: Ноя. 4, 2022

Abstract Breast milk has been found to inhibit coronavirus infection, while the key components and mechanisms are unknown. We aimed determine that contribute antiviral effects of breastmilk explore their potential mechanism. Lactoferrin (Lf) fat globule membrane severe acute respiratory syndrome 2 (SARS‐CoV‐2)‐related GX_P2V transcription‐ replication‐competent SARS‐CoV‐2 virus‐like particles in vitro block viral entry into cells. confirmed bovine Lf (bLf) blocked binding between human angiotensin‐converting enzyme spike protein by combining receptor‐binding domain (RBD). Importantly, bLf inhibited RNA‐dependent RNA polymerase (RdRp) activity both SARS‐CoV nanomolar range. So far, no biological macromolecules have reported RdRp. Our result indicated plays a major role inhibiting replication. treatment reduced load lungs tracheae alleviated pathological damage. study provides evidence prevents infection RBD coronaviruses' RdRp activity, may be promising candidate for disease 2019.

Язык: Английский

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Nanotechnology and COVID-19: Prevention, diagnosis, vaccine, and treatment strategies

Frontiers in Materials, Год журнала: 2023, Номер 9

Опубликована: Янв. 11, 2023

In December 2019, Coronavirus pandemic (COVID-19) caused by Severe Acute Respiratory Syndrome 2 (SARS-CoV-2) viruses, which affected the whole world, is emerged. The details on epidemiology, infection source, transmission mode, and prognosis of SARS-CoV-2 gave in this review. Universal control standards such as hand hygiene, environmental cleanliness, use personal protective equipment, quarantine used to prevent spread COVID-19 without vaccine. However, many vaccine candidate studies carried out globally with using traditional technological approaches. Innovations technology allow development nanotechnological tools formation systems that will inactivate patients. It expected include technologies combine different disciplines, especially robotic applications, antimicrobial nanotechnology, tissue engineering for future treatment COVID-19. This review-based work discusses relationship nanotechnology based working principles.

Язык: Английский

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Silk Fibroin-Coated Nano-MOFs Enhance the Thermal Stability and Immunogenicity of HBsAg

ACS Applied Materials & Interfaces, Год журнала: 2024, Номер 16(7), С. 8346 - 8364

Опубликована: Фев. 7, 2024

Vaccines are widely regarded as one of the most effective weapons in fight against infectious diseases. Currently, vaccines must be stored and transported at low temperatures high can lead to a loss vaccine conformation reduced therapeutic efficacy. Metal–organic frameworks (MOFs), such zeolitic imidazole framework-8 (ZIF-8), new class hybrid materials with large specific surface areas, loading rates, good biocompatibility successful systems for delivery protection. Silk fibroin (SF) has thermal stability. In this study, hepatitis B antigen (HBsAg) was successfully encapsulated ZIF-8 form HBsAg@ZIF-8 (HZ) using one-step shake one-pot method. Subsequently, SF coating modifies HZ through hydrophobic interactions HBsAg/SF@ZIF-8 (HSZ), which enhanced stability immunogenicity HBsAg. Compared free HBsAg, HSZ improved thermostability promoted uptake lysosomal escape, stimulated dendritic cell maturation cytokine secretion, formed an reservoir promote antibody production, activated CD4+ T CD8+ cells enhance memory T-cell production. Importantly, induced strong immune response even after 14 days storage 25 °C. Furthermore, nanoparticles prepared by method exhibited superior properties compared those This study highlights importance SF-coated ZIF-8, holds promise investigating thermostable breaking cold chain.

Язык: Английский

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Nanocarrier vaccines for respiratory infections

Trends in Molecular Medicine, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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