bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2022,
Номер
unknown
Опубликована: Ноя. 28, 2022
Abstract
Tissue-resident
macrophages
are
highly
heterogenous
and
perform
various
dedicated
functions
depending
on
their
locations.
In
particular,
skin
resident
have
intriguing
roles
in
long-distance
intercellular
signaling
by
mediating
cellular
protrusions
called
‘airinemes’
zebrafish.
During
pigment
pattern
formation,
relay
molecules
containing
‘airineme
vesicles’
from
one
cell
to
another.
Without
macrophages,
airineme-mediated
is
abolished,
disrupting
formation.
It
remains
unknown,
however,
if
the
same
macrophage
population
controls
both
these
typical
immune
or
a
separate
subpopulation
communication.
this
study,
with
high-resolution
confocal
live-imaging
type-specific
genetic
ablation
approaches
vivo
,
we
identified
responsible
for
signaling.
These
cells
appear
distinct
conventional
amoeboid
morphology
faster/expansive
migratory
behaviors.
Instead,
show
that
they
resemble
ectoderm-derived
termed
metaphocytes.
Metaphocyte
dramatically
reduces
airineme
extension
addition,
amoeboid/metaphocytes
require
high
levels
of
MMP9
expression
migration
results
reveal
novel
specialized
signaling,
which
may
play
many
other
aspects
Cell,
Год журнала:
2024,
Номер
187(2), С. 276 - 293.e23
Опубликована: Янв. 1, 2024
During
development,
morphogens
pattern
tissues
by
instructing
cell
fate
across
long
distances.
Directly
visualizing
morphogen
transport
in
situ
has
been
inaccessible,
so
the
molecular
mechanisms
ensuring
successful
delivery
remain
unclear.
To
tackle
this
longstanding
problem,
we
developed
a
mouse
model
for
compromised
sonic
hedgehog
(SHH)
and
discovered
that
endocytic
recycling
promotes
SHH
loading
into
signaling
filopodia
called
cytonemes.
We
optimized
methods
to
preserve
vivo
cytonemes
advanced
microscopy
show
endogenous
localized
developing
neural
tubes.
Depletion
of
from
tube
alters
neuronal
fates
compromises
neurodevelopment.
Mutation
filopodial
motor
myosin
10
(MYO10)
reduces
cytoneme
length
density,
which
corrupts
activity
both
WNT.
Combined,
these
results
demonstrate
cytoneme-based
signal
provides
essential
contributions
dispersion
during
mammalian
tissue
development
suggest
MYO10
is
key
regulator
function.
Annual Review of Cell and Developmental Biology,
Год журнала:
2023,
Номер
39(1), С. 307 - 329
Опубликована: Июль 5, 2023
Filopodia
are
dynamic
cell
surface
protrusions
used
for
motility,
pathogen
infection,
and
tissue
development.
The
molecular
mechanisms
determining
how
where
filopodia
grow
retract
need
to
integrate
mechanical
forces
membrane
curvature
with
extracellular
signaling
the
broader
state
of
cytoskeleton.
involved
actin
regulatory
machinery
nucleates,
elongates,
bundles
filaments
separately
from
underlying
cortex.
refined
geometry
filopodia,
importance
context,
high
spatiotemporal
resolution
required,
degree
redundancy
all
limit
current
models.
New
technologies
improving
opportunities
functional
insight,
reconstitution
in
vitro
purified
components,
endogenous
genetic
modification,
inducible
perturbation
systems,
study
multicellular
environments.
In
this
review,
we
explore
recent
advances
conceptual
models
form,
molecules
process,
our
latest
understanding
vivo.
Signal Transduction and Targeted Therapy,
Год журнала:
2024,
Номер
9(1)
Опубликована: Дек. 5, 2024
Abstract
Neutrophils,
the
most
abundant
type
of
granulocyte,
are
widely
recognized
as
one
pivotal
contributors
to
acute
inflammatory
response.
Initially,
neutrophils
were
considered
mobile
infantry
innate
immune
system,
tasked
with
immediate
response
invading
pathogens.
However,
recent
studies
have
demonstrated
that
versatile
cells,
capable
regulating
various
biological
processes
and
impacting
both
human
health
disease.
Cytokines
other
active
mediators
regulate
functional
activity
by
activating
multiple
receptors
on
these
thereby
initiating
downstream
signal
transduction
pathways.
Dysfunctions
in
disruptions
neutrophil
homeostasis
been
implicated
pathogenesis
numerous
diseases,
including
cancer
disorders,
often
due
aberrant
intracellular
signaling.
This
review
provides
a
comprehensive
synthesis
functions,
integrating
advancements
this
field.
Moreover,
it
examines
roles
signaling
pathways
involved
regulation
activity.
The
pathophysiology
diseases
emerging
therapeutic
approaches
targeting
them
also
elaborated.
addresses
current
limitations
within
field
research,
highlighting
critical
gaps
knowledge
warrant
further
investigation.
In
summary,
seeks
establish
multidimensional
model
regulation,
providing
new
perspectives
for
potential
clinical
applications
research.
Cells,
Год журнала:
2023,
Номер
12(2), С. 225 - 225
Опубликована: Янв. 5, 2023
A
morphogen
is
a
signaling
molecule
that
induces
specific
cellular
responses
depending
on
its
local
concentration.
The
concept
of
morphogenic
gradients
has
been
central
paradigm
developmental
biology
for
decades.
Sonic
Hedgehog
(Shh)
one
the
most
important
morphogens
displays
pleiotropic
functions
during
embryonic
development,
ranging
from
neuronal
patterning
to
axon
guidance.
It
commonly
accepted
Shh
distributed
in
gradient
several
tissues
different
origins
development;
however,
how
these
are
formed
and
maintained
at
molecular
levels
still
center
great
deal
research.
In
this
review,
we
first
explored
all
sources
development
nervous
system.
Then,
detailed
can
distribute
surrounding
via
variety
mechanisms.
Finally,
addressed
disrupting
distribution
induce
severe
neurodevelopmental
disorders
cancers.
Although
field
neurodevelopment
since
fifties,
also
describe
contemporary
leading-edge
techniques,
such
as
organoids,
revisit
classical
model.
The Journal of Cell Biology,
Год журнала:
2024,
Номер
223(9)
Опубликована: Июнь 6, 2024
Extracellular
vesicles
(EVs)
are
released
by
many
cell
types,
including
neurons,
carrying
cargoes
involved
in
signaling
and
disease.
It
is
unclear
whether
EVs
promote
intercellular
or
serve
primarily
to
dispose
of
unwanted
materials.
We
show
that
loss
multivesicular
endosome-generating
endosomal
sorting
complex
required
for
transport
(ESCRT)
machinery
disrupts
release
EV
from
Drosophila
motor
neurons.
Surprisingly,
ESCRT
depletion
does
not
affect
the
activities
cargo
Synaptotagmin-4
(Syt4)
only
some
evenness
interrupted
(Evi).
Thus,
these
may
require
transfer
via
EVs,
instead
be
conventionally
secreted
function
cell-autonomously
neuron.
find
phagocytosed
glia
muscles,
disruption
causes
compensatory
autophagy
presynaptic
suggesting
one
several
redundant
mechanisms
remove
synapses.
Our
results
suggest
synaptic
serves
as
a
proteostatic
mechanism
certain
cargoes.
Insects,
Год журнала:
2024,
Номер
15(7), С. 535 - 535
Опубликована: Июль 16, 2024
Eyespot
foci
on
butterfly
wings
function
as
organizers
of
eyespot
color
patterns
during
development.
Despite
their
importance,
focal
structures
have
not
been
examined
in
detail.
Here,
we
microscopically
scales,
sockets,
and
the
wing
membrane
both
expanded
unexpanded
using
Blue
Pansy
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(3), С. 881 - 881
Опубликована: Янв. 21, 2025
Morphogens,
which
are
non-classical
transcription
factors,
according
to
several
studies,
display
a
crucial
role
in
tissue
patterning,
organ
architecture
establishment,
and
human
disease
pathogenesis.
Recent
advances
have
expanded
the
morphogen
participation
wide
range
of
diseases.
There
many
genetic
syndromes
caused
by
mutations
components
signaling
pathways.
The
aberrant
pathways
also
promote
cancer
cell
maintenance,
renewal,
proliferation,
migration.
On
other
hand,
exosomes
their
application
biomedical
field
evolving
significance.
evidence
that
membrane
structures
participate
creation
morphogenic
gradience
biodistribution
renders
them
attractive
as
new
therapeutic
tools.
This
intercellular
transport
is
performed
cell-derived
structures,
mainly
cytonemes,
extracellular
substances
like
heparan
sulphate
proteoglycans
lipoproteins.
interaction
between
morphogens
Extracellular
Vesicles
has
been
observed
at
first
most
studied
insect,
Drosophila,
afterwards
analogous
findings
proved
vertebrates.
review
presents
protagonists
mechanisms
lipid-modified
(Hedgehog
Wnt/β-catenin)
biodistribution.