A macrophage subpopulation promotes airineme-mediated intercellular communication in a Matrix Metalloproteinase-9 dependent manner DOI Creative Commons
Raquel Lynn Bowman, Daoqin Wang, Dae Seok Eom

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2022, Номер unknown

Опубликована: Ноя. 28, 2022

Abstract Tissue-resident macrophages are highly heterogenous and perform various dedicated functions depending on their locations. In particular, skin resident have intriguing roles in long-distance intercellular signaling by mediating cellular protrusions called ‘airinemes’ zebrafish. During pigment pattern formation, relay molecules containing ‘airineme vesicles’ from one cell to another. Without macrophages, airineme-mediated is abolished, disrupting formation. It remains unknown, however, if the same macrophage population controls both these typical immune or a separate subpopulation communication. this study, with high-resolution confocal live-imaging type-specific genetic ablation approaches vivo , we identified responsible for signaling. These cells appear distinct conventional amoeboid morphology faster/expansive migratory behaviors. Instead, show that they resemble ectoderm-derived termed metaphocytes. Metaphocyte dramatically reduces airineme extension addition, amoeboid/metaphocytes require high levels of MMP9 expression migration results reveal novel specialized signaling, which may play many other aspects

Язык: Английский

Cellular and molecular mechanisms of Hedgehog signalling DOI
Yunxiao Zhang, Philip A. Beachy

Nature Reviews Molecular Cell Biology, Год журнала: 2023, Номер 24(9), С. 668 - 687

Опубликована: Март 17, 2023

Язык: Английский

Процитировано

104
Cytoneme signaling provides essential contributions to mammalian tissue patterning DOI Creative Commons
Eric T. Hall, Miriam E. Dillard,

Elizabeth R. Cleverdon

и другие.

Cell, Год журнала: 2024, Номер 187(2), С. 276 - 293.e23

Опубликована: Янв. 1, 2024

During development, morphogens pattern tissues by instructing cell fate across long distances. Directly visualizing morphogen transport in situ has been inaccessible, so the molecular mechanisms ensuring successful delivery remain unclear. To tackle this longstanding problem, we developed a mouse model for compromised sonic hedgehog (SHH) and discovered that endocytic recycling promotes SHH loading into signaling filopodia called cytonemes. We optimized methods to preserve vivo cytonemes advanced microscopy show endogenous localized developing neural tubes. Depletion of from tube alters neuronal fates compromises neurodevelopment. Mutation filopodial motor myosin 10 (MYO10) reduces cytoneme length density, which corrupts activity both WNT. Combined, these results demonstrate cytoneme-based signal provides essential contributions dispersion during mammalian tissue development suggest MYO10 is key regulator function.

Язык: Английский

Процитировано

21
Filopodia In Vitro and In Vivo DOI Creative Commons
Thomas C. A. Blake, Jennifer L. Gallop

Annual Review of Cell and Developmental Biology, Год журнала: 2023, Номер 39(1), С. 307 - 329

Опубликована: Июль 5, 2023

Filopodia are dynamic cell surface protrusions used for motility, pathogen infection, and tissue development. The molecular mechanisms determining how where filopodia grow retract need to integrate mechanical forces membrane curvature with extracellular signaling the broader state of cytoskeleton. involved actin regulatory machinery nucleates, elongates, bundles filaments separately from underlying cortex. refined geometry filopodia, importance context, high spatiotemporal resolution required, degree redundancy all limit current models. New technologies improving opportunities functional insight, reconstitution in vitro purified components, endogenous genetic modification, inducible perturbation systems, study multicellular environments. In this review, we explore recent advances conceptual models form, molecules process, our latest understanding vivo.

Язык: Английский

Процитировано

25
Neutrophil diversity and function in health and disease DOI Creative Commons

F Zhang,

Yidan Xia,

Jiayang Su

и другие.

Signal Transduction and Targeted Therapy, Год журнала: 2024, Номер 9(1)

Опубликована: Дек. 5, 2024

Abstract Neutrophils, the most abundant type of granulocyte, are widely recognized as one pivotal contributors to acute inflammatory response. Initially, neutrophils were considered mobile infantry innate immune system, tasked with immediate response invading pathogens. However, recent studies have demonstrated that versatile cells, capable regulating various biological processes and impacting both human health disease. Cytokines other active mediators regulate functional activity by activating multiple receptors on these thereby initiating downstream signal transduction pathways. Dysfunctions in disruptions neutrophil homeostasis been implicated pathogenesis numerous diseases, including cancer disorders, often due aberrant intracellular signaling. This review provides a comprehensive synthesis functions, integrating advancements this field. Moreover, it examines roles signaling pathways involved regulation activity. The pathophysiology diseases emerging therapeutic approaches targeting them also elaborated. addresses current limitations within field research, highlighting critical gaps knowledge warrant further investigation. In summary, seeks establish multidimensional model regulation, providing new perspectives for potential clinical applications research.

Язык: Английский

Процитировано

11
Butterfly pupal wing tissue with an eyespot organizer DOI Creative Commons
Joji M. Otaki, Atsuko Tanaka,

Euichi Hirose

и другие.

Cells and Development, Год журнала: 2025, Номер unknown, С. 203992 - 203992

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

2
Establishing Hedgehog Gradients during Neural Development DOI Creative Commons
Sara Douceau, Tanya Deutsch Guerrero, Julien Ferent

и другие.

Cells, Год журнала: 2023, Номер 12(2), С. 225 - 225

Опубликована: Янв. 5, 2023

A morphogen is a signaling molecule that induces specific cellular responses depending on its local concentration. The concept of morphogenic gradients has been central paradigm developmental biology for decades. Sonic Hedgehog (Shh) one the most important morphogens displays pleiotropic functions during embryonic development, ranging from neuronal patterning to axon guidance. It commonly accepted Shh distributed in gradient several tissues different origins development; however, how these are formed and maintained at molecular levels still center great deal research. In this review, we first explored all sources development nervous system. Then, detailed can distribute surrounding via variety mechanisms. Finally, addressed disrupting distribution induce severe neurodevelopmental disorders cancers. Although field neurodevelopment since fifties, also describe contemporary leading-edge techniques, such as organoids, revisit classical model.

Язык: Английский

Процитировано

13
ESCRT disruption provides evidence against trans-synaptic signaling via extracellular vesicles DOI Creative Commons
Erica C. Dresselhaus, Kathryn P. Harris, Cassandra R. Blanchette

и другие.

The Journal of Cell Biology, Год журнала: 2024, Номер 223(9)

Опубликована: Июнь 6, 2024

Extracellular vesicles (EVs) are released by many cell types, including neurons, carrying cargoes involved in signaling and disease. It is unclear whether EVs promote intercellular or serve primarily to dispose of unwanted materials. We show that loss multivesicular endosome-generating endosomal sorting complex required for transport (ESCRT) machinery disrupts release EV from Drosophila motor neurons. Surprisingly, ESCRT depletion does not affect the activities cargo Synaptotagmin-4 (Syt4) only some evenness interrupted (Evi). Thus, these may require transfer via EVs, instead be conventionally secreted function cell-autonomously neuron. find phagocytosed glia muscles, disruption causes compensatory autophagy presynaptic suggesting one several redundant mechanisms remove synapses. Our results suggest synaptic serves as a proteostatic mechanism certain cargoes.

Язык: Английский

Процитировано

4
Socket Array Irregularities and Wing Membrane Distortions at the Eyespot Foci of Butterfly Wings Suggest Mechanical Signals for Color Pattern Determination DOI Creative Commons

Yugo Nakazato,

Joji M. Otaki

Insects, Год журнала: 2024, Номер 15(7), С. 535 - 535

Опубликована: Июль 16, 2024

Eyespot foci on butterfly wings function as organizers of eyespot color patterns during development. Despite their importance, focal structures have not been examined in detail. Here, we microscopically scales, sockets, and the wing membrane both expanded unexpanded using Blue Pansy

Язык: Английский

Процитировано

3
The Role of Cytonemes and Diffusive Transport in the Establishment of Morphogen Gradients DOI
Jay A. Stotsky, Hans G. Othmer

Bulletin of Mathematical Biology, Год журнала: 2025, Номер 87(2)

Опубликована: Янв. 3, 2025

Язык: Английский

Процитировано

0
Morphogens and Cell-Derived Structures (Exosomes and Cytonemes) as Components of the Communication Between Cells DOI Open Access

Stavros Chideriotis,

Alkmini T. Anastasiadi, Vassilis L. Tzounakas

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(3), С. 881 - 881

Опубликована: Янв. 21, 2025

Morphogens, which are non-classical transcription factors, according to several studies, display a crucial role in tissue patterning, organ architecture establishment, and human disease pathogenesis. Recent advances have expanded the morphogen participation wide range of diseases. There many genetic syndromes caused by mutations components signaling pathways. The aberrant pathways also promote cancer cell maintenance, renewal, proliferation, migration. On other hand, exosomes their application biomedical field evolving significance. evidence that membrane structures participate creation morphogenic gradience biodistribution renders them attractive as new therapeutic tools. This intercellular transport is performed cell-derived structures, mainly cytonemes, extracellular substances like heparan sulphate proteoglycans lipoproteins. interaction between morphogens Extracellular Vesicles has been observed at first most studied insect, Drosophila, afterwards analogous findings proved vertebrates. review presents protagonists mechanisms lipid-modified (Hedgehog Wnt/β-catenin) biodistribution.

Язык: Английский

Процитировано

0