Doubled haploid technology for line development in maize: technical advances and prospects

Theoretical and Applied Genetics, Год журнала: 2019, Номер 132(12), С. 3227 - 3243

Опубликована: Сен. 25, 2019

Key Message Increased efficiencies achieved in different steps of DH line production offer greater benefits to maize breeding programs. Abstract Doubled haploid (DH) technology has become an integral part many commercial programs as lines several economic, logistic and genetic over conventional inbred lines. Further, new advances continue improve the efficiency development fuel its increased adoption worldwide. The established method for covered this review involves vivo induction maternal haploids by a male inducer genotype, identification from diploids at seed or seedling stage, chromosome doubling ( D 0 ) seedlings finally, selfing fertile plants. Development inducers with high rates adaptation target environments have facilitated tropics. New marker systems identification, such red root oil marker, are being increasingly integrated into potential make accessible germplasm some Flint, landrace, tropical material, where standard R1 - nj is inhibited. Automation holds great promise further reduce cost time identification. Increasing success protocols and/or reducing environmental human toxicity protocols, including research on improvement spontaneous doubling, greatly costs per line.

Язык: Английский

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Heterosis and Hybrid Crop Breeding: A Multidisciplinary Review

Frontiers in Genetics, Год журнала: 2021, Номер 12

Опубликована: Фев. 24, 2021

Although hybrid crop varieties are among the most popular agricultural innovations, rationale for breeding is sometimes misunderstood. Hybrid slower and more resource-intensive than inbred breeding, but it allows systematic improvement of a population by recurrent selection exploitation heterosis simultaneously. Inbred parental lines can identically reproduce both themselves their F

Язык: Английский

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European maize landraces made accessible for plant breeding and genome-based studies

Theoretical and Applied Genetics, Год журнала: 2019, Номер 132(12), С. 3333 - 3345

Опубликована: Сен. 26, 2019

Key message Doubled-haploid libraries from landraces capture native genetic diversity for a multitude of quantitative traits and make it accessible breeding genome-based studies. Abstract Maize comprise large allelic diversity. We created doubled-haploid (DH) three European flint maize characterized them with respect to their molecular diversity, population structure, trait means, variances, correlations. In total, 899 DH lines were evaluated using high-quality genotypic multi-environment phenotypic data up 11 environments. The covered 95% the variation present in 35 an earlier study represent original landrace populations unbiased manner. A comprehensive analysis target plant development at early growth stages as well other important agronomic revealed line per se testcross performance. majority 378 testcrosses outperformed commercial hybrids development. For total biomass yield, we observed yield gap 15% between mean lines. also exhibited undesirable like root lodging tillering, but correlations low or nonsignificant. presented atlas is valuable, publicly available resource studies identify novel evaluate prospects genomic prediction landrace-derived material.

Язык: Английский

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Androgenesis-Based Doubled Haploidy: Past, Present, and Future Perspectives

Frontiers in Plant Science, Год журнала: 2022, Номер 12

Опубликована: Янв. 7, 2022

Androgenesis, which entails cell fate redirection within the microgametophyte, is employed widely for genetic gain in plant breeding programs. Moreover, androgenesis-responsive species provide tractable systems studying cycle regulation, meiotic recombination, and apozygotic embryogenesis cells. Past research on androgenesis has focused protocol development with emphasis temperature pretreatments of donor plants or floral buds, tissue culture optimization because different nutritional requirements than somatic embryogenesis. Protocol new genotypes responsive continues to present day, but slowly. There more focus presently understanding how protocols work order extend them additional species. Transcriptomic epigenetic analyses induced microspores have revealed some cellular molecular responses required associated androgenesis. For example, microRNAs appear regulate early microspore external stimuli; trichostatin-A, a histone deacetylase inhibitor, acts as an additive; ά-phytosulfokine, five amino acid sulfated peptide, promotes Additionally, gene transfer genome editing suggest that future endeavors will likely incorporate greater precision composition used doubled haploid breeding, thus realize impact crop improvement. In this review, we evaluate basic applications androgenesis, explore utility genomics technologies development, considerations overcome genotype specificity morphogenic recalcitrance non-model systems.

Язык: Английский

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Genetic diversity and selection signatures in maize landraces compared across 50 years of in situ and ex situ conservation

Heredity, Год журнала: 2021, Номер 126(6), С. 913 - 928

Опубликована: Март 30, 2021

Abstract Genomics-based, longitudinal comparisons between ex situ and in agrobiodiversity conservation strategies can contribute to a better understanding of their underlying effects. However, landrace designations, ambiguous common names, gaps sampling information complicate the identification matching seed lots. Here we report 50-year comparison genetic diversity set 13 accessions from state Morelos, Mexico, conserved since 1967 retrieved same donor families 2017. We interviewed farmer who donated landraces understand germplasm selection criteria. Samples were genotyped by sequencing, producing 74,739 SNPs. Comparing two sample groups, show that genome-wide was similar. In samples had 3.1% fewer SNPs lower pairwise distances ( F st 0.008–0.113) than 0.031–0.128), but displayed heterozygosity. Despite similarities across samples, could identify several loci under when comparing lots, suggesting ongoing evolution fields. Eight chromosomes 3, 5, 6, 10 showed evidence be related with farmers’ criteria surveyed focus groups interviews at site 2017, including wider kernels larger ear size. Our results have implications for collection resampling threatened landraces.

Язык: Английский

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Genotyping of DNA pools identifies untapped landraces and genomic regions to develop next‐generation varieties

Plant Biotechnology Journal, Год журнала: 2023, Номер 21(6), С. 1123 - 1139

Опубликована: Фев. 6, 2023

Landraces, that is, traditional varieties, have a large diversity is underexploited in modern breeding. A novel DNA pooling strategy was implemented to identify promising landraces and genomic regions enlarge the genetic of varieties. As proof concept, pools from 156 American European maize representing 2340 individuals were genotyped with an SNP array assess their genome-wide diversity. They compared elite cultivars produced across 20th century, represented by 327 inbred lines. Detection selective footprints between different geographic origin identified genes involved environmental adaptation (flowering times, growth) tolerance abiotic biotic stress (drought, cold, salinity). Promising developing two indicators estimate contribution genome lines: (i) modified Roger's distance standardized gene (ii) assignation lines using supervised analysis. It showed most do not closely related only 10 landraces, including famous as Reid's Yellow Dent, Lancaster Surecrop Lacaune, cumulated half total Comparison ancestral directly derived more advanced breeding cycles decrease number contribution. New limited contributions enriched haplotype reference than those high Our approach opens avenue for identification pre-breeding.

Язык: Английский

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Genomic prediction with a maize collaborative panel: identification of genetic resources to enrich elite breeding programs

Theoretical and Applied Genetics, Год журнала: 2019, Номер 133(1), С. 201 - 215

Опубликована: Окт. 8, 2019

Язык: Английский

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Genomic prediction in hybrid breeding: I. Optimizing the training set design

Theoretical and Applied Genetics, Год журнала: 2023, Номер 136(8)

Опубликована: Авг. 1, 2023

Abstract Key message Training sets produced by maximizing the number of parent lines, each involved in one cross, had highest prediction accuracy for H0 hybrids, but lowest H1 and H2 hybrids. Genomic holds great promise hybrid breeding optimum composition training set (TS) as determined parents ( n TS ) crosses per c has received little attention. Our objective was to examine $$r_{a}$$ r a GCA lines used (I1 lines) or not (I0 lines), H0, comprising type I0 × I0, I1 I1, respectively, function . In theory, we developed estimates GBLUPs hybrids: (i) $$\hat{r}_{a}$$ ^ based on expected accuracy, (ii) $$\tilde{r}_{a}$$ ~ SCA effects. simulation part, populations were generated using molecular data from two experimental maize sets. Additive dominance effects QTL borrowed literature simulate six scenarios traits differing proportion τ = 1%, 6%, 22%) variance σ G 2 heritability h 0.4, 0.8). Values closely agreed with For given size N TS, hybrids 1. Conversely, 1 yielded concordant results across all both view these opposite trends, choice selection response types depends resources program.

Язык: Английский

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Conventional and Advance Breeding Approaches for Developing Abiotic Stress Tolerant Maize

Опубликована: Янв. 1, 2024

Conventional breeding approaches in crops continue to deliver improved genotypes farmers since long back; however, the ever-increasing population is a great challenge for researchers produce sufficient food from available land increasing adverse effects of changing climate. In era climate change, several abiotic and biotic stresses are prevalent affecting maize production worldwide. Among stresses, drought, water logging heat important yield restraining factors maize, which adversely its growth development. They affect biomass production, grains formation development by intervening plant physiological, morphological, anatomical biochemical mechanisms. Therefore, it major concern all breeders develop resilient that ensure global security threatened There built mechanisms tolerant help their osmotic adjustment, leaf canopy temperature regulation, stomata conductance, deep root systems development, availability soluble sugar, aerenchyma etc., under various conditions. Breeding stress tolerance includes selection utilization resistant/tolerant germplasm, use molecular markers, secondary traits through different techniques. Wide or distant related germplasm like teosinte provides useful source drought tolerance. addition conventional approaches, recent advances biotechnology have provided powerful tools accelerate gains adaptation. Marker assisted (MAS), genome editing genomic innovative techniques can increase speed up genetic gain any improvement programme. Many Quantitative Trait Loci (QTLs) been mapped water-logging be integrated biology, "omics" has proved potential detail study genes transcriptional play significant roles regulation maize. Biotechnological only utilized as supplement alone they may not able invent survive stresses.

Язык: Английский

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Marker-assisted Recurrent Selection in Plant Breeding

CABI eBooks, Год журнала: 2025, Номер unknown, С. 166 - 193

Опубликована: Фев. 18, 2025

Язык: Английский

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