Catalysts,
Год журнала:
2020,
Номер
10(11), С. 1277 - 1277
Опубликована: Ноя. 3, 2020
Lipases
are
biocatalysts
with
a
significant
potential
to
enable
shift
from
current
pollutant
manufacturing
processes
environmentally
sustainable
approaches.
The
main
reason
of
this
prospect
is
their
catalytic
versatility
as
they
carry
out
several
industrially
relevant
reactions
hydrolysis
fats
in
water/lipid
interface
and
synthesis
solvent-free
or
non-aqueous
media
such
transesterification,
interesterification
esterification.
Because
the
outstanding
traits
Rhizopus
oryzae
lipase
(ROL),
1,3-specificity,
high
enantioselectivity
stability
organic
media,
its
application
energy,
food
pharmaceutical
industrial
sector
has
been
widely
studied.
Significant
advances
have
made
biochemical
characterisation
ROL
particularly
how
activity
affected
by
presence
prosequence.
In
addition,
native
heterologous
production
ROL,
latter
cell
factories
like
Escherichia
coli,
Saccharomyces
cerevisiae
Komagataella
phaffii
(Pichia
pastoris),
thoroughly
described.
Therefore,
review,
we
summarise
knowledge
about
R.
(i)
characteristics,
(ii)
strategies
(iii)
applications.
Chemical Society Reviews,
Год журнала:
2022,
Номер
51(15), С. 6251 - 6290
Опубликована: Янв. 1, 2022
Enzyme
immobilization
has
been
developing
since
the
1960s
and
although
many
industrial
biocatalytic
processes
use
technology
to
improve
enzyme
performance,
still
today
we
are
far
from
full
exploitation
of
field.
One
clear
reason
is
that
evaluate
based
on
only
a
few
experiments
not
always
well-designed.
In
contrast
other
reviews
subject,
here
highlight
pitfalls
using
incorrectly
designed
protocols
explain
why
in
cases
sub-optimal
results
obtained.
We
also
describe
solutions
overcome
these
challenges
come
conclusion
recent
developments
material
science,
bioprocess
engineering
protein
science
continue
open
new
opportunities
for
future.
this
way,
immobilization,
being
mature
discipline,
remains
as
subject
high
interest
where
intense
research
necessary
take
advantage
possibilities.
ACS Catalysis,
Год журнала:
2019,
Номер
10(2), С. 1210 - 1223
Опубликована: Дек. 13, 2019
Enzyme
engineering
plays
a
central
role
in
developing
efficient
biocatalysts
for
biotechnology,
biomedicine,
and
life
sciences.
Apart
from
classical
rational
design
directed
evolution
approaches,
machine
learning
methods
have
been
increasingly
applied
to
find
patterns
data
that
help
predict
protein
structures,
improve
enzyme
stability,
solubility,
function,
substrate
specificity,
guide
design.
In
this
Perspective,
we
analyze
the
state
of
art
databases
used
training
validating
predictors
engineering.
We
discuss
current
limitations
challenges
which
community
is
facing
recent
advancements
experimental
theoretical
potential
address
those
challenges.
also
present
our
view
on
possible
future
directions
applications
biocatalysts.
ACS Central Science,
Год журнала:
2021,
Номер
7(1), С. 55 - 71
Опубликована: Янв. 14, 2021
Biocatalysis,
using
defined
enzymes
for
organic
transformations,
has
become
a
common
tool
in
synthesis,
which
is
also
frequently
applied
industry.
The
generally
high
activity
and
outstanding
stereo-,
regio-,
chemoselectivity
observed
many
biotransformations
are
the
result
of
precise
control
reaction
active
site
biocatalyst.
This
achieved
by
exact
positioning
reagents
relative
to
each
other
fine-tuned
3D
environment,
specific
activating
interactions
between
protein,
subtle
movements
catalyst.
Enzyme
engineering
enables
one
adapt
catalyst
desired
process.
A
well-filled
biocatalytic
toolbox
ready
be
used
various
reactions.
Providing
nonnatural
conditions
evolving
biocatalysts
play
with
myriad
options
creating
novel
transformations
thereby
opening
new,
short
pathways
target
molecules.
Combining
several
pot
perform
reactions
concurrently
increases
efficiency
biocatalysis
even
further.
Chemical Science,
Год журнала:
2020,
Номер
11(10), С. 2587 - 2605
Опубликована: Янв. 1, 2020
Enzymes
are
excellent
catalysts
that
increasingly
being
used
in
industry
and
academia.
This
Perspective
provides
a
general
practical
guide
to
enzymes
their
synthetic
potential,
primarily
aimed
at
organic
chemists.
Reaction Chemistry & Engineering,
Год журнала:
2020,
Номер
5(12), С. 2155 - 2184
Опубликована: Янв. 1, 2020
Very
recent
developments
in
the
field
of
biocatalysis
continuously
operated
systems.
Special
attention
on
future
perspectives
this
key
emerging
technological
area
ranging
from
process
analytical
technologies
to
digitalization.
ACS Catalysis,
Год журнала:
2019,
Номер
9(12), С. 11207 - 11241
Опубликована: Окт. 25, 2019
Pollution,
accidents,
and
misinformation
have
earned
the
pharmaceutical
chemical
industry
a
poor
public
reputation,
despite
their
undisputable
importance
to
society.
Biotechnological
advances
hold
promise
enable
future
of
drastically
reduced
environmental
impact
rigorously
more
efficient
production
routes
at
same
time.
This
is
exemplified
in
Baeyer–Villiger
reaction,
which
offers
simple
synthetic
route
oxidize
ketones
esters,
but
application
hampered
by
requirement
hazardous
dangerous
reagents.
As
an
attractive
alternative,
flavin-containing
monooxygenases
(BVMOs)
been
investigated
for
potential
as
biocatalysts
long
time,
many
variants
characterized.
After
general
look
state
biotechnology,
we
here
summarize
literature
on
biochemical
characterizations,
mechanistic
structural
investigations,
well
enzyme
engineering
efforts
BVMOs.
With
focus
recent
developments,
critically
outline
toward
tuning
these
enzymes
suitable
industrial
applications.
Biotechnology Advances,
Год журнала:
2020,
Номер
51, С. 107615 - 107615
Опубликована: Авг. 19, 2020
Peroxygenases
are
an
emerging
new
class
of
enzymes
allowing
selective
oxyfunctionalisation
reactions
in
a
cofactor-independent
way
different
from
well-known
P450
monooxygenases.
Herein,
we
focused
on
recent
developments
organic
synthesis,
molecular
biotechnology
and
reaction
engineering
viewpoints
that
devoted
to
bring
these
industrial
applications.
This
covers
natural
diversity
sources,
protein
strategies
for
expression,
substrate
scope,
activity
selectivity,
stabilisation
via
immobilisation,
the
use
peroxygenases
low
water
media.
We
believe
have
much
offer
oxyfunctionalisations
study
explore
full
potential
versatile
biocatalysts
synthesis.
Energy & Fuels,
Год журнала:
2024,
Номер
38(3), С. 1593 - 1617
Опубликована: Янв. 16, 2024
This
review
illuminates
the
pivotal
synergy
between
machine
learning
(ML)
and
biopolymers,
spotlighting
their
combined
potential
to
reshape
sustainable
energy,
fuels,
biochemicals.
Biobased
polymers,
derived
from
renewable
sources,
have
garnered
attention
for
roles
in
energy
fuel
sectors.
These
when
integrated
with
ML
techniques,
exhibit
enhanced
functionalities,
optimizing
systems,
storage,
conversion.
Detailed
case
studies
reveal
of
biobased
polymers
applications
industry,
further
showcasing
how
bolsters
efficiency
innovation.
The
intersection
also
marks
advancements
biochemical
production,
emphasizing
innovations
drug
delivery
medical
device
development.
underscores
imperative
harnessing
convergence
future
global
sustainability
endeavors
collective
evidence
presented
asserts
immense
promise
this
union
holds
steering
a
innovative
trajectory.
