Lung regeneration: diverse cell types and the therapeutic potential

MedComm, Год журнала: 2024, Номер 5(2)

Опубликована: Фев. 1, 2024

Abstract Lung tissue has a certain regenerative ability and triggers repair procedures after injury. Under controllable conditions, lung can restore normal structure function. Disruptions in this process lead to respiratory system failure even death, causing substantial medical burden. The main types of diseases are chronic obstructive pulmonary disease (COPD), idiopathic fibrosis (IPF), acute distress syndrome (ARDS). Multiple cells, such as epithelial endothelial fibroblasts, immune involved regulating the Although mechanism that regulates not been fully elucidated, clinical trials targeting different cells signaling pathways have achieved some therapeutic effects diseases. In review, we provide an overview cell type regeneration repair, research models, summarize molecular mechanisms regulation fibrosis. Moreover, discuss current stem therapy pharmacological strategies for COPD, IPF, ARDS treatment. This review provides reference further on cellular regeneration, drug development, trials.

Язык: Английский

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Lung Organoids—The Ultimate Tool to Dissect Pulmonary Diseases?

Frontiers in Cell and Developmental Biology, Год журнала: 2022, Номер 10

Опубликована: Июль 13, 2022

Organoids are complex multicellular three-dimensional (3D) in vitro models that designed to allow accurate studies of the molecular processes and pathologies human organs. can be derived from a variety cell types, such as primary progenitor cells, pluripotent stem or tumor-derived cells co-cultured with immune microbial further mimic tissue niche. Here, we focus on development 3D lung organoids their use disease drug screening tools. We introduce various experimental approaches used model diseases analyze advantages disadvantages. also discuss validation physiological relevance study diseases. Furthermore, summarize current host-pathogen interactions cystic fibrosis, chronic obstructive pulmonary disease, SARS-CoV-2 infection. Moreover, tumor cancer application personalized medicine research. Finally, outline future research field induced cell-derived organoids.

Язык: Английский

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Cryobiopsy: A Breakthrough Strategy for Clinical Utilization of Lung Cancer Organoids

Cells, Год журнала: 2023, Номер 12(14), С. 1854 - 1854

Опубликована: Июль 14, 2023

One major challenge associated with lung cancer organoids (LCOs) is their predominant derivation from surgical specimens of patients early-stage cancer. However, advanced cancer, who are in need chemotherapy, often cannot undergo surgery. Therefore, there an urgent to successfully generate LCOs biopsy specimens. Conventional techniques, such as transthoracic needle and forceps biopsy, only yield small amounts tissue, resulting a low success rate for culturing samples. Furthermore, potential complications, like bleeding pneumothorax, make it difficult obtain sufficient tissue. Another critical issue the overgrowth normal cells later passages LCO culture, optimal culture conditions yet be determined. To address these limitations, we attempted create cryobiopsy obtained (n = 113). Overall, initial establishing samples was 40.7% 46). Transbronchial enables retrieval significantly larger tissue than bronchoscopic biopsy. Additionally, can employed peripheral lesions, aided via radial endobronchial ultrasonography. This study improved demonstrated that retained characteristics resembled primary tumors. Single-cell RNA sequencing confirmed high cell purity early derived three-dimensional structure intracellular components were characterized using holotomography. Finally, drug screening performed specialized micropillar system cryobiopsy-derived LCOs. offer promising solution limitations conventional Cryobiopsy applied at all stages, including those provide generation. anticipate will serve breakthrough strategy clinical application stages

Язык: Английский

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Organoid models: assessing lung cell fate decisions and disease responses

Trends in Molecular Medicine, Год журнала: 2021, Номер 27(12), С. 1159 - 1174

Опубликована: Окт. 19, 2021

Язык: Английский

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Inhibitors of the Elastase LasB for the Treatment of Pseudomonas aeruginosa Lung Infections

ACS Central Science, Год журнала: 2023, Номер 9(12), С. 2205 - 2215

Опубликована: Окт. 27, 2023

Infections caused by the Gram-negative pathogen Pseudomonas aeruginosa are emerging worldwide as a major threat to human health. Conventional antibiotic monotherapy suffers from rapid resistance development, underlining urgent need for novel treatment concepts. Here, we report on nontraditional approach combat P. aeruginosa-derived infections targeting its main virulence factor, elastase LasB. We discovered new chemical class of phosphonates with an outstanding in vitro ADMET and PK profile, auspicious activity both vivo. established mode action through cocrystal structure our lead compound LasB several ex vivo models. The proof concept combination pathoblocker levofloxacin murine neutropenic lung infection model reduction protein levels blood target engagement demonstrate great potential use adjunctive humans.

Язык: Английский

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Lung Organoids: Systematic Review of Recent Advancements and its Future Perspectives

Tissue Engineering and Regenerative Medicine, Год журнала: 2024, Номер 21(5), С. 653 - 671

Опубликована: Март 11, 2024

Язык: Английский

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Reconstructing the pulmonary niche with stem cells: a lung story

Stem Cell Research & Therapy, Год журнала: 2022, Номер 13(1)

Опубликована: Апрель 11, 2022

The global burden of pulmonary disease highlights an overwhelming need in improving our understanding lung development, disease, and treatment. It also calls for further advances ability to engineer the system at cellular tissue levels. discovery human pluripotent stem cells (hPSCs) offsets relative inaccessibility lungs studying developmental programs mechanisms, all while offering a potential source regenerative interventions. This review offers perspective on where cell field stands terms accomplishing these ambitious goals. We will trace known stages pathways involved vivo development how they inspire directed differentiation progenitor vitro. recap efforts made date recapitulate niche vitro via engineered cell-cell cell-extracellular matrix (ECM) interactions.

Язык: Английский

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Dynamic 3D in vitro lung models: applications of inorganic nanoparticles for model development and characterization

Nanoscale, Год журнала: 2024, Номер 16(23), С. 10880 - 10900

Опубликована: Янв. 1, 2024

Being a vital organ exposed to the external environment, lung is susceptible plethora of pathogens and pollutants. This reflected in high incidences chronic respiratory diseases, which remain leading cause mortality world-wide pose persistent global burden. It thus paramount importance improve our understanding these pathologies provide better therapeutic options. necessitates development representative physiologically relevant vitro models. Advances bioengineering have enabled sophisticated models that not only capture three-dimensional architecture cellular environment but also incorporate dynamics local biophysical stimuli. However, such complex require novel approaches reliable characterization. Within this review we explore how 3D bioprinting nanoparticles can serve as multifaceted tools develop dynamic 4D printed facilitate their characterization context pulmonary fibrosis breast cancer metastasis.

Язык: Английский

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The Matrix of Mitochondrial Imaging: Exploring Spatial Dimensions

Biomolecules, Год журнала: 2025, Номер 15(2), С. 229 - 229

Опубликована: Фев. 5, 2025

Mitochondria play a crucial role in human biology, affecting cellular processes at the smallest spatial scale as well those involved functionality of whole system. Imaging is most important research tool for studying fundamental mitochondria across these diverse scales. A wide array available imaging techniques have enabled us to visualize mitochondrial structure and behavior, their effect on cells tissues range from micrometers centimeters. Each various that are offers unique advantages tailored specific needs. Selecting an appropriate technique suitable application interest therefore crucial, but can be challenging due large possibilities. The aim this review two-fold. First, we provide overview discuss strengths limitations applications sub-mitochondrial, cellular, tissue organ levels mitochondria. Second, identify opportunities novel advancement field. We emphasize importance integration scales studies, particularly bridge gap between microscopic non-invasive techniques. While integrating challenging, primarily because such multi-scale approaches require expertise spans different modalities, argue has potential groundbreaking insights into biology. By providing comprehensive techniques, paves way initiatives research.

Язык: Английский

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Animal models of chronic obstructive pulmonary disease and their role in drug discovery and development: a critical review

Expert Opinion on Drug Discovery, Год журнала: 2025, Номер unknown

Опубликована: Фев. 12, 2025

Introduction To understand the mechanisms of COPD and to discover test new drugs, laboratory animals are essential. However, complex changes associated with disease in humans difficult fully replicate animal models.

Язык: Английский

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