Recent technological advances in lab-on-a-chip for bone remodeling DOI Creative Commons
Seoyeon Kim, Arun Rajendran, Sivashanmugam Amirthalingam

et al.

Biosensors and Bioelectronics X, Journal Year: 2023, Volume and Issue: 14, P. 100360 - 100360

Published: June 6, 2023

Lab-on-a-chip (LOC) is a three-dimensional cell culture platform with microfluidic flow that biomimics the physiological system of various organs. LOC emerged as future replacement technology for in vitro cell-based models and vivo animal models, gaining popularity field physiology, pharmacology, biology. Nowadays, sensors are incorporated into to monitor real-time parameters its microenvironment response biophysical and/or biochemical stimuli maturation. A considerable amount studies has been conducted detect mechanical, physical, electrical, metabolic engineered This paper aims provide comprehensive insight how biosensor-implemented can be utilized analysis tissue recapitulating systems focus on bone remodeling. We describe chip design, basic principles sensors, ongoing recent efforts perform in-line organoids. review also presents imaging techniques used analytical tools visualizing LOC.

Language: Английский

Lung regeneration: diverse cell types and the therapeutic potential DOI Creative Commons
Yutian Chen, Zhen Li, Gaili Ji

et al.

MedComm, Journal Year: 2024, Volume and Issue: 5(2)

Published: Feb. 1, 2024

Abstract Lung tissue has a certain regenerative ability and triggers repair procedures after injury. Under controllable conditions, lung can restore normal structure function. Disruptions in this process lead to respiratory system failure even death, causing substantial medical burden. The main types of diseases are chronic obstructive pulmonary disease (COPD), idiopathic fibrosis (IPF), acute distress syndrome (ARDS). Multiple cells, such as epithelial endothelial fibroblasts, immune involved regulating the Although mechanism that regulates not been fully elucidated, clinical trials targeting different cells signaling pathways have achieved some therapeutic effects diseases. In review, we provide an overview cell type regeneration repair, research models, summarize molecular mechanisms regulation fibrosis. Moreover, discuss current stem therapy pharmacological strategies for COPD, IPF, ARDS treatment. This review provides reference further on cellular regeneration, drug development, trials.

Language: Английский

Citations

11
Lung Organoids—The Ultimate Tool to Dissect Pulmonary Diseases? DOI Creative Commons
Veronika Bosáková, Marco De Zuani,

Lucie Sládková

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2022, Volume and Issue: 10

Published: July 13, 2022

Organoids are complex multicellular three-dimensional (3D) in vitro models that designed to allow accurate studies of the molecular processes and pathologies human organs. can be derived from a variety cell types, such as primary progenitor cells, pluripotent stem or tumor-derived cells co-cultured with immune microbial further mimic tissue niche. Here, we focus on development 3D lung organoids their use disease drug screening tools. We introduce various experimental approaches used model diseases analyze advantages disadvantages. also discuss validation physiological relevance study diseases. Furthermore, summarize current host-pathogen interactions cystic fibrosis, chronic obstructive pulmonary disease, SARS-CoV-2 infection. Moreover, tumor cancer application personalized medicine research. Finally, outline future research field induced cell-derived organoids.

Language: Английский

Citations

30
Cryobiopsy: A Breakthrough Strategy for Clinical Utilization of Lung Cancer Organoids DOI Creative Commons
Dongil Park, Dahye Lee, Yoonjoo Kim

et al.

Cells, Journal Year: 2023, Volume and Issue: 12(14), P. 1854 - 1854

Published: July 14, 2023

One major challenge associated with lung cancer organoids (LCOs) is their predominant derivation from surgical specimens of patients early-stage cancer. However, advanced cancer, who are in need chemotherapy, often cannot undergo surgery. Therefore, there an urgent to successfully generate LCOs biopsy specimens. Conventional techniques, such as transthoracic needle and forceps biopsy, only yield small amounts tissue, resulting a low success rate for culturing samples. Furthermore, potential complications, like bleeding pneumothorax, make it difficult obtain sufficient tissue. Another critical issue the overgrowth normal cells later passages LCO culture, optimal culture conditions yet be determined. To address these limitations, we attempted create cryobiopsy obtained (n = 113). Overall, initial establishing samples was 40.7% 46). Transbronchial enables retrieval significantly larger tissue than bronchoscopic biopsy. Additionally, can employed peripheral lesions, aided via radial endobronchial ultrasonography. This study improved demonstrated that retained characteristics resembled primary tumors. Single-cell RNA sequencing confirmed high cell purity early derived three-dimensional structure intracellular components were characterized using holotomography. Finally, drug screening performed specialized micropillar system cryobiopsy-derived LCOs. offer promising solution limitations conventional Cryobiopsy applied at all stages, including those provide generation. anticipate will serve breakthrough strategy clinical application stages

Language: Английский

Citations

18
Organoid models: assessing lung cell fate decisions and disease responses DOI
Derek C. Liberti, Edward E. Morrisey

Trends in Molecular Medicine, Journal Year: 2021, Volume and Issue: 27(12), P. 1159 - 1174

Published: Oct. 19, 2021

Language: Английский

Citations

40
Lung Organoids: Systematic Review of Recent Advancements and its Future Perspectives DOI

T. Thangam,

Krupakar Parthasarathy,

Kolli Venkata Supraja

et al.

Tissue Engineering and Regenerative Medicine, Journal Year: 2024, Volume and Issue: 21(5), P. 653 - 671

Published: March 11, 2024

Language: Английский

Citations

4
The Matrix of Mitochondrial Imaging: Exploring Spatial Dimensions DOI Creative Commons
Irene M. G. M. Hemel, Ilja C.W. Arts, Michelle Moerel

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(2), P. 229 - 229

Published: Feb. 5, 2025

Mitochondria play a crucial role in human biology, affecting cellular processes at the smallest spatial scale as well those involved functionality of whole system. Imaging is most important research tool for studying fundamental mitochondria across these diverse scales. A wide array available imaging techniques have enabled us to visualize mitochondrial structure and behavior, their effect on cells tissues range from micrometers centimeters. Each various that are offers unique advantages tailored specific needs. Selecting an appropriate technique suitable application interest therefore crucial, but can be challenging due large possibilities. The aim this review two-fold. First, we provide overview discuss strengths limitations applications sub-mitochondrial, cellular, tissue organ levels mitochondria. Second, identify opportunities novel advancement field. We emphasize importance integration scales studies, particularly bridge gap between microscopic non-invasive techniques. While integrating challenging, primarily because such multi-scale approaches require expertise spans different modalities, argue has potential groundbreaking insights into biology. By providing comprehensive techniques, paves way initiatives research.

Language: Английский

Citations

0
Animal models of chronic obstructive pulmonary disease and their role in drug discovery and development: a critical review DOI
Maria Gabriella Matera, Luigino Calzetta, Barbara Rinaldi

et al.

Expert Opinion on Drug Discovery, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 12, 2025

Introduction To understand the mechanisms of COPD and to discover test new drugs, laboratory animals are essential. However, complex changes associated with disease in humans difficult fully replicate animal models.

Language: Английский

Citations

0
Organoid: Biomedical application, biobanking, and pathways to translation DOI Creative Commons

Aprajita Sinha,

Maheshree Maibam,

Ronit Jain

et al.

Heliyon, Journal Year: 2025, Volume and Issue: unknown, P. e43028 - e43028

Published: Feb. 1, 2025

Language: Английский

Citations

0
Tissue-Informed Biomaterial Innovations Advance Pulmonary Regenerative Engineering DOI

Anton D. Kary,

Haley Noelle,

Chelsea M. Magin

et al.

ACS Macro Letters, Journal Year: 2025, Volume and Issue: unknown, P. 434 - 447

Published: March 18, 2025

Irreversible progressive pulmonary diseases drastically reduce the patient quality of life, while transplantation remains only definitive cure. Research into lung regeneration pathways holds significant potential to expand and promote discovery new treatment options. Polymeric biomaterials designed replicate key tissue characteristics (i.e., biochemical composition mechanical cues) show promise for creating environments in which study chronic initiate regeneration. In this Viewpoint, we explore how naturally derived materials can be employed alone or combined with engineered polymer systems create advanced culture platforms. Pulmonary models have historically leveraged natural materials, including basement membrane extracts a decellularized extracellular matrix, as platforms studies. Here, provide an overview progression regenerative engineering, exploring innovations growing field tissue-informed advance research by bridging gap between biological relevance precision.

Language: Английский

Citations

0
Reconstructing the pulmonary niche with stem cells: a lung story DOI Creative Commons

Barbie Varghese,

Zihan Ling, Xi Ren

et al.

Stem Cell Research & Therapy, Journal Year: 2022, Volume and Issue: 13(1)

Published: April 11, 2022

The global burden of pulmonary disease highlights an overwhelming need in improving our understanding lung development, disease, and treatment. It also calls for further advances ability to engineer the system at cellular tissue levels. discovery human pluripotent stem cells (hPSCs) offsets relative inaccessibility lungs studying developmental programs mechanisms, all while offering a potential source regenerative interventions. This review offers perspective on where cell field stands terms accomplishing these ambitious goals. We will trace known stages pathways involved vivo development how they inspire directed differentiation progenitor vitro. recap efforts made date recapitulate niche vitro via engineered cell-cell cell-extracellular matrix (ECM) interactions.

Language: Английский

Citations

15