JACS Au,
Год журнала:
2024,
Номер
4(4), С. 1664 - 1672
Опубликована: Апрель 9, 2024
The
accurate
and
timely
detection
of
disease
biomarkers
at
the
point-of-care
is
essential
to
ensuring
effective
treatment
epidemiological
surveillance.
Here,
we
report
selection
engineering
RNA-cleaving
DNAzymes
that
respond
specific
genetic
markers
amplify
signals.
Because
target-specific
activation
gene-specific
(gDz)
like
trans-cleavage
activity
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)
CRISPR-associated
(Cas)
machinery,
further
developed
a
CRISPR-like
assay
using
DNAzyme
coupled
with
isothermal
sequence
signal
amplification
(CLARISSA)
for
nucleic
acid
in
clinical
samples.
Building
on
high
specificity
orthogonality
gDzs,
CLARISSA
highly
versatile
expandable
multiplex
testing.
Upon
integration
an
recombinase
polymerase
amplification,
enabled
human
papillomavirus
(HPV)
16
189
cervical
samples
collected
from
cancer
screening
participants
(n
=
189)
100%
sensitivity
97.4%
specificity,
respectively.
A
multiplexed
allowed
simultaneous
analyses
HPV16
HPV18
46
samples,
which
returned
96.3%
83.3%
HPV18,
No
false
positives
were
found
throughout
our
tests.
Besides
fluorescence
readout
fluorogenic
reporter
probes,
also
demonstrated
be
fully
compatible
visual
lateral
flow
readout.
sensitivity,
accessibility,
multiplexity,
believe
ideal
CRISPR-Dx
alternative
diagnosis
field-based
applications.
Analytical Chemistry,
Год журнала:
2021,
Номер
93(7), С. 3325 - 3330
Опубликована: Фев. 11, 2021
Rapid
tests
for
pathogen
identification
and
spread
assessment
are
critical
infectious
disease
control
prevention.
The
of
viral
outbreaks
requires
a
nucleic
acid
diagnostic
test
that
is
sensitive
simple
delivers
fast
reliable
results.
Here,
we
report
one-pot
direct
reverse
transcript
loop-mediated
isothermal
amplification
(RT-LAMP)
assay
SARS-CoV-2
based
on
lateral
flow
in
clinical
samples.
entire
contiguous
sample-to-answer
workflow
takes
less
than
40
min
from
swab
sample
to
result
without
professional
instruments
technicians.
achieved
an
accuracy
100%
12
synthetic
samples
compared
the
data
PCR-based
assays.
We
anticipate
our
method
will
provide
universal
platform
rapid
point-of-care
detection
emerging
diseases.
Abstract
The
physicochemical
nature
of
DNA
allows
the
assembly
highly
predictable
structures
via
several
fabrication
strategies,
which
have
been
applied
to
make
breakthroughs
in
various
fields.
Moreover,
nanostructures
are
regarded
as
materials
with
excellent
editability
and
biocompatibility
for
biomedical
applications.
ongoing
maintenance
release
new
structure
design
tools
ease
work
large
arbitrary
feasible
different
However,
endows
them
stimulus-responsive
mechanisms
capable
responding
biomolecules,
such
nucleic
acids
proteins,
well
biophysical
environmental
parameters,
temperature
pH.
Via
these
mechanisms,
dynamic
settings,
including
basic
research,
active
drug
delivery,
biosensor
development,
tissue
engineering.
These
applications
shown
versatility
nanostructures,
unignorable
merits
that
exceed
those
their
traditional
counterparts,
polymers
metal
particles.
there
stability,
yield,
exogenous
DNA,
ethical
considerations
regarding
clinical
translation.
In
this
review,
we
first
introduce
recent
efforts
discoveries
nanotechnology,
highlighting
uses
Then,
presented,
typical
applications,
use
aptamers,
ion
concentration/pH-sensitive
molecules,
strand
displacement
reactions,
protein-based
discussed.
Finally,
challenges
Journal of the American Chemical Society,
Год журнала:
2023,
Номер
145(13), С. 7540 - 7547
Опубликована: Март 22, 2023
The
classical
DNA
aptamer
for
adenosine
and
ATP
has
been
the
most
used
small
molecule
binding
biosensing,
imaging,
nanotechnology.
This
sequence
recurred
multiple
times
in
previous
selections,
all
selections
a
high
concentration
of
as
target.
Herein,
two
separate
were
performed
using
targets.
By
pushing
target
concentrations
down
to
low
micromolar
range,
new
aptamers
with
Kd
230
nM
obtained,
showing
around
30-fold
higher
affinity
compared
aptamer.
still
dominated
library
early
rounds
but
it
was
suppressed
later
rounds.
bind
one
instead
two.
Mutation
studies
confirmed
their
secondary
structures
specific
binding.
Using
deep
sequencing
data
from
long-standing
questions
such
existence
one-site
mutation
distribution
addressed.
Comparisons
made
previously
reported
ATP.
Finally,
strand-displacement
biosensor
tested
selectivity
its
nucleotides.
Proceedings of the National Academy of Sciences,
Год журнала:
2023,
Номер
120(28)
Опубликована: Июль 3, 2023
Exosomes
(EXOs)
have
been
proven
as
biomarkers
for
disease
diagnosis
and
agents
therapeutics.
Great
challenge
remains
in
the
separation
of
EXOs
with
high-purity
low-damage
from
complex
biological
media,
which
is
critical
downstream
applications.
Herein,
we
report
a
DNA-based
hydrogel
to
realize
specific
nondestructive
media.
The
separated
were
directly
utilized
detection
human
breast
cancer
clinical
samples,
well
applied
therapeutics
myocardial
infarction
rat
models.
materials
chemistry
basis
this
strategy
involved
synthesis
ultralong
DNA
chains
via
an
enzymatic
amplification,
formation
hydrogels
through
complementary
base-pairing.
These
that
contained
polyvalent
aptamers
able
recognize
bind
receptors
on
EXOs,
efficient
binding
ensured
selective
media
into
further
formed
networked
hydrogel.
Based
hydrogel,
rationally
designed
optical
modules
introduced
exosomal
pathogenic
microRNA,
achieved
classification
patients
versus
healthy
donors
100%
precision.
Furthermore,
mesenchymal
stem
cell–derived
was
proved
significant
therapeutic
efficacy
repairing
infarcted
myocardium
We
envision
hydrogel–based
bioseparation
system
promising
powerful
biotechnology,
will
promote
development
extracellular
vesicles
nanobiomedicine.
Lab on a Chip,
Год журнала:
2023,
Номер
23(5), С. 1169 - 1191
Опубликована: Янв. 1, 2023
Digital
microfluidic
(DMF)
bioassays
with
the
benefits
of
automation,
addressability,
integration
and
dynamic
configuration
ability
for
nucleic
acids,
proteins,
immunoreaction
cell
analysis
are
presented
in
this
review.
Applied Sciences,
Год журнала:
2024,
Номер
14(4), С. 1383 - 1383
Опубликована: Фев. 8, 2024
Nowadays,
drug
delivery
has
an
important
role
in
medical
therapy.
The
use
of
biopolymers
developing
systems
(DDSs)
is
increasingly
attracting
attention
due
to
their
remarkable
and
numerous
advantages,
contrast
conventional
polymers.
Biopolymers
have
many
advantages
(biodegradability,
biocompatibility,
renewability,
affordability,
availability),
which
are
extremely
for
materials
with
applications
the
biomedical
field.
Additionally,
appropriate
when
they
improve
functioning
a
number
positive
effects
on
human
life.
Therefore,
this
review
presents
most
used
applications,
especially
delivery.
In
addition,
by
combining
different
DDSs
tailored
functional
properties
(e.g.,
physical
properties,
biodegradability)
can
be
developed.
This
summarizes
provides
data
progress
research
(chitosan,
alginate,
starch,
cellulose,
albumin,
silk
fibroin,
collagen,
gelatin)
DDSs,
preparation,
mechanism
action.
Centrifugal
microfluidics,
with
its
advantages
of
rapid
and
precise
fluid
control
without
the
need
for
external
pressure,
is
widely
applied
in
point-of-care
testing.
Analytical Chemistry,
Год журнала:
2021,
Номер
93(45), С. 15216 - 15223
Опубликована: Ноя. 5, 2021
The
development
of
a
sensitive,
facile,
and
cost-effective
colorimetric
method
is
great
significance
for
the
point-of-care
testing
viral
nucleic
acid.
Herein,
we
reported
strand
displacement
amplification
assisted
CRISPR-Cas12a
(SDACC)
analysis
hepatitis
B
virus
(HBV)
DNA
was
chosen
as
target
to
trigger
(SDA)
generate
abundant
single-strand
(ssDNA)
products.
ssDNA
amplicon
hybridized
with
template
activate
trans-cleavage
activity
CRISPR-Cas12a,
leading
nonspecific
cleavage
on
GOx-ssDNA-modified
magnetic
beads
release
GOx.
released
GOx
capable
catalyzing
substrate
solution
color
change,
which
could
be
directly
observed
by
naked
eyes.
SDACC
strategy
identify
single-base
mismatch
located
in
sequence
achieve
sensitive
detection
HBV
limit
low
41.8
fM.
Notably,
sophisticated
primer
design
complicated
process
circumvented.
current
approach
realizes
simple,
low-cost,
acid
holds
promise
practical
application
infection
diagnosis.
Chemical Society Reviews,
Год журнала:
2021,
Номер
50(9), С. 5650 - 5667
Опубликована: Янв. 1, 2021
DNA
nanotechnology
has
led
to
the
rise
of
nanostructures,
which
possess
programmable
shapes
and
are
capable
organizing
different
functional
molecules
materials.
A
variety
nanostructure-based
imaging
probes
have
been
developed.
