Pathogenic and therapeutic role of exosomes in neurodegenerative disorders

Neural Regeneration Research, Год журнала: 2023, Номер 19(1), С. 75 - 79

Опубликована: Май 25, 2023

Neurodegenerative disorders affect millions of people worldwide, and the prevalence these is only projected to rise as number over 65 will drastically increase in coming years. While therapies exist aid symptomatic relief, effective treatments that can stop or reverse progress each neurodegenerative disease are lacking. Recently, research on role extracellular vesicles markers therapeutics has been intensively studied. Exosomes, 30-150 nm diameter, one type facilitating cell-to-cell communication. Exosomes thought play a propagation variety diseases, such Alzheimer's disease, Parkinson's amyotrophic lateral sclerosis. Accordingly, exosomes derived from patients an invaluable source biomarkers. On other hand, exosomes, especially those stem cells, could serve therapeutic for disorders, seen by rapid clinical trials investigating efficacy different neurological diseases. This review summarizes pathological burden approach disorders. We also highlight how heat shock increases yield while still maintaining their efficacy. Finally, this concludes with outstanding questions remain be addressed exosomal research.

Язык: Английский

---

Optimization of exosome-based cell-free strategies to enhance endogenous cell functions in tissue regeneration

Acta Biomaterialia, Год журнала: 2023, Номер 171, С. 68 - 84

Опубликована: Сен. 19, 2023

Язык: Английский

---

A non-invasive strategy for suppressing asthmatic airway inflammation and remodeling: Inhalation of nebulized hypoxic hUCMSC-derived extracellular vesicles

Frontiers in Immunology, Год журнала: 2023, Номер 14

Опубликована: Апрель 5, 2023

Mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) are extremely promising nanoscale cell-free therapeutic agents. We previously identified that intravenous administration (IV) of human umbilical cord MSC-EVs (hUCMSC-EVs), especially hypoxic hUCMSC-EVs (Hypo-EVs), could suppress allergic airway inflammation and remodeling. Here, we further investigated the effects Hypo-EVs by atomizing inhalation (INH), which is a non-invasive efficient drug delivery method for lung diseases. found nebulized produced atomization system (medical/household air compressor nebulizer) maintained excellent structural integrity. Nebulized Dir-labeled inhaled mice were mainly restricted to lungs. INH significantly reduced inflammatory infiltration, decreased levels IL-4, IL-5 IL-13 in bronchoalveolar lavage fluid (BALF), declined content OVA-specific IgE serum, attenuated goblet cell metaplasia, expressions subepithelial collagen-1 α-smooth muscle actin (α-SMA). Notably, Hypo-EV was generally more potent than IV suppressing α-SMA expressions. RNA sequencing revealed various biological processes, such as adhesion, innate immune response, B activation, space, associated with activity against asthma mice. In addition, load exogenous miR-146a-5p (miR-146a-5p-EVs). Furthermore, miR-146a-5p-EVs resulted increased expression mostly lungs, offered greater protection OVA-induced increase inflammation, collagen accumulation myofibroblast compared Hypo-EVs. Overall, effectively remodeling, potentially creating route use treatment.

Язык: Английский

---

Mesenchymal stem cell-derived extracellular vesicles subvert Th17 cells by destabilizing RORγt through posttranslational modification

Experimental & Molecular Medicine, Год журнала: 2023, Номер 55(3), С. 665 - 679

Опубликована: Март 24, 2023

Mesenchymal stem cell (MSC)-derived small extracellular vesicles (MSC-sEVs) are known to exert immunosuppressive functions. This study showed that MSC-sEVs specifically convert T helper 17 (Th17) cells into IL-17 low-producer (ex-Th17) by degrading RAR-related orphan receptor γt (RORγt) at the protein level. In experimental autoimmune encephalomyelitis (EAE)-induced mice, treatment with was found not only ameliorate clinical symptoms but also reduce number of Th17 in draining lymph nodes and central nervous system. were destabilize RORγt K63 deubiquitination deacetylation, which attributed EP300-interacting inhibitor differentiation 3 (Eid3) contained MSC-sEVs. Small isolated from Eid3 knockdown MSCs Eid3-shRNA failed downregulate RORγt. Moreover, forced expression gene transfection significantly decrease level cells. Altogether, this reveals novel mechanisms MSC-sEVs, suggests feasibility as an attractive therapeutic tool for curing Th17-mediated inflammatory diseases.

Язык: Английский

---

Human umbilical cord blood-mesenchymal stem cell derived exosomes as an efficient nanocarrier for Docetaxel and miR-125a: Formulation optimization and anti-metastatic behaviour

Life Sciences, Год журнала: 2023, Номер 322, С. 121621 - 121621

Опубликована: Март 30, 2023

Язык: Английский

---