Cytotechnology, Год журнала: 2024, Номер 76(4), С. 363 - 401
Опубликована: Май 8, 2024
Язык: Английский
Nature Reviews Drug Discovery, Год журнала: 2021, Номер 20(8), С. 629 - 651
Опубликована: Июнь 18, 2021
Язык: Английский
Процитировано
1130
Cell Biology International, Год журнала: 2021, Номер 45(10), С. 2045 - 2053
Опубликована: Июнь 28, 2021
Abstract Coronavirus disease 2019 (COVID‐19) is the seventh member of bat severe acute respiratory syndrome family. COVID‐19 can fuse their envelopes with host cell membranes and deliver genetic material. attacks system stimulates inflammatory responses, enhances recruitment immune cells, promotes angiotensin‐converting enzyme 2 activities. Patients confirmed may have experienced fever, dry cough, headache, dyspnea, kidney injury, distress syndrome, heart injury. Several strategies such as oxygen therapy, ventilation, antibiotic or antiviral renal replacement therapy are commonly used to decrease COVID‐19‐associated mortality. However, these approaches not be good treatment options. Therefore, search for an alternative‐novel urgently important prevent progression. Recently, microRNAs (miRNAs) emerged a promising strategy COVID‐19. The design oligonucleotide against material might suppress virus RNA translation. previous studies shown that miRNAs play role improve patients by binding 3′‐untranslated region (UTR) 5′‐UTR viral in COVID‐19‐host interplay replication. interact multiple pathways reduce biomarkers, thrombi formation, tissue damage accelerate patient outcome. information this review provides summary current clinical application treatments
Язык: Английский
Процитировано
78
Signal Transduction and Targeted Therapy, Год журнала: 2021, Номер 6(1)
Опубликована: Авг. 11, 2021
Elderly people and patients with comorbidities are at higher risk of COVID-19 infection, resulting in severe complications high mortality. However, the underlying mechanisms unclear. In this study, we investigate whether miRNAs serum exosomes can exert antiviral functions affect response to elderly diabetes. First, identified four (miR-7-5p, miR-24-3p, miR-145-5p miR-223-3p) through high-throughput sequencing quantitative real-time PCR analysis, that remarkably decreased diabetic groups. We further demonstrated these miRNAs, either exosome or free form, directly inhibit S protein expression SARS-CoV-2 replication. Serum from young replication expression, while inhibitory effect is markedly patients. Moreover, three out circulating significantly increased healthy volunteers after 8-weeks' continuous physical exercise. isolated also showed stronger effects on Our study demonstrates for first time exosomal may provide a possible explanation difference between comorbidities.
Язык: Английский
Процитировано
60
Cells, Год журнала: 2022, Номер 11(13), С. 1989 - 1989
Опубликована: Июнь 21, 2022
Extracellular vesicles (EVs) include a heterogeneous group of natural cell-derived nanostructures that are increasingly regarded as promising biotherapeutic agents and drug delivery vehicles in human medicine. Desirable intrinsic properties EVs including the ability to bypass membranous barriers deliver their unique biomolecular cargo specific cell populations position them fiercely competitive alternatives for currently available therapies artificial platforms. with distinct characteristics can be released from various types into extracellular environment means transmitting bioactive components altering status target cell. Despite existence large number preclinical studies confirming therapeutic efficacy different originated treating several pathological conditions, this review, we first provide brief overview EV biophysical an emphasis on benefits over cell-based synthetic systems. Next, describe detail derived sources, compare advantages disadvantages, recapitulate effects disorders highlight progress made harnessing clinical applications. Finally, knowledge gaps concrete hurdles hinder translation debated futuristic perspective.
Язык: Английский
Процитировано
45
International Journal of Nanomedicine, Год журнала: 2023, Номер Volume 18, С. 3177 - 3210
Опубликована: Июнь 1, 2023
Abstract: Exosomes are nanovesicles with a wide range of chemical compositions used in many different applications. Mesenchymal stem cell-derived exosomes (MSCs-EXOs) spherical vesicles that have been shown to mediate tissue regeneration variety diseases, including neurological, autoimmune and inflammatory, cancer, ischemic heart disease, lung injury, liver fibrosis. They can modulate the immune response by interacting effector cells due presence anti-inflammatory compounds involved intercellular communication through various types cargo. MSCs-EXOs exhibit cytokine storm-mitigating properties COVID-19. This review discussed potential function diseases notably epileptic encephalopathy Parkinson's angiogenesis, inflammatory diseases. We provided an overview exosome biogenesis factors regulate biogenesis. Additionally, we highlight functions use treatment disease Finally, covered strategies challenges MSCs-EXOs. discuss conclusion future perspectives Keywords: extracellular vesicle, biogenesis, mesenchymal cells, exosomes, angiogenesis
Язык: Английский
Процитировано
38
Journal of Extracellular Vesicles, Год журнала: 2024, Номер 13(12)
Опубликована: Дек. 1, 2024
Abstract Extracellular vesicles (EVs) are heterogeneous entities secreted by cells into their microenvironment and systemic circulation. Circulating EVs carry functional small RNAs other molecular footprints from cell of origin, thus have evident applications in liquid biopsy, therapeutics, intercellular communication. Yet, the complete transcriptomic landscape is poorly characterized due to critical limitations including variable protocols used for EV‐RNA extraction, quality control, cDNA library preparation, sequencing technologies, bioinformatic analyses. Consequently, there a gap knowledge need standardized approach delineating EV‐RNAs. Here, we address these gaps describing following points (1) focusing on large canopy particles (EVPs), which includes, but not limited – exosomes EVs, lipoproteins, exomeres/supermeres, mitochondrial‐derived vesicles, RNA binding proteins, cell‐free DNA/RNA/proteins; (2) examining potential roles biogenesis EVPs; (3) discussing various methods technologies uncovering cargoes (4) presenting comprehensive list subtypes reported (5) different databases resources specific species; (6) reviewing established bioinformatics pipelines novel strategies reproducible EV transcriptomics analyses; (7) emphasizing significant gold standard identifying EV‐RNAs across studies; (8) finally, highlight current challenges, discuss possible solutions, present recommendations robust analyses EVP‐associated RNAs. Overall, seek provide clarity landscape, EVP‐RNAs. Detailed portrayal state EVP will lead better understanding how cargo EVPs can be modern targeted diagnostics therapeutics. For inclusion discussed this article, use terms large/small non‐vesicular extracellular (NVEPs), EPs as defined MISEV guidelines International Society Vesicles (ISEV).
Язык: Английский
Процитировано
12
Inflammation and Regeneration, Год журнала: 2024, Номер 44(1)
Опубликована: Май 28, 2024
Abstract Background/aims Extracellular vesicles (EVs) derived from dental pulp mesenchymal stem cells (DP-MSCs) are a promising therapeutic option for the treatment of myocardial ischemia. The aim this study is to determine whether MSC-EVs could promote pro-resolving environment in heart by modulating macrophage populations. Methods EVs three independent biopsies DP-MSCs (MSC-EVs) were isolated tangential flow-filtration and size exclusion chromatography characterized omics analyses. Biological processes associated with these molecules analyzed using String GeneCodis platforms. immunomodulatory capacity polarize macrophages towards or M2-like phenotype was assessed evaluating surface markers, cytokine production, efferocytosis. potential evaluated an acute infarction (AMI) model nude rats. Infarct distribution populations infarct area 7 21 days after intramyocardial injection MSC-EVs. Results Lipidomic, proteomic, miRNA-seq analysis revealed their association biological involved tissue regeneration regulation immune system, among others. promoted differentiation pro-inflammatory phenotype, as evidenced increased expression M2 markers decreased secretion cytokines. Administration rats AMI limited extent infarcted at post-infarction. MSC-EV also reduced number within area, promoting resolution inflammation. Conclusion exhibited similar characteristics level irrespective biopsy which they derived. All exerted effective responses rat AMI, indicating agents inflammation AMI.
Язык: Английский
Процитировано
9
Biomedicines, Год журнала: 2021, Номер 9(6), С. 667 - 667
Опубликована: Июнь 10, 2021
Mesenchymal stem cells (MSCs), the distributed in stromas of body, are known for various properties including replication, potential differentiations, immune-related processes inflammation. About two decades ago, these were shown to play relevant roles therapy numerous diseases, dependent on their immune regulation and release cytokines growth factors, with ensuing activation favorable enzymes processes. Such discovery induced great increase investigation. Soon thereafter, however, it became clear that therapeutic actions MSCs risky, accompanied by serious drawbacks defects. MSC has been therefore reduced a few replaced others extracellular vesicles, MSC-EVs. The latter vesicles recapitulate most MSCs, equal or even better efficacies without parent cells. In addition, MSC-EVs characterized many advantages, among which heterogeneities origin, alleviation cell aging, responses Here we illustrate MSC-EV effects, largely mediated specific miRNAs, covering diseases pathological occurring bones, heart vessels, kidney, brain. operate also development cancers COVID-19, where they alleviate organ lesions virus. Therapy can be improved combination innate engineering inducing precise targeting transfer drugs. unique explain intense studies, carried out extraordinary success. Although not yet developed clinical practice, perspectives proximal future encouraging.
Язык: Английский
Процитировано
53
Reviews in Medical Virology, Год журнала: 2021, Номер 32(2)
Опубликована: Авг. 7, 2021
Summary Severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2) is causing an ongoing pandemic of disease 2019 (Covid‐19). Effective therapies are required for the treatment patients with severe stages disease. Mesenchymal stem cells (MSCs) have been evaluated in numerous clinical trials, but present challenges, such as carcinogenic risk and special storage conditions, coupled insufficient data about their mechanism action. The majority unique properties MSCs related to paracrine activity especially exosomes. impact MSCs‐derived exosomes (MSC‐Es) on complications Covid‐19 has investigated several studies. MSC‐Es may improve some cytokine storm, distress (ARDS) lung injury (ALI). Additionally, these can be applicable nano‐size carrier antiviral therapeutic agents. Herein, we consider potential applications derived Covid‐19.
Язык: Английский
Процитировано
48
Stem Cell Research & Therapy, Год журнала: 2021, Номер 12(1)
Опубликована: Авг. 21, 2021
Novel coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus-2. The virus causes an exaggerated immune response, resulting in a cytokine storm and distress syndrome, the leading cause of COVID-19-related mortality morbidity. So far, no therapies have succeeded circumventing exacerbated response or associated with COVID-19. Mesenchymal stem cells (MSCs), through their immunomodulatory regenerative activities, mostly mediated paracrine effect extracellular vesicle production, therapeutic potential many autoimmune, inflammatory, degenerative diseases. In this paper, we review clinical studies on use MSCs for COVID-19 treatment, including salutary effects pathophysiology immunomodulation storm. Ongoing trial designs, cell sources, dose administration, populations are summarized, mode benefit discussed. We also offer suggestions optimizing MSC-based therapies, genetic engineering, strategies surface modification, nanotechnology applications, combination therapies.
Язык: Английский
Процитировано
42