A Review on Mastitis in Dairy Cows Research: Current Status and Future Perspectives

Agriculture, Год журнала: 2024, Номер 14(8), С. 1292 - 1292

Опубликована: Авг. 5, 2024

One of the most serious diseases affecting dairy cattle, causing significant losses both in breeding and economy, is mastitis, an inflammation mammary gland. Due to economic importance this issue, many research teams are striving develop easy-to-apply and, importantly, effective method prevent mastitis. The use traditional methods for mastitis detecting treating, as well improvement hygienic conditions, have not yielded expected results combating disease combating. Currently, main task find tools that would allow rapid detection udder health cows while maintaining high milk production, which essential profitability cattle farming. Accurate diagnostic tools, with simultaneous capability identifying pathogens, may help reduce losses. Sufficient sensitivity specificity tests required minimize number false-positive false-negative cases. Efforts also being made determine optimal threshold value at its earliest possible stage. estimation somatic cell count (SCC) a phenotypic indicator widely used. A more precise parameter accurately describing differential (DSCC). well-known California Mastitis Test (CMT) inexpensive, easy, useful on farms. latest utilize based activity N-acetyl-β-d-glucosaminidase (NAGase) or lactate dehydrogenase (LDH) determination acute phase proteins (APPs) blood serum (such haptoglobin, amyloid A, fibrinogen, ceruloplasmin). Research focuses genomic resistance successive generations, purpose, quantitative trait loci (QTLs) single nucleotide polymorphisms (SNPs) been identified. In recent years, immunotherapy has become increasingly common area research, including vaccinations, T/B immunotherapy, RNA epigenetic stem therapy, native secretory factors. An important aspect control implementation strategies focus primarily preventing through appropriate farm management practices. forthcoming challenge will be development universal therapeutic can effectively implemented alternatives antibiotic therapy. Future should prioritize advancement preventive techniques, such immunotherapies, bacteriocins, herbal nanoparticle technology.

Язык: Английский

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Structurally and mechanically tuned macroporous hydrogels for scalable mesenchymal stem cell–extracellular matrix spheroid production

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(28)

Опубликована: Июль 2, 2024

Mesenchymal stem cells (MSCs) are essential in regenerative medicine. However, conventional expansion and harvesting methods often fail to maintain the extracellular matrix (ECM) components, which crucial for their functionality efficacy therapeutic applications. Here, we introduce a bone marrow-inspired macroporous hydrogel designed large-scale production of MSC–ECM spheroids. Through soft-templating approach leveraging liquid–liquid phase separation, engineer hydrogels with customizable features, including pore size, stiffness, bioactive ligand distribution, enzyme-responsive degradability. These tailored environments conducive optimal MSC proliferation ease harvesting. We find that soft enhance mechanotransduction MSCs, establishing standard hydrogel-based 3D cell culture. Within these hydrogels, MSCs exist as both cohesive spheroids, preserving innate vitality, migrating entities actively secrete functional ECM proteins. Additionally, also gentle, enzymatic method breaks down allowing secreted naturally form spheroids display heightened stemness differentiation capacity, mirroring benefits native milieu. Our research underscores significance sophisticated materials design nurturing distinct subpopulations, facilitating generation enhanced potential.

Язык: Английский

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Optimizing Stem Cell Expansion: The Role of Substrate Stiffness in Enhancing DPSC Quiescence and Regeneration

Journal of Endodontics, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Effect of in vivo culture conditions on the proliferation and differentiation of rat adipose-derived stromal cells

Mechanisms of Ageing and Development, Год журнала: 2024, Номер 219, С. 111935 - 111935

Опубликована: Апрель 12, 2024

Язык: Английский

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Secretome from iPSC-derived MSCs exerts proangiogenic and immunosuppressive effects to alleviate radiation-induced vascular endothelial cell damage

Stem Cell Research & Therapy, Год журнала: 2024, Номер 15(1)

Опубликована: Июль 29, 2024

Abstract Background Radiation therapy is the standard of care for central nervous system tumours. Despite success radiation in reducing tumour mass, irradiation (IR)-induced vasculopathies and neuroinflammation contribute to late-delayed complications, neurodegeneration, premature ageing long-term cancer survivors. Mesenchymal stromal cells (MSCs) are adult stem that facilitate tissue integrity, homeostasis, repair. Here, we investigated potential iPSC-derived MSC (iMSC) secretome immunomodulation vasculature repair response injury utilizing human cell lines. Methods We generated iMSC lines evaluated their conditioned media (iMSC CM) treat IR-induced injuries monocytes (THP1) brain vascular endothelial (hCMEC/D3). further assessed factors secretome, modulation, molecular pathways they elicit. Results Increasing doses IR disturbed tube spheroid formation hCMEC/D3. When IR-injured hCMEC/D3 (IR ≤ 5 Gy) were treated with CM, viability, adherence, compactness, proangiogenic sprout significantly ameliorated, ROS levels reduced. CM augmented cocultures iMSCs . Consistently, facilitated angiogenesis a zebrafish model vivo. Furthermore, suppressed NFκB activation, TNF-α release, production THP1 cells. Additionally, diminished NF-kB activation cocultured irradiated hCMEC/D3, iMSCs, or HMC3 microglial The cytokine array revealed contains immunosuppressive MCP1/CCL2, IL6, IL8/CXCL8, ANG (Angiogenin), GROα/CXCL1, RANTES/CCL5. Common promoter regulatory elements enriched TF-binding motifs such as androgen receptor (ANDR) GATA2. phosphokinome profiling increased expression pro-survival factors, PI3K/AKT/mTOR modulator PRAS40 β-catenin CM. transcriptome analysis GATA2 enrichment involved RNA metabolism, translation, mitochondrial respiration, DNA damage repair, neurodevelopment. Conclusions comodulated composite has alleviate radiation-induced immune activation.

Язык: Английский

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Early Stages of Ex Vivo Collagen Glycation Disrupt the Cellular Interaction and Its Remodelling by Mesenchymal Stem Cells—Morphological and Biochemical Evidence

Опубликована: Фев. 26, 2024

Mesenchymal stem cells (MSCs), whose main function is tissue repair, use collagen to restore the structural integrity of damaged tissue, maintaining its organization through concomitant remodelling. The non-enzymatic glycation likely compromise communication with MSC, which indeed underlies various pathological conditions such as late complications diabetes and aging. However, data on effect more early stages MSC interaction are lacking. This study focused fate in vitro glycated rat tail (RTC) contact MSCs after pre-exposure glucose for 1 or 5 days. Using human adipose tissue-derived (ADMSCs), we showed that their significantly altered, manifested morphologically by reduced cell spreading, less formation focal adhesions, weaker development actin cytoskeleton, further confirmed ImageJ morphometric analysis. suggests a poorer recognition integrin receptors, possibly due steric hindrance binding sites. These morphological events were also accompanied greatly fibril-like reorganization adsorbed FITC-collagen (a sign impaired remodeling), complemented sensitivity proteases. latter was two ways: measuring directly degradation attached quantifying proteolysis reduction upon exogenous addition collagenase cell-free system. mechanism observed effects unclear, although differential scanning calorimetry presence weak changes collagen. All this led us conclude reason ADMSCs early-glycated complementary sequences integrins, certainly affects mechanical remodeling collagenolytic activity, together small thermal transition profile, undoubtedly indicate some internal molecule occurring even at stage glycation, turn contributes activity.

Язык: Английский

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Engineering cell-derived extracellular matrix for peripheral nerve regeneration

Materials Today Bio, Год журнала: 2024, Номер 27, С. 101125 - 101125

Опубликована: Июнь 13, 2024

Extracellular matrices (ECMs) play a key role in nerve repair and are recognized as the natural source of biomaterials. In parallel to extensively studied tissue-derived ECMs (ts-ECMs), cell-derived (cd-ECMs) also have capability partially recapitulate complicated regenerative microenvironment native tissues. Notably, cd-ECMs can avoid shortcomings ts-ECMs. Cd-ECMs be prepared by culturing various cells or even autologous vitro under pathogen-free conditions. And mild decellularization achieve efficient removal immunogenic components cd-ECMs. Moreover, more readily customizable desired functional properties. These advantages garnered significant attention for potential neuroregenerative medicine. As promising biomaterials, bring new hope effective treatment peripheral injuries. Herein, this review comprehensively examines current knowledge about characteristics their mechanisms interaction with regeneration, particular focus on preparation, engineering optimization, scalability The applications from distinct cell sources reported tissue highlighted summarized. Furthermore, limitations that should addressed outlooks related clinical translation put forward well.

Язык: Английский

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3D modular bioceramic scaffolds for the investigation of the interaction between osteosarcoma cells and MSCs

Acta Biomaterialia, Год журнала: 2024, Номер 184, С. 431 - 443

Опубликована: Июнь 17, 2024

Язык: Английский

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Ovarian Stem Cells: Will the Dream of Neo-Folliculogenesis After Birth Become Real?

Obstetrical & Gynecological Survey, Год журнала: 2025, Номер 80(2), С. 112 - 120

Опубликована: Фев. 1, 2025

Ovarian stem cells (OSCs) represent a promising tool in reproductive medicine, particularly for the treatment of premature ovarian failure and fertility preservation. Herein, we summarize main characteristics adult cells, their status, needs, new challenges application medicine. Clinical studies have shown that OSCs transplantation can restore function stimulate neo-folliculogenesis patients with failure, enabling them to conceive naturally or through vitro fertilization techniques. Moreover, gained increasing interest as chance preserve cancer undergoing gonadotoxic treatments affecting fertility, chemotherapy radiotherapy. The recruitment from fresh thawed fragments coupled capability differentiate mature oocytes could provide novel opportunity verify suitability be expanded oocyte like cells. Research into applications medicine is still its infancy, but results so far are offer possibilities suffering cancer.

Язык: Английский

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Adhesion of Mesenchymal Stem Cells to Glycated Collagen—Comparative Analysis of Dynamic and Static Conditions

Polymers, Год журнала: 2025, Номер 17(6), С. 821 - 821

Опубликована: Март 20, 2025

Understanding mesenchymal stem cell (MSC) behavior on glycated collagen is crucial for advancing regenerative medicine and understanding pathological mechanisms in diseases such as diabetes, cancer, aging. While previous research has demonstrated reduced MSC interaction with under static conditions due to disrupted integrin signaling, these studies did not accurately replicate the dynamic mechanical environment that MSCs encounter vivo. Here we present a comprehensive investigation comparing adipose-derived (ADMSC) both flow adhesion, revealing unexpected temporal dynamics challenging existing paradigms of cell–matrix interactions. Using sophisticated microfluidic BioFlux system combined traditional adhesion assays, examined ADMSC interactions native 1-day (GL1), 5-day (GL5) samples. Under conditions, remarkably rapid attachment—within 3–5 min—contrasting sharply classical 2 h incubation protocol. This was particularly enhanced collagen, though subsequent testing revealed significantly weaker strength shear stress compared collagen. Static also showed distinct pattern: increased samples within first 30 min, followed by progressive decrease compromised spreading over longer periods. Atomic force microscopy (AFM) analysis significant changes surface properties upon glycation. These included substantial reduction negative charge (from ~800 600 mV), altered roughness patterns (Rrms varying from 3.0 ± 0.4 nm 7.70 0.6 GL5), decreased elasticity (Young’s modulus dropping 34.8 5.4 MPa 2.07 0.3 GL5). physical alterations appear facilitate initial attachment while potentially compromising long-term stable through integrin-mediated mechanisms. study provides novel insights into complex previously unknown models The findings suggest need revised approaches tissue engineering medicine, where prevalent.

Язык: Английский

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