Structurally and mechanically tuned macroporous hydrogels for scalable mesenchymal stem cell–extracellular matrix spheroid production

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(28)

Published: July 2, 2024

Mesenchymal stem cells (MSCs) are essential in regenerative medicine. However, conventional expansion and harvesting methods often fail to maintain the extracellular matrix (ECM) components, which crucial for their functionality efficacy therapeutic applications. Here, we introduce a bone marrow-inspired macroporous hydrogel designed large-scale production of MSC–ECM spheroids. Through soft-templating approach leveraging liquid–liquid phase separation, engineer hydrogels with customizable features, including pore size, stiffness, bioactive ligand distribution, enzyme-responsive degradability. These tailored environments conducive optimal MSC proliferation ease harvesting. We find that soft enhance mechanotransduction MSCs, establishing standard hydrogel-based 3D cell culture. Within these hydrogels, MSCs exist as both cohesive spheroids, preserving innate vitality, migrating entities actively secrete functional ECM proteins. Additionally, also gentle, enzymatic method breaks down allowing secreted naturally form spheroids display heightened stemness differentiation capacity, mirroring benefits native milieu. Our research underscores significance sophisticated materials design nurturing distinct subpopulations, facilitating generation enhanced potential.

Language: Английский

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A Review on Mastitis in Dairy Cows Research: Current Status and Future Perspectives

Agriculture, Journal Year: 2024, Volume and Issue: 14(8), P. 1292 - 1292

Published: Aug. 5, 2024

One of the most serious diseases affecting dairy cattle, causing significant losses both in breeding and economy, is mastitis, an inflammation mammary gland. Due to economic importance this issue, many research teams are striving develop easy-to-apply and, importantly, effective method prevent mastitis. The use traditional methods for mastitis detecting treating, as well improvement hygienic conditions, have not yielded expected results combating disease combating. Currently, main task find tools that would allow rapid detection udder health cows while maintaining high milk production, which essential profitability cattle farming. Accurate diagnostic tools, with simultaneous capability identifying pathogens, may help reduce losses. Sufficient sensitivity specificity tests required minimize number false-positive false-negative cases. Efforts also being made determine optimal threshold value at its earliest possible stage. estimation somatic cell count (SCC) a phenotypic indicator widely used. A more precise parameter accurately describing differential (DSCC). well-known California Mastitis Test (CMT) inexpensive, easy, useful on farms. latest utilize based activity N-acetyl-β-d-glucosaminidase (NAGase) or lactate dehydrogenase (LDH) determination acute phase proteins (APPs) blood serum (such haptoglobin, amyloid A, fibrinogen, ceruloplasmin). Research focuses genomic resistance successive generations, purpose, quantitative trait loci (QTLs) single nucleotide polymorphisms (SNPs) been identified. In recent years, immunotherapy has become increasingly common area research, including vaccinations, T/B immunotherapy, RNA epigenetic stem therapy, native secretory factors. An important aspect control implementation strategies focus primarily preventing through appropriate farm management practices. forthcoming challenge will be development universal therapeutic can effectively implemented alternatives antibiotic therapy. Future should prioritize advancement preventive techniques, such immunotherapies, bacteriocins, herbal nanoparticle technology.

Language: Английский

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Optimizing Stem Cell Expansion: The Role of Substrate Stiffness in Enhancing DPSC Quiescence and Regeneration

Journal of Endodontics, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Effect of in vivo culture conditions on the proliferation and differentiation of rat adipose-derived stromal cells

Mechanisms of Ageing and Development, Journal Year: 2024, Volume and Issue: 219, P. 111935 - 111935

Published: April 12, 2024

Language: Английский

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Ovarian Stem Cells: Will the Dream of Neo-Folliculogenesis After Birth Become Real?

Obstetrical & Gynecological Survey, Journal Year: 2025, Volume and Issue: 80(2), P. 112 - 120

Published: Feb. 1, 2025

Ovarian stem cells (OSCs) represent a promising tool in reproductive medicine, particularly for the treatment of premature ovarian failure and fertility preservation. Herein, we summarize main characteristics adult cells, their status, needs, new challenges application medicine. Clinical studies have shown that OSCs transplantation can restore function stimulate neo-folliculogenesis patients with failure, enabling them to conceive naturally or through vitro fertilization techniques. Moreover, gained increasing interest as chance preserve cancer undergoing gonadotoxic treatments affecting fertility, chemotherapy radiotherapy. The recruitment from fresh thawed fragments coupled capability differentiate mature oocytes could provide novel opportunity verify suitability be expanded oocyte like cells. Research into applications medicine is still its infancy, but results so far are offer possibilities suffering cancer.

Language: Английский

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Adhesion of Mesenchymal Stem Cells to Glycated Collagen—Comparative Analysis of Dynamic and Static Conditions

Polymers, Journal Year: 2025, Volume and Issue: 17(6), P. 821 - 821

Published: March 20, 2025

Understanding mesenchymal stem cell (MSC) behavior on glycated collagen is crucial for advancing regenerative medicine and understanding pathological mechanisms in diseases such as diabetes, cancer, aging. While previous research has demonstrated reduced MSC interaction with under static conditions due to disrupted integrin signaling, these studies did not accurately replicate the dynamic mechanical environment that MSCs encounter vivo. Here we present a comprehensive investigation comparing adipose-derived (ADMSC) both flow adhesion, revealing unexpected temporal dynamics challenging existing paradigms of cell–matrix interactions. Using sophisticated microfluidic BioFlux system combined traditional adhesion assays, examined ADMSC interactions native 1-day (GL1), 5-day (GL5) samples. Under conditions, remarkably rapid attachment—within 3–5 min—contrasting sharply classical 2 h incubation protocol. This was particularly enhanced collagen, though subsequent testing revealed significantly weaker strength shear stress compared collagen. Static also showed distinct pattern: increased samples within first 30 min, followed by progressive decrease compromised spreading over longer periods. Atomic force microscopy (AFM) analysis significant changes surface properties upon glycation. These included substantial reduction negative charge (from ~800 600 mV), altered roughness patterns (Rrms varying from 3.0 ± 0.4 nm 7.70 0.6 GL5), decreased elasticity (Young’s modulus dropping 34.8 5.4 MPa 2.07 0.3 GL5). physical alterations appear facilitate initial attachment while potentially compromising long-term stable through integrin-mediated mechanisms. study provides novel insights into complex previously unknown models The findings suggest need revised approaches tissue engineering medicine, where prevalent.

Language: Английский

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Topological defects induced intra-tissue heterogeneity of mesenchymal stem cell via regulatory self-organization and differentiation

Biomaterials Advances, Journal Year: 2025, Volume and Issue: unknown, P. 214297 - 214297

Published: April 1, 2025

Language: Английский

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The bone microenvironment: new insights into the role of stem cells and cell communication in bone regeneration

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 12, 2025

Mesenchymal stem cells (MSCs) play a crucial role in bone formation and remodeling. Intrinsic genetic factors extrinsic environmental cues regulate their differentiation into osteoblasts. Within the microenvironment, complex network of biochemical biomechanical signals orchestrates homeostasis regeneration. In addition, crosstalk among MSCs, immune cells, neighboring cells-mediated by extracellular vesicles non-coding RNAs (such as circular micro RNAs) -profoundly influences osteogenic Recent studies have explored specific signaling pathways that contribute to effective regeneration, highlighting potential manipulating microenvironment enhance MSC functionality. The integration advanced biomaterials, gene editing techniques, controlled delivery systems is paving way for more targeted efficient regenerative therapies. Furthermore, artificial intelligence could improve tissue engineering, optimize biomaterial design, enable personalized treatment strategies. This review explores latest advancements emphasizing intricate interplay molecules. By providing comprehensive overview these mechanisms clinical implications, we aim shed light on future research directions this rapidly evolving field.

Language: Английский

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Early Stages of Ex Vivo Collagen Glycation Disrupt the Cellular Interaction and Its Remodelling by Mesenchymal Stem Cells—Morphological and Biochemical Evidence

Published: Feb. 26, 2024

Mesenchymal stem cells (MSCs), whose main function is tissue repair, use collagen to restore the structural integrity of damaged tissue, maintaining its organization through concomitant remodelling. The non-enzymatic glycation likely compromise communication with MSC, which indeed underlies various pathological conditions such as late complications diabetes and aging. However, data on effect more early stages MSC interaction are lacking. This study focused fate in vitro glycated rat tail (RTC) contact MSCs after pre-exposure glucose for 1 or 5 days. Using human adipose tissue-derived (ADMSCs), we showed that their significantly altered, manifested morphologically by reduced cell spreading, less formation focal adhesions, weaker development actin cytoskeleton, further confirmed ImageJ morphometric analysis. suggests a poorer recognition integrin receptors, possibly due steric hindrance binding sites. These morphological events were also accompanied greatly fibril-like reorganization adsorbed FITC-collagen (a sign impaired remodeling), complemented sensitivity proteases. latter was two ways: measuring directly degradation attached quantifying proteolysis reduction upon exogenous addition collagenase cell-free system. mechanism observed effects unclear, although differential scanning calorimetry presence weak changes collagen. All this led us conclude reason ADMSCs early-glycated complementary sequences integrins, certainly affects mechanical remodeling collagenolytic activity, together small thermal transition profile, undoubtedly indicate some internal molecule occurring even at stage glycation, turn contributes activity.

Language: Английский

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Engineering cell-derived extracellular matrix for peripheral nerve regeneration

Materials Today Bio, Journal Year: 2024, Volume and Issue: 27, P. 101125 - 101125

Published: June 13, 2024

Extracellular matrices (ECMs) play a key role in nerve repair and are recognized as the natural source of biomaterials. In parallel to extensively studied tissue-derived ECMs (ts-ECMs), cell-derived (cd-ECMs) also have capability partially recapitulate complicated regenerative microenvironment native tissues. Notably, cd-ECMs can avoid shortcomings ts-ECMs. Cd-ECMs be prepared by culturing various cells or even autologous vitro under pathogen-free conditions. And mild decellularization achieve efficient removal immunogenic components cd-ECMs. Moreover, more readily customizable desired functional properties. These advantages garnered significant attention for potential neuroregenerative medicine. As promising biomaterials, bring new hope effective treatment peripheral injuries. Herein, this review comprehensively examines current knowledge about characteristics their mechanisms interaction with regeneration, particular focus on preparation, engineering optimization, scalability The applications from distinct cell sources reported tissue highlighted summarized. Furthermore, limitations that should addressed outlooks related clinical translation put forward well.

Language: Английский

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