AJP Cell Physiology,
Год журнала:
2018,
Номер
314(6), С. C690 - C701
Опубликована: Фев. 21, 2018
LncRNA
homeobox
(HOX)
transcript
antisense
intergenic
RNA
(HOTAIR)
has
been
confirmed
to
be
involved
in
the
tumorigenic
progression
of
endometrial
carcinoma
(EC).
However,
molecular
mechanisms
HOTAIR
EC
are
not
fully
elucidated.
The
expression
and
miR-646
human
tissues
was
determined
by
qRT-PCR.
effect
on
cells
assessed
cell
viability,
migration,
invasion
using
CCK-8
assays
transwell
assays.
RNA-binding
protein
immunoprecipitation
pull-down
were
performed
explore
interaction
between
miR-646.
regulation
nucleophosmin
1
(NPM1)
tested
luciferase
reporter
MiR-646
significantly
decreased
both
(
n
=
23)
lines
(Ishikawa
HEC-1-A)
compared
with
control.
Moreover,
negatively
related
23).
Our
results
also
showed
that
overexpression
considerably
attenuated
E2-promoted
Ishikawa
HEC-1-A
vitro.
In
addition,
regulate
through
negative
regulating
More
importantly,
we
demonstrated
NPM1
target
miR-646,
promoted
interacting
cells.
Taken
together,
our
findings
presented
could
via
thereby
governing
Gastric
cancer
is
the
fourth
most
common
malignancy
and
third
leading
cause
of
cancer-related
deaths
worldwide.
Advanced
gastric
patients
can
notably
benefit
from
chemotherapy
including
adriamycin,
platinum
drugs,
5-fluorouracil,
vincristine,
paclitaxel
as
well
targeted
therapy
drugs.
Nevertheless,
primary
drug
resistance
or
acquisition
eventually
lead
to
treatment
failure
poor
outcomes
patients.
The
detailed
mechanisms
involved
in
have
been
revealed.
Interestingly,
different
noncoding
RNAs
(ncRNAs),
such
microRNAs
(miRNAs),
long
(lncRNAs)
circular
(circRNAs),
are
critically
development.
Multiple
lines
evidences
demonstrated
that
ncRNAs
play
a
vital
role
reagents
In
this
review,
we
systematically
summarized
emerging
molecular
impact
cancer.
Additionally,
propose
potential
clinical
implications
novel
therapeutic
targets
prognostic
biomarkers
for
International Journal of Biological Sciences,
Год журнала:
2018,
Номер
14(14), С. 2083 - 2093
Опубликована: Янв. 1, 2018
The
cellular
environment
where
tumor
cells
reside
is
called
the
microenvironment
(TME),
which
consists
of
borders,
blood
vessels,
lymph
extracellular
matrix
(ECM),
stromal
cells,
immune/inflammatory
secreted
proteins,
RNAs
and
small
organelles.By
dynamically
interacting
with
participate
in
all
stages
initiation,
progression,
metastasis,
recurrence
drug
response,
consequently,
affect
fate
patients.During
processes
evolution
metastasis
TME
also
experience
some
changes
play
roles
both
suppression
promotion
while
overall
function
beneficial
for
cancer
cell
survival
movement.In
this
review,
we
examine
effects
on
including
angiogenesis,
epithelial-mesenchymal
transition
(EMT)
invasion.We
highlight
functions
organelles
by
their
influences
multiple
metastasis.
Archives of Toxicology,
Год журнала:
2021,
Номер
95(7), С. 2279 - 2297
Опубликована: Май 18, 2021
Over
the
last
decade,
important
clinical
benefits
have
been
achieved
in
cancer
patients
by
using
drug-targeting
strategies.
Nevertheless,
drug
resistance
is
still
a
major
problem
most
therapies.
Epithelial-mesenchymal
plasticity
(EMP)
and
tumour
microenvironment
described
as
limiting
factors
for
effective
treatment
many
types.
Moreover,
epithelial-to-mesenchymal
transition
(EMT)
has
also
associated
with
therapy
different
preclinical
models,
although
limited
evidence
obtained
from
studies
samples.
In
this
review,
we
particularly
deepen
into
mechanisms
of
which
intermediate
epithelial/mesenchymal
(E/M)
states
its
interconnection
to
influence
resistance.
We
describe
how
use
bioinformatics
pharmacogenomics
will
help
figure
out
biological
impact
EMT
on
develop
novel
pharmacological
approaches
future.
Molecular Therapy — Nucleic Acids,
Год журнала:
2022,
Номер
28, С. 758 - 791
Опубликована: Апрель 27, 2022
Exosomes
are
small
extracellular
vesicles
with
sizes
ranging
from
30–150
nanometers
that
contain
proteins,
lipids,
mRNAs,
microRNAs,
and
double-stranded
DNA
derived
the
cells
of
origin.
can
be
taken
up
by
target
cells,
acting
as
a
means
cell-to-cell
communication.
The
discovery
these
in
body
fluids
their
participation
cell
communication
has
led
to
major
breakthroughs
diagnosis,
prognosis,
treatment
several
conditions
(e.g.,
cancer).
However,
conventional
isolation
evaluation
exosomes
microRNA
content
suffers
high
cost,
lengthy
processes,
difficult
standardization,
low
purity,
poor
yield.
emergence
microfluidics
devices
increased
efficiency
sieving,
trapping,
immunological
separation
volumes
could
provide
improved
detection
monitoring
involved
cancer.
Microfluidics
techniques
hold
promise
for
advances
development
diagnostic
prognostic
devices.
This
review
covers
ongoing
research
on
microRNAs
biomarkers
translation
point-of-care
clinical
applications.
Journal of Cellular Biochemistry,
Год журнала:
2017,
Номер
119(6), С. 4711 - 4716
Опубликована: Дек. 26, 2017
Abstract
Currently
available
studies
have
implicated
that
exosome‐delivered
microRNAs
(miRNAs)
play
crucial
roles
in
human
cancer.
However,
the
association
of
serum
exosomal
miR‐638
and
hepatocellular
carcinoma
(HCC)
remains
largely
unknown.
We
aim
to
investigate
expression
HCC
patients
its
prognostic
role
this
deadly
disease.
Kaplan‐Meier
Cox
regression
analyses
were
used
determine
survival
histologically
diagnosed
with
HCC.
Reduced
levels
samples
from
identified
by
real‐time
PCR.
Negative
tumor
size,
vascular
infiltration,
TNM
stage
was
observed
patients.
Besides,
proliferation
Huh7
SMCC7721
cells
significantly
inhibited
when
over‐expressed
these
cells.
In
addition,
lower
had
poor
overall
than
those
higher
serum.
Our
study
strongly
suggests
may
serve
as
a
novel
circulating
biomarker
for
Downregulation
predicts
prognosis
A
growing
body
of
evidence
indicates
that
exosomes
play
a
critical
role
in
the
cell–cell
communication
process.
Exosomes
are
biological
nanoparticles
with
an
average
diameter
30–100
nm
size
and
produced
by
almost
all
cell
types
human
body;
however,
cancer
cells
contain
higher
concentrations
than
healthy
cells.
They
released
into
fluids
double-stranded
DNA
(originated
from
nucleus
mitochondria),
variety
RNA
species,
specific
protein
biomarkers
can
be
utilized
as
therapeutic
targets,
lipids.
Therefore,
secreted
tumor
could
used
to
predict
existence
presence
patients.
This
review
summarizes
development
their
potential
utility
clinic.
