Bioactive peptides and proteins for tissue repair: microenvironment modulation, rational delivery, and clinical potential

Military Medical Research, Год журнала: 2024, Номер 11(1)

Опубликована: Дек. 5, 2024

Abstract Bioactive peptides and proteins (BAPPs) are promising therapeutic agents for tissue repair with considerable advantages, including multifunctionality, specificity, biocompatibility, biodegradability. However, the high complexity of microenvironments their inherent deficiencies such as short half-live susceptibility to enzymatic degradation, adversely affect efficacy clinical applications. Investigating fundamental mechanisms by which BAPPs modulate microenvironment developing rational delivery strategies essential optimizing administration in distinct repairs facilitating translation. This review initially focuses on through influence via reactive oxygen species, blood lymphatic vessels, immune cells, cells. Then, a variety platforms, scaffolds hydrogels, electrospun fibers, surface coatings, assisted particles, nanotubes, two-dimensional nanomaterials, nanoparticles engineered summarized incorporate effective repair, modification aimed at enhancing loading efficiencies release kinetics also reviewed. Additionally, can be precisely regulated endogenous stimuli (glucose, enzymes, pH) or exogenous (ultrasound, heat, light, magnetic field, electric field) achieve on-demand tailored specific needs. Furthermore, this potential across various types, bone, cartilage, intervertebral discs, muscle, tendons, periodontal tissues, skin, myocardium, nervous system (encompassing brain, spinal cord, peripheral nerve), endometrium, well ear ocular tissue. Finally, current challenges prospects discussed.

Язык: Английский

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Methacrylated Carboxymethyl Chitosan Scaffold Containing Icariin-Loaded Short Fibers for Antibacterial, Hemostasis, and Bone Regeneration

ACS Biomaterials Science & Engineering, Год журнала: 2024, Номер 10(8), С. 5181 - 5193

Опубликована: Июнь 27, 2024

Bone defects typically result in bone nonunion, delayed or nonhealing, and localized dysfunction, commonly used clinical treatments (i.e., autologous allogeneic grafts) have limited results. The multifunctional tissue engineering scaffold provides a new treatment for the repair of defects. Herein, three-dimensional porous composite with stable mechanical support, effective antibacterial hemostasis properties, ability to promote rapid was synthesized using methacrylated carboxymethyl chitosan icariin-loaded poly-l-lactide/gelatin short fibers (M-CMCS-SFs). Icariin-loaded SFs M-CMCS resulted sustained release osteogenic agents, which beneficial reinforcement. Both structure use facilitate absorption blood fluid exudates. Moreover, its superior properties could prevent occurrence inflammation infection. When cultured mesenchymal stem cells, showed promotion differentiation. Taken together, such comprehensive performance antibacterial, hemostasis, regeneration, thus holding promising potential related medical treatments.

Язык: Английский

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Advances in Nanofiber Cathodes for Aluminum-Ion Batteries

Advanced Fiber Materials, Год журнала: 2025, Номер unknown

Опубликована: Янв. 15, 2025

Язык: Английский

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Biomimetic Nanomaterials Based on Peptide In Situ Self‐Assembly for Immunotherapy Applications

Wiley Interdisciplinary Reviews Nanomedicine and Nanobiotechnology, Год журнала: 2025, Номер 17(1)

Опубликована: Янв. 1, 2025

ABSTRACT Cancer remains the leading cause of patient death worldwide and its incidence continues to rise. Immunotherapy is rapidly developing due significant differences in mechanism action from conventional radiotherapy targeted antitumor drugs. In past decades, many biomaterials have been designed prepared construct therapeutic platforms that modulate immune system against cancer. Immunotherapeutic utilizing can markedly enhance efficacy by optimizing delivery agents, minimizing drug loss during circulation, amplifying immunomodulatory effects. The intricate physiological barriers tumors, coupled with adverse environments such as inadequate infiltration, off‐target effects, immunosuppression, emerged obstacles impeding effectiveness oncology therapy. However, most current studies are devoted development complex immunomodulators exert functions loading drugs or adjuvants, ignoring tumors. Compared biomaterials, biomimetic nanomaterials based on peptide situ self‐assembly excellent functional characteristics biocompatibility, biodegradability, bioactivity a novel effective tool for cancer immunotherapy. This article presents comprehensive review latest research findings tumor Initially, we categorize structural types elucidate their intrinsic driving forces. Subsequently, delve into strategies these nanomaterials, highlighting advantages Furthermore, detail applications antigen presentation modulation microenvironment. conclusion, encapsulate challenges prospective developments clinical translation

Язык: Английский

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Electrospun Fiber Membranes Modified with Heparin and Parathyroid Hormone Derivative To Promote Diabetic Wound Healing

Macromolecular Bioscience, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

The repair of diabetic wounds is a global challenge due to elevated levels blood sugar and microvascular disorders. Parathyroid hormone (PTH) its derivatives show great potential for wound healing, but corresponding delivery strategies are still limited. Herein, novel electrospun fiber membrane whose surface modified with heparin fabricated deliver PTH derivative named parathyroid related peptide-1 (PTHrP-1) the healing wounds. Cell viability, proliferation, migration, proangiogenic activity first evaluated in vitro, then rat skin defect model used observe vivo effects on healing. Results vitro studies confirm that PTHrP-1 promotes proliferation migration endothelial cells fibroblasts, facilitates through angiogenesis, collagen deposition, re-epithelialization. In conclusion, shows promise delivering promoting

Язык: Английский

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Dynamic adaptive hydrogel facilitates neuroregeneration in segmental nerve deficits via immunomodulation and mitochondrial homeostasis

Chemical Engineering Journal, Год журнала: 2024, Номер 494, С. 152890 - 152890

Опубликована: Июнь 5, 2024

Язык: Английский

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GelMA hydrogels reinforced by PCL@GelMA nanofibers and bioactive glass induce bone regeneration in critical size cranial defects

Journal of Nanobiotechnology, Год журнала: 2024, Номер 22(1)

Опубликована: Ноя. 11, 2024

The process of bone healing is complex and involves the participation osteogenic stem cells, extracellular matrix, angiogenesis. advancement regeneration materials provides a promising opportunity to tackle defects. This study introduces composite hydrogel that can be injected cured using UV light. Hydrogels comprise bioactive glass (BG) PCL@GelMA coaxial nanofibers. addition BG nanofibers improves hydrogel's mechanical capabilities (353.22 ± 36.13 kPa) stability while decreasing its swelling (258.78 17.56%) hydration (72.07 1.44%) characteristics. demonstrates exceptional biocompatibility angiogenesis, enhances development in marrow mesenchymal cells (BMSCs), dramatically increases expression critical markers such as ALP, RUNX2, OPN. significantly (25.08 1.08%) non-healing calvaria defects promotes increased both marker (OPN) angiogenic (CD31) vivo. OPN CD31 was up 5 1.87 times higher than control group at 12 weeks. We successfully prepared novel injectable hydrogel, design hydrogels shows significant potential for enhancing biocompatibility, improving expression, has beneficial effect on producing an optimal microenvironment repair.

Язык: Английский

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Enhancing long-segmental tracheal restoration: A self-repairing hydrogel loaded with chondrocytokines for sutureless anastomosis and cartilage regeneration

Materials Today Bio, Год журнала: 2024, Номер 28, С. 101208 - 101208

Опубликована: Авг. 30, 2024

Artificial tracheal substitutes encounter significant challenges during long-segmental defects (LSTD) reconstruction, notably early postoperative anastomotic stenosis and chondromalacia. Mitigating by creating a compliant sutureless substitute is pivotal. Enhancing its chondrogenic capacity equally critical for sustained healthy cartilage regeneration. This study proposes self-healing hydrogel anastomosis to mitigate stenosis, enriched with kartogenin (KGN) transforming growth factor-β1 (TGFβ1) bolster properties. Initially, two precursor solutions were prepared: 1) aldehyde-modified hyaluronic acid sulfonation β-cyclodextrin-CHO loaded KGN; 2) hydrazide-grafted gelatin TGFβ1. Coextrusion of these resulted in gelated G + TGFβ1/sH-CD KGN hydrogel, characterized robust covalent bonding network acylhydrazones between hydrazide aldehyde groups, imparting excellent The hydrogels, showcasing favorable cytocompatibility, injectability, rapid gelation, bone marrow stem cells. These customized into O-shaped rings assembled malleable using our established ring-to-tube method. resultant facilitated LSTD rabbit model, attributed the Schiff base reaction hydrogel's carbonyl group tissue's amino group. Notably, reduced maintained patency, alleviated sputum blockage, promoted reepithelization, increased survival rate experimental rabbits. release chondrocytokines Employing chondrocytokines-loaded hydrogels properties represents advancement regeneration, holding promising potential inhibiting chondromalacia when treating LSTD.

Язык: Английский

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Temporal modulation of inflammation and chondrogenesis through dendritic nanoparticle-mediated therapy with diclofenac surface modification and strontium ion encapsulation

Journal of Biomaterials Science Polymer Edition, Год журнала: 2024, Номер 35(13), С. 2049 - 2067

Опубликована: Июль 12, 2024

Cartilage tissue engineering holds great promise for efficient cartilage regeneration. However, early inflammatory reactions to seed cells and/or scaffolds impede this process. Consequently, managing inflammation is of paramount importance. Moreover, due the body's restricted chondrogenic capacity, inducing regeneration becomes imperative. Thus, a controlled platform essential establish an anti-inflammatory microenvironment before initiating In study, we utilized fifth-generation polyamidoamine dendrimers (G5) as vehicle drugs create composite nanoparticles known G5-Dic/Sr. These were generated by surface modification with diclofenac (Dic), its potent effects, and encapsulating strontium (Sr), which effectively induces chondrogenesis, within core. Our findings indicated that G5-Dic/Sr nanoparticle exhibited selective Dic release during initial 9 days gradual Sr from 3 15. Subsequently, these incorporated into gelatin methacryloyl (GelMA) hydrogel, resulting in GelMA@G5-Dic/Sr.

Язык: Английский

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Shell-core structured nanofibers mediate staged anti-inflammatory and pro-neurogenic activities to repair peripheral nerve

Materials Research Express, Год журнала: 2024, Номер 11(8), С. 085006 - 085006

Опубликована: Авг. 1, 2024

Abstract The inflammatory reaction significantly impedes the neurogenic process during restoration of peripheral nerve injury (PNI). Therefore, establishing a non-inflammatory environment is crucial for effective regeneration. This study proposes use shell-core structured nanofibers with sequential anti-inflammatory and pro-neurogenic activities to repair PNI. Icariin (ICA), known its effects, was blended poly(lactic-co-glycolic acid) (PLGA) form shell layer’s spinning solution. Concurrently, glial cell-derived neurotrophic factor (GDNF) combined graphene oxide (GO) create core These solutions were then subjected co-axial electrospinning, resulting in GDNF@GO-ICA@PLGA nanofibers. Additionally, control group unordered GDNF/GO/ICA/PLGA prepared using conventional electrospinning. exhibited distinct fibrous structures clear architecture demonstrated mechanical properties similar group. Notably, displayed unique staged release kinetics: over 90% ICA released priorly within first 0 13 days, followed by GDNF from days 9 31. Furthermore, showed excellent biocompatibility Schwann cells. In vitro results highlighted potent capabilities layer, while layer effectively induced differentiation processed into conduit applied 10 mm rat sciatic PNI model. facilitated created before initiating regeneration, leading improved restoration. underscores importance sequentially mediating anti-inflammation neurogenesis, offering novel approach addressing

Язык: Английский

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