Vascularized tumor models for the evaluation of drug delivery systems: a paradigm shift

Drug Delivery and Translational Research, Год журнала: 2024, Номер 14(8), С. 2216 - 2241

Опубликована: Апрель 15, 2024

Abstract As the conversion rate of preclinical studies for cancer treatment is low, user-friendly models that mimic pathological microenvironment and drug intake with high throughput are scarce. Animal key, but an alternative to reduce their use would be valuable. Vascularized tumor-on-chip combine great versatility scalable easy use. Several strategies integrate both tumor vascular compartments have been developed, few used assess delivery. Permeability, intra/extravasation, free circulation often evaluated, imperfectly recapitulate processes at stake. Indeed, targeting chemoresistance bypass must investigated design promising therapeutics. In vitro help development delivery systems (DDS) thus needed. They allow selecting good candidates before animal based on rational criteria such as accumulation, diffusion in tumor, potency, well absence side damage. this review, we focus vascularized models. First, detail fabrication, especially materials, cell types, coculture used. Then, different vascularization described along classical applications intra/extravasation or assessment. Finally, current trends DDS discussed overview efforts domain. Graphical

Язык: Английский

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Recent Advancements in Deep Learning Using Whole Slide Imaging for Cancer Prognosis

Bioengineering, Год журнала: 2023, Номер 10(8), С. 897 - 897

Опубликована: Июль 28, 2023

This review furnishes an exhaustive analysis of the latest advancements in deep learning techniques applied to whole slide images (WSIs) context cancer prognosis, focusing specifically on publications from 2019 through 2023. The swiftly maturing field learning, combination with burgeoning availability WSIs, manifests significant potential revolutionizing predictive modeling prognosis. In light swift evolution and profound complexity field, it is essential systematically contemporary methodologies critically appraise their ramifications. elucidates prevailing landscape this intersection, cataloging major developments, evaluating strengths weaknesses, providing discerning insights into prospective directions. paper, a comprehensive overview aims be presented, which can serve as critical resource for researchers clinicians, ultimately enhancing quality care outcomes. review’s findings accentuate need ongoing scrutiny recent studies rapidly progressing discern patterns, understand breakthroughs, navigate future research trajectories.

Язык: Английский

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Printing of 3D biomimetic structures for the study of bone metastasis: A review

Acta Biomaterialia, Год журнала: 2024, Номер 178, С. 24 - 40

Опубликована: Март 7, 2024

Bone metastasis primarily occurs when breast, prostate, or lung cancers disseminate tumoral cells into bone tissue, leading to a range of complications in skeletal tissues and, severe cases, paralysis resulting from spinal cord compression. Unfortunately, our understanding pathophysiological mechanisms is incomplete and the translation research clinic has been slow, mainly due lack credible ex vivo models study disease progression. Development reliable rational how tumor become circulating then invade sequentially colonize are great need. Advances tissue engineering technologies offers 3D alternatives which answer relevant questions towards cancer evolution key functional properties progression as well prognosis therapeutic approach. Here we performed an overview cellular involved including short summary normal physiology initiation Also, comprehensively summarized current advances methodologies fabrication based on state-of-the-art printing recapitulate structural biological features native tissue. STATEMENT OF SIGNIFICANCE: This review provides comprehensive collective findings relation various printed utilized for investigating specific diseases, related characteristic functions chemotherapeutic drug screening. These evaluated compared, terms their ability physiological microenvironment. Various biomaterials (natural synthetic polymers ceramic substrates) strategies design architecture used discussed here. clearly out-lines challenges prospects by focusing required perspective clinical application these

Язык: Английский

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A systematic review on the culture methods and applications of 3D tumoroids for cancer research and personalized medicine

Cellular Oncology, Год журнала: 2024, Номер unknown

Опубликована: Май 28, 2024

Cancer is a highly heterogeneous disease, and thus treatment responses vary greatly between patients. To improve therapy efficacy outcome for cancer patients, more representative patient-specific preclinical models are needed. Organoids tumoroids 3D cell culture that typically retain the genetic epigenetic characteristics, as well morphology, of their tissue origin. Thus, they can be used to understand underlying mechanisms initiation, progression, metastasis in physiological setting. Additionally, co-culture methods cancer-associated cells help interplay tumor its microenvironment. In recent years, have already helped refine treatments identify new targets therapy. Advanced culturing systems such chip-based fluidic devices bioprinting combination with been high-throughput applications personalized medicine. Even though organoid tumoroid complex vitro systems, validation results vivo still common practice. Here, we describe how both animal- human-derived novel vulnerabilities currently precision

Язык: Английский

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An engineered model of metastatic colonization of human bone marrow reveals breast cancer cell remodeling of the hematopoietic niche

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(42)

Опубликована: Окт. 7, 2024

Incomplete understanding of metastatic disease mechanisms continues to hinder effective treatment cancer. Despite remarkable advancements toward the identification druggable targets, options for patients in remission following primary tumor resection remain limited. Bioengineered human tissue models sites capable recreating physiologically relevant milieu colonization may strengthen our grasp cancer progression and contribute development therapeutic strategies. We report use an engineered model bone marrow (eBM) identify microenvironmental cues regulating cell proliferation investigate how triple-negative breast (TNBC) lines influence hematopoiesis. Notably, individual stromal components niche (osteoblasts, endothelial cells, mesenchymal stem/stromal cells) were each critical quiescence three-dimensional eBM niche. found that hematopoietic stem progenitor cells (HSPCs) impacted TNBC growth responded presence with a shift HSPCs (CD34 + CD38 − ) downstream myeloid lineages (CD11b CD14 ). To account heterogeneity show proof-of-concept ability patient-specific studies, we demonstrate patient-derived organoids survive proliferate eBM, resulting distinct shifts myelopoiesis are similar those observed aggressively lines. envision this will facilitate studies niche-specific individualized responses treatment.

Язык: Английский

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Recent Advances in Hydrogel-Based 3D Disease Modeling and Drug Screening Platforms

Advances in experimental medicine and biology, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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Bioengineering facets of the tumor microenvironment in 3D tumor models: insights into cellular, biophysical and biochemical interactions

FEBS Open Bio, Год журнала: 2025, Номер unknown

Опубликована: Март 27, 2025

The hallmarks of cancer extend beyond genetic anomalies to encompass a sophisticated tumor microenvironment, involving interactions between and non-cancer cells within dynamic biophysical setting, influencing progression. microenvironment is multifaceted, it increasingly clear that the interaction interdependence these different facets need be better understood. Tissue engineering 3D in vitro models provides an opportunity study their on Cancer metastasis still poses major challenge, accounting for 90% cancer-related deaths. This accentuates critical establish patient-specific model systems replicate complexity at all stages Herein, we outline latest advancements tools utilized analyze such models. Henceforth, multifaceted can elucidated using tools.

Язык: Английский

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Construction and application of liver cancer models in vitro

Engineered Regeneration, Год журнала: 2022, Номер 3(3), С. 310 - 322

Опубликована: Июль 27, 2022

Primary liver cancer is the fifth most common malignancy and third leading cause of death worldwide. Although current advances in treatment cancer, prognosis this remains unfavorable. Appropriate model vitro an important way to study pathogenesis drug screening cancer. This review provides a comprehensive summary discussion on construction application models vitro, particular hepatocellular carcinoma (HCC). Specifically, after introducing methods or techniques for preparing 3D models, summarizes relevant applications these e.g. screening, personalized medicine, other applications. In end, discusses advantages disadvantages proposes future prospects research directions.

Язык: Английский

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A Human Ovarian Tumor & Liver Organ-on-Chip for Simultaneous and More Predictive Toxo-Efficacy Assays

Bioengineering, Год журнала: 2023, Номер 10(2), С. 270 - 270

Опубликована: Фев. 18, 2023

In oncology, the poor success rate of clinical trials is becoming increasingly evident due to weak predictability preclinical assays, which either do not recapitulate complexity human tissues (i.e., in vitro tests) or reveal species-specific outcomes animal testing). Therefore, development novel approaches fundamental for better evaluating anti-cancer treatments. Here, a multicompartmental organ-on-chip (OOC) platform was adopted fluidically connect 3D ovarian cancer hepatic cellular models and resemble systemic cisplatin administration contemporarily investigating drug efficacy hepatotoxic effects physiological context. Computational fluid dynamics performed impose capillary-like blood flows predict diffusion. After concentration screening using 2D/3D tissue models, cytotoxicity assays were conducted OOC compared with static co-cultures dynamic single-organ models. A linear decay SKOV-3 HepG2 liver cell viability observed increasing concentration. Furthermore, showed higher resistance than 2D model conditions. Most importantly, when therapy, experimental approach combining culture, fluid-dynamic conditions, multi-organ connection displayed most predictive toxicity results, demonstrating that OOC-based are reliable 3Rs alternatives preclinic.

Язык: Английский

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Using machine learning approach for screening metastatic biomarkers in colorectal cancer and predictive modeling with experimental validation

Scientific Reports, Год журнала: 2023, Номер 13(1)

Опубликована: Ноя. 8, 2023

Colorectal cancer (CRC) liver metastasis accounts for the majority of fatalities associated with CRC. Early detection is crucial improving patient outcomes but can be delayed due to a lack symptoms. In this research, we aimed investigate CRC metastasis-related biomarkers by employing machine learning (ML) approach and experimental validation. The gene expression profile patients was obtained using GSE41568 dataset, differentially expressed genes between primary metastatic samples were screened. Subsequently, carried out feature selection identify most relevant DEGs LASSO Penalized-SVM methods. commonly selected these methods further analysis. Finally, validation done through qRT-PCR. 11 P-SVM algorithms, among which seven had prognostic value in colorectal cancer. It found that MMP3 decreases stage IV compared other stages (P < 0.01). Also, level WNT11 observed increase significantly 0.001). also WNT5a, TNFSF11, lower, higher tumors. summary, study has identified set potential ML algorithms. findings research may provide new insights into identifying potentially lay groundwork innovative therapeutic strategies treatment disease.

Язык: Английский

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