Abstract
mRNA
therapeutics
have
significantly
evolved
within
the
life
sciences,
particularly
in
applications
such
as
vaccines,
tumor
immunotherapy,
protein
replacement,
gene
editing,
and
monoclonal
antibody
therapy.
To
fully
realize
potential
of
drugs
mitigate
adverse
effects,
substantial
vector
materials
been
developed
for
delivery
these
pharmaceutical
agents.
Lipid
nanoparticles
(LNPs)
represent
most
clinically
advanced
carriers,
recognized
by
U.S.
Food
Drug
Administration
approved
vaccines
numerous
clinical
trials.
Diverse
therapeutic
necessitate
tailored
design
LNPs.
Herein,
we
outline
principles
LNP
delivery,
focusing
specifically
on
their
effectiveness,
targeting
capabilities,
safety
profiles,
nanoparticle
stability.
Additionally,
present
latest
advancements
mRNA‐LNP
technology.
This
review
aims
to
elucidate
benefits
systems
therapeutics,
providing
insights
into
breakthroughs
innovative
ideas
further
enhancing
advantages.
These
summaries
are
dedicated
promoting
broader
LNP‐mRNA
drugs,
aiming
advance
treatment
serious
diseases
an
effective
safe
manner.
Chemical Reviews,
Год журнала:
2024,
Номер
124(3), С. 929 - 1033
Опубликована: Янв. 29, 2024
RNA-based
therapies
have
catalyzed
a
revolutionary
transformation
in
the
biomedical
landscape,
offering
unprecedented
potential
disease
prevention
and
treatment.
However,
despite
their
remarkable
achievements,
these
encounter
substantial
challenges
including
low
stability,
susceptibility
to
degradation
by
nucleases,
prominent
negative
charge,
thereby
hindering
further
development.
Chemically
modified
platforms
emerged
as
strategic
innovation,
focusing
on
precise
alterations
either
RNA
moieties
or
associated
delivery
vectors.
This
comprehensive
review
delves
into
platforms,
underscoring
significance
augmenting
performance
translational
prospects
of
therapeutics.
It
encompasses
an
in-depth
analysis
various
chemically
that
been
instrumental
propelling
therapeutics
toward
clinical
utility.
Moreover,
scrutinizes
rationale
behind
diverse
chemical
modification
techniques
aiming
at
optimizing
therapeutic
efficacy
molecules,
facilitating
robust
management.
Recent
empirical
studies
corroborating
enhancement
through
modifications
are
highlighted.
Conclusively,
we
offer
profound
insights
transformative
impact
drugs
delineates
prospective
trajectories
for
future
development
integration.
Proceedings of the National Academy of Sciences,
Год журнала:
2024,
Номер
121(13)
Опубликована: Март 21, 2024
The
use
of
lipid
nanoparticles
(LNP)
to
encapsulate
and
deliver
mRNA
has
become
an
important
therapeutic
advance.
In
addition
vaccines,
LNP-mRNA
can
be
used
in
many
other
applications.
For
example,
targeting
the
LNP
with
anti-CD5
antibodies
(CD5/tLNP)
allow
for
efficient
delivery
payloads
T
cells
express
protein.
As
percentage
protein
expressing
induced
by
intravenous
injection
CD5/tLNP
is
relatively
low
(4-20%),
our
goal
was
find
ways
increase
mRNA-induced
translation
efficiency.
We
showed
that
cell
activation
using
anti-CD3
antibody
improved
expression
after
transfection
vitro
but
not
vivo.
health
increased
cytokines,
therefore,
mCherry
as
a
reporter,
we
found
culturing
either
mouse
or
human
cytokine
IL7
significantly
delivered
both
CD4
+
CD8
vitro.
By
pre-treating
mice
systemic
followed
tLNP
administration,
observed
Transcriptomic
analysis
treated
revealed
enhanced
genomic
pathways
associated
translation.
Improved
translational
ability
demonstrated
showing
levels
electroporation
cultured
presence
IL7,
IL2
IL15.
These
data
show
selectively
increases
cells,
this
property
improve
tLNP-delivered
Advanced Drug Delivery Reviews,
Год журнала:
2024,
Номер
208, С. 115291 - 115291
Опубликована: Март 19, 2024
Among
non-viral
vectors,
lipid
nanovectors
are
considered
the
gold
standard
for
delivery
of
RNA
therapeutics.
The
success
nanoparticles
delivery,
with
three
products
approved
human
use,
has
stimulated
further
investigation
into
therapeutics
different
pathologies.
This
requires
decoding
pathological
intracellular
processes
and
tailoring
system
to
target
tissue
cells.
complexity
morphology
originates
from
assembling
lipidic
components,
which
can
be
elicited
by
various
methods
able
drive
formation
desired
organization.
In
other
cases,
pre-formed
mixed
induce
self-assembly
structural
reorganization
RNA-loaded
nanoparticles.
this
review,
most
relevant
their
potentialities
described
on
basis
mechanism
particle
architecture.
ACS Nano,
Год журнала:
2024,
Номер
18(26), С. 17267 - 17281
Опубликована: Июнь 13, 2024
Intrinsic
or
acquired
resistance
to
chemical
drugs
severely
limits
their
therapeutic
efficacy
in
cancer
treatment.
Various
intracellular
antioxidant
molecules,
particularly
glutathione
(GSH),
play
a
crucial
role
maintaining
redox
homeostasis
by
mitigating
the
overproduced
reactive
oxygen
species
(ROS)
due
rapid
cell
proliferation.
Notably,
these
antioxidants
also
eliminate
chemical-drug-induced
ROS,
eventually
diminishing
cytotoxicity
and
rendering
them
less
effective.
In
this
study,
we
combined
erastin,
GSH
biosynthesis
inhibitor,
with
2'-deoxy-5-fluorouridine
5'-monophosphate
sodium
salt
(FdUMP),
an
ROS-based
drug,
effectively
disrupt
reverse
chemotherapy
resistance.
Therefore,
efficient
ferroptosis
apoptosis
were
simultaneously
induced
for
enhanced
antitumor
effects.
Additionally,
employed
small
interfering
RNA
targeting
PD-L1
(siPD-L1)
as
third
agent
block
immune-checkpoint
recognition
CD8
CNS Neuroscience & Therapeutics,
Год журнала:
2024,
Номер
30(5)
Опубликована: Май 1, 2024
Abstract
Gliomas
are
the
most
common
primary
tumors
of
central
nervous
system,
with
glioblastoma
multiforme
(GBM)
having
highest
incidence,
and
their
therapeutic
efficacy
depends
primarily
on
extent
surgical
resection
postoperative
chemotherapy.
The
role
intracranial
blood–brain
barrier
occurrence
drug‐resistant
gene
O6‐methylguanine‐DNA
methyltransferase
have
greatly
limited
chemotherapeutic
agents
in
patients
GBM
made
it
difficult
to
achieve
expected
clinical
response.
In
recent
years,
rapid
development
nanotechnology
has
brought
new
hope
for
treatment
tumors.
Nanoparticles
(NPs)
shown
great
potential
tumor
therapy
due
unique
properties
such
as
light,
heat,
electromagnetic
effects,
passive
targeting.
Furthermore,
NPs
can
effectively
load
drugs,
significantly
reduce
side
effects
improve
efficacy,
showing
chemotherapy
glioma.
this
article,
we
reviewed
mechanisms
glioma
drug
resistance,
physicochemical
NPs,
advances
resistance.
We
aimed
provide
perspectives
Heliyon,
Год журнала:
2024,
Номер
10(3), С. e25477 - e25477
Опубликована: Фев. 1, 2024
The
health
of
the
reproductive
system
is
intricately
linked
to
female
fertility
and
quality
life.
There
has
been
a
growing
prevalence
disorders
among
women,
particularly
in
younger
age
groups,
resulting
significant
adverse
effects
on
their
health.
Consequently,
there
an
urgent
need
for
effective
treatment
modalities.
Nanotechnology,
as
advanced
discipline,
provides
innovative
avenues
managing
treating
diseases
by
enabling
precise
manipulation
regulation
biological
molecules
cells.
By
utilizing
nanodelivery
systems,
drugs
can
be
administered
with
pinpoint
accuracy,
leading
reduced
side
improved
therapeutic
efficacy.
Moreover,
nanomaterial
imaging
techniques
enhance
diagnostic
precision
sensitivity,
aiding
assessment
disease
severity
progression.
Furthermore,
implementation
nanobiosensors
facilitates
early
detection
prevention
ailments.
This
comprehensive
review
aims
summarize
recent
applications
nanotechnology
diseases.
latest
advancements
drug
delivery,
diagnosis,
approaches
will
discussed,
emphasis
potential
improve
outcomes
overall
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Март 6, 2024
The
rapid
evolution
of
gene
editing
technology
has
markedly
improved
the
outlook
for
treating
genetic
diseases.
Base
editing,
recognized
as
an
exceptionally
precise
modification
tool,
is
emerging
a
focus
in
realm
disease
therapy.
We
provide
comprehensive
overview
fundamental
principles
and
delivery
methods
cytosine
base
editors
(CBE),
adenine
(ABE),
RNA
editors,
with
particular
on
their
applications
recent
research
advances
treatment
have
also
explored
potential
challenges
faced
by
treatment,
including
aspects
such
targeting
specificity,
safety,
efficacy,
enumerated
series
possible
solutions
to
propel
clinical
translation
technology.
In
conclusion,
this
article
not
only
underscores
present
state
but
envisions
its
tremendous
future,
providing
novel
perspective
It
vast
medicine,
support
progression
medicine
development
innovative
approaches
