Pharmaceutics,
Год журнала:
2024,
Номер
16(12), С. 1487 - 1487
Опубликована: Ноя. 21, 2024
Background:
Skin
inflammation
represents
a
hallmark
of
many
skin
conditions,
from
psoriasis
to
eczema.
Here,
we
present
novel
microemulsion
formulation
for
delivering
low
dose
potent
immunosuppressant,
tacrolimus,
the
local
control.
The
efficacy
topically
delivered
tacrolimus
in
controlling
can
be
enhanced
by
packaging
it
into
microemulsions.
Microemulsions
are
small-size,
thermodynamically
stable,
and
surfactant-rich
emulsions
that
enhance
tissue
penetration
retention
poorly
soluble
drugs,
which
reduce
dosing
frequency
potentially
improve
patient
compliance.
Methods:
We
approach
manufacturing
uses
combination
both
high-energy
methods.
composition
parameters
were
optimized
adopting
Quality
Design
methodologies.
FMECA
(Failure,
Mode,
Effects,
Criticality
Analysis)-based
risk
assessment,
D-optimal
Experiment
(DoE),
statistical
analysis
impacting
responses
through
multiple
linear
regression
(MLR)
was
implemented
identifying
critical
process
parameters.
Results:
Through
QbD
strategy,
stable
with
drug
loading
met
all
quality
attributes
(CQAs)
identified.
optimal
candidate
successfully
scaled
up
three-fold
retained
CQAs.
presented
showed
slow
extended
release
profile
vitro.
Conclusions:
Presented
findings
suggest
microemulsions
promising
delivery
skin.
Further,
also
demonstrated
low-energy
emulsification
microfluidization
processes
produce
robust
small
droplet
size
anti-inflammatory
drugs.
To
best
our
knowledge,
this
is
first
report
QbD-driven
optimization
microfluidization.
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Май 13, 2024
CD4
+
CD25
Foxp3
regulatory
T
cells
(Tregs),
a
vital
component
of
the
immune
system,
are
responsible
for
maintaining
homeostasis
and
preventing
excessive
responses.
This
review
explores
signaling
pathways
cytokines
that
regulate
Treg
cells,
including
transforming
growth
factor
beta
(TGF-β),
interleukin
(IL)-2,
IL-10,
IL-35,
which
foster
differentiation
enhance
immunosuppressive
capabilities
Tregs.
It
also
examines
how,
conversely,
signals
mediated
by
IL-6
tumor
necrosis
-alpha
(TNF-α)
can
undermine
suppressive
functions
or
even
drive
their
reprogramming
into
effector
cells.
The
B7
family
comprises
indispensable
co-stimulators
cell
activation.
Among
its
members,
this
focuses
on
capacity
CTLA-4
PD-1
to
differentiation,
function,
survival
As
Tregs
play
an
essential
role
in
homeostasis,
dysfunction
contributes
pathogenesis
autoimmune
diseases.
delves
potential
employing
Treg-based
immunotherapy
treatment
diseases,
transplant
rejection,
cancer.
By
shedding
light
these
topics,
article
aims
our
understanding
regulation
therapeutic
various
pathological
conditions.
Arabian Journal of Chemistry,
Год журнала:
2024,
Номер
17(10), С. 105968 - 105968
Опубликована: Авг. 17, 2024
Hydrogels
are
three-dimensional
structures
that
serve
as
substitutes
for
the
extracellular
matrix
(ECM)
and
possess
outstanding
physicochemical
biochemical
characteristics.
They
gaining
importance
in
regenerative
medicine
because
of
their
similarity
to
natural
terms
moisture
content
wound
tissue
healing
permeability.
Tissue
engineering
advancements
have
resulted
development
flexible
hydrogels
mimic
dynamic
characteristics
ECM.
Several
approaches
been
applied
produce
from
biopolymers
with
enhanced
functional
structural
different
applications
(TERM).
This
review
provides
a
comprehensive
overview
hydrogel
healing,
engineering,
drug
delivery
systems.
We
outline
types
based
on
physical
chemical
crosslinking,
fundamental
properties,
TERM.
article
provided
recent
literature
within
five
years.
Recent
developments
biopolymer-based
state-of-the-art
discussed,
emphasizing
significant
challenges
future
perspectives.
Acta Biomaterialia,
Год журнала:
2024,
Номер
181, С. 46 - 66
Опубликована: Апрель 30, 2024
Skeletal
muscle
is
a
pro-regenerative
tissue,
that
utilizes
tissue-resident
stem
cell
system
to
effect
repair
upon
injury.
Despite
the
demonstrated
efficiency
of
this
in
restoring
mass
after
many
acute
injuries,
conditions
severe
trauma
such
as
those
evident
volumetric
loss
(VML)
(>20%
by
mass),
self-repair
capability
unable
restore
tissue
architecture,
requiring
interventions
which
currently
are
largely
surgical.
As
possible
alternative,
generation
artificial
using
engineering
approaches
may
also
be
importance
treatment
VML
and
diseases
dystrophies.
Three-dimensional
(3D)
bioprinting
has
been
identified
promising
technique
for
regeneration
complex
architecture
skeletal
muscle.
This
review
discusses
existing
strategies
following
damage,
recent
progress
techniques,
bioinks
used
regeneration,
immunogenicity
scaffold
materials,
vitro
vivo
maturation
techniques
3D
bio-printed
constructs.
The
pros
cons
these
bioink
formulations
highlighted.
Finally,
we
present
current
limitations
challenges
field
critical
factors
consider
become
more
translational
produce
clinically
relevant
engineered
physiopathology
injuries
clinical
types
have
applied
muscle,
commonly
regeneration.
We
discussion
gaps
literature
translation
provide
insights
into
what
believe
will
next
steps
required
before
realization
application
tissue-engineered
humans.
manuscript
an
insightful,
timely,
instructive
guide
future
research
from
fundamental
construct
creation
approach,
down
pathway
achieve
desired
impact
clinic.
Materials Today Bio,
Год журнала:
2024,
Номер
29, С. 101342 - 101342
Опубликована: Ноя. 14, 2024
Hydrogels,
that
are
crosslinked
polymer
networks,
can
absorb
huge
quantities
of
water
and/or
biological
fluids.
Their
physical
properties,
such
as
elasticity
and
soft
tissue,
together
with
their
biocompatibility
biodegradability,
closely
resemble
living
tissues.
The
versatility
hydrogels
has
fuelled
application
in
various
fields,
agriculture,
biomaterials,
the
food
industry,
drug
delivery,
tissue
engineering,
regenerative
medicine.
combination
nanoparticles,
specifically
Mesoporous
Silica
Nanoparticles
(MSNs),
have
elevated
these
composites
to
next
level,
since
MSNs
could
improve
hydrogel
mechanical
ability
encapsulate
controlled
release
great
amounts
different
therapeutic
agents,
responsiveness
a
variety
external
internal
stimuli.
In
this
review,
main
features
both
introduced,
followed
by
discussion
hydrogels-MSNs
structures
an
overview
use
applications,
delivery
technologies
engineering.
Biomaterials Research,
Год журнала:
2025,
Номер
29
Опубликована: Янв. 1, 2025
Exosomes
(Exos)
are
tiny
extracellular
vesicles
containing
a
variety
of
active
biomolecules
that
play
important
parts
in
intercellular
communication
and
influence
the
functions
target
cells.
The
potential
Exos
treatment
dermatological
diseases
has
recently
been
well
appreciated.
This
review
highlights
constituents,
function,
delivery
Exos,
with
particular
focus
on
their
applications
skin
therapy.
Firstly,
we
offer
concise
overview
biochemical
properties
including
sources,
structures,
internal
constituents.
Subsequently,
biomedical
latest
advances
extraction
purification
summarized.
We
further
discuss
modes
underscore
biomaterials
this
regard.
Finally,
summarize
application
Exo-aided
therapy
dermatology.
Overall,
objective
is
to
provide
comprehensive
perspective
recent
advancements
Exo-based
approaches
treating
diseases,
intention
guiding
future
research
efforts.
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(8), С. 3874 - 3874
Опубликована: Апрель 19, 2025
Spinal
cord
injury
(SCI)
remains
a
major
clinical
challenge,
with
limited
therapeutic
options
for
restoring
lost
neurological
function.
While
efforts
to
mitigate
secondary
damage
have
improved
early-phase
management,
achieving
sustained
neurorepair
and
functional
recovery
elusive.
Advances
in
stem
cell
engineering
regenerative
medicine
opened
new
avenues
targeted
interventions,
particularly
through
the
transplantation
of
neural
stem/progenitor
cells
(NSPCs),
induced
pluripotent
(iPSCs),
mesenchymal
(MSCs).
However,
patient-specific
factors
such
as
cellular
senescence,
genetic
epigenetic
variability,
microenvironment,
comorbidities
influence
efficacy
therapies
by
affecting
graft
survival
differentiation.
Overcoming
these
challenges
necessitates
cutting-edge
technologies,
including
single-cell
transcriptomics,
CRISPR-mediated
hypoimmunogenic
engineering,
biomaterial-based
delivery
platforms,
which
enable
personalized
precision-driven
SCI
repair.
Leveraging
advancements
may
help
overcome
translational
barriers
establish
clinically
viable
solutions.
This
review
explores
intersection
bioengineering
innovations,
transcriptomic-guided
precision
define
next
frontier
therapy.
Frontiers in Immunology,
Год журнала:
2025,
Номер
16
Опубликована: Апрель 22, 2025
Metabolic
syndrome
(MetS)
is
a
group
of
symptoms
that
are
characterized
by
abnormal
changes
in
metabolic
substances
such
as
glucose,
lipids,
proteins,
and
bile
acids.
MetS
common
complication
after
organ
transplantation
can
further
affect
the
survival
physiological
function
graft
reprograming
patient's
immune
environment.
Additionally,
influence
occurrence
post-transplant
complications,
infections.
In
recent
years,
research
into
epidemiology
mechanisms
has
grown
significantly.
this
review,
we
summarize
hyperglycemia,
insulin
resistance,
hyperlipidemia,
acids,
amino
acids
on
body's
cells
related
to
effect
disorders
rejection
liver,
kidney,
heart,
skin
other
transplantation.
Finally,
provide
an
overview
current
treatment
strategies
offer
insights
potential
future
therapies
for
managing
transplant
recipients.
