Experimental Eye Research, Год журнала: 2022, Номер 219, С. 109035 - 109035
Опубликована: Март 17, 2022
Язык: Английский
The Ocular Surface, Год журнала: 2023, Номер 30, С. 119 - 128
Опубликована: Авг. 26, 2023
Язык: Английский
Процитировано
13
Cells, Год журнала: 2023, Номер 12(10), С. 1435 - 1435
Опубликована: Май 21, 2023
The lacrimal gland (LG) secretes aqueous tears. Previous studies have provided insights into the cell lineage relationships during tissue morphogenesis. However, little is known about types composing adult LG and their progenitors. Using scRNAseq, we established first comprehensive atlas of mouse to investigate hierarchy, its secretory repertoire, sex differences. Our analysis uncovered complexity stromal landscape. Epithelium subclustering revealed myoepithelial cells, acinar subsets, two novel subpopulations: Tfrchi Car6hi cells. ductal compartment contained Wfdc2+ multilayered ducts an Ltf+ cluster formed by luminal intercalated duct Kit+ progenitors were identified as: Krt14+ basal Aldh1a1+ cells ducts, Sox10+ epithelial clusters. Lineage tracing experiments that populations contribute myoepithelial, acinar, lineages. scRNAseq data, found postnatally developing epithelium harbored key features putative Finally, showed produce most sex-biased lipocalins secretoglobins detected in study provides a wealth new data on maintenance identifies cellular origin tear components.
Язык: Английский
Процитировано
12
Investigative Ophthalmology & Visual Science, Год журнала: 2025, Номер 66(1), С. 12 - 12
Опубликована: Янв. 7, 2025
Purpose: Chronic jet lag (CJL) is known to disrupt circadian rhythms, which regulate various physiological processes, including ocular surface homeostasis. However, the specific effects of CJL on lacrimal gland function and underlying cellular mechanisms remain poorly understood. Methods: A model was established using C57BL/6J mice. Extraorbital glands (ELGs) were collected at 3-hour intervals for RNA extraction high-throughput sequencing. Circadian transcriptomic profiles analyzed, functional annotations performed. Hydrogen peroxide levels total antioxidant capacity in tear fluid measured chemometric assays. Immunofluorescence used assess cell proliferation, apoptosis, immune infiltration ELGs, reactive oxygen species (ROS) accumulation. The potential therapeutic alpha-lipoic acid (ALA) CJL-induced oxidative stress pathological changes ELGs also investigated. Results: significantly disrupted locomotor activity, altered body temperature modified diurnal oscillations ELGs. Transcriptomic analysis revealed extensive rhythmic gene expression, phase shifts, pathway clustering response CJL. disruption core clock transcription associated with ELG hyperproliferation increased ROS tert-Butyl hydroperoxide promoted ALA effectively reduced mitigated hyperproliferation. Conclusions: These findings uncover novel molecular pathways affected by highlight therapies, such as ALA, preserving health under conditions rhythm disruption.
Язык: Английский
Процитировано
0
GeroScience, Год журнала: 2025, Номер unknown
Опубликована: Март 7, 2025
Язык: Английский
Процитировано
0
Investigative Ophthalmology & Visual Science, Год журнала: 2022, Номер 63(6), С. 19 - 19
Опубликована: Июнь 22, 2022
Sleep loss markedly affects the structure and function of lacrimal gland may cause ocular surface disease as a common public health problem. This study aims to investigate circadian disturbance caused by sleep leading dysfunction extraorbital glands (ELGs).A mouse deprivation (SD) model for studies was built in C57BL/6J male mice. After four weeks, ELGs were collected at three-hour intervals during 24-hour period. The Jonckheere-Terpstra-Kendall algorithm used determine composition, phase, rhythmicity transcriptomic profiles ELGs. Furthermore, we compared non-sleep-deprived SD-treated ELG (i) reactive oxygen species (ROS) fluorescein staining, (ii) DNA damage immunostaining γ-H2Ax, (iii) migration immune cells CD4, CD8, γδ-TCR, CD64, CX3CR1. Finally, also evaluated locomotor activity core body temperature rhythm mice mass, cell size, tear secretion ELGs.SD dramatically altered composition phase-associated functional enrichment transcriptome, trafficking, metabolism, differentiation, neural secretory activities Additionally, SD ROS accumulation consequent ELGs, irreversible.SD damages structure, function, diurnal oscillations These results highlight comprehensive characterization insufficient sleep-affected transcriptome that provide new therapeutic approach counteract effects on function.
Язык: Английский
Процитировано
17
The Ocular Surface, Год журнала: 2024, Номер 33, С. 64 - 73
Опубликована: Май 4, 2024
Язык: Английский
Процитировано
3
Frontiers in Nutrition, Год журнала: 2022, Номер 8
Опубликована: Янв. 13, 2022
Objectives: To evaluate the effect of astaxanthin in treatment mild-to-moderate dry eye disease (DED) middle-aged and elderly patients. Methods: 120 eyes 60 patients with DED were enrolled this prospective, one-group, quasi-experimental study. Six milligram Astaxanthin tablets (Weihong Haematococcus Pluvialis Astaxanthin, Hangzhou Xinwei Low Carbon Technology R&D Co., Ltd., China) administered orally, twice daily for 30 ± 2 days. History diseases, treatment, systemic disease, medication before test recorded. In addition, ocular surface index (OSDI) questionnaire, non-invasive tear break-up time (NIBUT), fluorescein (FBUT), corneal staining (CFS) score, eyelid margin signs, meibomian gland (MG) expressibility, meibum quality, dropout (MGDR), Schirmer I (SIt), meniscus height (TMH), bulbar conjunctiva congestion degree, blink frequency, incomplete rate, thickness film lipid layer collected weeks after initiation at end treatment. Visual acuity (VA), intraocular pressure (IOP), anterior segment, fundus, discomfort symptoms other adverse reactions also monitored throughout study to assess safety. Results: OSDI NIBUT, BUT, CFS MG frequency improved significantly varying degrees compared those (P < 0.05), while TMH, SIt, conjunctival congestion, layer, MGDR, VA IOP did not differ > 0.05). Conclusions: Oral administration improves signs DED.
Язык: Английский
Процитировано
15
GeroScience, Год журнала: 2022, Номер 44(4), С. 2105 - 2128
Опубликована: Март 12, 2022
Язык: Английский
Процитировано
15
Mechanisms of Ageing and Development, Год журнала: 2023, Номер 215, С. 111869 - 111869
Опубликована: Сен. 9, 2023
Iron is the most abundant trace element in human body. Since iron can switch between its 2-valent and 3-valent form it essential various physiological processes such as energy production, proliferation or DNA synthesis. Especially high metabolic organs heart rely on iron-associated iron-sulfur heme proteins. However, due to switches oxidation state, overload exhibits toxicity through formation of reactive oxygen species, underlining importance balanced levels. Growing evidence demonstrates disturbance this balance during aging. While age-associated cardiovascular diseases are often related deficiency, aging cardiac accumulates. To understand these changes, we focused inflammation proteolysis, two hallmarks aging, their role metabolism. Via IL-6-hepcidin axis, status strongly connected resulting anemia accompanied by infiltration macrophages. This tight connection highlights macrophage metabolism inflammation. Age-related decrease proteolytic activity additionally affects impaired degradation Therefore, review accentuates alterations with regards proteolysis draw attention implications associations.
Язык: Английский
Процитировано
8
Immunology, Год журнала: 2021, Номер 164(1), С. 43 - 56
Опубликована: Апрель 10, 2021
The ocular surface is the part of visual system directly exposed to environment, and it comprises cornea, first refractive tissue layer its surrounding structures. has evolved keep cornea smooth wet, a prerequisite for proper sight, also protected. To this aim, bona fide mucosal niche with an immune capable fighting against dangerous pathogens. However, due potential harmful effects uncontrolled inflammation, several mechanisms response in check. Specifically, maintained inflammation-free functional by particular form peripheral tolerance known as tolerance, markedly different from privilege intraocular Remarkably, conjunctival akin oral respiratory found gut airways, respectively. And similarly, immunoregulation eye affected ageing just digestive tracts. With comes increased prevalence immune-based disorders, which could be related age-related impairment tolerance. purpose review was summarize present knowledge how process light current literature on airways.
Язык: Английский
Процитировано
20