Screening asthmatics for atopic status using the ALergy EXplorer (ALEX ² ) macroarray

Journal of Asthma, Год журнала: 2024, Номер 61(9), С. 1050 - 1057

Опубликована: Фев. 28, 2024

Screening asthma patients for atopy facilitates management. Since 2010, the core biomarker screening subjects atopic status has been qualitative Phadiatop. multi-aeroallergen screen. A more quantitative macroarray, Allergy Explorer (ALEX

Язык: Английский

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Molecular allergen sensitization drives phenotypes of severe asthma in children: Evidence from a megacity cohort (SAMP)

Pediatric Allergy and Immunology, Год журнала: 2024, Номер 35(12)

Опубликована: Дек. 1, 2024

Abstract Background Several major sensitization profiles have been described in children with asthma, but it remains unclear how these relate to asthma phenotypes. The aim of this study was determine allergenic a megacity cohort (SAMP). Methods This cross‐sectional analysis performed from 2011 2015 including preschool and school‐age severe moderate the SAMP cohort. We ALEX multiplex array carried out cluster analysis. Results Data 367 were analyzed: 224 age 143 school age, respectively 84 (38%) 114 (80%) presented at least one allergic sensitization. At three clusters identified: Cluster 1, Few sensitizations inhaled allergen molecular families non‐type 2 (T2) inflammation ( n = 61); 2, Predominant HDM 16); 3, Severe multiple food 7). five non‐T2 43); 31); PR‐10 protein family 25); 4, predominant tropomyosin 11); 5, 4). Conclusion These results underline heterogeneity childhood asthma. most phenotypes associated both as expected, family, novel finding.

Язык: Английский

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Molecular peanut sensitization patterns in Lithuanian children with suspected allergic symptoms

Advances in Dermatology and Allergology, Год журнала: 2023, Номер 40(1), С. 107 - 110

Опубликована: Янв. 1, 2023

There are no in-depth studies describing the peanut sensitization molecular patterns in Lithuanian children.To investigate age-related of profiles children with suspected allergic symptoms.We performed a retrospective analysis 576 possible symptoms. Patient data were categorized according to age groups: 0-2, 3-6, 7-12, and 13-18 years. Specific immunoglobulin E levels components: Ara h 1, 2, 3, 6, 8, 9, 15, birch major allergen component Bet v 1 analyzed.Sensitization at least one protein was observed 148 (25.7%) children. In aged ≤ 2 years, most sensitized - 11 (12.1%). from 3 6 remained prevalent 40 (16.6%) The 7 12 years 8 39 (24.5%) them. ≥ 13 sensitizer 21 (24.7%) them.One-fourth symptoms Lithuania. Infants preschool commonly seed storage proteins, while school-age allergen.

Язык: Английский

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Diagnostic accuracy of the ALEX² test in peanut‐sensitized children

Clinical & Experimental Allergy, Год журнала: 2023, Номер 53(10), С. 1041 - 1044

Опубликована: Май 29, 2023

To the editor, The fundamental concept when diagnosing allergies is that detection of immunoglobulin E (IgE) sensitization and presence allergy are not identical. Complementary to conventional testing based on allergen extracts, molecular diagnosis reduces need for oral food challenges (OFCs) in diagnosis. detect IgE specific molecules, different singleplex or multiplex tests available. Multiplex consume less serum costly a higher number measurements required, they reveal reactivity against broad panel one test run. Currently, there two commercial microarrays available clinical use, is, ISAC113 (Thermo Fisher, Uppsala, Sweden) ALEX2 (Macro Array Diagnostics, Vienna, Austria) which showed comparable sensitivity specificity previous studies.1, 2 Here, we aimed investigate diagnostic accuracy Turkish children with peanut compared serological skin as reference. This retrospective study, reported line current STARD guideline, included 123 who had been referred Hacettepe University Paediatric Allergy Department due suspected workup. Peanut confirmed either by prick (SPT) and/or extract-specific (ImmunoCAP, Thermo Fisher). For all sera, data sIgE components, detected test, were also (Figure 1). While 31 (25%) clinically reactive peanut, 92 (75%) tolerant. Clinical was assessed OFC (n = 8) convincing history 23); (see supplementary Appendix Table S1 additional material, https://zenodo.org/record/7836201#.ZD0d6exBz9E). details study population, design, SPT, sIgE, material. protocol reviewed approved Institutional Review Board (GO-21/1248). There significant differences between peanut-reactive peanut-tolerant groups SPT wheal size, extract total Ara h 1, 2, 3 6 material). Based recommended cut-off level ≥0.3 kUA/L 83.9% (26/31) 77.4% (24/31) group, but only 15.2% (14/92) 10.9% (10/92) tolerant respectively (p < .001 both). 1 3, corresponding values group 96.8% (30/31) 90.3% (28/31), 70.7% (65/92) 37.0% (34/92), .002 p .001). In if consider 2S albumins (Ara 6), proportion IgE-reactivity 6, both neither 9.7% (3/31), 3.2% (1/31), 74.2% (23/31) 12.9% (4/31), respectively. Those four patients (12.9%) sensitized nor 3. 8 patients, whom 9 48.4% (15/31) 38.0% (35/92) .332). Hierarchical cluster analysis pairwise association well 1B). ROC curve analysis, (AUC 0.927), 0.854), 0.829), 0.882), 0.857) 0.853) similar potential diagnose all) 1C). Comparison AUCs revealed no difference extract-sIgE components S2 Using threshold levels (≥0.3 ALEX2, ≥3 mm diameter ≥0.35 kU/L ImmunoCAP), highest accuracies achieved (84.6% 86.2%, respectively), 59.4% 30.3% (Table optimal extract-sIgE, according Youden index, 6.5 mm, 21.4 kUA/L, 6.21, 0.84, 0.92 0.16 By combining tests, criteria more stringent could improve accuracy. When applying levels, combination 80.0% sensitivity, 92.2% specificity, 89.2% 10.3 likelihood ratio (LR+) 76.7%, 94.4%, 90.0% 13.7, S3 Overall, combinations + (90.9%) 1D). However, former (SPT 6) better LR+ than latter (22.2 vs 3.6, respectively). observation our cohort AUC other may be explained fact found this studies.3, 4 present although best predict rates peanut-allergic rarely previously.5, Variations prevalence potentially linked geographical locations, particularities respective populations, amount/ way how peanuts consumed (roasted nut versus cooked forms) assay. 0.3 84.8%. using (0.83 kUA/L), high maintained, while slightly increased 89.1%. Similarly, ISAC IgE-microarray, value 0.81 ISU 86% 82%, respectively.7 results accordance recently published systematic review paediatric studies where 0.35 (ImmunoCAP) have 85.8% (95% CI 79.3–90.6) 83.6% 76.0–89.2).8 We (22.2). Recently, Hemmings major (0.94) ISAC.9 analysis. We, therefore, conclude highly sensitive approach peanuts. Analysing individual patterns allergens belonging albumin family, rate co-sensitization (74.2%) and, reports, monosensitization observed (3.2%). It previously shown (Bet v homologue) frequent from regions, birch common. Low probably low frequency region. Moreover, cohort, (nsLTP) suitable distinguish IgE-reactivities (48.4% vs. 38.0%). do contribute young population. Limitations relatively small done few participants, mainly attributable COVID-19 restrictions consecutive decline hospital admissions. obvious clinical, demographic laboratory findings history. A final limitation nature physicians categorized blinded access data. summary, provide confirmatory evidence IgE-antibodies measured predictors children. particular, provided accuracy, each parameter alone. real-life population Eastern Mediterranean region, show first time complementary allergy. ESA contributed acquisition data, interpretation original draft writing. OS UMS statistical plan performed CL SW language editing. BES concept, revising critically important intellectual content. All authors manuscript version. thank department staff peanut-specific measurements. Elif Soyak Aytekin, Ozge Soyer, Umit Murat Sahiner Bulent Enis Sekerel conflict interest. Sandra Wieser Christian Lupinek employees MADx Diagnostics). No funding received study. support request author. publicly privacy ethical restrictions. S1. Please note: publisher responsible content functionality any supporting information supplied authors. Any queries (other missing content) should directed author article.

Язык: Английский

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Component-resolved Diagnostics in Allergy Practice Focusing on Food Allergy: A Systematic Review

Bengal Physician Journal, Год журнала: 2023, Номер 10(2), С. 29 - 42

Опубликована: Авг. 4, 2023

In this systematic review, we have discussed the role of component-resolved diagnostics (CRD) in diagnosis immunoglobulin E (IgE)mediated food allergies; IgE-mediated allergies are adverse reactions to caused by an immunologic mechanism involving specific IgE (sIgE) antibodies.While self-reported common, prevalence confirmed through oral challenge (OFC) is estimated be around 1%.The conventional diagnostic process for involves clinical history, vivo and/or vitro tests, and OFC.However, many components used these tests irrelevant process.Component-resolved a technique that uses purified allergens detect sIgE antibody responses against individual allergenic molecules.It aims enhance specificity testing differentiate between genuine sensitization cross-reactivity-induced sensitization.Component-resolved also helps stratifying risk associated with patterns predicting OFC outcomes.However, CRD cannot replace as gold standard due insufficient sensitivity levels.Proper interpretation crucial avoid unnecessary elimination diets auto-injector prescriptions may impact patients' quality life.Component-resolved plays significant work-up identifying characterizing compounds causing allergic responses.It enables differentiation primary secondary sensitization, predicts disease progression risk, aids results.However, there gaps research practice.Commercial only available limited number allergens, costly compared other it lacks sufficient OFC.Further initiatives necessary address improve use allergy diagnosis.

Язык: Английский

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Molecular diagnosis contribution for personalized medicine

Current Opinion in Allergy and Clinical Immunology, Год журнала: 2022, Номер 22(3), С. 175 - 180

Опубликована: Фев. 16, 2022

Purpose of review The purpose the current is to highlight most recent findings in molecular allergy and its applicability precision medicine for allergic patients. Recent Molecular provides useful information areas respiratory (house dust mites, pet dander pollen allergy), food (tree nuts, peanuts, fruits vegetables), hymenoptera venom others, order improve management Regional differences sensitization profiles, assay characteristics interpretation relation whole extracts total immunoglobulin E need be taken into account. Studies impact such strategies are needed. Summary diagnosis represents a major contribution personalized medicine. It aids assesment risk prediction, disease severity, genuine/cross-reactive sensitization, finally apply precise strategies.

Язык: Английский

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Molecular Profiles of Sensitization to Non-Specific Lipid Transfer Proteins in Lithuania: Single Center Experience

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(24), С. 13535 - 13535

Опубликована: Дек. 18, 2024

Non-specific Lipid Transfer proteins (nsLTPs) are relevant allergens of several pollens and plant foods. Sensitization to nsLTPs is not typical in our region. Still, it has become an increasingly common cause IgE-mediated food allergies food-induced anaphylaxis Northern Europe recent decades. No in-depth studies describe the prevalence sensitization molecular components Lithuania. This study aimed determine profile atopic patients at Immunology Allergy Department Kauno Klinikos nsLTPs, using allergen component analysis. Sixty Lithuanian adults with symptoms allergic rhinitis and/or asthma were included into study. Specific immunoglobulin E (IgE) levels measured two vitro techniques: extract Results showed that 25% subjects sensitized nsLTP-containing sources, mostly Zea m 14, Mal d 3, Vit v 1, Art 3. The median amount total IgE was higher nsLTP-sensitized than nsLTP-nonsensitized patients. Based on Cohen’s Kappa McNemar tests, results analysis tests do always agree, especially when we sources containing nsLTPs. Molecular could be first choice determining detailed who experienced unknown origin.

Язык: Английский

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Comparaison des performances diagnostiques de deux biopuces IgE : ISAC® et ALEX2®

Revue française d'allergologie, Год журнала: 2023, Номер 63(2), С. 103289 - 103289

Опубликована: Янв. 25, 2023

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Would the choice of multiplex platform impact the management of the allergic patient? A first approach focusing on LTPs

Journal of Clinical Laboratory Analysis, Год журнала: 2023, Номер 37(15-16)

Опубликована: Авг. 1, 2023

In the Mediterranean area, patients with LTP syndrome who are sensitized to multiple allergens often tested for sIgE using multiplex platforms. The results obtained from different commercial platforms not interchangeable, so it is important compare and validate platform selected use. objective of this study performance ImmunoCAP ISAC E112i macroarray ALEX2 in our daily practice.From August 2021 March 2022, we 20 random serum samples polysensitized test (MADx) tIgE (Thermo Fisher Scientific). We compared total IgE (tIgE) levels shared allergens.The heatmap generally showed more intense ISAC. overall correlation was good, but some exceptions were noted. main discrepancies found Ole e 7, which positive 11 negative all ALEX2, nut LTPs, a threefold higher detection rate Ara h 9 fivefold Cor 8 Jug r 3 ALEX2. regression model no interchangeability results.Despite small sample size complexity comparing quantitative semi-quantitative platform, suggest that patient diagnosis management can be influenced by used. Therefore, findings must taken into consideration when choosing use profiles LTP-polysensitized patients, even though data needed.

Язык: Английский

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Feasibility of the ALEX multiplex platform in the diagnosis of nut allergy from a Mediterranean population

Clinica Chimica Acta, Год журнала: 2022, Номер 535, С. 112 - 119

Опубликована: Авг. 27, 2022

Язык: Английский

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