Pharmaceutics,
Год журнала:
2023,
Номер
15(7), С. 1821 - 1821
Опубликована: Июнь 26, 2023
Cancer
immunotherapy
can
significantly
prevent
tumor
growth
and
metastasis
by
activating
the
autoimmune
system
without
destroying
normal
cells.
Although
cancer
has
made
some
achievements
in
clinical
treatment,
it
is
still
restricted
systemic
immunotoxicity,
immune
cell
dysfunction,
heterogeneity,
immunosuppressive
microenvironment
(ITME).
Biomimetic
cell-derived
nanoparticles
are
attracting
considerable
interest
due
to
their
better
biocompatibility
lower
immunogenicity.
Moreover,
biomimetic
achieve
different
preferred
biological
effects
inherent
abundant
source
cell-relevant
functions.
This
review
summarizes
latest
developments
for
immunotherapy,
discusses
applications
of
each
analyzes
challenges
transformation.
Frontiers in Pharmacology,
Год журнала:
2023,
Номер
14
Опубликована: Фев. 16, 2023
Nanoparticle
drug
delivery
systems
have
proved
anti-tumor
effects;
however,
they
are
not
widely
used
in
tumor
therapy
due
to
insufficient
ability
target
specific
sites,
multidrug
resistance
drugs,
and
the
high
toxicity
of
drugs.
With
development
RNAi
technology,
nucleic
acids
been
delivered
sites
replace
or
correct
defective
genes
knock
down
genes.
Also,
synergistic
therapeutic
effects
can
be
achieved
for
combined
delivery,
which
is
more
effective
overcoming
cancer
cells.
These
combination
therapies
achieve
better
than
delivering
chemotherapeutic
drugs
alone,
so
scope
has
also
expanded
three
aspects:
drug-drug,
drug-gene,
gene-gene.
This
review
summarizes
recent
advances
nanocarriers
co-delivery
agents,
including
i)
characterization
preparation
nanocarriers,
such
as
lipid-based
polymer
inorganic
carriers;
ii)
advantages
disadvantages
approaches;
iii)
effectual
cases
that
applied
systems;
iv)
future
perspectives
design
nanoparticle
co-deliver
agents.
Abstract
Extracellular
vesicles
(EVs)‐based
intercellular
communication
(through
exosomes,
microvesicles,
and
apoptotic
bodies)
is
conserved
across
all
kingdoms
of
life.
In
recent
years,
exosomes
have
gained
much
attention
for
targeted
pharmaceutical
administration
due
to
their
unique
features,
nanoscale
size,
capacity
significantly
contribute
cellular
communication.
As
drug
delivery
vehicles,
several
advantages
over
alternative
nanoparticulate
technologies.
A
key
advantage
lies
in
comparable
makeup
the
body's
cells,
which
makes
them
non‐immunogenic.
However,
face
challenges,
including
a
lack
an
effective
standard
production
technique,
decreased
loading
capacity,
limited
characterization
techniques,
underdeveloped
isolation
purification
procedures.
Exosomes
are
well
known
long‐term
safety
natural
ability
transport
nucleic
acids
medicinal
compounds
blood‐brain‐barrier
(BBB).
Therefore,
addition
revealing
new
insights
into
exosomes’
distinctiveness,
growing
availability
analytical
tools
may
drive
development
next‐generation
synthetic
systems.
Herein,
light
shed
on
as
vehicles
anti‐infective
therapy
by
reviewing
literature
primary
articles
published
between
2002
2023.
Additionally,
benefits
limitations
employing
therapeutic
also
discussed.
Pharmaceutics,
Год журнала:
2023,
Номер
15(8), С. 2070 - 2070
Опубликована: Авг. 1, 2023
Exosomes
are
nanoscale
vesicles
released
by
diverse
types
of
cells
for
complex
intercellular
communication.
Numerous
studies
have
shown
that
exosomes
can
regulate
the
body’s
immune
response
to
tumor
and
interfere
with
microenvironment
(TME).
In
clinical
trials
on
dendritic
cell
(DC)-based
antitumor
vaccines,
no
satisfactory
results
been
achieved.
However,
recent
suggested
DC-derived
(DEXs)
may
be
superior
DC-based
vaccines
in
avoiding
cell-mediated
immunosuppression.
DEXs
contain
multiple
surface
markers
capture
tumor-associated
antigens
(TAAs)
promote
cell-dependent
rejection.
These
findings
indicate
necessity
further
development
improvement
DEX-based
cell-free
complement
chemotherapy,
radiotherapy,
other
immunotherapies.
this
review,
we
highlighted
progress
cancer
immunotherapy,
particularly
concentrating
landmark
biological
characterization
DEXs,
summarized
their
important
role
(TIME)
application
targeted
immunotherapy.
This
review
could
enhance
comprehension
advances
immunotherapy
contribute
elucidation
how
TIME,
thereby
providing
a
reference
utilizing
practice.
Pharmaceutics,
Год журнала:
2023,
Номер
15(3), С. 984 - 984
Опубликована: Март 18, 2023
Clustered
regularly
interspaced
short
palindromic
repeat
(CRISPR)/CRISPR-associated
protein
(Cas)
systems
can
precisely
manipulate
DNA
sequences
to
change
the
characteristics
of
cells
and
organs,
which
has
potential
in
mechanistic
research
on
genes
treatment
diseases.
However,
clinical
applications
are
restricted
by
lack
safe,
targeted
effective
delivery
vectors.
Extracellular
vesicles
(EVs)
an
attractive
platform
for
CRISPR/Cas9.
Compared
with
viral
other
vectors,
EVs
present
several
advantages,
including
safety,
protection,
capacity,
penetrating
ability,
targeting
ability
modification.
Consequently,
profitably
used
deliver
CRISPR/Cas9
vivo.
In
this
review,
advantages
disadvantages
form
vectors
concluded.
The
favorable
traits
as
such
innate
characteristics,
physiological
pathological
functions,
safety
EVs,
summarized.
Furthermore,
terms
EV
sources
isolation
strategies,
loading
methods
have
been
concluded
discussed.
Finally,
review
provides
future
directions
system
applications,
consistent
quality,
yield
EVs.
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(6), С. 3080 - 3080
Опубликована: Март 7, 2024
This
paper
sheds
light
on
the
alarming
issue
of
antibiotic
resistance
(ABR)
in
aquatic
environments,
exploring
its
detrimental
effects
ecosystems
and
public
health.
It
examines
multifaceted
role
use
aquaculture,
agricultural
runoff,
industrial
waste
fostering
development
dissemination
resistant
bacteria.
The
intricate
interplay
between
various
environmental
factors,
horizontal
gene
transfer,
bacterial
extracellular
vesicles
(BEVs)
accelerating
spread
ABR
is
comprehensively
discussed.
Various
BEVs
carrying
genes
like
blaCTX-M,
tetA,
floR,
sul/I,
as
well
their
contribution
to
dominance
multidrug-resistant
bacteria,
are
highlighted.
potential
both
a
threat
tool
combating
explored,
with
promising
strategies
targeted
antimicrobial
delivery
systems
probiotic-derived
EVs
holding
significant
promise.
underscores
urgency
understanding
environments.
By
unraveling
these
unseen
weapons,
we
pave
way
for
developing
effective
mitigate
ABR,
advocating
multidisciplinary
approach
that
includes
stringent
regulations,
enhanced
wastewater
treatment,
adoption
sustainable
practices
aquaculture.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Янв. 3, 2024
Abstract
With
the
immense
progress
in
drug
delivery
systems
(DDS)
and
rise
of
nanotechnology,
challenges
such
as
target
specificity
remain.
The
vesicle-vector
system
(VVS)
is
a
that
uses
lipid-based
vesicles
vectors
for
targeted
delivery.
When
modified
with
target-probing
materials,
these
become
powerful
high
specificity.
In
this
review,
we
discuss
three
general
types
VVS
based
on
different
modification
strategies:
(1)
vesicle-probes;
(2)
vesicle-vesicles;
(3)
genetically
engineered
vesicles.
synthesis
each
type
their
corresponding
properties
are
advantageous
delivery,
also
highlighted.
applications,
challenges,
limitations
briefly
examined.
Finally,
share
number
insights
perspectives
regarding
future
at
nanoscale.
Graphical
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
11
Опубликована: Янв. 19, 2024
Extracellular
vesicles
(EVs)
are
membrane
structures
enclosed
by
a
lipid
bilayer
that
released
into
the
extracellular
space
all
types
of
cells.
EVs
involved
in
many
physiological
processes
transporting
biologically
active
substances.
Interest
for
diagnostic
biomarker
research
and
therapeutic
drug
delivery
applications
has
increased
recent
years.
The
realization
full
potential
is
currently
hampered
lack
suitable
technology
isolation
purification
downstream
pharmaceutical
applications.
Anion
Exchange
Chromatography
(AEX)
an
established
method
which
specific
charges
on
AEX
matrix
can
exploit
surface
their
interactions
to
provide
productive
scalable
separation
method.
using
Eshmuno
®
Q,
strong
tentacle
anion
exchange
resin,
was
used
demonstrate
principal
feasibility
AEX-based
gain
insight
isolated
EV
properties.
Using
several
analysis
techniques
more
detailed
populations
during
isolation,
we
demonstrated
although
composition
CD9/63/81
remained
constant
tetraspanin
positive
EVs,
size
distribution
purity
changed
elution.
Higher
salt
concentrations
eluted
larger
negative
vesicles.
