Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Май 31, 2023
Abstract
Background:
Rosai-Dorfman
disease
(RDD)
is
a
rare
non-Langerhans
cell
histiocytosis
characterized
by
massive
lymphadenopathy
and
systemic
extranodal
lesions.
Case
presentation:
We
present
case
of
28-year-old
female
who
presented
with
recurrent
blurred
vision
in
her
right
eye
for
3
months.
Her
left
had
been
blinded
atrophied
decade
prior.
She
subsequently
developed
headache,
fever,
impaired
mental
status.
Cranial
MRI
indicated
hypertrophic
pachymeningitis
(HP),
18
F-FDG
PET/CT
revealed
significant
FDG
uptake
the
dura
mater.
Autoimmune
testing
elevated
anti-nuclear,
anti-SS-A,
anti-SS-B
antibodies.
Incisional
biopsy
atrophic
eyeball
RDD
marked
polyclonal
plasmacytosis.
The
patient
was
diagnosed
accompanied
multisystem
involvement,
including
Sjögren’s
syndrome
(SS),
panuveitis,
HP.
Treatment
methylprednisolone
over
several
weeks
resulted
improvement.
Conclusions:
This
first
reported
presenting
SS
combination
panuveitis
Although
rarely
young
patients,
interdisciplinary
collaboration
essential
to
prevent
delayed
diagnosis.
ACS Nano,
Год журнала:
2024,
Номер
18(3), С. 2370 - 2383
Опубликована: Янв. 8, 2024
Nanoplastics
(NPs)
pervade
daily
life,
posing
serious
threats
to
marine
ecosystems.
Despite
the
crucial
role
that
surface
charge
plays
in
NP
effects,
there
is
a
substantial
gap
our
understanding
of
how
influences
toxicity.
Herein,
by
exposing
Abstract
Background
Aquaporin-5
(AQP5)
is
a
crucial
membrane
protein
involved
in
water
transport
across
cellular
membranes,
particularly
within
exocrine
glands
such
as
salivary
glands.
Dysregulation
of
AQP5,
including
its
mislocalization,
has
been
associated
with
various
diseases,
emphasizing
the
need
to
understand
molecular
mechanisms
governing
trafficking.
This
study
investigates
multifaceted
regulatory
AQP5
trafficking,
specific
emphasis
on
role
carboxyl-terminal
(C-terminal)
tail
and
functional
involvement
prolactin-inducible
(PIP)
an
interacting
partner.
Methods
An
innovative
2D-custom
model
employing
SNAP-tag
human
constructs
together
novel
automated
algorithm-based
methodology
was
used
following
immunofluorescence
confocal
microscopy
assess
hAQP5
localization
plasma
stably
transfected
normal
gland-SV40
transformed-acinar
cells
(NS-SV-AC).
The
expression
verified
by
Western
blot
analysis.
Results
SNAP-hAQP5
expressed
NS-SV-AC
allowed
explore
C-terminal
hAQP5-hPIP
interaction
trafficking
upon
stimulation.
use
truncation
revealed
distinct
responses
intracellular
3’,5’-cyclic
adenosine
monophosphate
(cAMP)
calcium
increase,
shedding
light
importance
regions
highly
flexible
distal
part
for
Furthermore,
our
investigation
interplay
between
hPIP
that
PIP
promotes
translocation
membrane,
blunting
effects
calcium-
cAMP-dependent
pathways
sub-cellular
localization.
Conclusion
In
summary,
this
advances
understanding
dynamics
provides
critical
insights
into
roles
PIP.
sets
stage
future
investigations
identify
individual
amino
acids
phosphorylation
sites
C-terminus
mechanism
protein-protein
interaction,
dynamic
process
high
resolution
live
cell
imaging.
Further
research
area
warranted
uncover
regulation
offering
opportunities
development
therapeutic
strategies.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(8), С. 7403 - 7403
Опубликована: Апрель 17, 2023
Human
mitochondria
contain
a
circular
genome
that
encodes
13
subunits
of
the
oxidative
phosphorylation
system.
In
addition
to
their
role
as
powerhouses
cells,
are
also
involved
in
innate
immunity
mitochondrial
generates
long
double-stranded
RNAs
(dsRNAs)
can
activate
dsRNA-sensing
pattern
recognition
receptors.
Recent
evidence
shows
these
dsRNAs
(mt-dsRNAs)
closely
associated
with
pathogenesis
human
diseases
accompany
inflammation
and
aberrant
immune
activation,
such
Huntington’s
disease,
osteoarthritis,
autoimmune
Sjögren’s
syndrome.
Yet,
small
chemicals
protect
cells
from
mt-dsRNA-mediated
response
remain
largely
unexplored.
Here,
we
investigate
potential
resveratrol
(RES),
plant-derived
polyphenol
antioxidant
properties,
on
suppressing
activation.
We
show
RES
revert
downstream
immunogenic
stressors
elevate
RNA
expressions,
stimulation
by
exogenous
or
inhibition
ATP
synthase.
Through
high-throughput
sequencing,
find
regulate
mt-dsRNA
expression,
interferon
response,
other
cellular
responses
induced
stressors.
Notably,
treatment
fails
counter
effect
an
endoplasmic
reticulum
stressor
does
not
affect
expression
RNAs.
Overall,
our
study
demonstrates
usage
alleviate
stress
response.
Investigative Ophthalmology & Visual Science,
Год журнала:
2024,
Номер
65(5), С. 30 - 30
Опубликована: Май 21, 2024
Purpose:
Earlier
reports
highlighted
the
predominant
presence
of
aquaporin
4
(AQP4)
in
duct
cells
rabbit
lacrimal
glands
(LGs).
Whereas
significant
alterations
AQP4
mRNA
levels
have
been
observed
experimental
dry
eye
and
during
pregnancy,
impact
LG
ductal
fluid
production
remains
unclear.
In
our
recent
work,
role
secretion
was
investigated
utilizing
wild
type
(WT)
knock
out
(KO)
mice.
Methods:
Tear
assessed
both
WT
KO
animals.
Immunostaining
used
to
identify
protein.
Duct
segments
were
harvested
from
LGs
Fluid
filtration
permeability
(Pf)
quantified
using
video-microscopy.
Ductal
tear
production,
elicited
by
a
cell-permeable
cAMP
analogue
(8-bromo
cAMP),
carbachol,
vasoactive
intestinal
peptide
(VIP),
phenylephrine
(PHE),
ducts.
Results:
A
higher
expression
protein
noted
mice
when
compared
acinar
cells.
Pf
did
not
show
notable
between
Carbachol
comparable
secretory
responses
ducts
However,
8-bromo
cAMP,
VIP,
PHE
stimulation
resulted
decreased
LGs.
Conclusions:
Our
findings
underscore
functional
relevance
mouse
seems
play
different
roles
secretions
secretagogues.
Specifically,
cAMP-mediated,
adrenergic
agonist-related
reduced
