Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(12), С. 6481 - 6490
Опубликована: Март 14, 2024
Chrysin,
a
flavonoid,
has
been
found
to
have
widely
used
in
the
health
food
field.
But
at
present,
chrysin
production
is
hindered
by
low
availability
of
precursors
and
lack
catalytic
enzymes
with
high
activity.
Therefore,
ZmPAL
was
initially
screened
synthesize
trans-cinnamic
acid
activity
specificity.
To
enhance
supply
precursors,
shikimic
chorismic
pathway
genes
were
overexpressed.
Besides,
expression
intracellular
mitochondrial
carbon
metabolism
CIT,
MAC1/3,
CTP1,
YHM2,
RtME,
MDH
enhanced
increase
acetyl-CoA
content.
Chrysin
synthesized
through
novel
gene
combination
ScCPR–EbFNSI-1
PcFNSI.
Finally,
de
novo
synthesis
achieved,
reaching
41.9
mg/L,
which
highest
reported
concentration
date.
In
summary,
we
identified
efficient
for
increased
it
regulating
mitochondria
cytoplasm,
laying
foundation
future
large-scale
production.
ACS Synthetic Biology,
Год журнала:
2024,
Номер
13(4), С. 1059 - 1076
Опубликована: Март 28, 2024
Triterpenoids
possess
a
range
of
biological
activities
and
are
extensively
utilized
in
the
pharmaceutical,
food,
cosmetic,
chemical
industries.
Traditionally,
they
acquired
through
synthesis
plant
extraction.
However,
these
methods
have
drawbacks,
including
high
energy
consumption,
environmental
pollution,
being
time-consuming.
Recently,
de
novo
triterpenoids
microbial
cell
factories
has
been
achieved.
This
represents
promising
environmentally
friendly
alternative
to
traditional
supply
methods.
Saccharomyces
cerevisiae,
known
for
its
robustness,
safety,
ample
precursor
supply,
stands
out
as
an
ideal
candidate
triterpenoid
biosynthesis.
challenges
persist
industrial
production
economic
feasibility
Consequently,
metabolic
engineering
approaches
applied
improve
yield,
leading
substantial
progress.
review
explores
biosynthesis
mechanisms
S.
cerevisiae
strategies
efficient
production.
Finally,
also
discusses
current
proposes
potential
solutions,
offering
insights
future
engineering.
Microorganisms,
Год журнала:
2025,
Номер
13(2), С. 390 - 390
Опубликована: Фев. 10, 2025
Natamycin
is
a
polyene
macrocyclic
antibiotic
extensively
used
in
food,
medical,
and
agricultural
industries.
However,
its
high
production
cost
low
synthetic
efficiency
fail
to
meet
the
growing
market
demand.
Therefore,
enhancing
of
natamycin-producing
strains
crucial
for
achieving
industrial-scale
production.
This
study
systematically
evaluated
16
mutagenesis
methods
identified
atmospheric
room
temperature
plasma
combined
with
2-deoxyglucose
tolerance
screening
as
optimal
strategy
natamycin
A
high-yield
mutant
strain,
AG-2,
was
obtained,
an
80%
increase
(1.53
g/L)
compared
original
strain.
Metabolic
analysis
revealed
that
glycolysis
pentose
phosphate
pathway
were
enhanced
while
tricarboxylic
acid
cycle
weakened,
significantly
increasing
supply
precursors
such
acetyl-CoA,
methylmalonyl-CoA,
reducing
power
NADPH.
Additionally,
overexpression
nitrogen
metabolism
regulatory
gene
glnR
promoted
glutamate
glutamine,
further
AG-2
1.85
g/L.
In
5
L
fermenter,
engineered
strain
AG-glnR
achieved
final
11.50
g/L,
1.67
times
higher
than
first
combine
regulation,
effectively
providing
novel
approach
efficient
synthesis
other
antibiotics.
