ACS Applied Nano Materials,
Год журнала:
2023,
Номер
6(20), С. 19392 - 19402
Опубликована: Окт. 11, 2023
The
reusability
of
immobilized
lipases,
including
their
hydrolysis
activity,
is
greatly
affected
by
lipase
leakage,
mass
transfer
channel
blockage,
and
compound
deposition
on
the
outside
surface.
In
this
study,
Candida
antarctica
B
(CALB)
was
Fe3O4
nanoparticle-polyacrylonitrile
(PAN)
composite
magnetic
beads
through
phase
inversion
(L/FP).
activity
investigated
using
p-nitrophenyl
palmitate
(p-npp)
as
a
model
ester.
To
overcome
limitations
in
ester
hydrolysis,
three
modification
strategies
were
employed.
First,
covalently
nanoparticles
modified
with
methallyl
(M9)
chloro
(M13)
silane
modifiers,
which
exhibited
faster
immobilization
reduced
leaching
higher
expression
stability.
Second,
poly(ethylene
glycol)
(PEG6000)
polylactic
acid
(PLA)
added
to
precursors
physically
modify
structures
L/FP,
resulting
larger
specific
surface
area
(15.26
m2·g–1)
smaller
average
pore
size
(19
nm).
beads,
L/FP-9-M
L/FP-13-M,
demonstrated
superior
long-term
over
250
cycles.
retained
63
34%
initial
at
100th
cycle
250th
cycle,
respectively.
L/FP-13-M
55
36%
Furthermore,
even
after
repeated
uses,
unit
activities
(U·gsupport–1)
both
remained
than
Novo
435.
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(42), С. 23345 - 23354
Опубликована: Окт. 8, 2024
Precisely
controlling
enzyme
conformation
to
enhance
catalytic
performance
is
a
highly
sought-after
yet
challenging
goal
in
the
immobilization
of
biocatalysts.
Excessively
strong
enzyme-carrier
interactions
can
restrict
dynamics
and
reduce
efficiency,
while
excessively
weak
may
lead
leakage,
thereby
reducing
reusability.
In
this
study,
we
developed
novel
strategy
finely
regulate
interaction
between
carrier
through
adjustment
ratio
amino
octadecyl
functional
groups.
The
expressed
activity
immobilized
lipase,
CRL@AOMR,
was
1.32-
2.34-fold
higher
than
that
monofunctional
macroporous
resin.
Moreover,
synthesis
various
phytosterol
esters
solvent-free
systems
conducted
as
model
reaction
investigate
utilization
CRL@AOMR
different
reactions.
Under
optimized
conditions,
an
impressive
yield
96.1%
for
oleate
achieved
76.2%
maintained
even
after
six
cycles
(288
h).
This
study
demonstrates
potential
feasibility
developing
strategies
via
dual
modification
alkyl
groups,
which
general
other
enzymes
with
surface
lysine.
Food Chemistry X,
Год журнала:
2023,
Номер
21, С. 101082 - 101082
Опубликована: Дек. 16, 2023
A
core–shell
hydrogel
bead
system
was
designed
to
maintain
the
catalytic
activity
of
phytase
and
protect
its
enzymatic
functionality
from
heat
treatment.
The
structure
consists
a
chitosan-phytase
complex
core
an
alginate-carrageenan
shell.
optimized
improve
encapsulation
efficiency
increase
thermal
stability
encapsulated
phytase.
After
treatment,
retained
∼
70
%
same
secondary
free
Fourier
transform
infrared
spectroscopy
indicated
strong
intermolecular
interactions
between
chitosan
in
core,
but
little
interaction
alginate
κ-carrageenan
shell,
this
supports
structural
functional
Differential
scanning
calorimetry
confirmed
that
had
higher
melting
point.
Encapsulating
can
enhance
phytase,
which
broadens
potential
applications
for
delivery.
Veterinary Sciences,
Год журнала:
2024,
Номер
11(2), С. 91 - 91
Опубликована: Фев. 15, 2024
In
this
study,
we
used
the
Manchurian
golden
breed
of
quails.
We
assessed
efficacy
food
additives
phytase
from
Obesumbacterium
proteus
encapsulated
in
recombinant
Yarrowia
lipolytica
yeast,
which
was
supplied
at
a
concentration
500
activity
units
per
kg
feed.
One
hundred
fifty
one-day-old
quails
were
distributed
into
six
treatment
groups.
The
results
showed
that
adding
O.
to
quails’
diets
improved
live
weight,
body
weight
gain,
and
feed
conversion
compared
those
control
groups
using
commercial
Aspergillus
ficuum.
obtained
during
experiments
indicate
high
degree
assimilation
phytate-containing
feeds
if
fed
by
other
can
conclude
class
D
is
an
expedient
additive
possessing
better
kinetic
features
PhyA
PhyC
classes
phytases
when
it
acts
inside
quail’s
chyme.
Phytic
acid
and
its
salts
are
the
major
storage
form
of
both
phosphate
myo-inositol,
especially
in
cereals.
chelates
multivalent
cations,
which
decreases
solubilized
these
nutrients
their
dietary
bioavailability
for
absorption
assimilation.
Increasing
micronutrient
from
economic
phytate-rich
food
can
be
achieved
by
subjecting
this
to
fermentation
phytase-producing
bacteria.
A
total
8
lactic
bacteria
were
isolated
different
fermented
samples.
Among
tested
ability
produce
phytase,
only
five
isolates
selected
as
promising
producers.
The
results
revealed
that
isolate
No.
4
produced
highest
phytase
levels
411
U/mL.
differentially
able
tolerate
acidic,
alkaline,
heat,
surfactant,
osmotic,
bile,
pancreatic
enzyme
stresses,
with
superiority
4,
demonstrated
most
desirable
probiotic
potentials
confirmed
principal
component
analysis
(PCA),
heat
map,
network
analysis.
Isolate
was
identified
16S
rRNA
sequencing
later
Pediococcus
pentosaceus
strain
NMP4762Ch
under
accession
number
MZ413646.
Moreover,
active
cells
P.
immobilized
alginate;
reusability
resulted
continuous
production
an
optimum
activity
432.0
U/mL
after
14
days,
decreased
time
reach
142.5
56
days.
maximum
shelf
stability
also
observed,
557.5
U/mL,
days
declined
time,
reaching
133.5
end
56th
day.
Since
humans
don't
naturally
utilizing
probiotics
is
important
combat
mineral
deficiencies.
(1)
The
study
using
the
quails
of
Manchurian
golden
breed
was
aimed
at
efficacy
food
supplement
based
on
micro-incapsulated
phytase
Obessumbacterium
proteus
in
recombinant
yeast
producer
Yarrowia
lipolytica
a
dose
500
FYT/kg
feed.
(2)
effect
poultry
diets
productivity
young
and
adult
assayed;
(3)
It
found
that
keeping
microencapsulated
O.
provided
highest
live
weight,
intensity
egg
production,
slaughter
yield
animals,
lowest
feed
conversion
compared
to
those
control
groups
getting
commercial
Aspergillus
ficuum.
Taken
together
data
obtained
our
experiments
indicate
high
degree
assimilation
phytate-containing
products
by
when
encapsulated
both
preparation
PhyA
class.
can
be
concluded
it
is
highly
expedient
promising
class
D
for
feeding,
which
shows
best
kinetic
features
phytases
PhyC
classes
working
chyme
poultry.
The
fishery
and
aquaculture
industries
are
dealing
with
more
issues
pertaining
to
sustainability,
disease
control,
environmental
impact
as
the
demand
for
seafood
around
world
rises.
unique
properties
versatility
of
nanochitosan
have
made
it
a
promising
solution
these
problems.
potential
uses
difficulties
in
fisheries
systems
examined
this
chapter.
prospects
multifaceted.
Through
targeted
drug
delivery,
improved
management,
optimized
feed
efficiency,
they
present
opportunities
sustainable
practices.
Nanochitosan
also
holds
promise
remediation,
customized
solutions,
eco-friendly
pesticide
applications.
However,
several
challenges
must
be
addressed
fully
realize
aquaculture.
Regulatory
approval
safety
assessments
essential
ensure
responsible
use
nanoparticles.
Cost-effectiveness
scalability
production
methods
achieved
meet
industry
demands.
Concerns
about
nanoparticle
accumulation
aquatic
organisms
long-term
ecological
impacts
necessitate
comprehensive
research.
