Bulletin of Shakarim University Technical Sciences,
Год журнала:
2024,
Номер
1(4(16)), С. 243 - 254
Опубликована: Дек. 27, 2024
Lignocellulose
biomass
(LCB)
is
an
important
resource
for
the
production
of
biofuels
and
other
highvalue
products.
The
main
components
LCB
are
cellulose
lignin,
which
difficult
to
decompose.
Recent
research
has
focused
on
use
cellulolytic
microorganisms,
such
as
Bacillus
subtilis
bacteria,
Trichoderma
reesei
fungi
Penicillium
oxalicum,
effectively
break
down
these
components.
article
provides
overview
modern
achievements,
including
deep
eutectic
solvents
(DES),
showed
improvement
in
availability
increase
sugar
yield
up
80%.
agro-industrial
waste,
rice
straw,
a
substrate
enzymes,
helps
reduce
cost
supports
circular
bioeconomics.Strategies
efficiency
enzymatic
processing
described,
development
multi-enzyme
complexes
genetically
modified
strains
microorganisms.
For
example,
multifunctional
enzymes
from
Clostridium
cellulosi
allowed
sugars
by
30%.
possibilities
integrating
mushroom
co-cultures
hydrothermal
methods
that
provide
synergistic
effect
considered.Special
attention
paid
key
cellulases,
hemicellulases
ligninases
their
role
breakdown
plant
polymers.
Prospects
further
discussed,
thermostable
introduction
environmentally
friendly
integration
new
technologies
into
industrial
biorefineries.
Molecules,
Год журнала:
2024,
Номер
29(10), С. 2275 - 2275
Опубликована: Май 11, 2024
The
hydrolysis
and
biotransformation
of
lignocellulose,
i.e.,
biorefinery,
can
provide
human
beings
with
biofuels,
bio-based
chemicals,
materials,
is
an
important
technology
to
solve
the
fossil
energy
crisis
promote
global
sustainable
development.
Biorefinery
involves
steps
such
as
pretreatment,
saccharification,
fermentation,
researchers
have
developed
a
variety
biorefinery
strategies
optimize
process
reduce
costs
in
recent
years.
Lignocellulosic
hydrolysates
are
platforms
that
connect
saccharification
downstream
fermentation.
hydrolysate
composition
closely
related
biomass
raw
pretreatment
process,
choice
biorefining
strategies,
provides
not
only
nutrients
but
also
possible
inhibitors
for
In
this
review,
we
summarized
effects
each
stage
lignocellulosic
on
inhibitors,
analyzed
huge
differences
nutrient
retention
inhibitor
generation
among
various
emphasized
all
lignocellulose
need
be
considered
comprehensively
achieve
maximum
optimal
control
at
low
cost,
reference
development
chemicals.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Фев. 18, 2025
Abstract
Pistachio
dehulling
waste,
known
as
byproduct
mixture
(PBM),
is
a
valuable
resource
that
often
overlooked.
An
effective
sustainable
approach
involves
utilizing
this
agricultural
waste
through
fermentation
process
using
edible
filamentous
fungi,
demonstrating
potential
applications
in
nutrition
and
animal
feed.
The
focus
of
study
was
on
converting
PBM
extract
obtained
from
hot
water
extraction
pre-treatment
into
protein-rich
fungal
biomass
Neurospora
intermedia
.
optimal
conditions
for
growth
were
achieved
at
72
h,
pH
5.5,
30
°C
which
are
by
one-factor-at-a-time
(OFAT),
resulting
6.7
g/L
dried
biomass,
with
protein
content
20.4%.
conversion
efficiency,
expressed
grams
per
gram
initial
Total
COD,
0.37
g/g,
highlighting
the
significant
high
COD
levels
low
sugar
processes.
Additionally,
an
investigation
carried
out
to
assess
impact
inoculation
method,
culture
adaptation,
COD/N
ratio,
control
during
cultivation.
results
response
showed
production
0.44,
0.45,
0.49
g
total
contents
20.2%,
27.1%,
18.6%,
respectively,
leading
improved
yield.
biorefinery
platform
complete
value-added
cycle,
holds
promise
various
sectors
including
food,
feed,
biochemical,
biomaterial
industries.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Март 13, 2025
Biotransformation
of
a
pharmaceutical
precursor,
phenylacetylcarbinol
(PAC),
could
be
achieved
by
frozen-thawed
Candida
tropicalis
whole
cells
(FT-WHC).
The
treatment
FT-WHC,
which
contained
intracellular
pyruvate
decarboxylase
(PDC)
enzyme,
using
high-power
ultrasonication
with
varying
amplitudes
were
compared
glass
bead
attrition
(GBA)
as
well
control
for
the
release
PDC.
Ultrasonication
at
20%
amplitude
(Ult20)
proved
to
most
effective,
resulting
in
highest
volumetric
and
specific
PDC
activities
0.210
±
0.004
U/mL
0.335
0.033
U/mg
protein,
respectively.
Disrupted
FT-WHC
Ult20
exhibited
statistically
significant
(p
≤
0.05)
higher
initial
PAC
production
rate
(3.26
0.04
mM).
comparison
three
organic
phases,
namely,
vegetable
oil
(Vg-Oil),
Vg-Oil
+
dipropylene
glycol
(DPG),
octanol
two-phase
emulsion
system
biotransformation
revealed
overall
concentration
28.9
0.1
mM
DPG
system.
novel
addition
helped
facilitating
partitioning
into
aqueous
phase,
stabilizing
activity,
productivity
combination
treatment.
Abstract
Efficient
bioconversion
of
acetate-rich
lignocellulosic
biomass
into
value-added
chemicals
remains
a
major
challenge
due
to
the
toxicity
acetic
acid.
In
this
study,
we
engineered
an
acid-tolerant
Issatchenkia
orientalis
strain
(IoDY01H)
capable
producing
3-hydroxypropionic
acid
(3-HP),
key
bioplastic
precursor,
from
glucose,
xylose,
and
acetate.
Using
Cas9-based
genome
editing
system
with
hygromycin
B
resistance
marker,
introduced
heterologous
genes
encoding
xylose
utilization
β-alanine-based
3-HP
biosynthetic
pathways
I.
genome.
Metabolomic
analysis
revealed
that
acetate
supplementation
redirected
metabolic
flux
toward
amino
lipid
metabolism
while
reducing
TCA
cycle
intermediates.
Acetate
enhanced
production
by
promoting
accumulation
β-alanine,
but
also
β-alanine–pyruvate
aminotransferase
as
bottleneck
under
acidic
conditions.
pretreated
hemp
stalk
hydrolysate
feedstock,
achieved
titer
8.7
g/L
via
separate
hydrolysis
fermentation
(SHF),
outperforming
simultaneous
saccharification
(SSF).
These
findings
demonstrate
feasibility
using
non-conventional
yeast
highlight
promising
microbial
chassis
for
industrial
bioconversion.
Graphical
abstract
Highlights
Engineered
co-utilized
produce
3-HP.
addition
during
fermentation.
biosynthesis
pathways.
SHF.
Sustainability,
Год журнала:
2024,
Номер
16(5), С. 1788 - 1788
Опубликована: Фев. 22, 2024
Bioethanol,
as
a
renewable
energy
source,
has
been
widely
used
in
the
sector,
particularly
replacing
traditional
petroleum
energy,
and
holds
great
potential.
This
study
involves
whole
life
cycle
assessment
of
bioethanol
production
co-production
high-value
by-products—xylose,
lignin,
steam—using
three
types
waste
biomass:
corn
cobs,
straw,
wheat
straw
feedstocks
by
chopping,
pretreatment,
hydrolysis,
fermentation,
distillation
methods.
Secondly,
benefits
raw
materials
are
compared
for
preparing
bioethanol,
their
impact
on
environment
is
analyzed.
The
comparison
indicates
that
cobs
offer
best
overall
benefits,
with
net
balance
(NEB)
6902
MJ/Mg
ethanol
ratio
(NER)
1.30.
global
warming
potential
(GWP)
1.75
×
10−2,
acidification
(AP)
1.02
eutrophication
(EP)
2.63
10−4,
photochemical
ozone
creation
(POCP)
3.19
10−8,
human
toxicity
(HTP)
1.52
10−4.
paper
can
provide
theoretical
reference
data
supporting
green
refining
utilization
by-products,
broaden
its
application
prospects.
