SERS-based CRISPR/Cas assay on microfluidic paper analytical devices for supersensitive detection of pathogenic bacteria in foods

Biosensors and Bioelectronics, Год журнала: 2022, Номер 207, С. 114167 - 114167

Опубликована: Март 17, 2022

Язык: Английский

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Advances in nucleic acid amplification techniques (NAATs): COVID-19 point-of-care diagnostics as an example

Biosensors and Bioelectronics, Год журнала: 2022, Номер 206, С. 114109 - 114109

Опубликована: Фев. 26, 2022

Язык: Английский

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Novel non-nucleic acid targets detection strategies based on CRISPR/Cas toolboxes: A review

Biosensors and Bioelectronics, Год журнала: 2022, Номер 215, С. 114559 - 114559

Опубликована: Июль 13, 2022

Язык: Английский

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CRISPR‐Cas Biochemistry and CRISPR‐Based Molecular Diagnostics

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(17)

Опубликована: Янв. 30, 2023

Abstract Polymerase chain reaction (PCR)‐based nucleic acid testing has played a critical role in disease diagnostics, pathogen surveillance, and many more. However, this method requires long turnaround time, expensive equipment, trained personnel, limiting its widespread availability diagnostic capacity. On the other hand, clustered regularly interspaced short palindromic repeats (CRISPR) technology recently demonstrated capability for detection with high sensitivity specificity. CRISPR‐mediated biosensing holds great promise revolutionizing procedures developing point‐of‐care diagnostics. This review focuses on recent developments both fundamental CRISPR biochemistry CRISPR‐based techniques. Four ongoing research hotspots molecular diagnostics‐target preamplification‐free detection, microRNA (miRNA) testing, non‐nucleic‐acid SARS‐CoV‐2 detection‐are also covered.

Язык: Английский

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CRISPR‐Cas‐based detection for food safety problems: Current status, challenges, and opportunities

Comprehensive Reviews in Food Science and Food Safety, Год журнала: 2022, Номер 21(4), С. 3770 - 3798

Опубликована: Июль 1, 2022

Abstract Food safety is one of the biggest public issues occurring around world. Microbiological, chemical, and physical hazards can lead to food issues, which may occur at all stages supply chain. In order tackle safeguard consumer health, rapid, accurate, specific, field‐deployable detection methods meeting diverse requirements are imperative measures for assurance. CRISPR‐Cas system, a newly emerging technology, has been successfully repurposed in biosensing demonstrated huge potential establish conceptually novel with high sensitivity specificity. This review focuses on CRISPR‐Cas–based its current status specifically inspection. We firstly illustrate pending problems summarize popular methods. then describe applications Finally, challenges futuristic opportunities proposed discussed. Generally speaking, still unsatisfactory some ways such as being time‐consuming, displaying unmet specificity standards, there comparative paucity multiplexed testing POCT. Recent studies have shown that an innovative fast‐expanding could make up shortcomings existing or even replace them. To sum up, implementation integration other techniques promising desirable, expected provide “customized” “smart” inspection coming future.

Язык: Английский

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A SERS-signalled, CRISPR/Cas-powered bioassay for amplification-free and anti-interference detection of SARS-CoV-2 in foods and environmental samples using a single tube-in-tube vessel

Journal of Hazardous Materials, Год журнала: 2023, Номер 452, С. 131195 - 131195

Опубликована: Март 11, 2023

Язык: Английский

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Sensitive Small Molecule Aptasensing based on Hybridization Chain Reaction and CRISPR/Cas12a Using a Portable 3D-Printed Visualizer

ACS Sensors, Год журнала: 2023, Номер 8(3), С. 1076 - 1084

Опубликована: Янв. 18, 2023

Next-generation biosensing tools based on CRISPR/Cas have revolutionized the molecular detection. A number of CRISPR/Cas-based biosensors been reported for detection nucleic acid targets. The establishment efficient methods non-nucleic target would further broaden scope this technique, but up to now, concerning research is limited. In current study, we a versatile platform small-molecule called SMART-Cas12a (small-molecule aptamer regulated test using CRISPR/Cas12a). Simply, hybridization chain reaction cascade signal amplification was first trigged by functional (aptamer) through binding. Then, system integrated recognize amplified products followed activation trans-cleavage. As such, can be ingeniously converted signals and then fluorescent that readily visualized analyzed customized 3D-printed visualizer with help home-made App-enabled smartphone. Adenosine triphosphate selected as model target, under optimized conditions, achieved fine analytical performance linear range from 0.1 750 μM limit 1.0 nM. satisfactory selectivity recoveries obtained demonstrated method suitable complex sample environment. sample-to-answer time less than 100 min. Our work not only expanded reach CRISPR-Cas in also provided prototype generalized detecting wider analytes desirable adaptability, sensitivity, specificity, on-site capability.

Язык: Английский

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Sensitive and Amplification-Free Electrochemiluminescence Biosensor for HPV-16 Detection Based on CRISPR/Cas12a and DNA Tetrahedron Nanostructures

ACS Sensors, Год журнала: 2023, Номер 8(7), С. 2852 - 2858

Опубликована: Июль 4, 2023

Rapid and accurate detection of biomarkers was very important for early screening treatment diseases. Herein, a sensitive amplification-free electrochemiluminescence (ECL) biosensor based on CRISPR/Cas12a DNA tetrahedron nanostructures (TDNs) constructed. Briefly, 3D TDN self-assembled the Au nanoparticle-deposited glassy carbon electrode surface to construct biosensing interface. The presence target would activate trans-cleavage activity Cas12a-crRNA duplex cleave single-stranded signal probe vertex TDN, causing Ru(bpy)32+ fall from weakened ECL signal. Thus, system transduced change concentration into an enabling HPV-16. specific recognition HPV-16 made have good selectivity, while TDN-modified sensing interface could reduce cleaving steric resistance improve performance CRISPR/Cas12a. In addition, pretreated complete sample within 100 min with limit 8.86 fM, indicating that developed possesses potential application prospect fast nucleic acid detection.

Язык: Английский

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A ratiometric fluorescent biosensing platform for ultrasensitive detection of Salmonella typhimurium via CRISPR/Cas12a and silver nanoclusters

Journal of Hazardous Materials, Год журнала: 2022, Номер 443, С. 130234 - 130234

Опубликована: Окт. 27, 2022

Язык: Английский

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Development and Characterization of Recombinase-Based Isothermal Amplification Assays (RPA/RAA) for the Rapid Detection of Monkeypox Virus

Viruses, Год журнала: 2022, Номер 14(10), С. 2112 - 2112

Опубликована: Сен. 23, 2022

Monkeypox is a zoonotic disease caused by monkeypox virus (MPXV), in which outbreaks mainly occurred West and Central Africa, with only sporadic spillovers to countries outside Africa due international travel or close contact wildlife. During May 2022, multiple Europe, North South America, Australia, Asia, reported near-simultaneous of MPXV, the first time that patient clusters were over such large geographical area. Cases have no known epidemiological links MPXV-endemic Africa. Real-time PCR currently gold standard for MPXV diagnostics, but it requires trained laboratory personnel specialized equipment, results can be obtained after several hours. A rapid simple-to-operate point-of-care diagnostic test crucial limiting its spread controlling outbreaks. Here, three recombinase-based isothermal amplification assays (RPA/RAA) detection isolates developed. These target G2R gene, limit these systems approximately 100 copies DNA per reaction. The found specific non-cross reactive against other pox viruses, as vaccinia virus, visualized within 20-30 min. validated extracted from 19 clinical samples suspected confirmed patients consistent findings traditional qPCR. provide solid platform early diagnosis potential cases, will help control prevention current future

Язык: Английский

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