Abstract
Peroxidase
(POD)
Nanozyme‐based
hydrogen
peroxide
(H
2
O
)
detection
is
popular,
but
hardly
adapt
to
high
concentration
of
H
owing
narrow
linear
range
(LR)
and
low
LR
maximum.
Here,
a
solution
combining
POD
catalase
(CAT)
raised
expand
the
assay
via
decomposing
part
.
As
proof
concept,
cascade
enzyme
system
(rGRC)
constructed
by
integrating
ruthenium
nanoparticles
(RuNPs),
CAT
graphene
together.
The
rGRC‐based
sensor
does
perform
an
expanded
higher
maximum
for
detection.
Meanwhile,
it
confirmed
that
expansion
closely
associated
with
apparent
K
m
rGRC,
which
determined
relative
activity
between
both
in
theory
experiment.
At
last,
rGRC
successfully
used
detect
(up
10
contact
lens
care
solution,
performs
accuracy
(close
100%
recovery
at
than
traditional
nanozymes.
This
study
brings
up
kind
POD/CAT
provides
new
concept
accurate
facile
Additionally,
replenishes
enzyme‐substrate
model
achieving
same
pattern
competitive
inhibition
reactions.
Abstract
Nanozymes
show
great
promise
in
enhancing
disease
biomarker
sensing
by
leveraging
their
physicochemical
properties
and
enzymatic
activities.
These
qualities
facilitate
signal
amplification
matrix
effects
reduction,
thus
boosting
performance.
In
this
review,
recent
studies
from
the
last
five
years,
concentrating
on
detection
improvement
through
nanozyme‐based
biosensing
are
examined.
This
enhancement
primarily
involves
modulations
of
size,
morphology,
doping,
modification,
electromagnetic
mechanisms,
electron
conduction
efficiency,
surface
plasmon
resonance
nanozymes
for
increased
sensitivity.
addition,
a
comprehensive
description
synthesis
tuning
strategies
employed
has
been
provided.
includes
detailed
elucidation
catalytic
mechanisms
alignment
with
fundamental
principles
enhanced
technology,
accompanied
presentation
quantitatively
analyzed
results.
Moreover,
diverse
applications
strip
sensing,
colorimetric
electrochemical
surface‐enhanced
Raman
scattering
have
outlined.
Additionally,
limitations,
challenges,
corresponding
recommendations
concerning
application
summarized.
Furthermore,
insights
offered
into
future
development
outlook
biosensing.
review
aims
to
serve
not
only
as
reference
sensitivity
biosensors
but
also
catalyst
exploring
nanozyme
broader
ACS Applied Materials & Interfaces,
Год журнала:
2024,
Номер
16(9), С. 11228 - 11238
Опубликована: Фев. 25, 2024
Bacterial
infections
are
the
second
leading
cause
of
death
around
world,
especially
those
caused
by
delayed
treatment
and
misdiagnosis.
Therefore,
rapid
discrimination
effective
elimination
multiple
bacteria
great
importance
for
improving
survival
rate
in
clinic.
Herein,
a
novel
colorimetric
sensor
array
bacterial
is
constructed
using
programmable
DNA-encoded
iron
oxide
nanoparticles
(IONPs)
as
sensing
elements.
Utilizing
differential
interactions
on
IONPs,
11
kinds
dental
6
proteins
have
been
successfully
identified
linear
discriminant
analysis
(LDA).
Moreover,
developed
system
also
performs
well
quantitative
determination
individual
identification
mixtures.
More
importantly,
practicability
this
strategy
further
verified
precise
differentiation
blind
artificial
saliva
samples.
Furthermore,
used
efficiently
killing
bacteria,
demonstrating
potential
clinical
prophylaxis
therapy.
Analytical Chemistry,
Год журнала:
2024,
Номер
96(28), С. 11353 - 11365
Опубликована: Июль 6, 2024
Biothiols
play
essential
roles
in
maintaining
normal
physiological
functions,
resisting
oxidative
stress,
and
protecting
cell
health.
Establishing
an
effective
reliable
sensor
array
for
the
accurate
quantification
discrimination
of
diverse
biothiols
is
extremely
meaningful.
In
this
work,
Ag/Mn3O4,
Ag3PO4,
Ag3Cit
with
excellent
oxidase-mimetic
activity
surface-enhanced
Raman
scattering
(SERS)-enhanced
features
have
been
prepared
loaded
onto
Whatman
filter
paper
(WFP)
to
build
SERS
chips
as
three
sensing
channels,
which
can
induce
3,3′,5,5′-tetramethylbenzidine
(TMB)
oxidation
SERS-active
reporters
(TMBox)
concurrently
generate
prominent
signals.
Nevertheless,
addition
suppress
conversion
from
TMB
TMBox,
cause
reduction
signal
TMBox.
Interestingly,
each
channel
different
TMBox
signals'
variations
due
differences
inhibition
abilities
exclusive
properties
chip,
be
plotted
specific
fingerprint
patterns
biothiol
further
translated
into
intuitive
two-dimensional
(2D)
clustering
profiles
through
linear
discriminant
analysis
(LDA)
hierarchical
cluster
(HCA)
techniques
precise
identification
these
six
minimum
concentration
1
μM.
More
importantly,
exploited
intracellular
glutathione
(GSH),
differentiate
between
cancer
cells
based
on
GSH
contents
even
identify
types
tumor
cells,
demonstrating
its
powerful
application
prospects
disease
diagnosis.
Biomaterials Science,
Год журнала:
2024,
Номер
12(9), С. 2229 - 2243
Опубликована: Янв. 1, 2024
Nanozymes,
a
distinctive
class
of
nanomaterials
with
enzyme-like
activity
and
kinetics
akin
to
enzyme-catalysed
reactions,
present
several
advantages
over
natural
enzymes,
including
cost-effectiveness,
heightened
stability,
adjustable
activity.
