International Journal of Nanomedicine,
Год журнала:
2023,
Номер
Volume 18, С. 6311 - 6331
Опубликована: Ноя. 1, 2023
Abstract:
Recent,
outbreaks
of
new
emergency
zoonotic
diseases
have
prompted
an
urgent
need
to
develop
fast,
accurate,
and
portable
screening
assays
for
pathogen
infections.
Recombinase
polymerase
amplification
(RPA)
is
sensitive
specific
can
be
conducted
at
a
constant
low
temperature
with
short
response
time,
making
it
especially
suitable
on-site
powerful
tool
preventing
or
controlling
the
spread
zoonoses.
This
review
summarizes
design
principles
RPA-based
biosensors
as
well
various
signal
output
readout
technologies
involved
in
fluorescence
detection,
lateral
flow
assays,
enzymatic
catalytic
reactions,
spectroscopic
techniques,
electrochemical
chemiluminescence,
nanopore
sequencing
technologies,
microfluidic
digital
RPA,
clustered
regularly
interspaced
palindromic
repeats/CRISPR-associated
systems.
The
current
status
prospects
application
zoonoses
are
highlighted.
demonstrate
advantages
rapid
response,
easy-to-read
result
output,
easy
implementation
enabling
development
toward
greater
portability,
automation,
miniaturization.
Although
there
still
problems
such
high
cost
unstable
increasingly
becoming
one
most
important
means
complex
samples
involving
environmental,
water,
food,
animal,
human
diseases.
Keywords:
recombinase
amplification,
biosensor,
zoonoses,
nanomaterials
Frontiers in Microbiology,
Год журнала:
2024,
Номер
15
Опубликована: Июль 17, 2024
Loop-mediated
isothermal
amplification
(LAMP)
is
a
novel
method
for
nucleic
acid
detection
known
its
properties,
high
efficiency,
sensitivity,
and
specificity.
LAMP
employs
4
to
6
primers
targeting
8
regions
of
the
desired
sequence,
allowing
at
temperatures
between
60
65°C
production
up
10
Bioanalysis,
Год журнала:
2025,
Номер
unknown, С. 1 - 10
Опубликована: Фев. 4, 2025
CRISPR
(Clustered
Regularly
Interspaced
Short
Palindromic
Repeats)
has
transformed
molecular
biology
through
its
precise
gene-editing
capabilities.
Beyond
initial
applications
in
genetic
modification,
emerged
as
a
powerful
tool
diagnostics
and
biosensing.
This
review
explores
transition
from
genome
editing
to
innovative
detection
methods,
including
nucleic
acid
identification,
single
nucleotide
polymorphism
(SNP)
analysis,
protein
sensing.
Advanced
technologies
such
SHERLOCK
DETECTR
demonstrate
CRISPR's
potential
for
point-of-care
diagnostics,
enabling
rapid
highly
sensitive
detection.
The
integration
of
chemical
modifications,
CRISPR-Chip
technology,
enzymatic
systems
like
Cas12a
Cas13a
enhances
signal
amplification
efficiency.
These
advancements
promise
decentralized,
real-time
diagnostic
solutions
with
significant
implications
global
healthcare.
Furthermore,
the
fusion
artificial
intelligence
digital
health
platforms
is
paving
way
more
accessible,
cost-effective,
scalable
approaches,
ultimately
revolutionizing
precision
medicine.
TrAC Trends in Analytical Chemistry,
Год журнала:
2023,
Номер
162, С. 117028 - 117028
Опубликована: Март 18, 2023
In
addition
to
its
remarkable
genome
editing
capability,
the
CRISPR-Cas
system
has
proven
be
very
effective
in
many
fields
of
application,
including
biosensing
pathogenic
infections,
mutagenic
defects,
or
early
cancer
diagnosis.
Thanks
their
advantages
terms
simplicity,
efficiency,
and
reduced
time,
several
systems
have
been
described
for
design
sensitive
selective
analytical
tools,
paving
way
development
further
commercialization
next-generation
diagnostics.
However,
CRISPR-Cas-based
biosensors
still
need
research
efforts
improve
some
drawbacks,
such
as
target
amplification,
low
reproducibility,
lack
knowledge
exploited
element
robustness.
This
review
aims
describe
latest
trends
technologies
better
highlight
insights
point
out
limitations
that
overcome
future
market
entry
medical
Critical Reviews in Analytical Chemistry,
Год журнала:
2024,
Номер
unknown, С. 1 - 25
Опубликована: Сен. 17, 2024
This
comprehensive
review
delves
into
the
advancements
and
challenges
in
biosensing,
with
a
strong
emphasis
on
transformative
potential
of
CRISPR
technology
for
early
rapid
detection
infectious
diseases.
It
underscores
versatility
CRISPR/Cas
systems,
highlighting
their
ability
to
detect
both
nucleic
acids
non-nucleic
acid
targets,
seamless
integration
isothermal
amplification
techniques.
The
provides
thorough
examination
latest
developments
CRISPR-based
biosensors,
detailing
unique
properties
such
as
high
specificity
programmability,
which
make
them
particularly
effective
detecting
disease-associated
acids.
While
focuses
due
its
critical
role
diagnosing
diseases,
it
also
explores
broader
applications
thereby
acknowledging
technology's
potential.
Additionally,
identifies
existing
challenges,
need
improved
signal
real-world
applicability,
offers
future
perspectives
aimed
at
overcoming
these
hurdles.
ultimate
goal
is
advance
development
highly
sensitive
specific
biosensors
that
can
be
used
widely
improving
human
health,
point-of-care
settings
resource-limited
environments.
Designing
protein
mutants
with
both
high
stability
and
activity
is
a
critical
yet
challenging
task
in
engineering.
Here,
we
introduce
PRIME,
deep
learning
model,
which
can
suggest
improved
without
any
prior
experimental
mutagenesis
data
for
the
specified
protein.
Leveraging
temperature-aware
language
modeling,
PRIME
demonstrated
superior
predictive
ability
compared
to
current
state-of-the-art
models
on
public
dataset
across
283
assays.
Furthermore,
validated
PRIME’s
predictions
five
proteins,
examining
impact
of
top
30
45
single-site
mutations
various
properties,
including
thermal
stability,
antigen-antibody
binding
affinity,
polymerize
nonnatural
nucleic
acid
or
resilience
extreme
alkaline
conditions.
More
than
30%
PRIME-recommended
exhibited
performance
their
premutation
counterparts
all
proteins
desired
properties.
We
developed
an
efficient
effective
method
based
rapidly
obtain
multisite
enhanced
stability.
Hence,
demonstrates
broad
applicability
ACS Sensors,
Год журнала:
2023,
Номер
8(11), С. 3988 - 4007
Опубликована: Окт. 23, 2023
Point-of-care
(POC)
detection
is
getting
more
and
attention
in
many
fields
due
to
its
accuracy
on-site
test
property.
The
CRISPR/Cas12a
system
endowed
with
excellent
sensitivity,
target
identification
specificity,
signal
amplification
ability
biosensing
because
of
unique
trans-cleavage
ability.
As
a
result,
lot
research
has
been
made
develop
CRISPR/Cas12a-based
biosensors.
In
this
review,
we
focused
on
readout
strategies
summarized
recent
sensitivity-improving
fluorescence,
colorimetric,
electrochemical
signaling.
Then
introduced
novel
portability-improving
based
lateral
flow
assays
(LFAs),
microfluidic
chips,
simplified
instruments,
one-pot
design.
the
end,
also
provide
our
outlook
for
future
development
