Nanozyme-assisted amplification-free CRISPR/Cas system realizes visual detection DOI Creative Commons
Yuan Zhang, Wanpeng Yu, Man Wang

et al.

Frontiers in Bioengineering and Biotechnology, Journal Year: 2024, Volume and Issue: 11

Published: Jan. 5, 2024

The CRISPR (clustered regularly interspaced short palindromic repeats)/Cas (CRISPR associated) system has proven to be a powerful tool for nucleic acid detection due its inherent advantages of effective identification and editing capabilities, is therefore known as the next-generation molecular diagnostic technology. However, technologies based on CRISPR/Cas systems require preamplification target analytes; that is, gene amplification steps through isothermal or PCR before increase analyte concentrations. This creates number testing limitations, such extended time need more sophisticated instruments. To overcome above various amplification-free assay strategies have been explored alternatives, which omit step concentrations analytes. Nanozymes play pivotal role in enhancing sensitivity CRISPR-based detection, enabling visual rapid assays. utilization nanozyme exceptional enzyme-like catalytic activity holds great promise signal both electrochemical optical domains, encompassing sensors colorimetric sensors. Rather than relying converting single into multiple analytes, these methods focus amplification, main mechanism involves ability form large reporter molecules improve performance sensor. exploitation nanozymes results heightened accuracy outcomes. In addition sensor application nanozymes, additional are needed achieve without processes. Herein, we review improving do not preamplification, providing simple, intuitive preamplification-free platform by in-system one-step programs, nanozyme-mediated strategies.

Language: Английский

Advancements and applications of loop-mediated isothermal amplification technology: a comprehensive overview DOI Creative Commons
Nan Yang, Han Zhang,

Xiu Li Han

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: July 17, 2024

Loop-mediated isothermal amplification (LAMP) is a novel method for nucleic acid detection known its properties, high efficiency, sensitivity, and specificity. LAMP employs 4 to 6 primers targeting 8 regions of the desired sequence, allowing at temperatures between 60 65°C production up 10

Language: Английский

Citations

20
CRISPR in clinical diagnostics: bridging the gap between research and practice DOI
Zainab Lafi,

Tha’er Ata,

S. Asha

et al.

Bioanalysis, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 10

Published: Feb. 4, 2025

CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) has transformed molecular biology through its precise gene-editing capabilities. Beyond initial applications in genetic modification, emerged as a powerful tool diagnostics and biosensing. This review explores transition from genome editing to innovative detection methods, including nucleic acid identification, single nucleotide polymorphism (SNP) analysis, protein sensing. Advanced technologies such SHERLOCK DETECTR demonstrate CRISPR's potential for point-of-care diagnostics, enabling rapid highly sensitive detection. The integration of chemical modifications, CRISPR-Chip technology, enzymatic systems like Cas12a Cas13a enhances signal amplification efficiency. These advancements promise decentralized, real-time diagnostic solutions with significant implications global healthcare. Furthermore, the fusion artificial intelligence digital health platforms is paving way more accessible, cost-effective, scalable approaches, ultimately revolutionizing precision medicine.

Language: Английский

Citations

2
Multiplex bacteria detection using one-pot CRISPR/Cas13a-based droplet microfluidics DOI
Yuting Shang, Gaowa Xing,

Jiaxu Lin

et al.

Biosensors and Bioelectronics, Journal Year: 2023, Volume and Issue: 243, P. 115771 - 115771

Published: Oct. 18, 2023

Language: Английский

Citations

37
CRISPR-Cas assisted diagnostics: A broad application biosensing approach DOI Creative Commons
Annalisa Masi, Amina Antonacci, Maria Moccia

et al.

TrAC Trends in Analytical Chemistry, Journal Year: 2023, Volume and Issue: 162, P. 117028 - 117028

Published: March 18, 2023

In addition to its remarkable genome editing capability, the CRISPR-Cas system has proven be very effective in many fields of application, including biosensing pathogenic infections, mutagenic defects, or early cancer diagnosis. Thanks their advantages terms simplicity, efficiency, and reduced time, several systems have been described for design sensitive selective analytical tools, paving way development further commercialization next-generation diagnostics. However, CRISPR-Cas-based biosensors still need research efforts improve some drawbacks, such as target amplification, low reproducibility, lack knowledge exploited element robustness. This review aims describe latest trends technologies better highlight insights point out limitations that overcome future market entry medical

Language: Английский

Citations

30
CRISPR-Driven Biosensors: A New Frontier in Rapid and Accurate Disease Detection DOI

ZahraSadat Razavi,

M. Soltani, Mohammad Souri

et al.

Critical Reviews in Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 25

Published: Sept. 17, 2024

This comprehensive review delves into the advancements and challenges in biosensing, with a strong emphasis on transformative potential of CRISPR technology for early rapid detection infectious diseases. It underscores versatility CRISPR/Cas systems, highlighting their ability to detect both nucleic acids non-nucleic acid targets, seamless integration isothermal amplification techniques. The provides thorough examination latest developments CRISPR-based biosensors, detailing unique properties such as high specificity programmability, which make them particularly effective detecting disease-associated acids. While focuses due its critical role diagnosing diseases, it also explores broader applications thereby acknowledging technology's potential. Additionally, identifies existing challenges, need improved signal real-world applicability, offers future perspectives aimed at overcoming these hurdles. ultimate goal is advance development highly sensitive specific biosensors that can be used widely improving human health, point-of-care settings resource-limited environments.

Language: Английский

Citations

14
A general temperature-guided language model to design proteins of enhanced stability and activity DOI Creative Commons
Fan Jiang, Mingchen Li, Jiajun Dong

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(48)

Published: Nov. 27, 2024

Designing protein mutants with both high stability and activity is a critical yet challenging task in engineering. Here, we introduce PRIME, deep learning model, which can suggest improved without any prior experimental mutagenesis data for the specified protein. Leveraging temperature-aware language modeling, PRIME demonstrated superior predictive ability compared to current state-of-the-art models on public dataset across 283 assays. Furthermore, validated PRIME’s predictions five proteins, examining impact of top 30 45 single-site mutations various properties, including thermal stability, antigen-antibody binding affinity, polymerize nonnatural nucleic acid or resilience extreme alkaline conditions. More than 30% PRIME-recommended exhibited performance their premutation counterparts all proteins desired properties. We developed an efficient effective method based rapidly obtain multisite enhanced stability. Hence, demonstrates broad applicability

Language: Английский

Citations

11
Electrochemical aptasensor based on CRISPR/Cas12a-mediated and DNAzyme-assisted cascade dual-enzyme transformation strategy for zearalenone detection DOI
Yan Han, Baoshan He, Renyong Zhao

et al.

Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: 493, P. 152431 - 152431

Published: May 24, 2024

Language: Английский

Citations

10
Comprehensive insights into pathogenicity and emerging CRISPR-integrated biosensors for the early detection of breast cancer biomarkers DOI

Ashok Saini,

Neeraj Dilbaghi, Neelam Yadav

et al.

Microchemical Journal, Journal Year: 2025, Volume and Issue: unknown, P. 112908 - 112908

Published: Jan. 1, 2025

Language: Английский

Citations

1
Important applications of DNA nanotechnology combined with CRISPR/Cas systems in biotechnology DOI Creative Commons
Yuqi Huang, Zhongping Chen,

Huacui Huang

et al.

RSC Advances, Journal Year: 2025, Volume and Issue: 15(8), P. 6208 - 6230

Published: Jan. 1, 2025

Schematic illustration of several synthetic forms 2D and 3D DNA nanotechnology combined with CRISPR/Cas systems in biotechnology.

Language: Английский

Citations

1
Sensitive and Portable Signal Readout Strategies Boost Point-of-Care CRISPR/Cas12a Biosensors DOI
Tong Li, Nan Cheng

ACS Sensors, Journal Year: 2023, Volume and Issue: 8(11), P. 3988 - 4007

Published: Oct. 23, 2023

Point-of-care (POC) detection is getting more and attention in many fields due to its accuracy on-site test property. The CRISPR/Cas12a system endowed with excellent sensitivity, target identification specificity, signal amplification ability biosensing because of unique trans-cleavage ability. As a result, lot research has been made develop CRISPR/Cas12a-based biosensors. In this review, we focused on readout strategies summarized recent sensitivity-improving fluorescence, colorimetric, electrochemical signaling. Then introduced novel portability-improving based lateral flow assays (LFAs), microfluidic chips, simplified instruments, one-pot design. the end, also provide our outlook for future development

Language: Английский

Citations

20