LL-37_Renalexin hybrid peptide exhibits antimicrobial activity at lower MICs than its counterpart single peptides DOI Creative Commons
Julius Kwesi Narh, Néstor Casillas-Vega, Xristo Zárate

и другие.

Applied Microbiology and Biotechnology, Год журнала: 2024, Номер 108(1)

Опубликована: Янв. 13, 2024

Abstract An alarming global public health and economic peril has been the emergence of antibiotic resistance resulting from clinically relevant bacteria pathogens, including Enterococcus faecium , Staphylococcus aureus Klebsiella pneumonia Acinetobacter baumannii Pseudomonas aeruginosa Enterobacter species constantly exhibiting intrinsic extrinsic mechanisms against last-resort antibiotics like gentamycin, ciprofloxacin, tetracycline, colistin, standard ampicillin prescription in clinical practices. The discovery applications antimicrobial peptides (AMPs) with antibacterial properties have considered proven as alternative agents to antibiotics. In this study, we designed, produced, purified a recombinant novel multifunctional hybrid peptide LL-37_Renalexin for first time via application newly designed flexible GS linker coupled use our previously characterized small metal-binding proteins SmbP CusF3H+ carrier that allow an enhanced bacterial expression, using BL21(DE3) SHuffle T7(DE3) Escherichia coli strains, purification immobilized metal affinity chromatography. tag-free exhibited above 85% reduction colony-forming units broad-spectrum effects Methicillin-resistant (MRSA), pneumoniae isolates at lower minimum inhibition concentration level (10–33 μM) compared its counterpart single-AMPs LL-37 Renalexin (50–100 μM). Key points • linker. was expressed CusF3H+. shows potency isolates.

Язык: Английский

Biomimetic natural biomaterials for tissue engineering and regenerative medicine: new biosynthesis methods, recent advances, and emerging applications DOI Creative Commons
Shuai Liu, Jiangming Yu,

Yanchang Gan

и другие.

Military Medical Research, Год журнала: 2023, Номер 10(1)

Опубликована: Март 28, 2023

Biomimetic materials have emerged as attractive and competitive alternatives for tissue engineering (TE) regenerative medicine. In contrast to conventional biomaterials or synthetic materials, biomimetic scaffolds based on natural biomaterial can offer cells a broad spectrum of biochemical biophysical cues that mimic the in vivo extracellular matrix (ECM). Additionally, such mechanical adaptability, microstructure interconnectivity, inherent bioactivity, making them ideal design living implants specific applications TE This paper provides an overview recent progress (BNBMs), including advances their preparation, functionality, potential future challenges. We highlight fabrication BNBMs outline general strategies functionalizing tailoring with various biological physicochemical characteristics native ECM. Moreover, we key functionalization versatile applications. Finally, conclude by offering our perspective open challenges developments this rapidly-evolving field.

Язык: Английский

Процитировано

181
Photothermal antibacterial materials to promote wound healing DOI

Luning He,

Donghua Di,

Xinhui Chu

и другие.

Journal of Controlled Release, Год журнала: 2023, Номер 363, С. 180 - 200

Опубликована: Сен. 27, 2023

Язык: Английский

Процитировано

127
Advances in modified hyaluronic acid-based hydrogels for skin wound healing DOI
Yanwen Ding, Ze-Yu Wang, Zi-Wei Ren

и другие.

Biomaterials Science, Год журнала: 2022, Номер 10(13), С. 3393 - 3409

Опубликована: Янв. 1, 2022

Overview of crosslinking strategies hyaluronic acid-based hydrogels and their applications in skin wound healing.

Язык: Английский

Процитировано

105
Bioorthogonal Guided Activation of cGAS‐STING by AIE Photosensitizer Nanoparticles for Targeted Tumor Therapy and Imaging DOI

Minhui Cui,

Dongsheng Tang, Bin Wang

и другие.

Advanced Materials, Год журнала: 2023, Номер 35(52)

Опубликована: Сен. 5, 2023

Abstract Photodynamic therapy (PDT) and photothermal (PTT) leverage reactive oxygen species (ROS) control local hyperthermia by photosensitizer to perturb intracellular redox equilibrium, inducing DNA damage in both mitochondria nucleus, activating the cGAS‐STING pathway, ultimately eliciting antitumor immune responses. However, current photosensitizers are encumbered limitations such as suboptimal tumor targeting, aggregation‐caused quenching (ACQ), restricted excitation emission wavelengths. Here, this work designs novel nanoparticles based on aggregation‐induced (AIE) (BODTPE) for targeted near‐infrared II fluorescence imaging (NIR‐II FLI) with enhanced PDT/PTT effects. BODTPE is employed a monomer, dibenzocyclooctyne (DBCO)‐PEG 2k ‐amine serving an end‐capping polymer, synthesize BODTPE‐containing polymer (DBD). Further, through self‐assembly, DBD mPEG‐DSPE combined form (NP‐DBD). Notably, DBCO surface of NP‐DBD can react azide groups cancer cells pretreated Ac 4 ManNAz copper‐free click reaction. This innovative formulation led accumulation within sites, phenomenon convincingly demonstrated murine models subjected N ‐azidoacetylmannosamine‐tetraacylated (Ac ManNAz) pretreatment. Significantly, exhibits multifaceted effect encompassing PDT/PTT/NIR‐II FLI upon 808 nm laser irradiation, thereby better culminating compelling inhibition augmented robust modulation.

Язык: Английский

Процитировано

64
Antimicrobial Hydrogels: Potential Materials for Medical Application DOI Open Access
Yanni Li,

Yujia Han,

Hongxia Li

и другие.

Small, Год журнала: 2023, Номер 20(5)

Опубликована: Сен. 26, 2023

Abstract Microbial infections based on drug‐resistant pathogenic organisms following surgery or trauma and uncontrolled bleeding are the main causes of increased mortality from worldwide. The prevalence pathogens has led to a significant increase in medical costs poses great threat normal life people. This is an important issue field biomedicine, emergence new antimicrobial materials hydrogels holds promise for solving this problem. Hydrogel material with good biocompatibility, water absorption, oxygen permeability, adhesion, degradation, self‐healing, corrosion resistance, controlled release drugs as well structural diversity. Bacteria‐disturbing have applications direction surgical treatment, wound dressing, device coating, tissue engineering. paper reviews classification hydrogels, current status research, potential one application analyzes research biomedical five aspects: metal‐loaded drug‐loaded carbon‐material‐loaded fixed activity biological provides outlook high activity, biodegradability, injectability, clinical applicability future development prospects field.

Язык: Английский

Процитировано

48
Antimicrobial Peptides towards Clinical Application—A Long History to Be Concluded DOI Open Access

Laura Cresti,

Giovanni Cappello, Alessandro Pini

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(9), С. 4870 - 4870

Опубликована: Апрель 29, 2024

Antimicrobial peptides (AMPs) are molecules with an amphipathic structure that enables them to interact bacterial membranes. This interaction can lead membrane crossing and disruption pore formation, culminating in cell death. They produced naturally various organisms, including humans, animals, plants microorganisms. In higher they part of the innate immune system, where counteract infection by bacteria, fungi, viruses parasites. AMPs also be designed de novo bioinformatic approaches or selected from combinatorial libraries, then chemical recombinant procedures. Since their discovery, have aroused interest as potential antibiotics, although few reached market due stability limits toxicity. Here, we describe development phase a number clinical trials antimicrobial peptides. We provide update on pharmaceutical industry overall view therapeutic market. Modifications peptide structures improve vivo bioavailability described.

Язык: Английский

Процитировано

34
Comparison of poison plate and agar well diffusion method determining the antifungal activity of protein fractions DOI

Caleb Erhonyota,

Great Iruoghene Edo, Favour Ogheneoruese Onoharigho

и другие.

Acta Ecologica Sinica, Год журнала: 2022, Номер 43(4), С. 684 - 689

Опубликована: Сен. 2, 2022

Язык: Английский

Процитировано

56
Promotion of wound healing by a thermosensitive and sprayable hydrogel with nanozyme activity and anti-inflammatory properties DOI Creative Commons
Wei Zhang, Xingliang Dai,

Xu Jin

и другие.

Smart Materials in Medicine, Год журнала: 2022, Номер 4, С. 134 - 145

Опубликована: Сен. 6, 2022

The rapid healing of wounds requires strategies that relieve oxidative stress resulting from overloaded free radicals and which promote angiogenesis, collagen deposition, re-epithelialization the wound. Nickel ions have been reported to be correlated with angiogenesis. However, several applications metal salts or oxides lead increased toxicity. nickel metal-organic framework (Ni MOF) nanorods described herein can slowly release ions, in reduced toxicity improved wound rates. More importantly, Ni3(2,3,6,7,10,11-hexaiminotriphenylene)2 (Ni3(HITP)2) well-defined structures, superior conductivity many catalytic sites showed superoxide dismutase (SOD)-like enzyme activity scavenged various radicals. In addition, Ni3(HITP)2 nanomaterials contributed promotion migration fibroblasts, angiogenesis macrophage polarization M1 M2. aqueous solution Pluronic F127, a temperature-sensitive, nontoxic phase-changing hydrogel material, was shown an effective choice for injectable sprayable medical dressings. MOF effectively encapsulated F127 achieve continuous long-term therapeutic effects. test results suggested exhibited excellent biosafety no observable side effects mice. Therefore, hold promising potential biomedical field, this work provides therapy.

Язык: Английский

Процитировано

42
Advanced biosafety materials for prevention and theranostics of biosafety issues DOI Creative Commons
Jianxun Ding, Haihua Xiao, Xuesi Chen

и другие.

Biosafety and Health, Год журнала: 2022, Номер 4(2), С. 59 - 60

Опубликована: Март 17, 2022

Язык: Английский

Процитировано

40
Improving the Sustainability of Processing By-Products: Extraction and Recent Biological Activities of Collagen Peptides DOI Creative Commons

Shumin Xu,

Yuping Zhao,

Wenshan Song

и другие.

Foods, Год журнала: 2023, Номер 12(10), С. 1965 - 1965

Опубликована: Май 12, 2023

Society and consumers are increasingly concerned about food safety the sustainability of production systems. A significant amount by-products discards generated during processing aquatic animals, which still needs to be fully utilized by industry. The management sustainable use these resources essential avoiding environmental pollution resource waste. These rich in biologically active proteins, can converted into peptides enzymatic hydrolysis or fermentation treatment. Therefore, exploring extraction collagen from using an technology has attracted a wide range attention numerous researchers. Collagen have been found possess multiple biological activities, including antioxidant, anticancer, antitumor, hypotensive, hypoglycemic, anti-inflammatory properties. properties enhance physiological functions organisms make useful as ingredients food, pharmaceuticals, cosmetics. This paper reviews general methods for extracting various fish skin, scales, bones, offal. It also summarizes functional activities well their applications.

Язык: Английский

Процитировано

33