An overview of the therapeutic potential of understudied lichen species for novel drug discovery DOI Creative Commons

Aswathy Venugopal,

Stephey Glaudline,

David Paul Raj

и другие.

Journal of Applied and Natural Science, Год журнала: 2024, Номер 16(4), С. 1516 - 1529

Опубликована: Дек. 20, 2024

Due to the emergence of new diseases, drug discovery is an ever-exciting field science. Researchers have shifted their focus from chemical-based medicines drugs natural origin and structural synthetic analogues solve problem developing antibiotic resistance detrimental impacts existing chemical medications. Lichens are still one least explored sources for discovery. These slow-growing forms a rich source some exceptional secondary metabolites known collectively as lichenochemicals. The significant proportion these lichenochemicals bioactive compounds with multiple pharmacological properties, including antimicrobial, anticancer, free radical scavenging, anti-inflammatory, antidiabetic neuroprotective abilities also high in nutritional values, making this uncanny combination algae fungi, together metabolites, so well renowned traditional indigenous medicine. Although only oral knowledge was available about them earlier, studies over last few decades isolated identified unique medicinal lichen biology most interesting promising areas research. More than half species phytochemicals yet be explored. However, lichen-derived vitamin D3 vegan D3. Likewise, shown that can significantly reduce developed by microorganisms. Advanced techniques identification isolation, combined bioinformatics, predictive software, databases, greatly aided understanding chemistry. This review summarizes use lichens highlight on recent breakthroughs biology.

Язык: Английский

Screening Evaluation of Antiproliferative, Antimicrobial and Antioxidant Activity of Lichen Extracts and Secondary Metabolites In Vitro DOI Creative Commons
Martin Kello, Michal Goga, Klaudia Kotorová

и другие.

Plants, Год журнала: 2023, Номер 12(3), С. 611 - 611

Опубликована: Янв. 30, 2023

Lichen metabolites represent a wide range of substances with variety biological effects. The present study was designed to analyze the potential antiproliferative, antimicrobial and antioxidative effects several extracts from lichens (Pseudevernia furfuracea, Lobaria pulmonaria, Cetraria islandica, Evernia prunastri, Stereocaulon tomentosum, Xanthoria elegans Umbilicaria hirsuta) their secondary (atranorin, physodic acid, evernic acid gyrophoric acid). crude extract, as well isolated metabolites, showed potent cytotoxic activity on broad cancer cell lines in 2D (monolayer) 3D (spheroid) models. Furthermore, antioxidant (2,2-diphenyl-1-picryl-hydrazylhydrate (DPPH) vitro activities were assessed. Data that lichen extracts, compounds present, possessed studied assays. It also observed more efficient major strong antibacterial agents. Moreover, we demonstrated models' importance drug discovery for further vivo studies. Despite fact have been neglected by scientific community long periods, nowadays they are objects investigation based promising

Язык: Английский

Процитировано

24
SBFI26 induces triple‐negative breast cancer cells ferroptosis via lipid peroxidation DOI Creative Commons
Gang He, Yiyuan Zhang,

Yanjiao Feng

и другие.

Journal of Cellular and Molecular Medicine, Год журнала: 2024, Номер 28(7)

Опубликована: Март 22, 2024

Abstract SBFI26, an inhibitor of FABP5, has been shown to suppress the proliferation and metastasis tumour cells. However, underlying mechanism by which SBFI26 induces ferroptosis in breast cancer cells remains largely unknown. Three cell lines were treated with CCK‐8 assessed cytotoxicity. Transcriptome was performed on Illumina platform verified qPCR. Western blot evaluated protein levels. Malondialdehyde (MDA), total superoxide dismutase (T‐SOD), Fe, glutathione (GSH) oxidized (GSSG) measured. induced death time‐ dose‐dependent, a more significant inhibitory effect MDA‐MB‐231 Fer‐1, GSH Vitamin C attenuated effects but not erastin. RNA‐Seq analysis revealed that treatment significantly enriched differentially expressed genes related ferroptosis. Furthermore, increased intracellular MDA, iron ion, GSSG levels while decreasing T‐SOD, (T‐GSH), levels.SBFI26 dose‐dependently up‐regulates expression HMOX1 ALOX12 at both gene levels, promoting Similarly, it increases SAT1, ALOX5, ALOX15, ALOXE3 CHAC1 that, downregulating NFE2L2 inhibit leads cellular accumulation fatty acids, triggers excess ferrous ions subsequent lipid peroxidation for inducing

Язык: Английский

Процитировано

9
Lichens: An update on their ethnopharmacological uses and potential as sources of drug leads DOI
Olubukola T. Adenubi, Ibukun M. Famuyide, Lyndy J. McGaw

и другие.

Journal of Ethnopharmacology, Год журнала: 2022, Номер 298, С. 115657 - 115657

Опубликована: Авг. 22, 2022

Язык: Английский

Процитировано

29
Lichen Secondary Metabolites Inhibit the Wnt/β-Catenin Pathway in Glioblastoma Cells and Improve the Anticancer Effects of Temozolomide DOI Creative Commons
Aleksandra Majchrzak‐Celińska, Robert Kleszcz, Elżbieta Studzińska-Sroka

и другие.

Cells, Год журнала: 2022, Номер 11(7), С. 1084 - 1084

Опубликована: Март 23, 2022

Lichens are a source of secondary metabolites with significant pharmacological potential. Data regarding their possible application in glioblastoma (GBM) treatment are, however, scarce. The study aimed at analyzing the mechanism action six lichen metabolites: atranorin, caperatic acid, physodic squamatic salazinic and lecanoric acid using two- three-dimensional GBM cell line models. parallel artificial membrane permeation assay was used to predict blood-brain barrier penetration ability tested compounds. Their cytotoxicity analyzed MTT test on A-172, T98G, U-138 MG cells. Flow cytometry applied analysis oxidative stress, cycle distribution, apoptosis, whereas qPCR microarrays detected induced transcriptomic changes. Our data confirm cross exert against Moreover, compounds generated interfered cycle, apoptosis T98G They also inhibited Wnt/β-catenin pathway, this effect even stronger case co-treatment temozolomide. Transcriptomic changes cancer related genes by temozolomide were most pronounced. Lichen metabolites, particular, should be further as potential anti-GBM agents.

Язык: Английский

Процитировано

23
Diversity of tremellalean Pertusaria-associated fungi in Norway and the role of secondary metabolites in host specificity DOI Creative Commons
Mika H. Kirkhus, Andreas Frisch, Ann Evankow

и другие.

Fungal Biology, Год журнала: 2025, Номер 129(3), С. 101563 - 101563

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Unassuming Lichens: Nature’s Hidden Antimicrobial Warriors DOI Open Access

Hongqiao Tian,

Jie Lu, Fangrong Liang

и другие.

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3136 - 3136

Опубликована: Март 28, 2025

In a hidden corner of the Earth, an ongoing war is being waged: battle between lichens and microorganisms. Lichens, ancient unique symbiotic organisms, with their survival wisdom, are bursting vitality in extreme environments. Over 80% secondary metabolites not found other making lichen-derived compounds promising resource for development new drugs, particularly against drug-resistant microorganisms, due to distinctive chemical structures biological activities. This article aims explore depth lichen species exhibiting antimicrobial activity focus on such as divaricatic acid, usnic vulpinic salazinic rhizocarpic which demonstrate significant effects various resistant including methicillin-resistant Staphylococcus aureus, Mycobacterium tuberculosis, Candida albicans Meanwhile, this paper discusses potential applications challenges associated use medicine, agriculture, food industry, aiming elucidate these mysterious organisms researchers enthusiasts while promoting further research field antimicrobials.

Язык: Английский

Процитировано

0
Inhibition of thioredoxin reductase 1 by vulpinic acid suppresses the proliferation and migration of human breast carcinoma DOI
Şeyda Nur Kalın, Ahmet Altay, Harun Budak

и другие.

Life Sciences, Год журнала: 2022, Номер 310, С. 121093 - 121093

Опубликована: Окт. 18, 2022

Язык: Английский

Процитировано

17
Lichen Extracts from Cetrarioid Clade Provide Neuroprotection against Hydrogen Peroxide-Induced Oxidative Stress DOI Creative Commons
Isabel Ureña-Vacas, Elena González‐Burgos, Pradeep K. Divakar

и другие.

Molecules, Год журнала: 2022, Номер 27(19), С. 6520 - 6520

Опубликована: Окт. 2, 2022

Oxidative stress is involved in the pathophysiology of many neurodegenerative diseases. Lichens have antioxidant properties attributed to their own secondary metabolites with phenol groups. Very few studies delve into protective capacity lichens based on and action mechanism. The present study evaluates neuroprotective role Dactylina arctica, Nephromopsis stracheyi, Tuckermannopsis americana Vulpicida pinastri methanol extracts a hydrogen peroxide (H2O2) oxidative model neuroblastoma cell line "SH-SY5Y cells". Cells were pretreated different concentrations lichen (24 h) before H2O2 (250 µM, 1 h). Our results showed that D. arctica (10 µg/mL), N. stracheyi (25 T. (50 µg/mL) V. (5 prevented death morphological changes. Moreover, these significantly inhibited reactive oxygen species (ROS) production lipid peroxidation increased superoxide dismutase (SOD) catalase (CAT) activities glutathione (GSH) levels. Furthermore, they attenuated mitochondrial membrane potential decline calcium homeostasis disruption. Finally, high-performance liquid chromatography (HPLC) analysis revealed gyrophoric acid lecanoric artica, usnic acid, pinastric vulpinic pinastri, alectoronic americana. In conclusion, are most promising prevent treat stress-related

Язык: Английский

Процитировано

11
NGEF is a potential prognostic biomarker and could serve as an indicator for immunotherapy and chemotherapy in lung adenocarcinoma DOI Creative Commons
Xin Chen,

Tao Zhang,

Yan‐qiu He

и другие.

BMC Pulmonary Medicine, Год журнала: 2024, Номер 24(1)

Опубликована: Май 19, 2024

Abstract Background Neuronal guanine nucleotide exchange factor (NGEF) plays a key role in several cancers; however, its lung adenocarcinoma (LUAD) remains unclear. The aim of this study was to evaluate the efficacy NGEF as prognostic biomarker and potential therapeutic target for LUAD. Methods expression data multiple cancers LUAD were downloaded from databases. high- low-NGEF groups constructed based on median samples, then performed Kaplan–Meier survival analysis. Differentially expressed genes (DEGs) two screened applied construct protein-protein interaction network. primary pathways obtained using gene set enrichment associations between clinical characteristics, immune infiltration, checkpoint inhibitors (ICIs), sensitivity chemotherapy, tumor mutation burden (TMB) investigated R. Levels tissue validated single-cell RNA sequencing, quantitative polymerase chain reaction (qPCR), immunohistochemical staining, western blot Results mRNA upregulated cancers. protein levels higher patients with than controls, qPCR blot. High an independent associated advanced stage, large size, more lymph node metastasis, worse overall (OS). A total 182 overlapping DEGs Cancer Genome Atlas GSE31210, among which top 20 hub identified. mainly enriched apoptosis, cell cycle, DNA replication. Moreover, elevated high fraction activated memory CD4 + T cells M 0 macrophages; ICIs: programmed death 1 ligand expression; TMB; better bortezomib, docetaxel, paclitaxel, parthenolide, but less axitinib metformin. Conclusion is significantly stages, OS probability, immunotherapy response, chemotherapy response. may be diagnostic

Язык: Английский

Процитировано

2
Prospects of marine-derived compounds as potential therapeutic agents for glioma DOI Creative Commons
Ying Liu, Zhiyang Zhou, Shusen Sun

и другие.

Pharmaceutical Biology, Год журнала: 2024, Номер 62(1), С. 513 - 526

Опубликована: Июнь 12, 2024

Glioma, the most common primary malignant brain tumour, is a grave health concern associated with high morbidity and mortality. Current treatments, while effective to some extent, are often hindered by factors such as blood-brain barrier tumour microenvironment. This underscores pressing need for exploring new pharmacologically active anti-glioma compounds.

Язык: Английский

Процитировано

2