bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 2, 2025
ABSTRACT
Rhodamine
dyes
are
widely
used
fluorophores
in
super-resolution
fluorescence
imaging
due
to
their
exceptional
optical
properties
and
“aggregation-disaggregation”
induced
fluorogenic
activation.
However,
excessive
lipophilicity
often
reduces
brightness
aqueous
environments
causes
off-target
staining,
limiting
effectiveness
high-resolution
imaging.
To
address
these
challenges,
we
introduce
an
ether-decorated
N-terminal
modification
strategy
for
rhodamine
silicon-rhodamine
(Si-rhodamine),
replacing
conventional
N-alkyl
groups.
The
ether
chains
enhance
water
solubility,
decrease
aggregate
size,
improve
fluorogenicity
across
a
wide
concentration
range.
Their
flexible,
hydrophilic
structure
forms
protective
shield
around
the
xanthene
core,
minimizing
dye-water
interactions
reducing
quenching.
Additionally,
inductive
effect
of
decreases
electron-donating
strength
amino
groups,
suppressing
quenching
caused
by
twisted
intramolecular
charge
transfer
(TICT).
These
modifications
collectively
increase
quantum
yields
ER
ESiR
from
0.35
0.19
(for
tetraethyl-substituted
analogs)
0.70
0.41,
respectively.
Probes
derived
exhibit
outstanding
fluorogenicity,
enhanced
signal-to-noise
ratios,
improved
resolution
complex
environments,
demonstrating
superior
performance
advanced
techniques
such
as
structured
illumination
microscopy
(SIM),
stimulated
emission
depletion
(STED)
microscopy,
single-molecule
localization
(SMLM).
This
work
introduces
innovative
fluorophore
design,
offering
significant
advancements
applications.
Chemical Society Reviews,
Год журнала:
2021,
Номер
50(22), С. 12656 - 12678
Опубликована: Янв. 1, 2021
The
twisted
intramolecular
charge
transfer
(TICT)
mechanism
and
twists
beyond
TICT
have
guided
the
creation
of
numerous
bright
sensitive
fluorophores.
We
reviewed
structure–property
relationships
these
dyes
with
representative
examples.
Journal of the American Chemical Society,
Год журнала:
2023,
Номер
145(22), С. 12013 - 12022
Опубликована: Май 22, 2023
The
shortwave
infrared
(SWIR)
spectral
region
beyond
1200
nm
offers
optimal
tissue
penetration
depth
and
has
broad
potential
in
diagnosis,
therapy,
surgery.
Here,
we
devised
a
novel
class
of
fluorochromic
scaffold,
i.e.,
tetra-benzannulated
xanthenoid
(EC7).
EC7
absorbs/emits
maximally
at
1204/1290
CH2Cl2
exhibits
an
unparalleled
molar
absorptivity
3.91
×
105
cm–1
M–1
high
transparency
to
light
400–900
nm.
It
also
exhibited
resistance
toward
both
photobleaching
symmetry
breaking
due
its
unique
structural
rigidity.
is
feasible
for
vivo
bioimaging
particularly
suitable
couple
with
the
shorter-wavelength
analogues
high-contrast
multiplexing.
High-contrast
dual-channel
intraoperative
imaging
hepatobiliary
system
three-channel
intestine,
stomach,
vasculature
were
showcased.
benchmark
fluorochrome
facile
biomedical
exploitation
SWIR
Angewandte Chemie International Edition,
Год журнала:
2021,
Номер
60(47), С. 25104 - 25113
Опубликована: Сен. 14, 2021
Although
super-resolution
imaging
offers
an
opportunity
to
visualize
cellular
structures
and
organelles
at
the
nanoscale
level,
heterogeneity
unpredictability
still
pose
a
significant
challenge
in
dynamic
of
live
cells.
It
is
thus
vital
develop
better-performing
more
photostable
probes
for
long-term
imaging.
Herein,
we
report
probe,
LD-FG,
lipid
droplet
(LD)
dynamics
using
structured
illumination
microscopy
(SIM).
LD-FG
allows
wash-free
LDs,
owing
hydrogen-bond
sensitive
fluorogenic
response.
The
replacement
photobleached
by
intact
probe
molecules
outside
LDs
ensures
long-time
stability
fluorescence
With
this
buffering
fast
unpredictable
processes
can
be
visualized.
Using
two
LD
coalescence
modes
were
discovered.
also
allowed
us
propose
new
model
maturation
during
adipocyte
differentiation,
i.e.,
followed
slow
ripening
step.
excellent
performance
makes
buffer
strategy
effective
method
designing
fluorescent
cell
Journal of Materials Chemistry B,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 1, 2025
Near-infrared
fluorescence
imaging
is
key
in
biological
exploration.
Short
emission
wavelengths
of
xanthene
dyes
limit
their
use.
Structural
modifications
shift
emissions
to
the
NIR-I/II
range,
enhancing
biomedical
applications.
