Organic
fluorophores
with
near-infrared
(NIR)
emissions
and
reduced
molecular
weights
are
crucial
for
advancing
bioimaging
biosensing
technologies.
Traditional
methods,
such
as
conjugation
expansion
heteroatom
engineering,
often
fail
to
reduce
fluorophore
size
without
sacrificing
NIR
emission
properties.
Addressing
this
challenge,
our
study
utilized
computational
screening
structure-property
relationship
analysis
establish
comprehensive
design
principles
compact,
single-benzene-based
fluorophores.
These
newly
developed
not
only
exhibit
above
700
nm
but
also
maintain
under
200
g/mol,
approximately
25%
of
that
Cy7.
Additionally,
they
display
unique
environmental
sensitivity—non-emissive
in
aqueous
solutions
highly
emissive
lipid
environments.
This
property
significantly
enhances
their
utility
live
cell
imaging
by
enabling
wash-free
applications.
Our
findings
mark
a
substantial
breakthrough
paving
the
way
more
efficient
adaptable
methodologies.
Chemical Society Reviews,
Journal Year:
2021,
Volume and Issue:
50(22), P. 12656 - 12678
Published: Jan. 1, 2021
The
twisted
intramolecular
charge
transfer
(TICT)
mechanism
and
twists
beyond
TICT
have
guided
the
creation
of
numerous
bright
sensitive
fluorophores.
We
reviewed
structure–property
relationships
these
dyes
with
representative
examples.
Journal of the American Chemical Society,
Journal Year:
2023,
Volume and Issue:
145(22), P. 12013 - 12022
Published: May 22, 2023
The
shortwave
infrared
(SWIR)
spectral
region
beyond
1200
nm
offers
optimal
tissue
penetration
depth
and
has
broad
potential
in
diagnosis,
therapy,
surgery.
Here,
we
devised
a
novel
class
of
fluorochromic
scaffold,
i.e.,
tetra-benzannulated
xanthenoid
(EC7).
EC7
absorbs/emits
maximally
at
1204/1290
CH2Cl2
exhibits
an
unparalleled
molar
absorptivity
3.91
×
105
cm–1
M–1
high
transparency
to
light
400–900
nm.
It
also
exhibited
resistance
toward
both
photobleaching
symmetry
breaking
due
its
unique
structural
rigidity.
is
feasible
for
vivo
bioimaging
particularly
suitable
couple
with
the
shorter-wavelength
analogues
high-contrast
multiplexing.
High-contrast
dual-channel
intraoperative
imaging
hepatobiliary
system
three-channel
intestine,
stomach,
vasculature
were
showcased.
benchmark
fluorochrome
facile
biomedical
exploitation
SWIR
Angewandte Chemie International Edition,
Journal Year:
2021,
Volume and Issue:
60(47), P. 25104 - 25113
Published: Sept. 14, 2021
Although
super-resolution
imaging
offers
an
opportunity
to
visualize
cellular
structures
and
organelles
at
the
nanoscale
level,
heterogeneity
unpredictability
still
pose
a
significant
challenge
in
dynamic
of
live
cells.
It
is
thus
vital
develop
better-performing
more
photostable
probes
for
long-term
imaging.
Herein,
we
report
probe,
LD-FG,
lipid
droplet
(LD)
dynamics
using
structured
illumination
microscopy
(SIM).
LD-FG
allows
wash-free
LDs,
owing
hydrogen-bond
sensitive
fluorogenic
response.
The
replacement
photobleached
by
intact
probe
molecules
outside
LDs
ensures
long-time
stability
fluorescence
With
this
buffering
fast
unpredictable
processes
can
be
visualized.
Using
two
LD
coalescence
modes
were
discovered.
also
allowed
us
propose
new
model
maturation
during
adipocyte
differentiation,
i.e.,
followed
slow
ripening
step.
excellent
performance
makes
buffer
strategy
effective
method
designing
fluorescent
cell
Journal of Materials Chemistry B,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 1, 2025
Near-infrared
fluorescence
imaging
is
key
in
biological
exploration.
Short
emission
wavelengths
of
xanthene
dyes
limit
their
use.
Structural
modifications
shift
emissions
to
the
NIR-I/II
range,
enhancing
biomedical
applications.
