Near-infrared II fluorescence imaging

Nature Reviews Methods Primers, Год журнала: 2024, Номер 4(1)

Опубликована: Апрель 4, 2024

Язык: Английский

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Chemical Approaches to Optimize the Properties of Organic Fluorophores for Imaging and Sensing

Angewandte Chemie International Edition, Год журнала: 2023, Номер 63(11)

Опубликована: Дек. 12, 2023

Abstract Organic fluorophores are indispensable tools in cells, tissue and vivo imaging, have enabled much progress the wide range of biological biomedical fields. However, many available dyes suffer from insufficient performances, such as short absorption emission wavelength, low brightness, poor stability, small Stokes shift, unsuitable permeability, restricting their application advanced imaging technology complex imaging. Over past two decades, efforts been made to improve these performances fluorophores. Starting with luminescence principle fluorophores, this review clarifies mechanisms performance for traditional a certain extent, systematically summarizes modified approaches optimizing properties, highlights typical applications improved sensing, indicates existing problems challenges area. This not only proves significance improving but also provide theoretical guidance development high‐performance

Язык: Английский

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Strategies to convert organic fluorophores into red/near-infrared emitting analogues and their utilization in bioimaging probes

Chemical Society Reviews, Год журнала: 2023, Номер 52(18), С. 6344 - 6358

Опубликована: Янв. 1, 2023

This review analyzes strategies to convert key fluorophores into red/NIR emitting derivatives, with typical examples of how such can be used develop molecular probes for biological analytes, along sensing features.

Язык: Английский

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Biomimetic NIR-II fluorescent proteins created from chemogenic protein-seeking dyes for multicolor deep-tissue bioimaging

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Апрель 2, 2024

Abstract Near-infrared-I/II fluorescent proteins (NIR-I/II FPs) are crucial for in vivo imaging, yet the current NIR-I/II FPs face challenges including scarcity, requirement chromophore maturation, and limited emission wavelengths (typically < 800 nm). Here, we utilize synthetic protein-seeking NIR-II dyes as chromophores, which covalently bind to tag (e.g., human serum albumin, HSA) through a site-specific nucleophilic substitution reaction, thereby creating proof-of-concept biomimetic FPs. This chemogenic strategy can be accomplished under gentle physiological conditions without catalysis. Proteomics analysis identifies specific binding site (Cys 477 on DIII). significantly enhance brightness photostability, while improving biocompatibility, allowing high-performance lymphography angiography. is universal applicable wide range of spectrally separated real-time visualization multiple biological events. Overall, this straightforward approach holds potential transform protein-based bioimaging enables in-situ albumin targeting create deep-tissue imaging live organisms.

Язык: Английский

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An All‐Rounder for NIR‐II Phototheranostics: Well‐Tailored 1064 nm‐Excitable Molecule for Photothermal Combating of Orthotopic Breast Cancer

Angewandte Chemie International Edition, Год журнала: 2024, Номер 63(26)

Опубликована: Апрель 19, 2024

The second near-infrared (NIR-II, 1000-1700 nm) light-activated organic photothermal agent that synchronously enables satisfying NIR-II fluorescence imaging is highly warranted yet rather challenging on the basis of overwhelming nonradiative decay. Herein, such an agent, namely TPABT-TD, was tactfully designed and constructed via employing benzo[c]thiophene moiety as bulky electron donor/π-bridge tailoring peripheral molecular rotors. Benefitting from its high donor-acceptor strength finely modulated intramolecular motion, TPABT-TD simultaneously exhibits ultralong absorption in region, intense emission NIR-IIa (1300-1500 region nanoaggregates, conversion upon 1064 nm laser irradiation. Those intrinsic advantages endow nanoparticles with prominent fluorescence/photoacoustic/photothermal trimodal imaging-guided therapy against orthotopic 4T1 breast tumor negligible adverse effect.

Язык: Английский

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Rational Design of NIR‐II Ratiometric Fluorescence Probes for Accurate Bioimaging and Biosensing In Vivo

Small Methods, Год журнала: 2024, Номер unknown

Опубликована: Март 12, 2024

Abstract Intravital fluorescence imaging in the second near‐infrared window (NIR‐II, 900–1700 nm) has emerged as a promising method for non‐invasive diagnostics complex biological systems due to its advantages of less background interference, high tissue penetration depth, contrast, and sensitivity. However, traditional NIR‐II imaging, which is characterized by “always on” or “turn mode, lacks ability quantitative detection, leading low reproducibility reliability during bio‐detection. In ratiometric can realize reliable analysis detection vivo providing reference signals correction, generating new opportunities prospects bioimaging biosensing. this review, current design strategies sensing mechanisms probes biosensing applications are systematically summarized. Further, challenges, future perspectives designing also discussed. It hoped that review provide effective guidance promote adoption vivo.

Язык: Английский

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An Emerging Toolkit of Ho³⁺ Sensitized Lanthanide Nanocrystals with NIR-II Excitation and Emission for in Vivo Bioimaging

Journal of the American Chemical Society, Год журнала: 2025, Номер unknown

Опубликована: Янв. 2, 2025

Optical imaging in the second near-infrared window (NIR-II, 1000-1700 nm) holds great promise for biomedical detection due to reduced tissue scattering and autofluorescence. However, rational design of NIR-II probes with superior excitation wavelengths balance effects water absorption remains a challenge. To address this issue, here we developed series Ho

Язык: Английский

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A stable and biocompatible shortwave infrared nanoribbon for dual-channel in vivo imaging

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Янв. 2, 2025

The shortwave infrared (SWIR) region is an ideal spectral window for next-generation bioimaging to harness improved penetration and reduced phototoxicity. SWIR activity may also be accessed via supramolecular dye aggregation. Unfortunately, development of aggregation remains challenging. We propose a crystal-aided aggregate synthesis (CAASH) approach introduce layer rationality the J-aggregate successful water-soluble JV-aggregate with bisbenzannulated silicon rhodamine scaffold (ESi5). resulting SWIR-aggregates exhibit excellent stabilities toward organic solvents, pH, sonication, photobleaching, thiols, endogenous oxidative species. Notably, aggregates have high structure-dependent melting temperature ca. 330-335 K. In fact, heating/annealing process can exploited reduce disorder. are biocompatible broad potential in vivo fluorescence photoacoustic imaging more. While has applications, stable absorbers/emitters challenging access. Here, authors report J-aggregates bioimaging.

Язык: Английский

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Electron‐Withdrawing Substituents Allow Boosted NIR‐II Fluorescence in J‐Type Aggregates for Bioimaging and Information Encryption

Angewandte Chemie International Edition, Год журнала: 2023, Номер 62(47)

Опубликована: Окт. 11, 2023

Developing molecular fluorophores with enhanced fluorescence in aggregate state for the second near-infrared (NIR-II) imaging is highly desirable but remains a tremendous challenge due to lack of reliable design guidelines. Herein, we report an aromatic substituent strategy construct bright NIR-II J-aggregates. Introduction electron-withdrawing substituents at 3,5-aryl and meso positions classic boron dipyrromethene (BODIPY) skeleton can promote slip-stacked J-type arrangement further boost J-aggregates via increased electrostatic repulsion intermolecular hydrogen bond interaction. Notably, NOBDP-NO2 three nitro groups (-NO2 ) shows intense 1065 nm high absolute quantum yield 3.21 % solid state, which be successfully applied bioimaging, high-level encoding encryption, information storage. Moreover, guided by this strategy, other skeletons (thieno-fused BODIPY, aza-BODIPY, heptamethine cyanine) modified -NO2 are converted into aggregates fluorescence, showing great potential convert aggregation caused emission quenching (ACQ) dyes brilliant This study provides universal method construction strong emissive rationally manipulating packing establishing relationships among structures, interactions, properties.

Язык: Английский

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Two Key Descriptors for Designing Second Near-Infrared Dyes and Experimental Validation

Journal of the American Chemical Society, Год журнала: 2024, Номер 146(14), С. 9888 - 9896

Опубликована: Март 28, 2024

Second near-infrared (NIR-II) optical imaging technology has emerged as a powerful tool for diagnostic and image-guided surgery due to its higher contrast. However, general strategy efficiently designing NIR-II organic molecules is still lacking, because dyes are usually difficult synthesize, which impeded the rapid development of bioprobes. Herein, based on theoretical calculations 62 multiaryl-pyrrole (MAP) systems with spectra ranging from visible region, continuous red shift toward region could be achieved by adjusting type site substituents MAPs. Two descriptors (ΔEgs μgs) were identified exhibiting strong correlations maximum absorption/emission wavelengths, used predict emission spectrum in only if ΔEgs ≤ 2.5 eV μgs 22.55 D. The experimental absorption ten MAPs fully confirmed predictions, biological vivo newly designed MAP23-BBT showed high spatial resolution deep tissue angiography. More importantly, both have shown applicability most reported donor–acceptor–donor-type non-MAP dyes. These results broad implications efficient design

Язык: Английский

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