Aptamer-based assembly systems for SARS-CoV-2 detection and therapeutics

Chemical Society Reviews, Год журнала: 2024, Номер 53(13), С. 6830 - 6859

Опубликована: Янв. 1, 2024

This review presents the recent progress on aptamers that have been explored for SARS-CoV-2 detection and therapeutics, wherein construction principles characteristics of aptamer-based assembly systems are systematically summarized.

Язык: Английский

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Advancements in wound healing: integrating biomolecules, drug delivery carriers, and targeted therapeutics for enhanced tissue repair

Archives of Microbiology, Год журнала: 2024, Номер 206(4)

Опубликована: Апрель 1, 2024

Язык: Английский

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Net-Shaped DNA Nanostructure-Based Lateral Flow Assays for Rapid and Sensitive SARS-CoV-2 Detection

Analytical Chemistry, Год журнала: 2024, Номер 96(8), С. 3291 - 3299

Опубликована: Фев. 2, 2024

Lateral flow assay (LFA)-based rapid antigen tests are experiencing extensive global uptake as an expeditious and highly effective modality for the screening of viral infections during COVID-19 pandemic. While these devices have played a significant role in alleviating burden on public healthcare system, their specificity sensitivity fall short compared with molecular tests. In this study, we endeavor to address both limitations through utilization DNA nanotechnology LFA format, wherein substitute target-specific antibody designer nanostructure-based probes recognizing SARS-CoV-2 virus via multivalent, pattern-matching interactions. We meticulously designed Net-shaped nanostructure strategically arranged trimeric clusters aptamers that specifically recognize spike proteins SARS-CoV-2. This approach has proven instrumental bolstering virus-binding affinity LFAs. Our findings indicate high sensitivity, enabling detection loads ranging from 103 108 copies/mL. notable is maintained across various strains, obviating need intricate sample preparation protocols. The significance heightened lies crucial by nanostructure, which facilitates extremely low levels loads. not only enhances overall reliability self-testing but also reduces likelihood false-negative results, especially cases load within patient samples.

Язык: Английский

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Engineered two-dimensional nanomaterials based diagnostics integrated with internet of medical things (IoMT) for COVID-19

Chemical Society Reviews, Год журнала: 2024, Номер 53(8), С. 3774 - 3828

Опубликована: Янв. 1, 2024

Engineered 2D nanomaterials-based smartphone-assisted detection approaches integrated with IoMT, focusing on reliable, sensitive, and rapid diagnostics for COVID-19.

Язык: Английский

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A Poly Aptamer Encoded DNA Nanocatcher Informs Efficient Virus Trapping

Nano Letters, Год журнала: 2024, Номер 24(12), С. 3614 - 3623

Опубликована: Март 18, 2024

Broad-spectrum antiviral platforms are always desired but still lack the ability to cope with threats global public health. Herein, we develop a poly aptamer encoded DNA nanocatcher platform that can trap entire virus particles inhibit infection broad spectrum. Ultralong single-stranded (ssDNA) containing repeated aptamers was synthesized as scaffold of via biocatalytic process, wherein mineralization magnesium pyrophosphate on ssDNA could occur and consequently lead formation interfacial nanocaves decorated virus-binding aptamers. Once viruses were recognized by apatmers, they would be captured trapped in multisite synergistic interactions. Meanwhile, size nanocatchers optimized prevent their cellular uptake, which further guaranteed inhibition infection. By taking SARS-CoV-2 variants model target, demonstrated virus-trapping capability engulfing blocking host cells.

Язык: Английский

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Development of de-novo coronavirus 3-chymotrypsin-like protease (3CLpro) inhibitors since COVID-19 outbreak: A strategy to tackle challenges of persistent virus infection

European Journal of Medicinal Chemistry, Год журнала: 2023, Номер 264, С. 115979 - 115979

Опубликована: Ноя. 25, 2023

Язык: Английский

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Differential Diagnosis in the Management of Acute Respiratory Infections through Point-of-Care Rapid Testing in a Post-Pandemic Scenario in Latin America: Special Focus on COVID-19, Influenza, and Respiratory Syncytial Virus

COVID, Год журнала: 2024, Номер 4(2), С. 221 - 260

Опубликована: Фев. 10, 2024

This review provides a comprehensive summary of evidence to explore the role and value differential diagnosis in management Acute Respiratory Infections (ARIs) through point-of-care (POC) rapid testing post-pandemic scenario, paying particular attention coronavirus disease 2019 (COVID-19), influenza, respiratory syncytial virus (RSV). The document builds on literature policies process validation feedback by group seven experts from Latin America (LATAM). Evidence was collected understand scientific policy perspectives ARIs POC testing, with focus countries: Argentina, Brazil, Chile, Colombia, Costa Rica, Mexico, Peru. indicates that can serve improve ARI case management, epidemiological surveillance, research innovation, evidence-based decision-making. With multiple types tests available for POC, decisions regarding which use require consideration purpose, resources, test characteristics accuracy, accessibility, affordability, results turnaround time. Based understanding current situation, this set recommendations implementation LATAM, supporting decision-making guiding efforts broad range stakeholders.

Язык: Английский

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Advancements in SARS-CoV-2 detection: Navigating the molecular landscape and diagnostic technologies

Heliyon, Год журнала: 2024, Номер 10(9), С. e29909 - e29909

Опубликована: Апрель 21, 2024

According to information from the World Health Organization, world has experienced about 430 million cases of COVID-19, a world-wide health crisis caused by SARS-CoV-2 virus. This outbreak, originating China in 2019, led nearly 6 deaths worldwide. As number confirmed infections continues rise, need for cutting-edge techniques that can detect early and accurately become more critical. To address this, Federal Drug Administration (FDA) issued emergency use authorizations (EUAs) wide range diagnostic tools. These include tests based on detecting nucleic acids antigen-antibody reactions. The quantitative real-time reverse transcription PCR (qRT-PCR) assay stands out as gold standard virus detection. However, despite its accuracy, qRT-PCR limitations, such complex testing protocols risk false negatives, which drive continuous improvement acid serological approaches. emergence highly contagious variants coronavirus, Alpha (B.1.1.7), Delta (B.1.617.2), Omicron (B.1.1.529), increased specifically identify these mutations. article explores both acid-based assays, assessing performance recently approved FDA those documented scientific research, especially identifying new coronavirus strains.

Язык: Английский

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Recent advancements in the small-molecule drugs for hepatocellular carcinoma (HCC): Structure-activity relationships, pharmacological activities, and the clinical trials

Biomedicine & Pharmacotherapy, Год журнала: 2024, Номер 179, С. 117343 - 117343

Опубликована: Авг. 24, 2024

BACKGROUND AND AIMS: Hepatocellular carcinoma (HCC) is one of the most common malignancies in world and sixth leading cause cancer death worldwide, it urgent to find safe effective drugs for treatment. As an important therapeutic method, small-molecule are continually being updated achieve improved effects. The purpose this study was investigate structural effects various FDA-listed sorafenib, cabozantinib, lenvatinib, regorafenib on corresponding HCC targets possible optimization methods, explore mechanism identifying potential that offer better efficacy fewer side

Язык: Английский

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Application of a Primer Passivated CdTe Quantum Dots as a Novel Förster Resonance Energy Transfer based Nanobiosensor for Covid-19 Virus Detection

Colloids and Surfaces A Physicochemical and Engineering Aspects, Год журнала: 2025, Номер unknown, С. 136152 - 136152

Опубликована: Янв. 1, 2025

Язык: Английский

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