Molecules,
Год журнала:
2023,
Номер
28(18), С. 6565 - 6565
Опубликована: Сен. 11, 2023
The
drive
to
achieve
ultrasensitive
target
detection
with
exceptional
efficiency
and
accuracy
requires
the
advancement
of
immunoassays.
Optical
immunoassays
have
demonstrated
significant
potential
in
clinical
diagnosis,
food
safety,
environmental
protection,
other
fields.
Through
innovative
feasible
combination
enzyme
catalysis
optical
immunoassays,
notable
progress
has
been
made
enhancing
analytical
performances.
Among
kinds
reporter
enzymes,
alkaline
phosphatase
(ALP)
stands
out
due
its
high
catalytic
activity,
elevated
turnover
number,
broad
substrate
specificity,
rendering
it
an
excellent
candidate
for
development
various
This
review
provides
a
systematic
evaluation
advancements
by
employing
ALP
as
signal
label,
encompassing
fluorescence,
colorimetry,
chemiluminescence,
surface-enhanced
Raman
scattering.
Particular
emphasis
is
placed
on
fundamental
amplification
strategies
employed
ALP-linked
Furthermore,
this
work
briefly
discusses
proposed
solutions
challenges
that
need
be
addressed
further
enhance
performances
ACS Nano,
Год журнала:
2023,
Номер
17(19), С. 19359 - 19371
Опубликована: Окт. 2, 2023
The
multimodal
lateral
flow
immunoassay
(LFIA)
has
provided
accurate
and
reliable
results
for
fast
immediate
detection.
Nonetheless,
LFIA
remains
challenging
to
develop
biosensors
with
high
sensitivity
tolerance
matrix
interference
in
agro-food.
In
this
study,
we
developed
a
self-assembled
multivalent
fluorescence-nanobody
(Nb26-EGFP-H6)
16.5-fold
30-fold
higher
affinity
than
monovalent
nanobody
(Nb26).
Based
on
the
Nb26-EGFP-H6,
synthesized
enhanced
immune-probes
Zn-CN@Nb26-EGFP-H6
by
pyrolyzing
oxidizing
an
imidazolating
zeolite
framework-8
(ZIF-8)
obtain
photothermal
metal–carbon
nanomaterials
(Zn-CN)
immobilizing
Nb26-EGFP-H6.
rough
porous
structure
of
Zn-CN
large
surface
area
facilitates
enrichment
immobilization
antibodies.
A
trimodal
(tLFIA)
colorimetric,
fluorescent,
triple
signal
outputs
was
constructed
detection
aflatoxin
B1
(AFB1)
maize.
Attractively,
Zn-CN-based
tLFIA's
multiplex
guarantees
sensitive
AFB1,
limits
0.0012
ng/mL
(colorimetric
signals),
0.0094
(fluorescent
0.252
(photothermal
signals).
trimode
immunosensor
628-fold
42-fold
that
original
Nb26-based
ELISA
(IC50)
unimodal
(LOD).
This
work
provides
idea
constructing
sensitive,
tolerant
efficient
analytical
platform
rapidly
detecting
AFB1
food.
ACS Applied Materials & Interfaces,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 17, 2025
Alkaline
phosphatase
(ALP)
is
a
biomarker
for
many
diseases,
and
monitoring
its
activity
level
important
disease
diagnosis
treatment.
In
this
study,
we
used
the
microdroplet
technology
combined
with
an
in
situ
laser-induced
polymerization
method
to
prepare
Ag
nanoparticle
(AgNP)
doped
hydrogel
microbeads
(HMBs)
adjustable
pore
sizes
that
allow
small
molecules
enter
while
blocking
large
molecules.
The
AgNPs
embedded
microspheres
can
provide
SERS
activity,
improving
signal
of
diffuse
AgNPs.
A
specific
hydrolysis
reaction
ALP
on
5-bromo-4-chloro-3-indolylphosphate
(BCIP)
was
introduced
itsproduct
5,5′-dibromo-4,4′-dichloro-1H,1H-[2,2']bisindolyl-3,3′-dione
(BCI)
employed
assess
due
highly
resonance
Raman
activity.
sensing
platform
applied
model
serum
evaluate
inhibitors.
assay
showed
higher
sensitivity
than
UV–vis
absorption
spectroscopy,
lowest
detectable
concentration
1.0
×
10–20
M.
addition,
HepG2
cells
evaluated
using
platform,
showing
lower
ALP-expressing
controls
response
hypoxia
iron
metastasis.
This
SERS-activated
HMB
shows
great
potential
detecting
expected
help
analyze
complex
clinical
samples.
ACS Nano,
Год журнала:
2023,
Номер
18(1), С. 1084 - 1097
Опубликована: Дек. 27, 2023
Water
instability
and
sensing
homogeneity
are
the
Achilles'
heel
of
CsPbX3
NPs
in
biological
fluids
application.
This
work
reports
preparation
Mn2+:CsPbCl3@SiO2
yolk–shell
nanoparticles
(YSNPs)
aqueous
solutions
created
through
integration
ligand,
surface,
crystal
engineering
strategies.
The
SN2
reaction
between
4-chlorobutyric
acid
(CBA)
oleylamine
(OAm)
yields
a
zwitterionic
ligand
that
facilitates
dispersion
YSNPs
water,
while
robust
SiO2
shell
enhances
their
overall
stability.
Besides,
Mn2+
doping
not
only
introduces
second
emission
center
but
also
enables
potential
postsynthetic
designability,
leading
to
switching
from
MnO2@YSNPs
with
excellent
oxidase
(OXD)-like
activity.
Theoretical
calculations
reveal
electron
transfer
CsPbCl3
situ
MnO2
adsorption–desorption
process
3,3′,5,5′-tetramethylbenzidine
(TMB)
synergistically
amplify
OXD-like
In
presence
ascorbic
(AA),
Mn4+
(fluorescent
nanozyme)
is
reduced
dissociated,
thereby
inhibiting
activity
triggering
fluorescence
"turn-on/off",
i.e.,
dual-mode
recognition.
Finally,
biomarker
reporting
platform
based
on
fluorescent
nanozyme
constructed
AA
as
reporter
molecule,
accurate
detection
human
serum
alkaline
phosphatase
(ALP)
realized,
demonstrating
vast
perovskites
biosensing.
