ACS Materials Letters,
Journal Year:
2024,
Volume and Issue:
6(3), P. 1059 - 1068
Published: Feb. 22, 2024
Drug
toxicity
and
related
drug-induced
liver
injury
(DILI)
have
become
major
biosafety
issues.
Enzyme-activated
fluorescent
probes
been
demonstrated
as
a
powerful
tool
for
the
diagnosis
of
DILI;
however,
they
suffer
from
diffusive
signal
dilution
interference
with
other
organs,
thus
leading
to
misassessment
drug
inaccurate
diagnosis.
Alkaline
phosphatase
(ALP)
is
an
important
biomarker,
alterations
in
enzyme
level
are
tightly
correlated
severity
damage.
Herein,
enzyme-activated
intramolecular
hydrogen
bond
(IMHB)
enhanced
probe
(TPEG-P)
was
developed
ALP
detection
cells
mice
models
DILI.
Differing
previously
reported
intermolecular
charge
transfer
(ICT)
or
excited-state
proton
(ESIPT)
mechanisms,
TPEG-P
enables
precise
recognition
imaging
only
through
activation
bonds
could
situ
sensitively
detect
varying
degrees
caused
by
toxicity.
This
IMHB
strategy
will
advance
development
bioimaging
future.
ACS Nano,
Journal Year:
2023,
Volume and Issue:
17(19), P. 19359 - 19371
Published: Oct. 2, 2023
The
multimodal
lateral
flow
immunoassay
(LFIA)
has
provided
accurate
and
reliable
results
for
fast
immediate
detection.
Nonetheless,
LFIA
remains
challenging
to
develop
biosensors
with
high
sensitivity
tolerance
matrix
interference
in
agro-food.
In
this
study,
we
developed
a
self-assembled
multivalent
fluorescence-nanobody
(Nb26-EGFP-H6)
16.5-fold
30-fold
higher
affinity
than
monovalent
nanobody
(Nb26).
Based
on
the
Nb26-EGFP-H6,
synthesized
enhanced
immune-probes
Zn-CN@Nb26-EGFP-H6
by
pyrolyzing
oxidizing
an
imidazolating
zeolite
framework-8
(ZIF-8)
obtain
photothermal
metal–carbon
nanomaterials
(Zn-CN)
immobilizing
Nb26-EGFP-H6.
rough
porous
structure
of
Zn-CN
large
surface
area
facilitates
enrichment
immobilization
antibodies.
A
trimodal
(tLFIA)
colorimetric,
fluorescent,
triple
signal
outputs
was
constructed
detection
aflatoxin
B1
(AFB1)
maize.
Attractively,
Zn-CN-based
tLFIA's
multiplex
guarantees
sensitive
AFB1,
limits
0.0012
ng/mL
(colorimetric
signals),
0.0094
(fluorescent
0.252
(photothermal
signals).
trimode
immunosensor
628-fold
42-fold
that
original
Nb26-based
ELISA
(IC50)
unimodal
(LOD).
This
work
provides
idea
constructing
sensitive,
tolerant
efficient
analytical
platform
rapidly
detecting
AFB1
food.
Alkaline
phosphatase
(ALP)
is
a
biomarker
for
many
diseases,
and
monitoring
its
activity
level
important
disease
diagnosis
treatment.
In
this
study,
we
used
the
microdroplet
technology
combined
with
an
in
situ
laser-induced
polymerization
method
to
prepare
Ag
nanoparticle
(AgNP)
doped
hydrogel
microbeads
(HMBs)
adjustable
pore
sizes
that
allow
small
molecules
enter
while
blocking
large
molecules.
The
AgNPs
embedded
microspheres
can
provide
SERS
activity,
improving
signal
of
diffuse
AgNPs.
A
specific
hydrolysis
reaction
ALP
on
5-bromo-4-chloro-3-indolylphosphate
(BCIP)
was
introduced
itsproduct
5,5′-dibromo-4,4′-dichloro-1H,1H-[2,2']bisindolyl-3,3′-dione
(BCI)
employed
assess
due
highly
resonance
Raman
activity.
sensing
platform
applied
model
serum
evaluate
inhibitors.
assay
showed
higher
sensitivity
than
UV–vis
absorption
spectroscopy,
lowest
detectable
concentration
1.0
×
10–20
M.
addition,
HepG2
cells
evaluated
using
platform,
showing
lower
ALP-expressing
controls
response
hypoxia
iron
metastasis.
This
SERS-activated
HMB
shows
great
potential
detecting
expected
help
analyze
complex
clinical
samples.
RSC Advances,
Journal Year:
2023,
Volume and Issue:
13(24), P. 16413 - 16452
Published: Jan. 1, 2023
Heterocycles
are
a
class
of
compounds
that
have
been
found
to
be
potent
inhibitors
alkaline
phosphatase
(AP),
an
enzyme
plays
critical
role
in
various
physiological
processes
such
as
bone
metabolism,
cell
growth
and
differentiation,
has
linked
several
diseases
cancer
osteoporosis.
AP
is
widely
distributed
enzyme,
its
inhibition
considered
therapeutic
strategy
for
the
treatment
these
diseases.
Heterocyclic
inhibit
by
binding
active
site
thereby
inhibiting
activity.
imidazoles,
pyrazoles,
pyridines
studied
potential
therapeutics
cancer,
osteoporosis,
other
However,
development
more
selective
can
used
ongoing
area
research.
Additionally,
study
mechanism
action
heterocyclic
research,
which
could
lead
identification
new
targets
strategies.
The
known
functions
present
multiple
tissues
organs
throughout
body.
This
article
presents
overview
different
types
isoforms,
their
distribution,
roles.
It
also
discusses
structure
AP,
including
hydrolysis
phosphate
groups.
Furthermore,
importance
clinical
marker
liver
disease,
disorders,
emphasized,
well
use
diagnosis
rare
inherited
disorders
hypophosphatasia.
applications
explored.
objective
this
literature
review
examine
function
conditions
diseases,
analyze
structure-activity
relationships
recently
reported
inhibitors.
summarizes
relationship
(SAR)
SAR
studies
revealed
presence
ring,
particularly
pyridine,
pyrimidine,
or
pyrazole
molecule
essential
inhibitory
substitution
pattern
stereochemistry
ring
play
crucial
determining
potency
inhibitor.
Analytical Chemistry,
Journal Year:
2023,
Volume and Issue:
95(4), P. 2356 - 2365
Published: Jan. 16, 2023
Alkaline
phosphatase
(ALP)
and
interleukin-1beta
(IL-1β)
are
crucial
salivary
biomarkers
for
the
diagnosis
of
periodontal
disease
that
harms
tissue
along
with
tooth
loss.
However,
there
has
been
no
way
sensitive
portable
detection
both
in
saliva
multivariate
signal
readout.
In
this
work,
we
design
multicolorimetric
ALP
IL-1β
sensing
platform
based
on
geometrical
transformation
silver
nanoplate
transducer.
By
utilizing
enzymatic
activity
dephosphorylates
p-aminophenol
phosphate
(p-APP)
to
(p-AP),
localized
surface
plasmon
resonance
properties
vary
show
a
distinct
color
change
from
blue
yellow
controlled
seed
triangular
hexagonal,
rounded
pentagonal,
spherical
shape.
The
multicolor
sensor
shows
an
range
0–25
U/L
limit
(LOD)
0.0011
U/L,
which
is
lowest
LOD
demonstrated
date
state-of-the-art
sensor.
Furthermore,
integrate
conventional
ELISA
detect
signaling
it
exhibits
linear
0–250
pg/mL
0.066
pg/mL,
2
orders
magnitude
lower
than
monochromic
(LOD
3.8
pg/mL).
high
selectivity
recovery
100.9%
real
human
proving
its
reliability
suitability
readily
accessible
