Nanoconfined Silver Nanoclusters Combined with X-Shaped DNA Recognizer-Triggered Cascade Amplification for Electrochemiluminescence Detection of APE1

Analytical Chemistry, Год журнала: 2024, Номер 96(28), С. 11383 - 11389

Опубликована: Июль 1, 2024

Apurinic/apyrimidinic endonuclease 1 (APE1), as a vital base excision repair enzyme, is essential for maintaining genomic integrity and stability, its abnormal expression closely associated with malignant tumors. Herein, we constructed an electrochemiluminescence (ECL) biosensor detecting APE1 activity by combining nanoconfined ECL silver nanoclusters (Ag NCs) X-shaped DNA recognizer-triggered cascade amplification. Specifically, the Ag NCs were prepared confined in glutaraldehyde-cross-linked chitosan hydrogel network using one-pot method, resulting strong response exceptional stability comparison discrete NCs. Furthermore, self-assembled recognizers designed detection, which not only improved reaction kinetics due to ordered arrangement of recognition sites but also achieved high sensitivity utilizing amplification strand displacement (SDA) DNAzyme catalysis. As expected, this sensitive detection range 1.0 × 10–3 U·μL–1 10–10 limit 2.21 10–11 U·μL–1, rendering it desirable approach biomarker detection.

Язык: Английский

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Fluorescent Metal Nanoclusters for Explosive Detection: A Review

TrAC Trends in Analytical Chemistry, Год журнала: 2024, Номер 180, С. 117919 - 117919

Опубликована: Авг. 17, 2024

Язык: Английский

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Enzymatic reaction-modulated in-situ formation of nanomaterials and their applications in colorimetric and fluorescent sensing

Coordination Chemistry Reviews, Год журнала: 2024, Номер 508, С. 215787 - 215787

Опубликована: Март 17, 2024

Язык: Английский

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Protein-templated metal nanoclusters for chemical sensing

Frontiers in Analytical Science, Год журнала: 2025, Номер 5

Опубликована: Янв. 22, 2025

Metal nanoclusters (MNCs) possess unique optical properties, discrete energy levels, biocompatibility and photostability, making them pivotal photoluminescent probes in chemical sensing. While substantial work has addressed the synthesis, theoretical studies applications of gold-, copper-, silver-based MNCs, this review introduces fresh perspectives on how nature concentration templates—particularly protein molecules—affect stability sensing capabilities MNCs. We delve into merits using templates for creating highly stable MNCs with tunable photoluminescence (PL), providing a detailed comparison non-protein based systems. This also unveils recent advancements photophysical characteristics protein-templated setting it apart from previous reviews by focusing cutting-edge innovations template influence. Challenges future prospects are highlighted, marking critical pathways upcoming research. not only integrates current knowledge but identifies gaps opportunities covered extensively earlier reviews, such as nuanced effects variation MNCs’ functional properties.

Язык: Английский

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Ratiometric Fluorescence Biosensing of Tandem Biemissive Ag Clusters Boosted by Confined Catalytic DNA Assembly

Analytical Chemistry, Год журнала: 2023, Номер 95(48), С. 17928 - 17936

Опубликована: Ноя. 16, 2023

The reaction kinetics and yield of traditional DNA assembly with a low local concentration in homogeneous solution remain challenging. Exploring confined catalytic (CCDA) is intriguing to boost the rate efficacy for creating rapid sensitive biosensing platforms. A rolling circle amplification (RCA) product containing multiple tandem repeats natural scaffold capable guiding periodic customized functional probes at precise sites. Here, we present RCA-confined CCDA strategy speed up amplifiable conversion ratiometric fluorescent sensing sequence-specific inducer (I*) by using string green-/red-Ag clusters (sgAgCs srAgCs) as two counterbalance emitters. Upon recognition I*, events are operated toehold-mediated strand displacements localized repetitive units, thereby releasing I* recycled signal as-grown RCA concatemer. reactive species increased facilitate rapider dsDNA complex more efficient input-output conversion, on which clustering template sequences sgAgCs srAgCs blocked opened, enabling synthesis but opposite sgAgCs. Thus, fluorescence emission goes up, while go down. With resultant ratio featuring inherent built-in correction, rapid, sensitive, accurate quantification picomolar level achieved. Benefiting from confinement enhance yield, this CCDA-based provides new paradigm developing simple diverse methodologies.

Язык: Английский

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Preparation and application of copper nanoclusters as a fluorescent sensor for sensitive detection of tartrazine

Microchemical Journal, Год журнала: 2024, Номер 207, С. 112146 - 112146

Опубликована: Ноя. 9, 2024

Язык: Английский

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A novel DNA-imprinted polymer with multiplex signal amplification for highly sensitive detection of Ag+

Microchemical Journal, Год журнала: 2024, Номер 199, С. 110113 - 110113

Опубликована: Фев. 5, 2024

Язык: Английский

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β-Cyclodextrin-Stabilized Silver Nanoparticle Production Combined with Loop-Mediated Isothermal Amplification for the Visual Detection of Contagious Pathogens

Micromachines, Год журнала: 2024, Номер 15(3), С. 378 - 378

Опубликована: Март 12, 2024

β-cyclodextrin (β-CD) is a water-soluble, non-toxic, biocompatible, and cage compound that contains six, seven, or eight α-(1–4)-attached D-glucopyranose residues. The hydroxyl group in the β-CD responsible for reduction of metal ions as well stabilizing nanoparticles. In this study, we developed colorimetric assay identifying contagious pathogens such SARS-CoV-2 Enterococcus faecium (E. faecium) via situ development β-CD-stabilized silver nanoparticles (AgNPs). process, LAMP amplicons produced complex with nitrate (LAMP amplicon–Ag+) which was reduced when heated at 65 °C 5 min presence brown color. limit detection determined to be approximately 101 CFU mL−1 10 fg µL−1 E. SARS-CoV-2, respectively. Significantly, examination diseases completed less than 50 min, including process. Owing high sensitivity rapid readout mechanism AgNP-based assay, it anticipated introduced method can efficiently utilized versatile point-of-care testing (POCT) platform molecular diagnostics resource-limited areas.

Язык: Английский

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Leveraging Concentration Imbalance-Driven DNA Circuit as an Operational Amplifier to Enhance the Sensitivity of Hepatitis B Virus DNA Detection with Hybridization-Responsive DNA-Templated Silver Nanoclusters

JACS Au, Год журнала: 2024, Номер 4(6), С. 2323 - 2334

Опубликована: Май 31, 2024

Hepatitis B virus (HBV) infection remains a major global health concern, necessitating the development of sensitive and reliable diagnostic methods. In this study, we propose novel approach to enhance sensitivity HBV DNA detection by leveraging concentration imbalance-driven circuit (CIDDC) as an operational amplifier, coupled with hybridization-responsive DNA-templated silver nanocluster (DNA-AgNCs) nanoprobe named Q·C6-AgNCs. The CIDDC system effectively converts amplifies input into enriched generic single-stranded output, which subsequently triggers fluorescence DNA-AgNCs reporter upon hybridization, generating measurable signal for detection. By incorporating circuit, not only achieved enhanced lower limit 0.11 nM but also demonstrated high specificity single-base mismatch discriminability Additionally, mix-and-detect assay format is simple, user-friendly, isothermal. This innovative strategy holds promise advancing molecular diagnostics facilitating effective management HBV-related diseases.

Язык: Английский

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A ratiometric fluorescence probe based on the N, S co-doped dots and silver nanoclusters for the sensitive detection of Hg2+

Process Safety and Environmental Protection, Год журнала: 2024, Номер 189, С. 267 - 274

Опубликована: Июнь 17, 2024

Язык: Английский

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