BioMetals, Journal Year: 2024, Volume and Issue: unknown
Published: Oct. 8, 2024
Language: Английский
Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(28), P. 11383 - 11389
Published: July 1, 2024
Apurinic/apyrimidinic endonuclease 1 (APE1), as a vital base excision repair enzyme, is essential for maintaining genomic integrity and stability, its abnormal expression closely associated with malignant tumors. Herein, we constructed an electrochemiluminescence (ECL) biosensor detecting APE1 activity by combining nanoconfined ECL silver nanoclusters (Ag NCs) X-shaped DNA recognizer-triggered cascade amplification. Specifically, the Ag NCs were prepared confined in glutaraldehyde-cross-linked chitosan hydrogel network using one-pot method, resulting strong response exceptional stability comparison discrete NCs. Furthermore, self-assembled recognizers designed detection, which not only improved reaction kinetics due to ordered arrangement of recognition sites but also achieved high sensitivity utilizing amplification strand displacement (SDA) DNAzyme catalysis. As expected, this sensitive detection range 1.0 × 10–3 U·μL–1 10–10 limit 2.21 10–11 U·μL–1, rendering it desirable approach biomarker detection.
Language: Английский
Citations
13
Coordination Chemistry Reviews, Journal Year: 2024, Volume and Issue: 508, P. 215787 - 215787
Published: March 17, 2024
Language: Английский
Citations
8
TrAC Trends in Analytical Chemistry, Journal Year: 2024, Volume and Issue: 180, P. 117919 - 117919
Published: Aug. 17, 2024
Language: Английский
Citations
8
Microchemical Journal, Journal Year: 2024, Volume and Issue: 207, P. 112146 - 112146
Published: Nov. 9, 2024
Language: Английский
Citations
5
Analytical Chemistry, Journal Year: 2023, Volume and Issue: 95(48), P. 17928 - 17936
Published: Nov. 16, 2023
The reaction kinetics and yield of traditional DNA assembly with a low local concentration in homogeneous solution remain challenging. Exploring confined catalytic (CCDA) is intriguing to boost the rate efficacy for creating rapid sensitive biosensing platforms. A rolling circle amplification (RCA) product containing multiple tandem repeats natural scaffold capable guiding periodic customized functional probes at precise sites. Here, we present RCA-confined CCDA strategy speed up amplifiable conversion ratiometric fluorescent sensing sequence-specific inducer (I*) by using string green-/red-Ag clusters (sgAgCs srAgCs) as two counterbalance emitters. Upon recognition I*, events are operated toehold-mediated strand displacements localized repetitive units, thereby releasing I* recycled signal as-grown RCA concatemer. reactive species increased facilitate rapider dsDNA complex more efficient input-output conversion, on which clustering template sequences sgAgCs srAgCs blocked opened, enabling synthesis but opposite sgAgCs. Thus, fluorescence emission goes up, while go down. With resultant ratio featuring inherent built-in correction, rapid, sensitive, accurate quantification picomolar level achieved. Benefiting from confinement enhance yield, this CCDA-based provides new paradigm developing simple diverse methodologies.
Language: Английский
Citations
13
Microchemical Journal, Journal Year: 2024, Volume and Issue: 199, P. 110113 - 110113
Published: Feb. 5, 2024
Language: Английский
Citations
4
Micromachines, Journal Year: 2024, Volume and Issue: 15(3), P. 378 - 378
Published: March 12, 2024
β-cyclodextrin (β-CD) is a water-soluble, non-toxic, biocompatible, and cage compound that contains six, seven, or eight α-(1–4)-attached D-glucopyranose residues. The hydroxyl group in the β-CD responsible for reduction of metal ions as well stabilizing nanoparticles. In this study, we developed colorimetric assay identifying contagious pathogens such SARS-CoV-2 Enterococcus faecium (E. faecium) via situ development β-CD-stabilized silver nanoparticles (AgNPs). process, LAMP amplicons produced complex with nitrate (LAMP amplicon–Ag+) which was reduced when heated at 65 °C 5 min presence brown color. limit detection determined to be approximately 101 CFU mL−1 10 fg µL−1 E. SARS-CoV-2, respectively. Significantly, examination diseases completed less than 50 min, including process. Owing high sensitivity rapid readout mechanism AgNP-based assay, it anticipated introduced method can efficiently utilized versatile point-of-care testing (POCT) platform molecular diagnostics resource-limited areas.
Language: Английский
Citations
4
JACS Au, Journal Year: 2024, Volume and Issue: 4(6), P. 2323 - 2334
Published: May 31, 2024
Hepatitis B virus (HBV) infection remains a major global health concern, necessitating the development of sensitive and reliable diagnostic methods. In this study, we propose novel approach to enhance sensitivity HBV DNA detection by leveraging concentration imbalance-driven circuit (CIDDC) as an operational amplifier, coupled with hybridization-responsive DNA-templated silver nanocluster (DNA-AgNCs) nanoprobe named Q·C6-AgNCs. The CIDDC system effectively converts amplifies input into enriched generic single-stranded output, which subsequently triggers fluorescence DNA-AgNCs reporter upon hybridization, generating measurable signal for detection. By incorporating circuit, not only achieved enhanced lower limit 0.11 nM but also demonstrated high specificity single-base mismatch discriminability Additionally, mix-and-detect assay format is simple, user-friendly, isothermal. This innovative strategy holds promise advancing molecular diagnostics facilitating effective management HBV-related diseases.
Language: Английский
Citations
4
Process Safety and Environmental Protection, Journal Year: 2024, Volume and Issue: 189, P. 267 - 274
Published: June 17, 2024
Language: Английский
Citations
4
Sensors and Actuators B Chemical, Journal Year: 2025, Volume and Issue: 427, P. 137222 - 137222
Published: Jan. 5, 2025
Language: Английский
Citations
0