Authorea (Authorea),
Год журнала:
2023,
Номер
unknown
Опубликована: Июль 17, 2023
DNA
logic
programming,
based
on
the
principles
of
base
complementarity
and
Boolean
operations,
exhibits
organizational
structures
computational
rules
similar
to
machine
language.
Consequently,
programming
enables
decision-making,
output
capabilities
consistent
with
language
in
real-world
applications.
In
recent
years,
thinking
behind
has
synergized
life
sciences,
medicine,
various
interdisciplinary
fields,
establishing
an
intelligent
digitized
research
framework.
It
paved
way
for
cutting-edge
facilitated
transition
from
concept
construction.
This
article
provides
overview
achievements
field
biomedical
applications,
focusing
design
implementation
further
exploring
its
advantages
challenges
frontier
areas.
Analytical Chemistry,
Год журнала:
2022,
Номер
94(48), С. 16945 - 16952
Опубликована: Ноя. 23, 2022
MicroRNAs
(miRNAs)
are
considered
as
significant
biomarkers
in
early
diagnosis
and
treatment
of
diseases.
Herein,
an
electrochemical
biosensor
that
uses
ferrocene
(Fc)-functionalized
covalent
organic
frameworks
(COFs),
a
DNA
tetrahedron
nanostructure
(DTN)
biosensing
interface,
target
catalyzed
hairpin
assembly
(CHA)
strategy
has
been
fabricated
successfully
developed
for
the
sensitive
specific
determination
microRNA-21
(miR-21).
The
COF
served
linked
substrate
immobilization
gold
nanoparticles
(AuNPs),
Fc-COOH,
complementary
probe
L1
to
prepare
signal
COF/Au/Fc/L1,
which
large
surface
area,
extraordinary
catalytic
properties,
superior
biocompatibility
amplify
current
signal.
DTN
containing
sequence
H1
at
one
vertex
was
elaborately
designed
construct
interface;
thus,
CHA
could
be
implemented
on
electrode
surface.
In
presence
miR-21,
reaction
between
H2
triggered
produce
great
number
duplex
(H1/H2)
with
sticky
ends.
Then,
COF/Au/Fc/L1
modified
through
hybridization
end
H1/H2,
thereby
obtaining
amplified
Fc
Under
optimal
conditions,
showed
wide
linear
response
ranging
from
1
fM
10
nM
low
detection
limit
0.33
(S/N
=
3).
Meanwhile,
method
acceptable
accuracy
precision
miR-21
human
serum.
ACS Applied Materials & Interfaces,
Год журнала:
2023,
Номер
15(18), С. 21830 - 21842
Опубликована: Апрель 29, 2023
Multiplexed
detection
of
Parkinson's
disease
(PD)
biomarkers
is
great
significance
for
early
diagnosis
and
personalized
treatment.
In
this
study,
we
fabricated
a
robust
surface-enhanced
Raman
scattering-enabled
lab-on-a-chip
(LoC-SERS)
platform
simultaneous
quantification
α-synuclein,
phosphorylated
tau
protein
181,
osteopontin,
osteocalcin.
Herein,
the
antibody-DNA
conjugate
was
designed
to
introduce
catalytic
hairpin
self-assembly
(CHA)
amplification
into
detection.
Au
nano-stars
(AuNSs)
modified
with
reporter
molecules
hairpin-structure
DNA
1
were
applied
as
SERS
nanotags.
Au-coated
silicon
nanocone
array
(Au/SiNCA)
based
on
maskless
plasma
etching-prepared
high-density
Si
(SiNCA)
surface
ion
sputtering
used
capture
substrate
after
modification
2.
Benefitting
from
conjugate-induced
CHA
amplification,
numerous
AuNSs
can
be
connected
Au/SiNCA
surface,
which
significantly
amplify
plasmonic
coupling
effect
ultrasensitive
detection,
limit
less
than
pg/mL
level.
The
application
highly
uniform
endows
LoC-SERS
excellent
analytical
performance,
including
superior
reproducibility,
satisfactory
universality,
high
sensitivity.
addition,
1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine
(MPTP)-induced
PD
mice
model
established,
results
obtained
in
real
sample
analysis
platform,
may
enlightening
exploiting
monitoring.
Frontiers in Analytical Science,
Год журнала:
2023,
Номер
3
Опубликована: Сен. 25, 2023
Ratiometric
strategy
are
an
invaluable
method
that
helps
to
detect
and
quantify
analytes.
This
approach
relies
on
measuring
changes
in
the
ratio
of
two
or
more
signals
improve
accuracy
sensitivity
results.
strategies
widely
used
a
variety
fields
including
biomedical,
environmental
monitoring
food
safety.
It
is
particularly
popular
when
traditional
single-signal
based
detection
methods
not
feasible,
especially
interfering
substances
severely
affect
detection.
In
addition,
ratiometric
have
potential
reliability
analyte
detection,
leading
better
results
complex
environments.
The
article
provides
comprehensive
review
strategy,
focusing
fluorescent
nanoprobes
for
visual
paper
also
discusses
design
two-photon
probes
biomedical
imaging,
synthesis
surface-enhanced
Raman
scattering
imaging
intracellular
analytes,
development
molecularly
imprinted
electrochemical
sensors
electroactive
species,
use
isotopically-labeled
internal
standards
matrix-assisted
laser
desorption/ionization
analysis.
only
each
technique
detail,
its
principles,
advantages,
applications,
limitations,
but
highlights
recent
advances
possible
future
directions.
Chemical Communications,
Год журнала:
2023,
Номер
59(14), С. 1987 - 1990
Опубликована: Янв. 1, 2023
In
this
work,
we
constructed
a
novel
membrane
fusion
strategy
for
extracellular
vesicles
(EVs)
and
red
blood
cell
(RVs).
A
nanoscale
space
is
formed,
which
can
improve
the
efficiency
of
probe
reaction
with
miRNA-21,
allows
in
situ
fluorescence
detection
miRNA-21
EVs.
Advanced Materials,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 19, 2025
Abstract
DNA‐directed
assembly
has
emerged
as
a
versatile
and
powerful
approach
for
constructing
complex
structured
materials.
By
leveraging
the
programmability
of
DNA
nanotechnology,
highly
organized
photonic
systems
can
be
developed
to
optimize
light‐matter
interactions
improved
diagnostics
therapeutic
outcomes.
These
enable
precise
spatial
arrangement
components,
minimizing
material
usage,
simplifying
fabrication
processes.
nanostructures,
such
origami,
provide
robust
platform
building
multifunctional
devices
with
tailored
optical
properties.
This
review
highlights
recent
progress
in
nanomaterials,
focusing
on
their
applications
therapeutics.
It
provides
an
overview
latest
advancements
field,
discussing
principles
assembly,
strategies
functionalizing
blocks,
innovations
design,
resulting
effects
that
drive
these
developments.
The
also
explores
how
architectures
contribute
diagnostic
applications,
emphasizing
potential
create
efficient
effective
specific
healthcare
needs.
