bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 2, 2024
Abstract
The
Drosophila
visual
system
is
a
powerful
model
to
study
the
development
of
neural
circuits.
Projection
neurons
that
relay
information
from
lobula
part
optic
lobe
central
brain
(the
columnar
neurons-LCNs),
are
thought
encode
different
features
relevant
natural
behavior.
There
∼20
classes
LCNs
whose
projections
form
highly
specific,
non-overlapping
synaptic
domains
in
called
glomeruli.
Although
functional
investigations
several
LCN
circuits
have
been
carried
out,
very
little
known
about
their
developmental
origin
and
stem
cell
lineages
generate
subtypes.
To
address
origin,
we
used
single-cell
mRNA
sequencing
define
transcriptome
each
subtype
identified
driver
lines
expressed
specific
throughout
development.
We
show
originate
cells
four
distinct
regions
fly
exhibit
modes
neurogenesis,
including
ventral
dorsal
tips
outer
proliferation
center
(tOPC),
inner
(vtIPC)
(CB).
This
convergence
similar
illustrates
complexity
generating
neuronal
diversity
likely
reflects
evolutionary
detects
feature
influence
behaviors
might
be
species.
Neuron,
Год журнала:
2022,
Номер
110(10), С. 1700 - 1711.e6
Опубликована: Март 14, 2022
Topographic
maps,
the
systematic
spatial
ordering
of
neurons
by
response
tuning,
are
common
across
species.
In
Drosophila,
lobula
columnar
(LC)
neuron
types
project
from
optic
lobe
to
central
brain,
where
each
forms
a
glomerulus
in
distinct
position.
However,
advantages
this
glomerular
arrangement
unclear.
Here,
we
examine
functional
and
relationships
10
glomeruli
using
single-neuron
calcium
imaging.
We
discover
novel
detectors
for
objects
smaller
than
lens
resolution
(LC18)
complex
line
motion
(LC25).
find
that
spatially
clustered
selectivity
looming
versus
drifting
object
ordered
size
tuning
form
topographic
visual
feature
map.
Furthermore,
connectome
analysis
shows
downstream
integrate
sparse
subsets
possible
combinations,
which
biased
encoding
similar
features.
LC
thus
an
explicit
example
topographically
organized
facilitate
circuit
integration.
Abstract
Originally
a
genetic
model
organism,
the
experimental
use
of
Drosophila
melanogaster
has
grown
to
include
quantitative
behavioral
analyses,
sophisticated
perturbations
neuronal
function,
and
detailed
sensory
physiology.
A
highlight
these
developments
can
be
seen
in
context
vision,
where
pioneering
studies
have
uncovered
fundamental
generalizable
principles
processing.
Here
we
begin
with
an
overview
vision-guided
behaviors
common
methods
for
probing
visual
circuits.
We
then
outline
anatomy
physiology
brain
regions
involved
processing,
beginning
at
periphery
ending
descending
motor
control.
Areas
focus
contrast
motion
detection
optic
lobe,
circuits
feature
selectivity,
computations
support
spatial
navigation,
contextual
associative
learning.
Finally,
look
future
fly
neuroscience
discuss
promising
topics
further
study.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Март 17, 2024
Abstract
Animals
are
often
bombarded
with
visual
information
and
must
prioritize
specific
features
based
on
their
current
needs.
The
neuronal
circuits
that
detect
relay
have
been
well-studied.
Yet,
much
less
is
known
about
how
an
animal
adjusts
its
attention
as
goals
or
environmental
conditions
change.
During
social
behaviors,
flies
need
to
focus
nearby
flies.
Here,
we
study
the
flow
of
altered
when
female
Drosophila
enter
aggressive
state.
From
connectome,
identified
three
state-dependent
circuit
motifs
poised
selectively
amplify
response
fly-sized
objects:
convergence
excitatory
inputs
from
neurons
conveying
select
internal
state;
dendritic
disinhibition
feature
detectors;
a
switch
toggles
between
two
detectors.
Using
cell-type-specific
genetic
tools,
together
behavioral
neurophysiological
analyses,
show
each
these
function
during
aggression.
We
reveal
this
same
operate
in
males
courtship
pursuit,
suggesting
disparate
behaviors
may
share
mechanisms.
Our
work
provides
compelling
example
using
connectome
infer
mechanisms
underlie
dynamic
processing
sensory
signals.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 6, 2025
How
specification
mechanisms
that
generate
neural
diversity
translate
into
specific
neuronal
targeting,
connectivity,
and
function
in
the
adult
brain
is
not
understood.
In
medulla
region
of
Drosophila
optic
lobe,
progenitors
different
neurons
a
fixed
order
by
sequentially
expressing
series
temporal
transcription
factors
as
they
age.
Then,
Notch
signaling
intermediate
further
diversifies
progeny.
By
establishing
birth
neurons,
we
found
their
identity
correlates
with
depth
neuropil
targeting
brain,
for
both
local
interneurons
projection
neurons.
We
show
this
identity-dependent
unfolds
early
development
genetically
determined.
leveraging
Electron
Microscopy
reconstruction
fly
determined
synapse
location
lobe
neuropils
find
it
significantly
associated
status.
Moreover,
all
putative
same
predicted
share
similar
location,
indicating
ensembles
layers
encode
visual
functions.
conclusion,
status
can
predict
function,
linking
developmental
patterning
connectivity
functional
features
brain.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Окт. 13, 2023
A
catalog
of
neuronal
cell
types
has
often
been
called
a
"parts
list"
the
brain,
and
regarded
as
prerequisite
for
understanding
brain
function.
In
optic
lobe
Current Biology,
Год журнала:
2020,
Номер
30(13), С. 2508 - 2519.e6
Опубликована: Май 21, 2020
Several
fundamental
aspects
of
motion
vision
circuitry
are
prevalent
across
flies
and
mice.
Both
taxa
segregate
ON
OFF
signals.
For
any
given
spatial
pattern,
detectors
in
both
tuned
to
speed,
selective
for
one
four
cardinal
directions,
modulated
by
catecholamine
neurotransmitters.
These
similarities
represent
conserved,
canonical
properties
the
functional
circuits
computational
algorithms
vision.
Less
is
known
about
feature
detectors,
including
how
receptive
field
differ
from
pathway
or
whether
they
under
neuromodulatory
control
impart
plasticity
detection
salient
objects
a
moving
background.
Here,
we
investigated
19
types
putative
lobula
columnar
(LC)
neurons
optic
lobe
fruit
fly
Drosophila
melanogaster
characterize
divergent
selection.
We
identified
LC12
LC15
as
detectors.
encodes
bars,
whereas
discrete
objects,
mostly
independent
size.
Neither
contrast
polarity,
direction,
highlighting
key
differences
underlying
show
that
onset
background
suppresses
object
responses
LC15.
Surprisingly,
application
octopamine,
which
released
during
flight,
reverses
suppressive
influence
motion,
rendering
LCs
able
track
superimposed
against
motion.
Our
results
provide
comparative
framework
function
modulation
new
insights
into
neuronal
mechanisms
involved
visual
detection.
