Light and Temperature Control the Contribution of Specific DN1 Neurons to Drosophila Circadian Behavior

Current Biology, Год журнала: 2010, Номер 20(7), С. 600 - 605

Опубликована: Апрель 1, 2010

Язык: Английский

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Sodium Leak Channels in Neuronal Excitability and Rhythmic Behaviors

Neuron, Год журнала: 2011, Номер 72(6), С. 899 - 911

Опубликована: Дек. 1, 2011

Язык: Английский

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RNA-seq analysis of Drosophila clock and non-clock neurons reveals neuron-specific cycling and novel candidate neuropeptides

PLoS Genetics, Год журнала: 2017, Номер 13(2), С. e1006613 - e1006613

Опубликована: Фев. 9, 2017

Locomotor activity rhythms are controlled by a network of ~150 circadian neurons within the adult Drosophila brain. They subdivided based on their anatomical locations and properties. We profiled transcripts "around clock" from three key groups with different functions. also non-circadian outgroup, dopaminergic (TH) neurons. have cycling but fewer than clock as well low expression poor gene transcripts. This suggests that TH do not canonical is driven brain systemic cues. The surprisingly diverse in overall patterns, which include known putative novel neuropeptides. Even phase distributions distinct, indicating regulatory principles govern transcript oscillations. surprising cell-type diversity parallels functional heterogeneity

Язык: Английский

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A Series of Suppressive Signals within the Drosophila Circadian Neural Circuit Generates Sequential Daily Outputs

Neuron, Год журнала: 2017, Номер 94(6), С. 1173 - 1189.e4

Опубликована: Май 25, 2017

Язык: Английский

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Analysis of functional neuronal connectivity in theDrosophilabrain

Journal of Neurophysiology, Год журнала: 2012, Номер 108(2), С. 684 - 696

Опубликована: Апрель 27, 2012

Drosophila melanogaster is a valuable model system for the neural basis of complex behavior, but an inability to routinely interrogate physiologic connections within central networks fly brain remains fundamental barrier progress in field. To address this problem, we have introduced simple method measuring functional connectivity based on independent expression mammalian P2X2 purinoreceptor and genetically encoded Ca(2+) cAMP sensors separate defined subsets neurons adult brain. We show that such capable specifically rendering sets excitable by pulses bath-applied ATP manner compatible with high-resolution imaging putative follower neurons. Furthermore, establish approach sufficiently sensitive detection excitatory modulatory deep larval brains. This technically facile can now be used wild-type mutant genetic backgrounds neuronal governing wide range behaviors fly. effectiveness suggests similar methods using appropriate heterologous receptors might adopted other widely systems.

Язык: Английский

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Improving Water Productivity of Wheat-Based Cropping Systems in South Asia for Sustained Productivity

Advances in agronomy, Год журнала: 2014, Номер unknown, С. 157 - 258

Опубликована: Янв. 1, 2014

Язык: Английский

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Circadian and feeding cues integrate to drive rhythms of physiology in Drosophila insulin-producing cells

Genes & Development, Год журнала: 2016, Номер 30(23), С. 2596 - 2606

Опубликована: Дек. 1, 2016

Circadian clocks regulate much of behavior and physiology, but the mechanisms by which they do so remain poorly understood. While cyclic gene expression is thought to underlie metabolic rhythms, little known about cycles in cellular physiology. We found that Drosophila insulin-producing cells (IPCs), are located pars intercerebralis lack an autonomous circadian clock, functionally connected central clock circuit via DN1 neurons. Insulin mediates output regulating rhythmic a (sxe2) fat body. Patch clamp electrophysiology reveals IPCs display clock-regulated daily rhythms firing event frequency bursting proportion under light:dark conditions. The activity sxe2 additionally regulated feeding, as demonstrated night feeding-induced changes IPC characteristics levels These findings indicate circuit-level regulation metabolism support role for integrating control

Язык: Английский

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Dual PDF Signaling Pathways Reset Clocks Via TIMELESS and Acutely Excite Target Neurons to Control Circadian Behavior

PLoS Biology, Год журнала: 2014, Номер 12(3), С. e1001810 - e1001810

Опубликована: Март 18, 2014

Molecular circadian clocks are interconnected via neural networks. In Drosophila, PIGMENT-DISPERSING FACTOR (PDF) acts as a master network regulator with dual functions in synchronizing molecular oscillations between disparate PDF(+) and PDF(−) pacemaker neurons controlling neuron output. Yet the mechanisms by which PDF not clear. We demonstrate that genetic inhibition of protein kinase A (PKA) clock can phenocopy mutants while activated PKA partially rescue receptor mutants. subunit transcripts also under control non-PDF DN1p neurons. To address core target PDF, we rescued per arrhythmic per01 induced high amplitude rhythms component TIMELESS (TIM) per-less Complete loss or results reduced TIM levels flies. how impacts output, focally applied to found it acutely depolarizes increases firing rates Surprisingly, these effects presence an adenylate cyclase inhibitor, yet persist inhibition. have provided evidence for signaling mechanism resets synchronizes demonstrates acute direct excitatory effect on neuronal The identification suggests is multimodal integrator cell autonomous clock, environmental light, signaling. Moreover, data reveal bifurcation PKA-dependent PKA-independent output effects. Taken together, our provide cellular basis resetting

Язык: Английский

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Circadian rhythms in neuronal activity propagate through output circuits

Nature Neuroscience, Год журнала: 2016, Номер 19(4), С. 587 - 595

Опубликована: Фев. 29, 2016

Язык: Английский

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A Neural Network Underlying Circadian Entrainment and Photoperiodic Adjustment of Sleep and Activity in Drosophila

Journal of Neuroscience, Год журнала: 2016, Номер 36(35), С. 9084 - 9096

Опубликована: Авг. 31, 2016

A sensitivity of the circadian clock to light/dark cycles ensures that biological rhythms maintain optimal phase relationships with external day. In animals, neuron network (CCNN) driving sleep/activity receives light input from multiple photoreceptors, but how these photoreceptors modulate CCNN components is not well understood. Here we show Hofbauer-Buchner eyelets differentially two classes ventral lateral neurons (LNvs) within Drosophila CCNN. The antagonize Cryptochrome (CRY)- and compound-eye-based photoreception in large LNvs while synergizing CRY-mediated small LNvs. Furthermore, interact subsets “evening cells” adjust timing evening peak activity a day length-dependent manner. Our work identifies peptidergic connection between group cells critical for seasonal adjustment rhythms. SIGNIFICANCE STATEMENT clocks have evolved orchestrate behavior metabolism. Consistent requires entrainment solar day, process an organism9s health. Light are most important cue clocks, system uses link timekeeping cycle. How information photorecptors integrated into support results establish HB impacts physiology neuronal subgroups. This pathway, together compound eyes, precisely times flies under long summer days. provide mechanistic model transduction integration system, identifying new unexpected motifs network.

Язык: Английский

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