The Neuronal Circuit of the Dorsal Circadian Clock Neurons in Drosophila melanogaster DOI Creative Commons
Nils Reinhard, Frank K. Schubert, Enrico Bertolini

и другие.

Frontiers in Physiology, Год журнала: 2022, Номер 13

Опубликована: Апрель 29, 2022

Drosophila's dorsal clock neurons (DNs) consist of four clusters (DN1as, DN1ps, DN2s, and DN3s) that largely differ in size. While the DN1as DN2s encompass only two neurons, DN1ps ∼15 DN3s comprise ∼40 per brain hemisphere. In comparison to well-characterized lateral (LNs), neuroanatomy function DNs are still not clear. Over past decade, numerous studies have addressed their role fly's circadian system, leading several sometimes divergent results. Nonetheless, these agreed important fine-tune activity under light temperature cycles play essential roles linking output from LNs downstream control sleep metabolism. Here, we used Flybow specific split-GAL4 lines, trans-Tango, recently published fly connectome (called hemibrain) describe morphology greater detail, including synaptic connections other non-clock neurons. We show some DN groups heterogenous. certain strongly connected with LNs, others mainly signal circuits clock. Among latter mushroom body central complex tubercle bulb neurosecretory cells pars intercerebralis, unidentified partners. This heterogeneity may explain conflicting results previously found about functionality. Most importantly, identify putative novel communication centers network: one fiber bundle superior protocerebrum running toward anterior optic hub posterior protocerebrum. Both invaded by might an instrumental network.

Язык: Английский

A connectome and analysis of the adult Drosophila central brain DOI Creative Commons
Louis K. Scheffer, C. Shan Xu, Michał Januszewski

и другие.

eLife, Год журнала: 2020, Номер 9

Опубликована: Сен. 3, 2020

The neural circuits responsible for animal behavior remain largely unknown. We summarize new methods and present the circuitry of a large fraction brain fruit fly Drosophila melanogaster . Improved include procedures to prepare, image, align, segment, find synapses in, proofread such data sets. define cell types, refine computational compartments, provide an exhaustive atlas examples many them novel. detailed consisting neurons their chemical most central brain. make public simplify access, reducing effort needed answer circuit questions, linking defined by our analysis with genetic reagents. Biologically, we examine distributions connection strengths, motifs on different scales, electrical consequences compartmentalization, evidence that maximizing packing density is important criterion in evolution fly’s

Язык: Английский

Процитировано

1003
The connectome of the adult Drosophila mushroom body provides insights into function DOI Creative Commons
Feng Li, Jack Lindsey, Elizabeth C. Marin

и другие.

eLife, Год журнала: 2020, Номер 9

Опубликована: Дек. 14, 2020

Making inferences about the computations performed by neuronal circuits from synapse-level connectivity maps is an emerging opportunity in neuroscience. The mushroom body (MB) well positioned for developing and testing such approach due to its conserved architecture, recently completed dense connectome, extensive prior experimental studies of roles learning, memory, activity regulation. Here, we identify new components MB circuit Drosophila, including visual input output neurons (MBONs) with direct connections descending neurons. We find unexpected structure sensory inputs, transfer information different modalities MBONs, modulation that dopaminergic (DANs). provide insights into circuitry used integrate outputs, between central complex inputs DANs, feedback MBONs. Our results a foundation further theoretical work.

Язык: Английский

Процитировано

349
Complete Connectomic Reconstruction of Olfactory Projection Neurons in the Fly Brain DOI Creative Commons
Alexander Shakeel Bates, Philipp Schlegel, Ruairí J.V. Roberts

и другие.

Current Biology, Год журнала: 2020, Номер 30(16), С. 3183 - 3199.e6

Опубликована: Июль 2, 2020

Nervous systems contain sensory neurons, local projection and motor neurons. To understand how these building blocks form whole circuits, we must distil broad classes into neuronal cell types describe their network connectivity. Using an electron micrograph dataset for entire Drosophila melanogaster brain, reconstruct the first complete inventory of olfactory projections connecting antennal lobe, insect analog mammalian bulb, to higher-order brain regions in adult animal brain. We then connect this extant data literature, providing synaptic-resolution "holotypes" both heavily investigated previously unknown types. Projection neurons are approximately twice as numerous reported by light level studies; stereotyped, but not identical, synapse numbers between hemispheres. The lateral horn, cortical amygdala, is main target information has been shown guide innate behavior. Here, find new connectivity motifs, including axo-axonic feedback, inhibition axons a large population convergence different inputs, non-olfactory inputs memory-related feedback onto third-order These features less prominent mushroom body calyx, piriform cortex center associative memory. Our work provides neuroanatomical platform future studies system.

Язык: Английский

Процитировано

178
Information flow, cell types and stereotypy in a full olfactory connectome DOI Creative Commons
Philipp Schlegel, Alexander Shakeel Bates, Tomke Stürner

и другие.

eLife, Год журнала: 2021, Номер 10

Опубликована: Май 25, 2021

The

Язык: Английский

Процитировано

146
Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster DOI Creative Commons
Nils Eckstein, Alexander Shakeel Bates, Andrew Champion

и другие.

Cell, Год журнала: 2024, Номер 187(10), С. 2574 - 2594.e23

Опубликована: Май 1, 2024

High-resolution electron microscopy of nervous systems has enabled the reconstruction synaptic connectomes. However, we do not know sign for each connection (i.e., whether a is excitatory or inhibitory), which implied by released transmitter. We demonstrate that artificial neural networks can predict transmitter types presynapses from micrographs: network trained to six transmitters (acetylcholine, glutamate, GABA, serotonin, dopamine, octopamine) achieves an accuracy 87% individual synapses, 94% neurons, and 91% known cell across D. melanogaster whole brain. visualize ultrastructural features used prediction, discovering subtle but significant differences between phenotypes. also analyze distributions brain find neurons develop together largely express only one fast-acting GABA). hope our publicly available predictions act as accelerant neuroscientific hypothesis generation fly.

Язык: Английский

Процитировано

121
Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons DOI Creative Commons
Darya Task, Chun‐Chieh Lin, Alina Vulpe

и другие.

eLife, Год журнала: 2022, Номер 11

Опубликована: Апрель 20, 2022

olfactory neurons have long been thought to express only one chemosensory receptor gene family. There are two main families in

Язык: Английский

Процитировано

106
Humidity sensors that alert mosquitoes to nearby hosts and egg-laying sites DOI
Willem J. Laursen, Gonzalo Budelli,

Ruocong Tang

и другие.

Neuron, Год журнала: 2023, Номер 111(6), С. 874 - 887.e8

Опубликована: Янв. 13, 2023

Язык: Английский

Процитировано

46
Common principles for odour coding across vertebrates and invertebrates DOI

Kara A Fulton,

David Zimmerman,

Aravi Samuel

и другие.

Nature reviews. Neuroscience, Год журнала: 2024, Номер 25(7), С. 453 - 472

Опубликована: Май 28, 2024

Язык: Английский

Процитировано

22
A Drosophila computational brain model reveals sensorimotor processing DOI Creative Commons
Philip K. Shiu, Gabriella R Sterne, Nico Spiller

и другие.

Nature, Год журнала: 2024, Номер 634(8032), С. 210 - 219

Опубликована: Окт. 2, 2024

Язык: Английский

Процитировано

22
Systematic annotation of a complete adult male Drosophila nerve cord connectome reveals principles of functional organisation DOI Open Access
Elizabeth C. Marin, Billy J Morris, Tomke Stürner

и другие.

Опубликована: Июль 22, 2024

Nervous systems function as ensembles of neurons communicating via synaptic connections, and a functional understanding nervous requires extensive knowledge their connectomes. In companion paper (Takemura et al., 2023), we describe the acquisition complete fruit fly nerve cord connectome, first for an animal that can walk or fly. Here, to efficiently navigate appreciate biological significance this categorise name nearly all systematically link them experimental literature. We employ system hierarchical coarse annotations group similar across midline segments, then define systematic cell types sensory neurons, intrinsic ascending non-motor efferent neurons. Stereotyped arrays neuroblasts generate related neuron populations called hemilineages repeat segments cord. confirm larval-born from given hemilineage generally express same neurotransmitter but find earlier born often different one. match over 35% intrinsic, ascending, defining serial sets which were crucial typing motor assign modality 5000 cluster by connectivity, identify serially homologous layered organisation likely corresponding peripheral topography. Finally, present selected examples circuits predicated on programmatic analysis VNC connectome. Our are critical analysing structure descending input output, both described in third (Cheong 2023). These being released part neuprint.janelia.org clio.janelia.org web applications also serve basis connectome through dedicated tools paper.

Язык: Английский

Процитировано

18