Dopamine modulation of sensory processing and adaptive behavior in flies DOI Creative Commons

K.P. Siju,

Jean‐François De Backer, Ilona C Grunwald Kadow

et al.

Cell and Tissue Research, Journal Year: 2021, Volume and Issue: 383(1), P. 207 - 225

Published: Jan. 1, 2021

Abstract Behavioral flexibility for appropriate action selection is an advantage when animals are faced with decisions that will determine their survival or death. In order to arrive at the right decision, evaluate information from external environment, internal state, and past experiences. How these different signals integrated modulated in brain, how context- state-dependent behavioral controlled poorly understood questions. Studying molecules help convey integrate such neural circuits important way approach Many years of work model organisms have shown dopamine a critical neuromodulator (reward based) associative learning. However, recent findings vertebrates invertebrates demonstrated complexity heterogeneity dopaminergic neuron populations functional implications many adaptive behaviors survival. For example, neurons can sensory information, states, learned experience decision making circuitry. Several advances methodologies availability synaptic level connectome whole-brain circuitry Drosophila melanogaster make fly attractive system study roles behavior. particular, learning memory center—the mushroom body—is richly innervated by enable it multi-modal according state context, modulate decision-making

Language: Английский

A connectome and analysis of the adult Drosophila central brain DOI Creative Commons
Louis K. Scheffer, C. Shan Xu, Michał Januszewski

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Sept. 3, 2020

The neural circuits responsible for animal behavior remain largely unknown. We summarize new methods and present the circuitry of a large fraction brain fruit fly Drosophila melanogaster . Improved include procedures to prepare, image, align, segment, find synapses in, proofread such data sets. define cell types, refine computational compartments, provide an exhaustive atlas examples many them novel. detailed consisting neurons their chemical most central brain. make public simplify access, reducing effort needed answer circuit questions, linking defined by our analysis with genetic reagents. Biologically, we examine distributions connection strengths, motifs on different scales, electrical consequences compartmentalization, evidence that maximizing packing density is important criterion in evolution fly’s

Language: Английский

Citations

996
The connectome of the adult Drosophila mushroom body provides insights into function DOI Creative Commons
Feng Li, Jack Lindsey, Elizabeth C. Marin

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Dec. 14, 2020

Making inferences about the computations performed by neuronal circuits from synapse-level connectivity maps is an emerging opportunity in neuroscience. The mushroom body (MB) well positioned for developing and testing such approach due to its conserved architecture, recently completed dense connectome, extensive prior experimental studies of roles learning, memory, activity regulation. Here, we identify new components MB circuit Drosophila, including visual input output neurons (MBONs) with direct connections descending neurons. We find unexpected structure sensory inputs, transfer information different modalities MBONs, modulation that dopaminergic (DANs). provide insights into circuitry used integrate outputs, between central complex inputs DANs, feedback MBONs. Our results a foundation further theoretical work.

Language: Английский

Citations

348
Complete Connectomic Reconstruction of Olfactory Projection Neurons in the Fly Brain DOI Creative Commons
Alexander Shakeel Bates, Philipp Schlegel, Ruairí J.V. Roberts

et al.

Current Biology, Journal Year: 2020, Volume and Issue: 30(16), P. 3183 - 3199.e6

Published: July 2, 2020

Nervous systems contain sensory neurons, local projection and motor neurons. To understand how these building blocks form whole circuits, we must distil broad classes into neuronal cell types describe their network connectivity. Using an electron micrograph dataset for entire Drosophila melanogaster brain, reconstruct the first complete inventory of olfactory projections connecting antennal lobe, insect analog mammalian bulb, to higher-order brain regions in adult animal brain. We then connect this extant data literature, providing synaptic-resolution "holotypes" both heavily investigated previously unknown types. Projection neurons are approximately twice as numerous reported by light level studies; stereotyped, but not identical, synapse numbers between hemispheres. The lateral horn, cortical amygdala, is main target information has been shown guide innate behavior. Here, find new connectivity motifs, including axo-axonic feedback, inhibition axons a large population convergence different inputs, non-olfactory inputs memory-related feedback onto third-order These features less prominent mushroom body calyx, piriform cortex center associative memory. Our work provides neuroanatomical platform future studies system.

Language: Английский

Citations

176
Information flow, cell types and stereotypy in a full olfactory connectome DOI Creative Commons
Philipp Schlegel, Alexander Shakeel Bates, Tomke Stürner

et al.

eLife, Journal Year: 2021, Volume and Issue: 10

Published: May 25, 2021

The

Language: Английский

Citations

145
Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster DOI Creative Commons
Nils Eckstein, Alexander Shakeel Bates, Andrew Champion

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(10), P. 2574 - 2594.e23

Published: May 1, 2024

High-resolution electron microscopy of nervous systems has enabled the reconstruction synaptic connectomes. However, we do not know sign for each connection (i.e., whether a is excitatory or inhibitory), which implied by released transmitter. We demonstrate that artificial neural networks can predict transmitter types presynapses from micrographs: network trained to six transmitters (acetylcholine, glutamate, GABA, serotonin, dopamine, octopamine) achieves an accuracy 87% individual synapses, 94% neurons, and 91% known cell across D. melanogaster whole brain. visualize ultrastructural features used prediction, discovering subtle but significant differences between phenotypes. also analyze distributions brain find neurons develop together largely express only one fast-acting GABA). hope our publicly available predictions act as accelerant neuroscientific hypothesis generation fly.

Language: Английский

Citations

120
Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons DOI Creative Commons
Darya Task, Chun‐Chieh Lin, Alina Vulpe

et al.

eLife, Journal Year: 2022, Volume and Issue: 11

Published: April 20, 2022

olfactory neurons have long been thought to express only one chemosensory receptor gene family. There are two main families in

Language: Английский

Citations

104
Humidity sensors that alert mosquitoes to nearby hosts and egg-laying sites DOI
Willem J. Laursen, Gonzalo Budelli,

Ruocong Tang

et al.

Neuron, Journal Year: 2023, Volume and Issue: 111(6), P. 874 - 887.e8

Published: Jan. 13, 2023

Language: Английский

Citations

45
Common principles for odour coding across vertebrates and invertebrates DOI

Kara A Fulton,

David Zimmerman,

Aravi Samuel

et al.

Nature reviews. Neuroscience, Journal Year: 2024, Volume and Issue: 25(7), P. 453 - 472

Published: May 28, 2024

Language: Английский

Citations

22
A Drosophila computational brain model reveals sensorimotor processing DOI Creative Commons
Philip K. Shiu, Gabriella R Sterne, Nico Spiller

et al.

Nature, Journal Year: 2024, Volume and Issue: 634(8032), P. 210 - 219

Published: Oct. 2, 2024

Language: Английский

Citations

22
Functional connectomics spanning multiple areas of mouse visual cortex DOI Creative Commons
J. Alexander Bae,

Mahaly Baptiste,

Maya R. Baptiste

et al.

Nature, Journal Year: 2025, Volume and Issue: 640(8058), P. 435 - 447

Published: April 9, 2025

Abstract Understanding the brain requires understanding neurons’ functional responses to circuit architecture shaping them. Here we introduce MICrONS connectomics dataset with dense calcium imaging of around 75,000 neurons in primary visual cortex (VISp) and higher areas (VISrl, VISal VISlm) an awake mouse that is viewing natural synthetic stimuli. These data are co-registered electron microscopy reconstruction containing more than 200,000 cells 0.5 billion synapses. Proofreading a subset yielded reconstructions include complete dendritic trees as well local inter-areal axonal projections map up thousands cell-to-cell connections per neuron. Released open-access resource, this includes tools for retrieval analysis 1,2 . Accompanying studies describe its use comprehensive characterization cell types 3–6 , synaptic level connectivity diagram cortical column 4 uncovering cell-type-specific inhibitory can be linked gene expression 4,7 Functionally, identify new computational principles how information integrated across space 8 characterize novel neuronal invariances 9 bring structure function together uncover general principle between excitatory within 10,11

Language: Английский

Citations

10