Biomolecules,
Год журнала:
2024,
Номер
15(1), С. 32 - 32
Опубликована: Дек. 30, 2024
Background:
In
this
study,
we
investigated
the
role
of
extracellular
vesicles
(EVs)
in
pathogenesis
Congenital
Zika
Syndrome
(CZS).
Previous
studies
have
highlighted
EVs
intercellular
communication
and
modulation
biological
processes
during
viral
infections,
motivating
our
in-depth
analysis.
Our
objective
was
to
identify
specific
molecular
signatures
patients
with
CZS,
focusing
on
their
potential
as
biomarkers
cellular
pathways
affected
by
infection.
Methods:
We
conducted
advanced
proteomic
metabolomic
analyses
using
mass
spectrometry
for
protein
metabolite
identification.
were
isolated
from
CZS
patient
samples
control
groups
Izon
qEV
size-exclusion
chromatography
columns.
Results:
The
analyzed
presented
distinct
profiles
CZS.
Proteomic
analysis
revealed
significant
alterations
proteins,
suggesting
involvement
PI3K-AKT-mTOR
pathway,
while
metabolomics
metabolites
related
critical
virus
pathogenesis.
These
findings
suggest
a
key
pathway
regulating
infection
indicate
communication.
Additionally,
results
identified
capable
aiding
early
diagnosis
assessing
disease
progression.
Conclusions:
This
study
demonstrates
that
play
crucial
identification
highlights
possible
therapeutic
target,
providing
new
opportunities
development
more
effective
treatment
strategies
significantly
advance
understanding
underscore
need
further
investigations
techniques
validate
explore
these
targets.
Extracellular Vesicles and Circulating Nucleic Acids,
Год журнала:
2024,
Номер
5(1), С. 44 - 63
Опубликована: Янв. 30, 2024
Cell
membrane-derived
vesicles
(CMVs)
are
particles
generated
from
living
cells,
including
extracellular
(EVs)
and
artificial
(aEVs)
prepared
cell
membranes.
CMVs
possess
considerable
potential
in
drug
delivery,
regenerative
medicine,
immunomodulation,
disease
diagnosis,
Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Фев. 22, 2024
Gastric
cancer
(GC)
is
a
malignant
neoplasm
originating
from
the
epithelial
cells
of
gastric
mucosa.
The
pathogenesis
GC
intricately
linked
to
tumor
microenvironment
within
which
reside.
Tumor-associated
macrophages
(TAMs)
primarily
differentiate
peripheral
blood
monocytes
and
can
be
broadly
categorized
into
M1
M2
subtypes.
M2-type
TAMs
have
been
shown
promote
growth,
tissue
remodeling,
angiogenesis.
Furthermore,
they
actively
suppress
acquired
immunity,
leading
poorer
prognosis
reduced
tolerance
chemotherapy.
Exosomes,
contain
myriad
biologically
active
molecules
including
lipids,
proteins,
mRNA,
noncoding
RNAs,
emerged
as
key
mediators
communication
between
TAMs.
exchange
these
via
exosomes
markedly
influence
consequently
impact
progression.
Recent
studies
elucidated
correlation
various
clinicopathological
parameters
GC,
such
size,
differentiation,
infiltration
depth,
lymph
node
metastasis,
TNM
staging,
highlighting
pivotal
role
in
development
metastasis.
In
this
review,
we
aim
comprehensively
examine
bidirectional
TAMs,
implications
alterations
on
immune
escape,
invasion,
metastasis
targeted
therapeutic
approaches
for
efficacy
potential
drug
resistance
strategies.
Frontiers in Aging Neuroscience,
Год журнала:
2024,
Номер
16
Опубликована: Фев. 8, 2024
Vascular
dementia
(VD)
is
a
prevalent
cognitive
disorder
among
the
elderly.
Its
pathological
mechanism
encompasses
neuronal
damage,
synaptic
dysfunction,
vascular
abnormalities,
neuroinflammation,
and
oxidative
stress,
others.
In
recent
years,
extracellular
vesicles
(EVs)
derived
from
mesenchymal
stem
cells
(MSCs)
have
garnered
significant
attention
as
an
emerging
therapeutic
strategy.
Current
research
indicates
that
MSC-derived
(MSC-EVs)
play
pivotal
role
in
both
diagnosis
treatment
of
VD.
Thus,
this
article
delves
into
advancements
MSC-EVs
VD,
discussing
mechanisms
by
which
EVs
influence
pathophysiological
processes
These
form
theoretical
foundation
for
their
neuroprotective
effect
VD
treatment.
Additionally,
highlights
potential
applications
diagnosis.
conclusion,
present
promising
innovative
strategy
With
rigorous
ongoing
innovation,
concept
can
transition
practical
clinical
treatment,
providing
more
effective
options
patients.
Molecular Biomedicine,
Год журнала:
2024,
Номер
5(1)
Опубликована: Ноя. 21, 2024
Abstract
Extracellular
vesicles
(EVs)
are
cell-derived
with
a
phospholipid
bilayer
measuring
50–150
nm
in
diameter
demonstrated
therapeutic
potentials.
Limitations
such
as
the
natural
biodistribution
(mainly
concentrated
liver
and
spleen)
short
plasma
half-life
of
EVs
present
significant
challenges
to
their
clinical
translation.
In
recent
years,
growing
research
indicated
that
engineered
enhanced
targeting
lesion
sites
have
markedly
promoted
efficacy.
However,
there
is
dearth
systematic
knowledge
on
advances
engineering
for
targeted
delivery.
Herein,
we
provide
an
overview
mechanisms,
techniques,
translations
applications.
Enrichment
at
may
be
achieved
through
recognition
tissue
markers,
pathological
changes,
circumvention
mononuclear
phagocyte
system
(MPS).
Alternatively,
external
stimuli,
including
magnetic
fields
ultrasound,
also
employed.
EV
techniques
fulfill
functions
includes
genetic
engineering,
membrane
fusion,
chemical
modification
physical
modification.
A
comparative
statistical
analysis
was
conducted
elucidate
discrepancies
between
diverse
size,
morphology,
stability,
efficacy
vitro
vivo.
Additionally,
summary
registered
trials
utilizing
from
2010
2023
has
been
provided,
full
discussion
perspectives.
This
review
provides
comprehensive
mechanisms
associated
delivery
applications
advocate
further
explorations
accelerate
Journal of Nanobiotechnology,
Год журнала:
2025,
Номер
23(1)
Опубликована: Март 8, 2025
The
liver,
functioning
as
an
endocrine
organ,
secretes
a
variety
of
substances
that
influence
the
activities
other
body
organs.
Conversely,
molecules
generated
by
organs
such
bone,
gut,
and
adipose
tissue
can
also
impact
liver
function.
Accumulating
evidence
suggests
bidirectional
communication
between
However,
research
on
how
extracellular
vesicles
(EVs),
which
transport
active
molecular
mediators,
contribute
to
this
interorgan
is
still
in
its
nascent
stages.
EVs
are
capable
transporting
functional
molecules,
including
lipids,
nucleic
acids,
proteins,
thereby
affecting
recipient
cells
across
different
at
biological
level.
This
review
examines
role
facilitating
cardiovascular
system,
pancreas,
brain,
lungs,
kidneys,
tissue.
It
explores
their
potential
disease
treatment
highlights
challenges
understanding
EV-mediated
interactions.
contribution
mediator-carrying
two-way
remains
area
ongoing
investigation.
Future
will
provide
more
comprehensive
theoretical
foundation
clarify
precise
mechanisms
governing
organs,
pinpoint
medical
targets,
expand
application
within
realm
precision
medicine.
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 25, 2025
Extracellular
vesicles
(EVs)
play
crucial
roles
in
intercellular
communication
and
hold
great
promise
as
biomarkers
for
noninvasive
disease
diagnosis.
Intensive
research
efforts
have
been
devoted
to
discovering
the
EV
subpopulations
responsible
specific
functions
or
with
enhanced
effectiveness
markers,
through
extensive
purification
content
analysis.
However,
their
high
heterogeneity
size
cargo
composition
poses
significant
challenges
reaching
such
goals.
Isolation
methods
like
ultracentrifugation
size-exclusion
chromatography,
well
analysis
approaches
polymerase
chain
reaction
enzyme-linked
immunosorbent
assay,
made
contributions
improving
our
understanding
of
biology.
Nonetheless,
these
face
limitations
isolation
efficiency,
purity,
detection
sensitivity
specificity
due
issues
large
sample
consumption,
unsatisfactory
insufficient
resolution
subtyping.
Microfluidic
technology
presents
promising
solutions
challenges,
leveraging
intrinsic
capabilities
precise
flow
external
energy
field
manipulation,
compartmentalization,
signal
enhancement
at
micro-
nanoscale.
Hence,
this
review
summarizes
recent
developments
microfluidics-enabled
analysis,
paying
special
attention
unique
microfluidic
features
exploited.
Strategies
viscoelastic
inertial
flow,
fluid
mixing,
external-field-assisted
purification,
compartmentalization
micro/nanostructures
enhancing
detection,
are
examined.
Furthermore,
current
potential
future
directions
discussed
inspire
advancements
rapidly
developing
field.
Biomarker Research,
Год журнала:
2024,
Номер
12(1)
Опубликована: Окт. 12, 2024
Abstract
Liver
fibrosis,
a
chronic
and
long-term
disease,
can
develop
into
hepatocellular
carcinoma
(HCC)
ultimately
lead
to
liver
failure.
Early
diagnosis
effective
treatment
still
face
significant
challenges.
inflammation
leads
fibrosis
through
continuous
activation
of
hepatic
stellate
cells
(HSCs)
the
accumulation
immune
cells.
Intracellular
communication
among
various
is
important
for
mediating
inflammatory
response
during
fibrogenesis.
Extracellular
vesicles
(EVs),
which
are
lipid
bilayer
membrane-enclosed
particles
naturally
secreted
by
cells,
make
great
contributions
cell-cell
transport
bioactive
molecules.
Nearly
all
that
participate
in
release
EVs
loaded
with
lipids,
proteins,
nucleic
acids.
from
hepatocytes,
stem
involved
microenvironment
fibrosis.
Recently,
an
increasing
number
extracellular
vesicle-based
clinical
applications
have
emerged,
providing
promising
cell-free
diagnostic
therapeutic
tools
because
their
crucial
role
immunomodulation
pathogenesis.
The
advantages
therapies
include
stability,
biocompatibility,
low
cytotoxicity,
minimal
immunogenicity,
highlight
potential
drug
delivery
specific
treatments
In
this
review,
we
summarize
complex
biological
functions
pathogenesis
evaluate
International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
26(1), С. 225 - 225
Опубликована: Дек. 30, 2024
The
global
issue
of
aging
populations
has
become
increasingly
prominent,
thus
the
research
and
development
for
anti-aging
therapies
to
assure
longevity
as
well
ameliorate
age-related
complications
is
put
high
on
agenda.
young
humoral
milieu
been
substantiated
impart
youthful
characteristics
aged
cells
or
organs.
Extracellular
vesicles
(EVs)
are
a
heterogeneous
group
cell-derived
membrane-limited
structures
that
serve
couriers
proteins
genetic
material
regulate
intercellular
communication.
Of
note,
EVs
appeared
be
an
indispensable
component
blood
in
prolonging
lifespans,
circulating
have
indicated
mediate
beneficial
effect
aging.
Human
umbilical
cord
mesenchymal
stem
(HUCMSC-EVs),
isolated
from
youngest
adult
cell
source,
speculated
reproduce
function
partially
revitalize
numerous
organs
old
animals.
Robust
evidence
suggested
HUCMSC-EVs
muti-target
therapeutic
agents
combating
alleviating
degenerative
disorders.
Here,
we
provide
comprehensive
overview
effects
brain,
heart,
vasculature,
kidney,
muscle,
bone,
other
Furthermore,
critically
discuss
current
investigation
engineering
strategies
HUCMSC-EVs,
intending
unveil
their
full
potential
field
research.
