Lympho-Hematopoietic Microenvironments and Fish Immune System DOI Creative Commons
A. Zapata

Biology, Год журнала: 2022, Номер 11(5), С. 747 - 747

Опубликована: Май 13, 2022

In the last 50 years information on fish immune system has increased importantly, particularly that species of marked commercial interest (i.e., salmonids, cods, catfish, sea breams), occupy a key position in vertebrate phylogenetical tree Agnatha, Chondrichtyes, lungfish) or represent consolidated experimental models, such as zebrafish medaka. However, most obtained was based genetic sequence analysis with little no cellular basis responses. Although jawed contain thymus and lympho-hematopoietic organs equivalents to mammalian bone marrow, few studies have accounted for presumptive relationships between organization these cell microenvironments known capabilities system. current review, we analyze this topic providing on: (1) The origins T B lymphopoiesis Agnatha fish; (2) remarkable teleost (3) occurrence numerous, apparently unrelated housing progenitors and, presumably, lymphopoiesis; (4) existence immunological memory absence germinal centers.

Язык: Английский

Main Components of Fish Immunity: An Overview of the Fish Immune System DOI Creative Commons
Doaa M. Mokhtar, Giacomo Zaccone, Alessio Alesci

и другие.

Fishes, Год журнала: 2023, Номер 8(2), С. 93 - 93

Опубликована: Фев. 5, 2023

Cellular immune responses consist of innate and adaptive cell-mediated mechanisms, where all leukocyte subpopulations are included. Among these vital processes such as cytotoxicity phagocytosis. The main cellular constituents the fish system macrophages, granulocytes, dendritic cells, NK cytotoxic T cells. This review provides latest information on defense mechanisms an overview function mucosal in maintaining general health fish. Here, we discuss fundamental ideas that underpin teleosts, well cells molecules play a role responses. Moreover, cytokine pathways teleosts have been reported to focus several kinds associated immunity. Importantly, also antigen processing presentation. knowledge here will enable better understanding, determination, modulation responsible for protective immunity, thus consequently improving aquaculture.

Язык: Английский

Процитировано

165

Fish disease and intestinal microbiota: A close and indivisible relationship DOI
Diana Medina‐Félix, Estefanía Garibay‐Valdez, Francisco Vargas‐Albores

и другие.

Reviews in Aquaculture, Год журнала: 2022, Номер 15(2), С. 820 - 839

Опубликована: Ноя. 8, 2022

Abstract The gut microbiota is currently one of the most studied ‘organs’ in animals, and fish are no exception. A complex diversity microbes, including bacteria, archaea, yeast fungus, constitute microbiota, creating a interaction with their host accomplishing multiple beneficial functions, such as food digestion, nutrient absorption, immune system, endocrine stress response. microbiota–pathogen protects by mounting colonization resistance, competing for nutrients space. Changes balance community could affect structure homeostasis, inducing dysbiosis. In addition, invading pathogens can induce dysbiosis evading host's defence barriers, acquiring from host, using metabolites produced producing toxins. this regard, understanding interactions within essential to prevent pathogen establishment host. Hence, review describes close indivisible relationships between that lead disease.

Язык: Английский

Процитировано

78

Phytogenic Bioactive Compounds Shape Fish Mucosal Immunity DOI Creative Commons
Joana P. Firmino, Jorge Galindo‐Villegas, Felipe E. Reyes‐López

и другие.

Frontiers in Immunology, Год журнала: 2021, Номер 12

Опубликована: Июнь 18, 2021

Aquaculture growth will unavoidably involve the implementation of innovative and sustainable production strategies, being functional feeds among most promising ones. A wide spectrum phytogenics, particularly those containing terpenes organosulfur compounds, are increasingly studied in aquafeeds, due to their promoting, antimicrobial, immunostimulant, antioxidant, anti-inflammatory sedative properties. This trend relies on importance mucosal barrier fish defense. Establishing phytogenics’ mode action tissues is for further use safe administration. Although impact phytogenics upon immunity has been extensively approached, studies fail addressing mechanisms underlying pharmacological effects. Unstandardized testing as an extended practice also questions reproducibility safety such studies, limiting at commercial scale. The information presented herein provides insight immune responses suggesting action, ultimately encouraging reliable reproducible research novel feed additives aquafeeds. For proper screening, characterization optimization we encourage evaluation purified compounds using vitro systems before moving forward vivo trials. formulation with combinations previously characterized recommended avoid bacterial resistance. To improve delivery overcome limitations associated volatility susceptibility degradation, encapsulation advisable. Besides, newer approaches dedicated methodologies needed elucidate pharmacokinetics depth.

Язык: Английский

Процитировано

84

Immunity of the intestinal mucosa in teleost fish DOI
Michał Stosik, Beata Tokarz‐Deptuła, Wiesław Deptuła

и другие.

Fish & Shellfish Immunology, Год журнала: 2023, Номер 133, С. 108572 - 108572

Опубликована: Янв. 28, 2023

Язык: Английский

Процитировано

43

Cold-blooded vertebrates evolved organized germinal center–like structures DOI Open Access
Yasuhiro Shibasaki, Sergey Afanasyev, Álvaro Fernández-Montero

и другие.

Science Immunology, Год журнала: 2023, Номер 8(90)

Опубликована: Ноя. 1, 2023

Germinal centers (GCs) or analogous secondary lymphoid microstructures (SLMs) are thought to have evolved in endothermic species. However, living representatives of their ectothermic ancestors can mount potent antibody responses upon infection immunization, despite the apparent lack SLMs these cold-blooded vertebrates. How and where adaptive immune induced species absence GCs remain poorly understood. Here, we infected a teleost fish (trout) with parasite Ichthyophthirius multifiliis (Ich) identified formation large aggregates highly proliferating IgM + B cells CD4 T cells, contiguous splenic melanomacrophage (MMCs). Most MMC-associated (M-LAs) contained numerous antigen (Ag)–specific cells. Analysis heavy chain CDR3 repertoire microdissected M-LAs non–M-LA areas revealed that most frequent cell clones after Ich were shared only within animals. These represented polyclonal which Ag-specific clonal expansion occurred. M-LA–associated expressed high levels activation-induced cytidine deaminase underwent significant apoptosis, somatic hypermutation Igμ genes occurred prevalently Our findings demonstrate ectotherms organized GC-like roles. Moreover, our results also point primordially conserved mechanisms by mammalian develop function.

Язык: Английский

Процитировано

41

The adaptive microbiome hypothesis and immune interactions in amphibian mucus DOI Creative Commons
Douglas C. Woodhams,

Julia A. McCartney,

Jenifer B. Walke

и другие.

Developmental & Comparative Immunology, Год журнала: 2023, Номер 145, С. 104690 - 104690

Опубликована: Март 29, 2023

Язык: Английский

Процитировано

34

Host species and habitat shape fish-associated bacterial communities: phylosymbiosis between fish and their microbiome DOI Creative Commons
Javad Sadeghi, Subba Rao Chaganti, Timothy B. Johnson

и другие.

Microbiome, Год журнала: 2023, Номер 11(1)

Опубликована: Ноя. 20, 2023

While many studies have reported that the structure of gut and skin microbiota is driven by both species-specific habitat-specific factors, relative importance host-specific versus environmental factors in wild vertebrates remains poorly understood. The aim this study was to determine diversity composition fish skin, gut, surrounding water bacterial communities (hereafter referred as microbiota) assess extent which host habitat phylogeny predict similarity. Skin swabs samples from 334 belonging 17 species were sampled three Laurentian Great Lakes (LGLs) habitats (Detroit River, Lake Erie, Ontario). We also collected filtered at time collection. analyzed community using 16S metabarcoding tested for variation.We found distinct microbiota, although more closely resembled microbiota. (sample location), habitat, diet, shape promote divergence or convergence Since significantly affected (separately effects), we phylosymbiosis pairwise phylogenetic distance dissimilarity. significant effects on dissimilarity, consistent with perhaps reflecting longstanding co-evolutionary relationship between their microbiomes.Analyzing mucus across diverse complex natural ecosystems such LGLs provides insights into potential microbiome, ultimately health, host. Video Abstract.

Язык: Английский

Процитировано

29

Microbiomes in the context of developing sustainable intensified aquaculture DOI Creative Commons
Marlene Lorgen-Ritchie,

Tamsyn Uren Webster,

Jamie McMurtrie

и другие.

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Июнь 23, 2023

With an ever-growing human population, the need for sustainable production of nutritional food sources has never been greater. Aquaculture is a key industry engaged in active development to increase line with this while remaining terms environmental impact and promoting good welfare health farmed species. Microbiomes fundamentally underpin animal health, being part their digestive, metabolic defense systems, latter case protecting against opportunistic pathogens environment. The potential manipulate microbiome advantage enhancing intriguing prospect that gained considerable traction recent years. In review we first set out what known about role aquaculture systems across phylogenetic spectrum cultured animals, from invertebrates finfish. view reducing footprint tightening biological physical control, investment “closed” on rise, but little how microbial these closed affect organisms. Through comparisons microbiomes dynamics phylogenetically distinct animals different focus communities functionality order identify features within be harnessed optimizing healthy intensified support future aquaculture.

Язык: Английский

Процитировано

28

Efficacy of heat‐killed and formalin‐killed vaccines against Tilapia tilapinevirus in juvenile Nile tilapia (Oreochromis niloticus) DOI
Thao Thu, Pattanapon Kayansamruaj, Suwimon Taengphu

и другие.

Journal of Fish Diseases, Год журнала: 2021, Номер 44(12), С. 2097 - 2109

Опубликована: Сен. 3, 2021

Tilapia tilapinevirus (also known as tilapia lake virus, TiLV) is considered to be a new threat the global industry. The objective of this study was develop simple cell culture-based heat-killed (HKV) and formalin-killed (FKV) vaccines for prevention disease caused by TiLV. fish were immunized with 100 µl either HKV or FKV intraperitoneal injection each vaccine containing 1.8 × 106 TCID50- inactivated virus. A booster vaccination carried out at 21-day post-vaccination (dpv) using same protocol. then challenged lethal dose TiLV 28 dpv. expression five immune genes (IgM, IgD, IgT, CD4 CD8) in head kidney spleen experimental assessed 14 21 dpv again after TiLV-specific IgM responses measured ELISA time points. results showed that both conferred significant protection, relative percentage survival 71.3% 79.6% FKV, respectively. Significant up-regulation IgT observed vaccinated dpv, while IgM, IgD increased receiving point. After vaccination, CD8 transcripts significantly HKV, but not FKV. Both induced specific response serum mucus. In summary, are promising injectable Nile tilapia.

Язык: Английский

Процитировано

48

Temporal changes in skin and gill microbiomes of Atlantic salmon in a recirculating aquaculture system – Why do they matter? DOI Creative Commons
Marlene Lorgen-Ritchie,

Michael Clarkson,

Lynn Chalmers

и другие.

Aquaculture, Год журнала: 2022, Номер 558, С. 738352 - 738352

Опубликована: Май 14, 2022

Mucosal surfaces are key components of teleost health, providing defence against opportunistic pathogens and other insults. Maintaining the integrity mucosal their associated microbial communities, especially gill skin that have large surface areas exposed to environment is essential. Production Atlantic salmon in land-based recirculating aquaculture systems (RAS) has increased significantly recent years as it allows greater control over stability which fish reared, reduces water demand minimises environmental impacts. However, little known about impact RAS upon temporal dynamics microbiomes. In this study we examined communities mucus, mucus rearing throughout freshwater (FW) production, at 1-week 4-weeks following transfer seawater (SW) open cage production using 16S rRNA sequencing. Microbial diversity richness reared a system were temporally dynamic. Dynamics similar two tissues, some extent also mirrored surrounding water. Dysbiosis indicated by an abrupt decline during FW coincided with increase relative abundance taxa belonging RAS-biofilter-associated nitrogen-cycling genus Hydrogenophaga tank was observed mucus. Extensive overlap core between but host-specific cores non-existent dysbiotic event all present Diversity remained stable transition from SW, distinct community composition environments. Although closely controlled, significant variation could be temperature well levels CO2 nitrogen compounds, reflecting increasing biological load within time. The results presented here suggest that, terms microbiomes, dysbiosis may occur both time, capability recover.

Язык: Английский

Процитировано

32