Therapeutic Effects of Nanocoating of Apitoxin (Bee Venom) and Polyvinyl Alcohol Supplemented with Zinc Oxide Nanoparticles

Pharmaceutics, Год журнала: 2025, Номер 17(2), С. 172 - 172

Опубликована: Янв. 28, 2025

Background/Objectives: Bee venom (BV), as a natural product, is one of the foundations pharmaceutical industry, through which many diseases, including serious ones, can be effectively treated. The BV nanofilm an effective antidote delivered into human body to target affected area and address issue without major side effects. In this study, we investigated intriguing therapeutic effects apitoxin (bee venom) used in isolation, combined with powerful properties zinc oxide nanoparticles. Methods Results: was evaluated using Fourier transform infrared (FTIR) spectroscopy X-ray diffraction (XRD). extract analyzed mass spectrometry (MS), identified 84 active components present at varying concentrations. treated both polyvinyl alcohol (PVA) nanoparticles (ZNPs) increase intensity OH CH2 groups enhance dispersion C=O. has demonstrated anti-type 2 diabetes activity by inhibiting α-amylase α-glucosidase, are starch-degrading enzymes. mixture BV, PVA, ZNPs, exhibited highest antidiabetic IC50 values 30.33 μg/mL 5.55 for inhibition compared 51.69 µg/mL 7.30 respectively. also showed higher anti-inflammatory red blood cell (RBC) hemolysis, 16.99 comparison 72.99 alone. broad-spectrum antimicrobial activity, targeting Gram-positive (Staphylococcus aureus ATCC 6538 Bacillus subtilis 6633) Gram-negative bacteria (Salmonella typhi 6539, Escherichia coli 8739). Furthermore, increased antioxidant recorded 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging effect 4.26 19.43 found more toxic liver tissue (HepG2 line) than nanofilm, 18.5 ± 0.08 52.27 0.7 μg/mL, displayed toxicity (2.3%) 97.7% viability 250 0.09% 99.9% same concentration. Conclusions: emerges promising alternative medicine, offering innovative solution treating various diseases its high concentration therapeutically compounds effortless delivery.

Язык: Английский

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Whey protein hydrolysate from camel mitigates hypoxia-induced renal and gill damage in Nile Tilapia: Effects on stress, HIF-α, apoptosis, inflammation, and metabolic pathways

Aquaculture, Год журнала: 2025, Номер unknown, С. 742256 - 742256

Опубликована: Фев. 1, 2025

Язык: Английский

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Ammodaucus leucotrichus Coss. & Dur. Attenuates acrylamide-induced hepatic injury through enhancing autophagy and inhibiting endoplasmic reticulum stress and apoptotic signaling

Journal of Functional Foods, Год журнала: 2024, Номер 115, С. 106090 - 106090

Опубликована: Март 7, 2024

In our study, we investigated the potential of Ammodaucus leucotrichus Coss. & Dur. (Al) seed extract and essential oil (Al oil) to mitigate acrylamide-induced liver injury in rats. We administered (100 200 mg/kg), (0.25 mL/kg) or reference drug (silymarin, 100 mg/kg) for four days. On fourth day, was induced by acrylamide (25 mg/kg, PO), continuing treatment seven more assessed enzymes, oxidative endoplasmic reticulum (ER) stress markers, apoptotic autophagy conducted histopathological examinations. The ameliorated functional structural alterations caused acrylamide, reduced ER stress, inhibited apoptosis, enhanced autophagy. high-dose showed effects like silymarin, potentially attributed its phenolic contents. These activities indicated hepatoprotective A. against xenobiotics-induced damage, supporting their use as nutraceuticals stress-related disorders.

Язык: Английский

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Acrylamide-targeting renal miR−21a−5p/Fibrotic and miR122-5p/ inflammatory signaling pathways and the role of a green approach for nano-zinc detected via in silico and in vivo approaches

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Июль 17, 2024

Introduction: Any disruption in renal function can have cascading effects on overall health. Understanding how a heat-born toxicant like acrylamide (ACR) affects kidney tissue is vital for realizing its broader implications systemic Methods: This study investigated the ACR-induced damage mechanisms, particularly focusing regulating role of miR-21a-5p/fibrotic and miR-122-5p/inflammatory signaling pathways via targeting Timp-3 TP53 proteins an In silico preliminary study. Besides, assessment, oxidative status, protein profile, expression biomarkers (Timp-1, Keap-1, Kim-1, P53, TNF-α, Bax, Caspase3) were assessed 60-day experiment. The examination was additionally extended to explore potential protective green-synthesized zinc oxide nanoparticles (ZNO-MONPs). A four-group experiment including control, ZNO-MONPs (10 mg/kg b.wt.), ACR (20 + established encompassing biochemical, histological, molecular levels. further protein-binding ability ZNO MONPs inactivate caspase-3, TNFRS-1A. Results: significantly reduced as evidenced by increased serum creatinine, uric acid, albumin, stress markers. stress, apoptosis, inflammationare revealed biochemical tests, gene expression, presence apoptotic nuclei microscopically. Also, docking binding affinity between ACR-BCL-2 glutathione-synthetase, elucidating mechanisms through which induces damage. Notably, against Zn levels tissues ACR-exposed rats restored those treated with ZNO-MONPs. conclusion, this establishes efficacy mitigating disturbances functions, inflammation, apoptosis. findings shed light renoprotective activity nanomaterials, offering insights into novel therapeutic approaches countering

Язык: Английский

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Thymol’s modulation of cellular macromolecules, oxidative stress, DNA damage, and NF-kB/Caspase-3 signaling in the liver of imidacloprid-exposed rats

Environmental Toxicology and Pharmacology, Год журнала: 2024, Номер 109, С. 104492 - 104492

Опубликована: Июнь 3, 2024

Язык: Английский

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Effects of dietary acrylamide on kidney and liver health: Molecular mechanisms and pharmacological implications

Toxicology Reports, Год журнала: 2024, Номер 14, С. 101859 - 101859

Опубликована: Дек. 10, 2024

Acrylamide (AA) has raised concerns throughout the world in recent years because of its potential negative effects on human health. Numerous researches humans and animals have connected a high dietary exposure to AA possible risk cancer. Additionally, higher consumption acrylamide also been associated with dysfunctioning various organ systems from nervous system reproductive system. is primarily metabolised into glycidamide inside body which gets accumulated different tissues including kidney liver, chronic this can lead nephrotoxicity hepatotoxicity through molecular mechanisms. This review summarizes sources, formation metabolism along mechanisms such as oxidative stress, inflammation, DNA damage, autophagy, mitochondrial dysfunction morphological changes nephron hepatocytes exerts deleterious effect liver causing hepatotoxicity. animal cellular studies that demonstrate AA-induced Lastly, article emphasizes underlying protective pharmacological interventions against induced nephrotoxicity.

Язык: Английский

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Restorative effects of gallic acid against sub-chronic hepatic toxicity of co-exposure to zinc oxide nanoparticles and arsenic trioxide in male rats

Heliyon, Год журнала: 2023, Номер 9(6), С. e17326 - e17326

Опубликована: Июнь 1, 2023

This study aimed to assess the effect of zinc oxide nanoparticles (ZNPs) and/or arsenic trioxide (ATO) exposure on liver adult male Sprague Dawley rats. Moreover, probable ameliorative impact gallic acid (GA) against ZNPs and ATO-induced hepatotoxicity possible underlying mechanisms were evaluated.Sixty rats distributed into six groups. The 1st 2nd groups orally given distilled water (1 ml/kg) 20 mg GA/kg b. wt, respectively. 3rd 4th 100 ZNPs/kg wt 8 ATO/kg 5th group was co-administered ATO at doses mentioned above. last one ZNPs, ATO, GA earlier described doses. All tested compounds once a day for 60 successive days. Then, serum levels alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate (AST), total, direct, indirect bilirubin, triglycerides, total cholesterol, HDL, VLDL, LDL estimated. hepatic content malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx) evaluated. Bcl-2 Bax's reactive proteins immunohistochemically detected, Zn As residual patterns in tissues assessed.ZNPs, ZNPs+ATO-exposed showed significantly (P < 0.001) elevated AST (219%, 233%, 333%), ALT (300%, 400%, 475%), ALP (169%, 205%, 294%), bilirubin (42%, 68%, 109%) compared control ones. On other hand, declined SOD (58%, 49%, 43%) GPx (70%, 63%, 56%) but increased MDA (133%, 150%, 224%) recorded ZNPs+ATO exposed rats, respectively, relative significant decrease (28%, 33%, 23%) elevation Bax (217%, 267%, 236%) immunoreactivities These findings consistent with microscopic alterations architecture accumulation As. Furthermore, notable hyperlipidemic condition following exposure. contrary, notably reduced enzymes Additionally, markedly improved ZNPs+ATO-afforded tissue damage apoptotic events.Overall, oral dosing mitigated negative effects by improving antioxidant defense system controlling changes.

Язык: Английский

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Thymoquinone Abrogates Acrylamide-Induced Cerebellar Toxicity via Modulation of Nuclear Factor Erythroid 2-Related Factor 2/Nuclear Factor Kappa B Signaling, Oxidative Neuroinflammation, and Neuroapoptosis in Rats

Journal of Medicinal Food, Год журнала: 2024, Номер 27(11), С. 1062 - 1069

Опубликована: Сен. 25, 2024

Acrylamide (ACR) is an obligate human neurotoxicant ubiquitously produced and found in foods processed at high temperature. There increasing public health concern regarding its probable carcinogenic potential. Its prevailing toxicity mechanism oxidative inflammation apoptosis. Herein, we explored whether thymoquinone (TQ), a bioactive quinone

Язык: Английский

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Biofabricated zinc oxide nanoparticles mitigate acrylamide-induced immune toxicity and modulate immune-related genes and microRNA in rats

Naunyn-Schmiedeberg s Archives of Pharmacology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 16, 2024

Язык: Английский

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